Schistosome miracidial host finding: synopsis and a molecular approach
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
1995
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | IV, 137 S. Ill., graph. Darst. |
Internformat
MARC
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| 100 | 1 | |a Haberl, Bernhard |e Verfasser |4 aut | |
| 245 | 1 | 0 | |a Schistosome miracidial host finding |b synopsis and a molecular approach |c von Bernhard Haberl |
| 264 | 1 | |c 1995 | |
| 300 | |a IV, 137 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 502 | |a Erlangen-Nürnberg, Univ., Diss., 1995 | ||
| 650 | 0 | 7 | |a Erkennung |0 (DE-588)4328500-4 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Glykokonjugate |0 (DE-588)4131221-1 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Wirt |0 (DE-588)4132477-8 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Immunoblot |0 (DE-588)4209100-7 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Pärchenegel |0 (DE-588)4173097-5 |2 gnd |9 rswk-swf |
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| 943 | 1 | |a oai:aleph.bib-bvb.de:BVB01-007070201 | |
Datensatz im Suchindex
| _version_ | 1807502292349353984 |
|---|---|
| adam_text |
1.
INTRODUCTION
.
1
1.1.
THE
PARASITE
.
4
1.1.1.
THE
SCHISTOSOME
MIRACIDIUM
.
8
1.2.
THE
SNAIL
HOSTS
.
10
1.3.
SCHISTOSOME
-
SNAIL
INTERACTIONS
.
12
2.
MIRACIDIAL
HOST-FINDING
-
A
REVIEW
.
15
2.1.
BEHAVLORAL
MECHANISMS
.
15
2.2.
CHEMICAL
HOST
CUES
.
26
2.3.
HOST
SPECIFICITY
.
37
EXPERIMENTAL
SECTION:
ISOLATION
AND
PARTIAL
CHARACTERISATION
OF
THE
GLYCOCONJUGATES
STIMULATING
HOST-FINDING
BEHAVIOR
IN
S.
MANSONI
MIRACIDIA
3.
MATERIAL
AND
METHODS
.
44
3.1.
PARASITES
AND
HOSTS
.
44
3.2.
BIOASSAY
AND
EXPERIMENTAL
CONDITIONS
.
45
3.3.
PREPARATION
OF
SCW
.
46
3.4.
FRACTIONATLON
OF
SCW
.
47
3.4.1.
MOLECULAR
FILTRATION
.
47
3.4.2.
ANION
EXCHANGE
CHROMATOGRAPHY
.
47
3.4.3.
SIZE
EXCLUSION
CHROMATOGRAPHY
.
48
3.4.4.
FRACTIONATION
BY
CHEMICAL
AND
ENZYMATIC
METHODS
.
48
3.4.5.
BLOCKING
OF
CARBOHYDRATE
SIGNAL
STRUCTURES
WITH
LECTINS
.
49
3.5.
ANALYTICAL
METHODS
.
50
3.5.1.
POLYACRYLAMIDE
GEL
ELECTROPHORESIS
.
50
3.5.1.1.
PROTEIN
STAINING
.
52
3.5.1.2.
GLYCOPROTEIN
STAINING
.
53
3.5.2
AGAROSE
GEL
ELECTROPHORESIS
.
54
3.5.3.
WESTERN
BLOTTING
.
54
3.5.3.1.
PROTEIN
STAINING
.
55
3.5.3.2.
GLYCOPROTEIN
STAINING
.
56
3.5.4.
DIFFERENTIATION
OF
CARBOHYDRATES
WITH
LECTINS
.
57
3.5.5.
DETERMINATION
OF
NEUTRAL
SUGARS
.
58
3.5.6.
DETERMINATION
OF
NEUTRAL
HEXOSES
.
58
3.5.7.
DETERMINATION
OF
PROTEIN
CONTENT
.
58
3.5.8.
DYNAMIC
LIGHT
SCATTERING
.
59
2.6.
STATISTICAL
ANALYSIS
.
59
4.
RESULTS
.
60
4.1.
ANALYSIS
OF
SCW
.
60
4.1.1.
ELECTROPHORETIC
SEPARATION
OF
SCW
.
60
4.1.2.
SDS-PAGE
AFTER
HYDROLYSIS
OF
PROTEINS
.
63
4.1.3.
SDS-PAGE
AFTER
SS-ELIMINATION
.
65
4.1.4.
WESTERN
BLOT
AND
IMMUNOLOGICAL
STAINING
FOR
CARBOHYDRATES
.
67
4.1.5.
SIZE
EXCLUSION
CHROMATOGRAPHY
.
69
4.1.6.
DETERMINATION
OF
MOLECULAR
SIZE
BY
DYNAMIC
LIGHT
SCATTERING
.
72
4.2.
ISOLATION
OF
THE
EFFECTIVE
COMPONENT
IN
SCW
BY
ION
EXCHANGE
.
73
4.3.
ENZYMATIC
AND
CHEMICAL
DEGRADATION
OF THE
EFFECTIVE
FRACTION
OF
SCW
86
4.4.
ANALYSIS
OF
CARBOHYDRATES
USING
LECTINS
.
89
4.4.1.
THE
EFFECT
OF
LECTINS
ON
MIRACIDIAL
HOST-FINDING
BEHAVIOR
.
89
4.4.2.
ANALYSIS
OF
CARBOHYDRATES
IN
THE
EFFECTIVE
FRACTION
OF
SCW
.
91
5.
DISCUSSION
.
96
5.1.
SCW
AND
EFFECTIVE
COMPONENTS
.
96
5.1.1.
MACROMOLECULAR
COMPOUNDS
IN
SCW
.
97
5.1.2.
THE
ROLE
OF
SMALL
MOLECULAR
COMPOUNDS
.
102
5.2.
ISOLATION
OF
THE
EFFECTIVE
COMPONENT
FROM
SCW
.
103
5.3.
IS
THERE
ONLY
ONE
SNAIL
HOST
SIGNAL
USED
BY
MIRACIDIA?
.
105
5.4.
SPECIFICITY
IN
MIRACIDIAL
HOST-FINDING
.
106
5.5.
PROSPECTS
AND
REMAINING
QUESTIONS
.
107
6.
SUMMARY
.
109
7.
ZUSAMMENFASSUNG
.
111
8.
REFERENCES
.
114 |
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| discipline | Biologie |
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| genre_facet | Hochschulschrift |
| id | DE-604.BV010600562 |
| illustrated | Illustrated |
| indexdate | 2024-08-16T00:35:12Z |
| institution | BVB |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-007070201 |
| oclc_num | 645871530 |
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| owner_facet | DE-29 DE-29T DE-91 DE-BY-TUM DE-12 DE-355 DE-BY-UBR DE-11 DE-188 |
| physical | IV, 137 S. Ill., graph. Darst. |
| publishDate | 1995 |
| publishDateSearch | 1995 |
| publishDateSort | 1995 |
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| spelling | Haberl, Bernhard Verfasser aut Schistosome miracidial host finding synopsis and a molecular approach von Bernhard Haberl 1995 IV, 137 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Erlangen-Nürnberg, Univ., Diss., 1995 Erkennung (DE-588)4328500-4 gnd rswk-swf Glykokonjugate (DE-588)4131221-1 gnd rswk-swf Wirt (DE-588)4132477-8 gnd rswk-swf Immunoblot (DE-588)4209100-7 gnd rswk-swf Pärchenegel (DE-588)4173097-5 gnd rswk-swf Schistosoma mansoni (DE-588)4317794-3 gnd rswk-swf Miracidium (DE-588)4536182-4 gnd rswk-swf Wirtsfindung (DE-588)4424392-3 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Pärchenegel (DE-588)4173097-5 s Wirt (DE-588)4132477-8 s Erkennung (DE-588)4328500-4 s Glykokonjugate (DE-588)4131221-1 s Immunoblot (DE-588)4209100-7 s DE-604 Schistosoma mansoni (DE-588)4317794-3 s Miracidium (DE-588)4536182-4 s Wirtsfindung (DE-588)4424392-3 s DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=007070201&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Haberl, Bernhard Schistosome miracidial host finding synopsis and a molecular approach Erkennung (DE-588)4328500-4 gnd Glykokonjugate (DE-588)4131221-1 gnd Wirt (DE-588)4132477-8 gnd Immunoblot (DE-588)4209100-7 gnd Pärchenegel (DE-588)4173097-5 gnd Schistosoma mansoni (DE-588)4317794-3 gnd Miracidium (DE-588)4536182-4 gnd Wirtsfindung (DE-588)4424392-3 gnd |
| subject_GND | (DE-588)4328500-4 (DE-588)4131221-1 (DE-588)4132477-8 (DE-588)4209100-7 (DE-588)4173097-5 (DE-588)4317794-3 (DE-588)4536182-4 (DE-588)4424392-3 (DE-588)4113937-9 |
| title | Schistosome miracidial host finding synopsis and a molecular approach |
| title_auth | Schistosome miracidial host finding synopsis and a molecular approach |
| title_exact_search | Schistosome miracidial host finding synopsis and a molecular approach |
| title_full | Schistosome miracidial host finding synopsis and a molecular approach von Bernhard Haberl |
| title_fullStr | Schistosome miracidial host finding synopsis and a molecular approach von Bernhard Haberl |
| title_full_unstemmed | Schistosome miracidial host finding synopsis and a molecular approach von Bernhard Haberl |
| title_short | Schistosome miracidial host finding |
| title_sort | schistosome miracidial host finding synopsis and a molecular approach |
| title_sub | synopsis and a molecular approach |
| topic | Erkennung (DE-588)4328500-4 gnd Glykokonjugate (DE-588)4131221-1 gnd Wirt (DE-588)4132477-8 gnd Immunoblot (DE-588)4209100-7 gnd Pärchenegel (DE-588)4173097-5 gnd Schistosoma mansoni (DE-588)4317794-3 gnd Miracidium (DE-588)4536182-4 gnd Wirtsfindung (DE-588)4424392-3 gnd |
| topic_facet | Erkennung Glykokonjugate Wirt Immunoblot Pärchenegel Schistosoma mansoni Miracidium Wirtsfindung Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=007070201&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT haberlbernhard schistosomemiracidialhostfindingsynopsisandamolecularapproach |