Capillary electrophoresis: methods and potentials
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Book |
| Language: | German |
| Published: |
Braunschweig ; Wiesbaden
Vieweg
1996
|
| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Item Description: | Literaturverz. S. 203 - 207. - Dt. Ausg. u.d.T.: Engelhardt, Heinz: Kapillarelektrophorese |
| Physical Description: | X, 215 S. graph. Darst. |
| ISBN: | 3528066687 |
Staff View
MARC
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| 001 | BV010483974 | ||
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| 007 | t | ||
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| 016 | 7 | |a 946006229 |2 DE-101 | |
| 020 | |a 3528066687 |c Pp. : DM 78.00 |9 3-528-06668-7 | ||
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| 035 | |a (DE-599)BVBBV010483974 | ||
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| 100 | 1 | |a Engelhardt, Heinz |e Verfasser |4 aut | |
| 245 | 1 | 0 | |a Capillary electrophoresis |b methods and potentials |c Heinz Engelhardt ; Wolfgang Beck ; Thomas Schmitt. Transl. from the German by George Gutnikov |
| 264 | 1 | |a Braunschweig ; Wiesbaden |b Vieweg |c 1996 | |
| 300 | |a X, 215 S. |b graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 500 | |a Literaturverz. S. 203 - 207. - Dt. Ausg. u.d.T.: Engelhardt, Heinz: Kapillarelektrophorese | ||
| 650 | 4 | |a Capillary electrophoresis | |
| 650 | 0 | 7 | |a Kapillarelektrophorese |0 (DE-588)4290002-5 |2 gnd |9 rswk-swf |
| 689 | 0 | 0 | |a Kapillarelektrophorese |0 (DE-588)4290002-5 |D s |
| 689 | 0 | |5 DE-604 | |
| 700 | 1 | |a Beck, Wolfgang |e Verfasser |4 aut | |
| 700 | 1 | |a Schmitt, Thomas |e Verfasser |4 aut | |
| 856 | 4 | 2 | |m DNB Datenaustausch |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=006986776&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
| 943 | 1 | |a oai:aleph.bib-bvb.de:BVB01-006986776 | |
Record in the Search Index
| _version_ | 1807773821282811904 |
|---|---|
| adam_text |
V
TABLE
OF
CONTENTS
PREFACE
.
IX
1
INTRODUCTION
.
1
2
PRINCIPLES
OF
CAPILLARY
ELECTROPHORESIS
.
3
3
THEORETICAL
FOUNDATIONS
AND
THEIR
INFLUENCE
ON
THE
ANALYTICAL
RESULTS
.
7
3.1
ELECTROPHORETIC
MIGRATION
.
7
3.2
CONDUCTIVITY
.
9
3.3
ELECTROOSMOTIC
FLOW
.
11
3.4
BAND
BROADENING
.
19
3.4.1
EFFICIENCY
LOSSES
THROUGH
DIFFUSION
.
20
3.4.2
EFFICIENCY
LOSS
THROUGH
TEMPERATURE
EFFECTS
.
23
3.4.3
LOSS
IN
EFFICIENCY
THROUGH
ELECTRODISPERSION
.
26
3.4.4
EFFICIENCY
LOSSES
THROUGH
WALL
ADSORPTION
.
29
3.4.5
EFFICIENCY
LOSSES
THROUGH
OVERLOADING
OF
THE
SEPARATION
SYSTEM
.
31
3.4.6
EFFICIENCY
LOSSES
THROUGH
SUPERIMPOSITION
OF
FLOW
PROFILES
.
32
3.4.7
SUMMARY
.
33
4
INSTRUMENTATION
.
34
4.1
POWER
SUPPLY
.
34
4.2
CAPILLARIES
.
34
4.3
SAMPLE
INTRODUCTION
.
38
4:3.1
PRESSURE
INJECTION
.
39
4.3.2
HYDROSTATIC
INJECTION
.
41
4.3.3
ELECTROKINETIC
INJECTION
.
42
4.3.4
SAMPLE-SPLIT
SYSTEMS
.
44
4.3.5
ENRICHMENT
EFFECTS
IN
SAMPLE
INTRODUCTION:
SAMPLE
STACKING
.
45
4.4
THERMOSTATING
.
49
4.5
DETECTION
.
50
4.5.1
UV
DETECTION
.
51
4.5.2
FLUORESCENCE
DETECTION
.
58
VI
4.5.3
CONDUCTIVITY
DETECTION
.
59
4.5.4
OTHER
DETECTION
METHODS
.
61
4.5.5
DERIVATIZATION
REACTIONS
.
61
4.6
SPECIAL
PROBLEMS
OF
QUANTITATIVE
ANALYSIS
IN
CE
.
66
5
CAPILLARY
ZONE
ELECTROPHORESIS
(CZE)
.
73
5.1
PRINCIPLES
OF
OPTIMIZATION
IN
CZE
.
75
5.1.1
EFFECT
OF
PH
.
75
5.1.2
EFFECT
OF
BUFFER
CONCENTRATION
.
76
5.1.3
BUFFER
SELECTION
.
77
5.1.4
APPLICATIONS
.
79
5.2
INDIRECT
DETECTION
METHODS
IN
CE
.
80
5.2.1
PRINCIPLES
OF
INDIRECT
DETECTION
TECHNIQUES
.
80
5.2.2
SEPARATION
OF
CATIONS
WITH
INDIRECT
UV
DETECTION
.
82
5.2.3
SEPARATION
OF
ANIONS
WITH
INDIRECT
UV
DETECTION
.
91
5.2.4
ANALYSIS
OF
CATIONS
AND
ANIONS
WITH
INDIRECT
FLUORESCENCE
DETECTION
.
100
5.3
CAPILLARY
ZONE
ELECTROPHORESIS
OF
PROTEINS
.
102
5.3.1
SEPARATIONS
IN
UNCOATED
CAPILLARIES
.
102
5.3.1.1
SELECTION
OF
THE
PH
.
102
5.3.1.2
ADDITION
OF
SALTS
TO
THE
BUFFER
.
104
5.3.1.3
USE
OF
BUFFER
ADDITIVES
FOR
THE
SEPARATION
OF
PROTEINS
.
105
5.3.1.4
DYNAMIC
COATING
OF
CAPILLARIES
.
107
5.3.2
PROTEIN
SEPARATIONS
WITH
SURFACE-MODIFIED
CAPILLARIES
.
107
5.3.2.1
COATINGS
FOR
CAPILLARY
ELECTROPHORESIS
.
108
5.3.3
OVERVIEW
OF
IMPORTANT
CHEMICAL
COATINGS
FOR
PROTEIN
SEPARATION
.
ILL
5.3.3.1
CONVENTIONAL
COATINGS
.
ILL
5.3.3.2
POLYMERIC
COATINGS
.
115
5.3.4
SUMMARY
.
120
6
MICELLAR
ELECTROKINETIC
CHROMATOGRAPHY
(MEKC)
123
6.1
FUNDAMENTALS
OF
MEKC
.
123
6.2
OPTIMIZATION
OF
RESOLUTION
.
126
6.3
SELECTION
OF
THE
DETERGENT
.
129
6.4
SEPARATIONS
BY
MEKC
.
131
VII
7
SEPARATION
OF
ENANTIOMERS
BY
CE
.
140
7.1
ENANTIOMERIC
SEPARATIONS
WITH
CYCLODEXTRINS
AS
CHIRAL
SELECTORS
.
145
7.1.1
NEUTRAL
CYCLODEXTRINS
.
149
7.1.2
IONIC
CYCLODEXTRINS
.
158
7.2
OTHER
SEPARATION
SYSTEMS
.
162
8
CAPILLARY
GEL
ELECTROPHORESIS
(CGE)
.
166
8.1
ACRYLAMIDE-BASED
GELS
.
167
8.1.1
PREPARATION
AND
MANIPULATION
OF
GEL-FILLED
CAPILLARIES
.
168
8.1.2
CROSSLINKED
POLYACRYLAMIDE
GELS
.
171
8.1.3
LINEAR
POLYACRYLAMIDE
GELS
(LPA)
.
172
8.1.3.1
SEPARATION
OF
DNA
FRAGMENTS
WITH
LPA
.
174
8.1.3.2
SDS
PAGE
(POLYACRYLAMIDE
GEL
ELECTROPHORESIS)
OF
PROTEINS
.
177
8.2
POLYSACCHARIDE-BASED
GELS
AND
OTHER
POLYMERS
.
178
8.3
MIGRATION MODELS
OF
BIOPOLYMERS
IN
POLYMER
SOLUTIONS
.
180
9
ISOELECTRIC
FOCUSING
IN
CAPILLARIES
(CIEF)
.
186
10
OTHER
SEPARATION
TECHNIQUES
IN
CE
.
192
10.1
ISOTACHOPHORESIS
(ITP)
.
192
10.2
ELECTROCHROMATOGRAPHY
(EC)
.
194
11
A
TROUBLESHOOTING
GUIDE
TO
CE
.
196
11.1
DETERMINATION
OF
THE
PROBLEM
SOURCE
.
196
TEST
1:
RECORDING
A
BREAKTHROUGH
CURVE
UNDER
FLUSHING
PRESSURE
.
197
TEST
2:
RECORDING
A
BREAKTHROUGH
CURVE
WITH
THE
INJECTION
PRESSURE
.
197
TEST
3:
CHECKING
THE
POTENTAL
SOURCE
.
197
11.2
SCENARIOS
OF
PROBLEMS:
'
'
WHAT
TO
DO
IF
.
"
.
198
12
LITERATURE
INDEX
.
203
12.1
LITERATURE
CITED
.
203
12.2
ADDITIONAL
LITERATURE
SOURCES
.
206
13
ACKNOWLEDGEMENT
.
208
SUBJECT
INDEX
209 |
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| dewey-ones | 543 - Analytical chemistry |
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| dewey-search | 543.0871 |
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| dewey-tens | 540 - Chemistry and allied sciences |
| discipline | Chemie / Pharmazie Chemie |
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| id | DE-604.BV010483974 |
| illustrated | Illustrated |
| indexdate | 2024-08-19T00:31:01Z |
| institution | BVB |
| isbn | 3528066687 |
| language | German |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-006986776 |
| oclc_num | 35677351 |
| open_access_boolean | |
| owner | DE-91 DE-BY-TUM DE-19 DE-BY-UBM DE-526 |
| owner_facet | DE-91 DE-BY-TUM DE-19 DE-BY-UBM DE-526 |
| physical | X, 215 S. graph. Darst. |
| publishDate | 1996 |
| publishDateSearch | 1996 |
| publishDateSort | 1996 |
| publisher | Vieweg |
| record_format | marc |
| spelling | Engelhardt, Heinz Verfasser aut Capillary electrophoresis methods and potentials Heinz Engelhardt ; Wolfgang Beck ; Thomas Schmitt. Transl. from the German by George Gutnikov Braunschweig ; Wiesbaden Vieweg 1996 X, 215 S. graph. Darst. txt rdacontent n rdamedia nc rdacarrier Literaturverz. S. 203 - 207. - Dt. Ausg. u.d.T.: Engelhardt, Heinz: Kapillarelektrophorese Capillary electrophoresis Kapillarelektrophorese (DE-588)4290002-5 gnd rswk-swf Kapillarelektrophorese (DE-588)4290002-5 s DE-604 Beck, Wolfgang Verfasser aut Schmitt, Thomas Verfasser aut DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=006986776&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Engelhardt, Heinz Beck, Wolfgang Schmitt, Thomas Capillary electrophoresis methods and potentials Capillary electrophoresis Kapillarelektrophorese (DE-588)4290002-5 gnd |
| subject_GND | (DE-588)4290002-5 |
| title | Capillary electrophoresis methods and potentials |
| title_auth | Capillary electrophoresis methods and potentials |
| title_exact_search | Capillary electrophoresis methods and potentials |
| title_full | Capillary electrophoresis methods and potentials Heinz Engelhardt ; Wolfgang Beck ; Thomas Schmitt. Transl. from the German by George Gutnikov |
| title_fullStr | Capillary electrophoresis methods and potentials Heinz Engelhardt ; Wolfgang Beck ; Thomas Schmitt. Transl. from the German by George Gutnikov |
| title_full_unstemmed | Capillary electrophoresis methods and potentials Heinz Engelhardt ; Wolfgang Beck ; Thomas Schmitt. Transl. from the German by George Gutnikov |
| title_short | Capillary electrophoresis |
| title_sort | capillary electrophoresis methods and potentials |
| title_sub | methods and potentials |
| topic | Capillary electrophoresis Kapillarelektrophorese (DE-588)4290002-5 gnd |
| topic_facet | Capillary electrophoresis Kapillarelektrophorese |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=006986776&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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