DNA fingerprinting in plants and fungi:
Saved in:
| New Title: | DNA fingerprinting in plants |
|---|---|
| Format: | Book |
| Language: | English |
| Published: |
Boca Raton u.a.
CRC Press
1995
|
| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Item Description: | 2. Aufl. u.d.T.: DNA fingerprinting in plants |
| Physical Description: | 322 S. Ill., graph. Darst. |
| ISBN: | 0849389208 |
Staff View
MARC
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| 245 | 1 | 0 | |a DNA fingerprinting in plants and fungi |c Kurt Weising ... |
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Record in the Search Index
| _version_ | 1804124749670907904 |
|---|---|
| adam_text | V) DNA FINGERPRINTING IN PLFLRFMDIM KURT WEISING * HILDE NYBOM KIRSTEN
WOLFF * WIELAND MEYER CRC PRESS BOCA RATON ANN ARBOR LONDON TOKYO TABLE
OF CONTENTS CHAPTER 1 INTRODUCTION 1 CHAPTER 2 GENETIC VARIATION AT THE
DNA LEVEL 3 I. A SHORT SURVEY OF MOLECULAR MARKERS 3 A. PROPERTIES OF
MOLECULAR MARKERS 3 B. PROTEIN MARKERS 3 C. DNA SEQUENCING 4 D.
RESTRICTION FRAGMENT LENGTH POLYMORPHISMS (RFLPS) 5 1. RFLPS IN NUCLEAR
DNA 5 2. RFLPS IN CYTOPLASMIC DNA: CPDNA AND MTDNA 7 E. DNA
FINGERPRINTING BASED ON HYBRIDIZATION 8 F. MOLECULAR MARKERS BASED ON
DNA AMPLIFICATION 9 G. MISCELLANEOUS MARKERS 10 II. MINISATELLITES AND
SIMPLE SEQUENCES: THE MOLECULAR BASIS OF CLASSICAL DNA FINGERPRINTING 10
A. THE NOMENCLATURE OF TANDEM-REPEATED DNA ELEMENTS 10 B. DNA
FINGERPRINTING WITH TANDEM-REPETITIVE PROBES 12 1. HUMAN MINISATELLITES
12 2. OTHER MINISATELLITES 13 3. SIMPLE SEQUENCE PROBES 13 4. FACTORS
AFFECTING THE LEVEL OF DNA FINGERPRINT POLYMORPHISM 17 C. CHROMOSOMAL
LOCALIZATION OF MINISATELLITES AND SIMPLE SEQUENCES 17 D. MECHANISMS
RESPONSIBLE FOR MINISATELLITE AND SIMPLE SEQUENCE POLYMORPHISM ; 20 E.
FUNCTIONAL SIGNIFICANCE OF MINISATELLITES AND SIMPLE SEQUENCES 21 1.
TELOMERES 21 2. CENTROMERES 22 3. TRANSCRIPTION 22 4. TRANSCRIPTIONAL
REGULATION ;.....22 5. RECOMBINATION 23 6. REPLICATION 23 7. GENERAL
CONSIDERATIONS 23 III. DETECTION OF DNA POLYMORPHISM BY PCR-BASED
FINGERPRINTING 24 A. PRINCIPLE OF THE PCR ..24 B. PCR WITH MINISATELLITE
AND SIMPLE SEQUENCE PRIMERS 27 C. PCR WITH ARBITRARY PRIMERS 28 CHAPTER
3 LABORATORY EQUIPMENT 37 CHAPTER 4 METHODOLOGY 43 I. SAFETY PRECAUTIONS
43 II. DNA ISOLATION AND PURIFICATION 44 A. ISOLATION OF PLANT DNA 44 1.
COLLECTION AND PRESERVATION OF PLANT TISSUE 50 2. ISOLATION PROTOCOLS 51
A. CTAB PROCEDURE 51 B. DNA MINIPREPARATION 55 C. POTASSIUM ACETATE
PROCEDURE 55 D. DNA PREPARATION VIA NUCLEI 57 3. DNA PURIFICATION VIA
CSCL DENSITY GRADIENT CENTRIFUGATION 59 B. ISOLATION OF FUNGAL DNA 61 1.
ISOLATION AND CULTIVATION OF FUNGI FROM PLANT MATERIAL, SOIL, AND
AIRBORNE SPORES 62 2. ISOLATION AND CULTIVATION OF HUMAN AND ANIMAL
PATHOGENIC FUNGI 62 3. LONG-TERM MAINTENANCE OF FUNGAL CULTURES 63 A.
STORAGE IN STERILE WATER 63 B. STORAGE IN CULTURE TUBES (SLANTS) 63 C.
STORAGE AS FROZEN GLYCEROL STOCKS 64 4. INDUCTION OF SPORULATION IN
FUNGAL CULTURES 64 5. LARGE-SCALE ACCUMULATION OF FUNGAL MATERIAL FOR
DNA ISOLATION. 65 A. CULTURING OF FILAMENTOUS FUNGI IN LIQUID MEDIA. 66
B. CULTURING OF YEAST-LIKE FUNGI IN LIQUID MEDIA..; 67 6. DNA ISOLATION
PROTOCOLS 68 A. SDS PROCEDURES 69 B. TNS/PAS PROCEDURE ,....72 C. DNA
PREPARATION VIA PROTOPLASTS 73 C. QUANTITATION OF DNA 75 1. ETHIDIUM
BROMIDE STAINING 75 2. SPECTROPHOTOMETRY 76 III. DNA FINGERPRINTING
BASED ON HYBRIDIZATION 77 A. RESTRICTION OF DNA 77 1. CHOICE OF THE
ENZYME 77 2. REACTION COMPONENTS AND CONDITIONS 78 3. SETTING UP
REACTIONS 79 B. AGAROSE GEL ELECTROPHORESIS 79 C. GEL DRYING 82 D.
SOUTHERN TRANSFER 84 E. GENERATION OF PROBES 86 1. COMMERCIALLY
AVAILABLE PROBES 87 2. CHEMICAL SYNTHESIS OF OLIGONUCLEOTIDES 87 3.
ISOLATION OF FINGERPRINT PROBES FROM GENOMIC LIBRARIES 88 4. ISOLATION
AND PROCESSING OF THE M13 PROBE 89 A. PREPARATION OF COMPETENT CELLS 89
B. TRANSFECTION OF COMPETENT CELLS WITH ML3 PHAGE 90 C. AMPLIFICATION OF
M13 PHAGE 91 D. M13 DNA ISOLATION 91 E. ISOLATION OF A 780-BP M13
FRAGMENT 92 F. LABELING OF PROBES 93 1. END LABELING 94 2. NICK
TRANSLATION 95 3. RANDOM PRIMING 96 4. REMOVAL OF UNINCORPORATED DNTPS
98 A. CHROMATOGRAPHY ON SEPHADEX G-50 98 B. SPIN-COLUMN TECHNIQUE 99 5.
NONRADIOACTIVE LABELING PROCEDURES 100 G. HYBRIDIZATION 100 1.
HYBRIDIZATION WITH OLIGONUCLEOTIDE PROBES 101 A. GEL HYBRIDIZATION WITH
RADIOACTIVE PROBES 101 B. BLOT HYBRIDIZATION WITH NONRADIOACTIVE PROBES
102 2. HYBRIDIZATION WITH M13 AND OTHER MINISATELLITE PROBES 103 A.
STANDARD PROTOCOL 103 B. TWO-STEP PROTOCOL 104 3. STRIPPING OF
HYBRIDIZED GELS AND MEMBRANES 105 H. SIGNAL DETECTION 106 1.
AUTORADIOGRAPHY 106 2. PHOSPHORIMAGING 107 3. NONRADIOACTIVE ASSAYS 108
IV. PCR-BASED DNA FINGERPRINTING 112 A. PCR WITH ARBITRARY PRIMERS 112
1. STANDARD RAPD PROTOCOL 113 2. INFLUENCE OF REACTION CONDITIONS AND
COMPONENTS 114 A. PRIMERS 114 B. POLYMERASE 115 C. THERMOCYCLER 115 D.
TEMPERATURE PROFILE 116 E. TEMPLATE CONCENTRATION 117 F. MG 2+
CONCENTRATION 118 G. REPRODUCIBILITY AND QUALITY OF BANDING PATTERNS 118
H. MODIFICATIONS 120 3. RAPD FRAGMENTS USED AS PROBES 121 4. STANDARD
DAF PROTOCOL 123 5. STANDARD AP-PCR PROTOCOL 125 B. PCR WITH SIMPLE
SEQUENCE AND MINISATELLITE PRIMERS 126 C. SEPARATION AND DETECTION OF
PCR PRODUCTS ,129 1. SEPARATION PROCEDURES 129 A. AGAROSE GEL
ELECTROPHORESIS 129 B. POLYACRYLAMIDE GEL ELECTROPHORESIS 130 2.
DETECTION PROCEDURES 133 A. ETHIDIUM BROMIDE STAINING 133 B. SILVER
STAINING 135 V. EVALUATION OF FRAGMENT PATTERNS 136 A. FRAGMENT SIZING
AND MATCHING 136 B. STATISTICAL PROCEDURES 139 1. INTRODUCTION 139 2.
BAND SHARING, SIMILARITY INDEX, AND GENETIC DISTANCE 140 3. POPULATION
GENETIC ANALYSES 143 A. CALCULATION OF MUTATION RATE 143 B. POPULATION
SIZE 143 C. HETEROZYGOSITY 144 D. INBREEDING COEFFICIENT 144 E. GENETIC
DIVERSITY AND POPULATION SUBDIVISION 144 F. PATERNITY TESTING AND
ESTIMATION OF RELATEDNESS 146 G. OUTCROSSING RATES 148 4. PHENETIC AND
PHYLOGENETIC ANALYSIS 148 A. ORDINATION TECHNIQUES 149 B. CONSTRUCTION
OF DENDROGRAMS 149 C. ASSUMPTIONS AND LIMITATIONS 151 5. LINKAGE
ANALYSIS AND GENETIC MAPPING 152 CHAPTER 5 APPLICATIONS OF DNA
FINGERPRINTING IN PLANTS AND FUNGI ..157 I. DEVELOPMENT OF METHODS AND
INITIAL INVESTIGATIONS 157 A. MINISATELLITE DNA PROBES DETECT GENETIC
VARIATION 157 B. OLIGONUCLEOTIDE PROBES ARE INTRODUCED 158 C. PCR-BASED
METHODS PROMPT INCREASED RESEARCH ACTIVITY ;. 158 II. WILD PLANT SPECIES
159 A. DNA FINGERPRINTS DETECT GENETIC DIVERSITY AND RELATEDNESS IN
PLANT POPULATIONS 159 1. CROSS-POLLINATION VS. SELFING: THE INFLUENCE OF
THE BREEDING SYSTEM ON DIVERSITY 160 2. VEGETATIVE PROPAGATION,
SEXUALITY, AND APOMIXIS: THE INFLUENCE OF THE REPRODUCTIVE SYSTEM ON
DIVERSITY 164 3. RARE SPECIES 165 4. GYMNOSPERMS 165 5. GENERAL
CONSIDERATIONS 166 B. DNA FINGERPRINTS YIELD INSIGHTS INTO PLANT
TAXONOMY 167 1. APOMICTIC MICROSPECIES 167 2. SEXUAL SPECIES 169 3.
OTHER PLANT GROUPS 170 4. GENERAL CONSIDERATIONS 170 III. CULTIVATED
PLANT SPECIES 171 A. CULTIVAR IDENTIFICATION AND ESTIMATION OF GENETIC
RELATEDNESS 171 1. VEGETATIVELY PROPAGATED CULTIVARS 171 2.
SEED-PROPAGATED CULTIVARS 172 3. VEGETATIVE SPORTS AND OTHER MUTANTS 183
4. IN VIYRO-PROPAGATED PLANT MATERIAL AND SOMACLONAL VARIATION 184 5.
GENERAL CONSIDERATIONS 186 B. PATERNITY TESTING AND THE IDENTIFICATION
OF HYBRIDS 188 1. SEED-PROPAGATED PLANTS AND SEXUAL HYBRIDS 188 2.
SOMATIC HYBRIDS 190 3. GENERAL CONSIDERATIONS 191 IV. INHERITANCE OF DNA
FINGERPRINTS, LINKAGE ANALYSIS, AND GENOME MAPPING IN PLANTS 191 A.
MINISATELLITE AND SIMPLE SEQUENCE HYBRIDIZATION 192 B. PCR-BASED METHODS
192 C. GENERAL CONSIDERATIONS 200 V. FUNGI 201 A. HUMAN PATHOGENIC
FUNGI: DIAGNOSTICS AND EPIDEMIOLOGY 202 1. CANDIDA 203 2. CRYPTOCOCCUS
205 3. SPOROTHRIX .., 207 4. PNEUMOCYSTIS 207 5. ASPERGILLUS 207 6.
HISTOPLASMA 208 B. PLANT PATHOGENIC FUNGI: POPULATION GENETICS,
EPIDEMIOLOGY, AND PATHOTYPE IDENTIFICATION 209 1. ASCOCHYTA * . 209 2.
COLLETOTRICHUM 209 3. ERYSIPHE 211 4. FUSARIUM 212 5. LEPTOSPHAERIA 213
6. MAGNAPORTHE 215 7. OPHIOSTOMA 216 8. PHYTOPHTHORA 216 9. SCLEROTINIA
217 C. INSECT-PATHOGENIC FUNGI 218 D. NONPATHOGENIC FUNGI 218 E.
COMMERCIALLY IMPORTANT FUNGI 219 1. YEASTS 219 2. MUSHROOMS 220 F.
TAXONOMY AND PHYLOGENY 222 G. INHERITANCE OF DNA FINGERPRINTS, LINKAGE
ANALYSIS, AND GENETIC MAPPING 224 H. GENERAL CONSIDERATIONS 225 VI.
CONCLUSIONS 226 CHAPTER 6 COMPARISON OF METHODS FOR DETECTING GENETIC
VARIATION 229 I. MORPHOLOGICAL CHARACTERS AND ALLOZYMES VS. DNA MARKERS
229 II. DIFFERENT KINDS OF MOLECULAR MARKERS 230 A. DISCRIMINATORY POWER
AND CONSISTENCY 230 B. COSTS 232 C. CONCLUSIONS 233 CHAPTER 7 FUTURE
PROSPECTS 235 I. IMPROVEMENT OF EXISTING METHODS 235 II. EXTENDING THE
SPECTRUM OF DNA TYPING STRATEGIES 237 A. POLYMORPHIC SINGLE-LOCUS
HYBRIDIZATION PROBES 238 B. PCR-AMPLIFIED MICROSATELLITES 239 C.
SEQUENCE CHARACTERIZED AMPLIFIED REGIONS (SCARS) 241 D. MISCELLANEOUS
TECHNIQUES 242 E. OUTLOOK 244 REFERENCES .*...:. .245 APPENDICES 287
INDEX 315
|
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| callnumber-label | QK981 |
| callnumber-raw | QK981.45 |
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| callnumber-sort | QK 3981.45 |
| callnumber-subject | QK - Botany |
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| dewey-ones | 581 - Specific topics in natural history of plants |
| dewey-raw | 581.87/3282 |
| dewey-search | 581.87/3282 |
| dewey-sort | 3581.87 43282 |
| dewey-tens | 580 - Plants |
| discipline | Biologie Agrarwissenschaft Pflanzenbau |
| format | Book |
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| id | DE-604.BV010329307 |
| illustrated | Illustrated |
| indexdate | 2024-07-09T17:50:37Z |
| institution | BVB |
| isbn | 0849389208 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-006874272 |
| oclc_num | 31377103 |
| open_access_boolean | |
| owner | DE-355 DE-BY-UBR DE-M49 DE-BY-TUM DE-703 DE-83 DE-11 DE-188 |
| owner_facet | DE-355 DE-BY-UBR DE-M49 DE-BY-TUM DE-703 DE-83 DE-11 DE-188 |
| physical | 322 S. Ill., graph. Darst. |
| publishDate | 1995 |
| publishDateSearch | 1995 |
| publishDateSort | 1995 |
| publisher | CRC Press |
| record_format | marc |
| spelling | DNA fingerprinting in plants and fungi Kurt Weising ... Plants and fungi Boca Raton u.a. CRC Press 1995 322 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier 2. Aufl. u.d.T.: DNA fingerprinting in plants Champignons - Empreintes génétiques DNA-vingerafdrukken gtt Fungi (Eumycophyta) larpcal Fungi gtt Genetica Vegetal larpcal Nucleo (Citologia) larpcal Planten gtt Plantes - Empreintes génétiques DNA Fingerprinting DNA fingerprinting of fungi DNA fingerprinting of plants Fungi genetics Plants genetics Pflanzen (DE-588)4045539-7 gnd rswk-swf Pilze (DE-588)4046076-9 gnd rswk-swf Fingerprint-Verfahren (DE-588)4231579-7 gnd rswk-swf Genetischer Fingerabdruck (DE-588)4245149-8 gnd rswk-swf Pflanzen (DE-588)4045539-7 s Genetischer Fingerabdruck (DE-588)4245149-8 s DE-604 Fingerprint-Verfahren (DE-588)4231579-7 s DE-188 Pilze (DE-588)4046076-9 s Weising, Kurt Sonstige oth 2. Aufl. u.d.T. DNA fingerprinting in plants (DE-604)BV019414749 GBV Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=006874272&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | DNA fingerprinting in plants and fungi Champignons - Empreintes génétiques DNA-vingerafdrukken gtt Fungi (Eumycophyta) larpcal Fungi gtt Genetica Vegetal larpcal Nucleo (Citologia) larpcal Planten gtt Plantes - Empreintes génétiques DNA Fingerprinting DNA fingerprinting of fungi DNA fingerprinting of plants Fungi genetics Plants genetics Pflanzen (DE-588)4045539-7 gnd Pilze (DE-588)4046076-9 gnd Fingerprint-Verfahren (DE-588)4231579-7 gnd Genetischer Fingerabdruck (DE-588)4245149-8 gnd |
| subject_GND | (DE-588)4045539-7 (DE-588)4046076-9 (DE-588)4231579-7 (DE-588)4245149-8 |
| title | DNA fingerprinting in plants and fungi |
| title_alt | Plants and fungi |
| title_auth | DNA fingerprinting in plants and fungi |
| title_exact_search | DNA fingerprinting in plants and fungi |
| title_full | DNA fingerprinting in plants and fungi Kurt Weising ... |
| title_fullStr | DNA fingerprinting in plants and fungi Kurt Weising ... |
| title_full_unstemmed | DNA fingerprinting in plants and fungi Kurt Weising ... |
| title_new | DNA fingerprinting in plants |
| title_short | DNA fingerprinting in plants and fungi |
| title_sort | dna fingerprinting in plants and fungi |
| topic | Champignons - Empreintes génétiques DNA-vingerafdrukken gtt Fungi (Eumycophyta) larpcal Fungi gtt Genetica Vegetal larpcal Nucleo (Citologia) larpcal Planten gtt Plantes - Empreintes génétiques DNA Fingerprinting DNA fingerprinting of fungi DNA fingerprinting of plants Fungi genetics Plants genetics Pflanzen (DE-588)4045539-7 gnd Pilze (DE-588)4046076-9 gnd Fingerprint-Verfahren (DE-588)4231579-7 gnd Genetischer Fingerabdruck (DE-588)4245149-8 gnd |
| topic_facet | Champignons - Empreintes génétiques DNA-vingerafdrukken Fungi (Eumycophyta) Fungi Genetica Vegetal Nucleo (Citologia) Planten Plantes - Empreintes génétiques DNA Fingerprinting DNA fingerprinting of fungi DNA fingerprinting of plants Fungi genetics Plants genetics Pflanzen Pilze Fingerprint-Verfahren Genetischer Fingerabdruck |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=006874272&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT weisingkurt dnafingerprintinginplantsandfungi AT weisingkurt plantsandfungi |