Plant cell, tissue and organ culture: fundamental methods
Gespeichert in:
Format: | Buch |
---|---|
Sprache: | English |
Veröffentlicht: |
Berlin [u.a.]
Springer
1995
|
Schriftenreihe: | Springer lab manual
|
Schlagworte: | |
Online-Zugang: | Inhaltsverzeichnis |
Beschreibung: | Hier auch später erschienene, unveränderte Nachdrucke |
Beschreibung: | XXIV, 358 S. Ill., graph. Darst. |
ISBN: | 3540580689 9783642489747 |
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245 | 1 | 0 | |a Plant cell, tissue and organ culture |b fundamental methods |c O. L. Gamborg ... (ed.) |
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Datensatz im Suchindex
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adam_text | O. L. GAMBORG G. C. PHILLIPS (EDS.)
PLANT CELL,
TISSUE AND ORGAN
CULTURE
FUNDAMENTA
L METHOD
S
WITH 86 FIGURES
SPRINGER
CONTENTS
SECTION 1 FACILITIES AND TECHNOLOGY REQUIREMENTS
FOR LABORATORY OPERATIONS
CHAPTER 1 LABORATORY FACILITIES, OPERATION, AND MANAGEMENT
O.L.
GAMBORG
AND G.C.
PHILLIP
S
(WITH 2 FIGURES) 3
1.1 INTRODUCTION 3
1.2 OBJECTIVES AND GOALS 3
1.3 LABORATORY DESIGN AN
D DEVELOPMENT 4
1.3.1 GENERAL LABORATORY AN
D MEDIA PREPARATIO
N 4
1.3.2 STERILE TRANSFER ROOM 5
1.3.3 CULTURING FACILITY 7
1.3.4 CLEANING ROOM 7
1.3.5 INSTRUMENT ROOM 7
1.3.6 GREENHOUSES 8
1.3.7 SCALE-UP AND SPECIAL NEEDS 8
1.4 EQUIPMENT AN
D APPARATU
S 8
1.4.1 MEDIA PREPARATION AN
D STERILIZATION AREA 8
1.4.2 STERILE TRANSFER ROOM 9
1.4.3 CULTURING FACILITIES 9
1.5 LABORATORY MANAGEMENT 10
1.5.1 LABORATORY MANAGER OR COORDINATO
R 10
1.5.2 SCHEDULE ASSIGNMENTS 10
1.5.3 FACILITY MANAGEMENT 10
1.5.4 OPERATION MANAGEMENT 13
1.5.5 SETTING OBJECTIVES AN
D GOALS 15
1.6 LABORATORY AND PERSONAL SAFETY 16
1.7 EMERGENCY SYSTEMS 17
1.8 DEFINITIONS OF LIGHT AN
D ENERGY UNITS 18
1.9 SOME USEFUL REFERENCE BOOKS 18
1.10 STUDY QUESTIONS 19
REFERENCES 19
XIV CONTENTS
6.4.2 TREATMENT OF MATERIALS 70
6.4.3 DESIGN OF EXPERIMENT 70
6.4.4 PROTOCOLS 70
6.4.5 SCHEDULE OF OBSERVATIONS AN
D MEASUREMENTS 74
6.5 RESULTS V 74
6.6 PROBLEM SOLVING 75
6.7 STUDY QUESTIONS 78
REFERENCES AN
D ADVANCED READINGS 78
CHAPTER 7 PLANT REGENERATION FROM CALLUS AND CELL SUSPENSION CULTURES
BY SOMATIC EMBRYOGENESIS
G.C.
PHILLIPS
,
J.F.
HUBSTENBERGER
,
AND E.E.
HANSE
N
(WITH 4 FIGURES) 81
7.1 INTRODUCTIO
N 81
7.2 OBJECTIVES AN
D GOALS 81
7.3 EQUIPMENT, FACILITIES, AN
D MATERIALS 82
7.3.1 PLANT MATERIALS 82
7.3.2 EQUIPMENT 82
7.3.3 MEDIA 82
7.4 PROCEDURES 82
7.4.1 PREPARATIO
N OF MEDIA 82
7.4.2 TREATMEN
T OF MATERIALS 83
7.4.3 DESIGN OF EXPERIMENT 83
7.4.4 PROTOCOLS 83
7.4.5 SCHEDULE OF OBSERVATIONS AN
D MEASUREMENTS , 86
7.5 RESULTS 87
7.6 PROBLEM SOLVING 87
7.7 STUDY QUESTIONS 90
REFERENCES AN
D ADVANCED READINGS 90
CHAPTER 8 DIRECT SOMATIC EMBRYOGENESIS
JJ.
FINE
R
(WITH 1 FIGURE) 91
8.1 INTRODUCTIO
N 91
8.2 OBJECTIVES AN
D GOALS ,. 92
8.3 EQUIPMENT, FACILITIES, AN
D MATERIALS 92
8.3.1 PLANT MATERIALS 92
8.3.2 EQUIPMENT 93
8.3.3 MEDIA AN
D REAGENTS 93
8.4 PROCEDURES 93
8.4.1 PREPARATIO
N OF REAGENTS AN
D MEDIA 93
8.4.2 TREATMEN
T OF MATERIALS 94
8.4.3 PURPOSE OF EXPERIMENTS 94
8.4.4 PROTOCOLS 94
CONTENTS XV
8.4.5 SCHEDULE OF OBSERVATIONS AN
D MEASUREMENTS 96
8.5 RESULTS 97
8.6 PROBLEM SOLVING 99
8.7 STUDY QUESTIONS 101
REFERENCES AND ADVANCED READINGS ; 101
CHAPTER 9 CONIFER SOMATIC EMBRYOGENESIS, EMBRYO DEVELOPMENT,
MATURATION DRYING AND PLANT FORMATION
S.M.
ATTRE
E
AND L.C.
FOWKE
(WITH 6 FIGURES) 103
9.1 INTRODUCTION 103
9.2 OBJECTIVES AND GOALS 104
9.3 EQUIPMENT, MATERIALS, AN
D REAGENTS 104
9.3.1 EQUIPMENT AN
D SUPPLIES 104
9.3.2 MEDIA 106
9.4 PROCEDURES 106
9.5 RESULTS 109
9.6 PROBLEM SOLVING IL
L
9.7 STUDY QUESTIONS 113
REFERENCES AND ADVANCED READINGS 113
SECTION 3 APPLICATIONS OF ORGAN CULTURE SYSTEMS
CHAPTER 10 MERISTEM CULTURE FOR VIRUS ELIMINATION
S.A.
SLACK
AND L.A.
TUFFOR
D
(WITH 4 FIGURES) 117
10.1 INTRODUCTION 117
10.2 OBJECTIVES AND GOALS 117
10.3 EQUIPMENT AN
D REAGENTS 118
10.4 PROCEDURES 118
10.4.1 PREPARATION OF MEDIA 118
10.4.2 TREATMENT OF MATERIALS 119
10.4.3 PROTOCOLS 120
10.5 RESULTS 124
10.6 PROBLEM SOLVING 127
10.7 STUDY QUESTIONS 127
REFERENCES AND ADVANCED READINGS 128
CHAPTER 11 EMBRYO CULTURE AND EMBRYO RESCUE
FOR WIDE CROSS HYBRIDS
C.Y. HU AND M.H.B.
ZANETTIN
I
(WITH 3 FIGURES) 129
11.1 INTRODUCTION 129
11.2 OBJECTIVES AND GOALS 130
XVI CONTENTS
11.3 EQUIPMENT AN
D MATERIALS 130
11.3.1 PLANT MATERIALS 130
11.3.2 FACILITIES, EQUIPMENT
, AN
D SUPPLIES 131
11.3.3 MEDIA AND REAGENTS 131
11.4 PROCEDURES . 132
11.4.1 TREATMEN
T OF MATERIALS 132
11.4.2 DISSECTION 132
11.4.3 PROTOCOLS 132
11.4.4 SCHEDULE OF OBSERVATIONS AND MEASUREMENTS 136
11.5 RESULTS 137
11.6 PROBLEM SOLVING 138
11.7 STUDY QUESTIONS 140
REFERENCES AN
D ADVANCED READINGS 140
CHAPTER 12 ANTHER CULTURE FOR DOUBLED HAPLOID PRODUCTION
T.P.
CROUGHA
N
(WITH 7 FIGURES) 143
12.1 INTRODUCTIO
N 143
12.2 OBJECTIVES AND GOALS 144
12.3 EQUIPMENT AN
D MATERIALS 145
12.3.1 PLANT MATERIALS 145
12.3.2 EQUIPMENT AN
D SUPPLIES 145
12.3.3 MEDIA AN
D REAGENTS 145
12.4 PROCEDURES 146
12.4.1 DESIGN OF EXPERIMENTS 146
12.4.2 TREATMEN
T OF DONO
R MATERIALS 146
12.4.3 PROTOCOLS 146
12.4.4 SCHEDULE OF OBSERVATIONS AN
D MEASUREMENTS 150
12.5 RESULTS 151
12.6 PROBLEM SOLVING . 153
12.7 STUDY QUESTIONS 153
REFERENCES AN
D ADVANCED READINGS 154
CHAPTER 13 MICROSPORE CULTURE FOR HAPLOID PLANT PRODUCTION
A.M.R.
FERRI
E
AN
D W.A.
KELLER
155
13.1 INTRODUCTIO
N 155
13.2 OBJECTIVES AN
D GOALS 156
13.3 EQUIPMENT, FACILITIES, AN
D MATERIALS 156
13.3.1 EQUIPMENT AN
D SUPPLIES 156
13.3.2 MEDIA AN
D REAGENTS 157
13.4 PROCEDURE
S 158
13.4.1 TREATMEN
T OF DONO
R MATERIALS 158
13.4.2 MICROSPORE STAINING 159
CONTENTS XVII
13.4.3 PROTOCOLS 159
13.4.4 SCHEDULE OF OBSERVATIONS AND MEASUREMENTS 161
13.5 RESULTS 161
13.6 PROBLEM SOLVING 161
13.7 STUDY QUESTIONS 163
REFERENCES AND ADVANCED READINGS YY ; 163
SECTION 4 PROTOPLASTS AND TRANSFORMATION PROCEDURES
CHAPTER 14 PROTOPLAST ISOLATION AND CULTURE
S.C.
GLEDDIE
(WITH 5 FIGURES) 167
14.1 INTRODUCTION 167
14.2 OBJECTIVES AN
D GOALS 168
14.3 EQUIPMENT, FACILITIES, AN
D MATERIALS 168
14.3.1 EQUIPMENT AN
D SUPPLIES 168
14.3.2 MEDIA 169
14.4 PROCEDURES 171
14.4.1 ENZYME STOCK SOLUTIONS 171
14.4.2 DONOR PLANT MATERIALS 171
14.4.3 PREPARATION OF EXPLANTS 172
14.4.4 PROTOCOLS 173
14.5 RESULTS 177
14.6 PROBLEM SOLVING 179
14.7 STUDY QUESTIONS 180
REFERENCES AND ADVANCED READINGS 180
CHAPTER 15 -AGROFOACTERIUM-MEDIATED TRANSFORMATION
E.
FIROOZABADY
AN
D A.R.
KUEHNL
E
(WITH 9 FIGURES) 181
15.1 INTRODUCTION 181
15.2 OBJECTIVES AN
D GOALS 183
15.3 EQUIPMENT, FACILITIES, AN
D MATERIALS 183
15.3.1 BIOLOGICAL MATERIALS 183
15.3.2 EQUIPMENT AN
D SUPPLIES 183
15.3.3 MEDIA 184
15.4 PROCEDURES 185
15.4.1 PREPARATION OF MEDIA AN
D REAGENTS 185
15.4.2 TREATMENT OF MATERIALS 186
15.4.3 PROTOCOLS 186
15.4.4 CONFIRMATION OF STABLE TRANSFORMATIONS 189
15.5 RESULTS .
. 189
15.6 PROBLEM SOLVING 193
15.7 STUDY QUESTIONS 194
REFERENCES AND ADVANCED READINGS 194
XVIII CONTENTS
CHAPTER 16 DIRECT DNA TRANSFER INTO INTACT PLANT CELLS
VIA MICROPROJECTILE BOMBARDMENT
D.T.
TOMES
,
M.C.
ROSS
,
AN
D D.D.
SONGSTAD
(WITH 2 FIGURES) 197
16.1 INTRODUCTIO
N 197
16.2 OBJECTIVES AN
D GOALS 197
16.3 EQUIPMENT, FACILITIES, AND MATERIALS 199
16.3.1 BIOLOGICAL MATERIALS 199
16.3.2 EQUIPMENT AN
D SUPPLIES 199
16.3.3 MEDIA 200
16.4 PROCEDURES 201
16.4.1 PREPARATIO
N OF REAGENTS AND SOLUTIONS 201
16.4.2 TREATMEN
T OF MATERIALS 202
16.4.3 DESIGN OF EXPERIMENTS 203
16.4.4 PROTOCOLS 203
16.5 RESULTS 208
16.6 PROBLEM SOLVING 212
16.7 STUDY QUESTIONS 213
REFERENCES AN
D ADVANCED READINGS 213
SECTION
5
MICROTECHNIQUES AND ANALYTICAL PROCEDURES
CHAPTER 17 BOTANICAL MICROTECHNIQUE FOR PLANT CULTURES
L.C.
FOWKE
AN
D P.J.
RENNI
E
(WITH 2 FIGURES) 217
17.1 INTRODUCTIO
N 217
17.2 OBJECTIVES AN
D GOALS 218
17.3 FAA FIXATION AN
D PARAFFIN EMBEDDING 218
17.3.1 EQUIPMENT
, MATERIALS, AN
D REAGENTS 218
17.3.2 PROCEDURES 218
17.3.3 RESULTS 221
17.3.4 PROBLEM SOLVING 223
17.4 GLUTARALDEHYDE FIXATION AN
D GMA EMBEDDING 223
17.4.1 EQUIPMENT, MATERIALS, AN
D REAGENTS 223
17.4.2 PROCEDURES 224
17.4.3 RESULTS 227
17.4.4 PROBLEM SOLVING 227
17.5 STUDY QUESTIONS 227
REFERENCES AN
D ADVANCED READINGS 228
CHAPTER 18 TRANSMISSION AND SCANNING ELECTRON MICROSCOPY
FOR PLANT PROTOPLASTS, CULTURED CELLS, AND TISSUES
L.C.
FOWKE
(WITH 3 FIGURES) 229
18.1 INTRODUCTIO
N 229
18.2 OBJECTIVES AN
D GOALS 230
CONTENTS XIX
18.3 TRANSMISSION ELECTRON MICROSCOPY 230
18.3.1 EQUIPMENT, MATERIALS, AN
D REAGENTS 230
18.3.2 PROCEDURES 231
18.3.3 RESULTS 233
18.3.4 PROBLEM SOLVING 235
18.4 SCANNING ELECTRON MICROSCOPY ^ 235
18.4.1 EQUIPMENT, MATERIALS, AN
D REAGENTS 235
18.4.2 PROCEDURES 235
18.4.3 RESULTS 236
18.4.4 PROBLEM SOLVING 237
18.5 STUDY QUESTIONS 238
REFERENCES AND ADVANCED READINGS 238
CHAPTER 19 INDIRECT IMMUNOFLUORESCENCE TECHNIQUES
FOR CULTURED PLANT CELLS AND PROTOPLASTS
H.
WAN
G
AN
D L.C.
FOWKE
(WITH 11 FIGURES) 239
19.1 INTRODUCTION 239
19.2 OBJECTIVES AN
D GOALS 240
19.3 EQUIPMENT, MATERIALS, AND REAGENTS 240
19.4 PROCEDURES 241
19.4.1 PREPARATION OF SOLUTIONS AN
D MATERIALS 241
19.4.2 PLANT CELLS AN
D PROTOPLASTS 242
19.4.3 PROTOCOLS 243
19.5 RESULTS 245
19.6 PROBLEM SOLVING 247
19.7 STUDY QUESTIONS 249
REFERENCES AND ADVANCED READINGS 249
CHAPTER 20 STAINING PROCEDURES FOR CHROMOSOME ANALYSIS
K.C.
ARMSTRONG
(WITH 3 FIGURES) 251
20.1 INTRODUCTION 251
20.2 OBJECTIVES AND GOALS 251
20.3 EQUIPMENT AND REAGENTS 251
20.4 PROCEDURES 253
20.4.1 PREPARATION OF STAINS 253
20.4.2 COLLECTION AND PREPARATIO
N OF PLANT MATERIALS 255
20.4.3 STAINING SCHEDULES 258
20.5 RESULTS 263
20.5.1 CHROMOSOME COUNTS -. 263
20.5.2 CHROMOSOME KARYOTYPES 263
20.5.3 MEIOTIC ANALYSIS 264
20.6 STUDY QUESTIONS 265
REFERENCES AND ADVANCED READINGS 265
XX CONTENTS
CHAPTER 21 ENZYME-LINKED IMMUNOSORBENT ASSAY (ELISA)
METHODS TO CERTIFY PATHOGEN (VIRUS)-FREE PLANTS
A.
ROWHAN
I
AN
D B.WI
FALK
(WITH 2 FIGURES) 267
21.1 INTRODUCTIO
N 267
21.2 OBJECTIVES AN
D GOALS
.. ..
269
21.3 EQUIPMENT AND REAGENTS 269
21.3.1 ANTISERA AN
D ANTIGENS 269
21.3.2 EQUIPMENT 269
21.4 PROCEDURE
S 270
21.4.1 PREPARATIO
N OF BUFFERS 270
21.4.2 SAMPLE PREPARATIO
N 272
21.4.3 PREPARATIO
N OF SEROLOGICAL REAGENTS 272
21.4.4 PROTOCOLS 275
21.5 RESULTS 277
21.6 PROBLEM SOLVING 279
21.7 STUDY QUESTIONS 279
REFERENCES AN
D ADVANCED READINGS 280
CHAPTER 22 APPLICATIONS OF POLYMERASE CHAIN REACTION (PCR)
TO PLANT GENOME ANALYSIS
M.J.
FOOLAD
,
S.
ARULSEKAR
,
AN
D R.L.
RODRIGUE
Z
(WITH 2 FIGURES)...
. 281
22.1 INTRODUCTIO
N 281
22.2 OBJECTIVES AND GOALS 282
22.3 EQUIPMENT, MATERIALS, AN
D REAGENTS 282
22.4 PROCEDURES 284
22.4.1 PREPARATIO
N OF MATERIALS AND REAGENTS 284
22.4.2 PROTOCOLS 286
22.5 APPLICATIONS 291
22.6 PROBLEM SOLVING 294
22.7 STUDY QUESTIONS 296
REFERENCES AN
D ADVANCED READINGS 296
APPENDICES
APPENDIX A: BASAL MEDIA FOR PLANT CELL AND TISSUE CULTURE
O.L.
GAMBORG
AND G.C.
PHILLIP
S
301
APPENDIX B: LISTS OF COMPOUNDS AND MOLECULAR WEIGHTS
O.L.
GAMBORG
309
APPENDIX C: ANSWERS TO STUDY QUESTIONS
(COMPLIED BY THE AUTHOR
S AN
D THE EDITORS) 315
CONTENTS XXI
APPENDIX D: LIST OF SUPPLIERS
(COMPILED BY THE AUTHOR
S AND THE EDITORS) 327
APPENDIX E: GLOSSARY OF TERMS USED IN PLANT CELLULAR
AND DEVELOPMENTAL BIOLOGY
G.C.
PHILLIP
S
AND O.L.
GAMBORG
337
SUBJECT INDEX
349
|
any_adam_object | 1 |
author_GND | (DE-588)172484707 |
building | Verbundindex |
bvnumber | BV010200703 |
classification_rvk | WN 5200 |
classification_tum | BIO 355f CIT 940f BIO 465f LAN 182f |
ctrlnum | (OCoLC)263569868 (DE-599)BVBBV010200703 |
dewey-full | 581/.0724 |
dewey-hundreds | 500 - Natural sciences and mathematics |
dewey-ones | 581 - Specific topics in natural history of plants |
dewey-raw | 581/.0724 |
dewey-search | 581/.0724 |
dewey-sort | 3581 3724 |
dewey-tens | 580 - Plants |
discipline | Biologie Agrarwissenschaft Chemie-Ingenieurwesen Biotechnologie Pflanzenbau |
format | Book |
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id | DE-604.BV010200703 |
illustrated | Illustrated |
indexdate | 2024-07-09T17:48:21Z |
institution | BVB |
isbn | 3540580689 9783642489747 |
language | English |
oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-006779032 |
oclc_num | 263569868 |
open_access_boolean | |
owner | DE-355 DE-BY-UBR DE-M49 DE-BY-TUM DE-703 DE-1028 DE-91G DE-BY-TUM DE-20 DE-188 DE-29T |
owner_facet | DE-355 DE-BY-UBR DE-M49 DE-BY-TUM DE-703 DE-1028 DE-91G DE-BY-TUM DE-20 DE-188 DE-29T |
physical | XXIV, 358 S. Ill., graph. Darst. |
publishDate | 1995 |
publishDateSearch | 1995 |
publishDateSort | 1995 |
publisher | Springer |
record_format | marc |
series2 | Springer lab manual |
spelling | Plant cell, tissue and organ culture fundamental methods O. L. Gamborg ... (ed.) Berlin [u.a.] Springer 1995 XXIV, 358 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Springer lab manual Hier auch später erschienene, unveränderte Nachdrucke Zellkultur (DE-588)4067547-6 gnd rswk-swf Pflanzen (DE-588)4045539-7 gnd rswk-swf Pflanzenzelle (DE-588)4115551-8 gnd rswk-swf Gewebekultur (DE-588)4157245-2 gnd rswk-swf In-vitro-Kultur (DE-588)4332883-0 gnd rswk-swf Pflanzenzelle (DE-588)4115551-8 s Zellkultur (DE-588)4067547-6 s DE-604 Pflanzen (DE-588)4045539-7 s Gewebekultur (DE-588)4157245-2 s In-vitro-Kultur (DE-588)4332883-0 s Gamborg, Oluf L. 1934- Sonstige (DE-588)172484707 oth Erscheint auch als Online-Ausgabe 978-3-642-79048-5 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=006779032&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
spellingShingle | Plant cell, tissue and organ culture fundamental methods Zellkultur (DE-588)4067547-6 gnd Pflanzen (DE-588)4045539-7 gnd Pflanzenzelle (DE-588)4115551-8 gnd Gewebekultur (DE-588)4157245-2 gnd In-vitro-Kultur (DE-588)4332883-0 gnd |
subject_GND | (DE-588)4067547-6 (DE-588)4045539-7 (DE-588)4115551-8 (DE-588)4157245-2 (DE-588)4332883-0 |
title | Plant cell, tissue and organ culture fundamental methods |
title_auth | Plant cell, tissue and organ culture fundamental methods |
title_exact_search | Plant cell, tissue and organ culture fundamental methods |
title_full | Plant cell, tissue and organ culture fundamental methods O. L. Gamborg ... (ed.) |
title_fullStr | Plant cell, tissue and organ culture fundamental methods O. L. Gamborg ... (ed.) |
title_full_unstemmed | Plant cell, tissue and organ culture fundamental methods O. L. Gamborg ... (ed.) |
title_short | Plant cell, tissue and organ culture |
title_sort | plant cell tissue and organ culture fundamental methods |
title_sub | fundamental methods |
topic | Zellkultur (DE-588)4067547-6 gnd Pflanzen (DE-588)4045539-7 gnd Pflanzenzelle (DE-588)4115551-8 gnd Gewebekultur (DE-588)4157245-2 gnd In-vitro-Kultur (DE-588)4332883-0 gnd |
topic_facet | Zellkultur Pflanzen Pflanzenzelle Gewebekultur In-vitro-Kultur |
url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=006779032&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
work_keys_str_mv | AT gamborgolufl plantcelltissueandorganculturefundamentalmethods |