A genetic switch: phage lambda and higher organisms
Saved in:
| Main Author: | |
|---|---|
| Format: | Book |
| Language: | English |
| Published: |
Cambridge, Mass.
Cell Press u.a.
1992
|
| Edition: | 2. ed. |
| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Physical Description: | IX, 192 S. Ill., zahlr. graph. Darst. |
| ISBN: | 0865422095 |
Staff View
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| 245 | 1 | 0 | |a A genetic switch |b phage lambda and higher organisms |c by Mark Ptashne |
| 250 | |a 2. ed. | ||
| 264 | 1 | |a Cambridge, Mass. |b Cell Press u.a. |c 1992 | |
| 300 | |a IX, 192 S. |b Ill., zahlr. graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
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| 650 | 7 | |a Bacteriofaag lambda |2 gtt | |
| 650 | 7 | |a Bacteriofago, Fago |2 larpcal | |
| 650 | 7 | |a Genetische variatie |2 gtt | |
| 650 | 7 | |a Groei |2 gtt | |
| 650 | 7 | |a Regelmechanismen |2 gtt | |
| 650 | 4 | |a Bacteriophage lambda | |
| 650 | 4 | |a Bacteriophage lambda |x genetics | |
| 650 | 4 | |a Gene Expression Regulation | |
| 650 | 4 | |a Genes, Viral | |
| 650 | 4 | |a Genetic regulation | |
| 650 | 4 | |a Viral genetics | |
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Record in the Search Index
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|---|---|
| adam_text | IMAGE 1
CONTENTS
PREFACE TO THE SECOND EDITION PREFACE
INTRODUCTION
CHAPTER ONE: THE MASTER ELEMENTS OF CONTROL
X
1
3
13
COMPONENTS OF THE SWITCH 16 DNA 16 RNA POLYMERASE 17 THE REPRESSOR 17
CRO 19
THE ACTION OF REPRESSOR AND CRO 20 NEGATIVE CONTROL 20 POSITIVE CONTROL
20
COOPERATIVITY OF REPRESSOR BINDING 22
INDUCTION-FLIPPING THE SWITCH 24
COOPERATIVITY-SWITCH STABILITY AND SENSITIVITY 27
THE EFFECT OF AUTOREGULATION 30
OTHER CASES 30
CHAPTER TWO: PROTEIN-DNA INTERACTIONS AND GENE CONTROL 33
THE OPERATOR 33
REPRESSOR 35
CRO 39
AMINO ACID-BASE PAIR INTERACTIONS 41
THE PROMOTER 44
GENE CONTROL 45
IMAGE 2
VI CONTENTS
CHAPTER THREE: CONTROL CIRCUITS-SETTING THE SWITCH 49
A BRIEF OVERVIEW OF A GROWTH 50 THE GENETIC MAP 50 CIRCULARIZATION 51
GENE EXPRESSION 52
VERY EARLY EARLY LATE INTEGRATION 53
CONTROL OF TRANSCRIPTION 54 VERY EARLY 54 EARLY 54 LATE LYTIC 56
LATE LYSOGENIC 57
THE DECISION 58
CONTROL OF INTEGRATION AND EXCISION 59 CASE 1-ESTABLISHING LYSOGENY 60
CASE 2-LYTIC GROWTH 60 CASE 3 -INDUCTION 61
OTHER PHAGES 62
THE SOS RESPONSE 62
X PATHWAYS AND CELL DEVELOPMENT 64 REGULATORY GENES 64 SWITCHES 65
PATTERNS OF GENE EXPRESSION 66
CHAPTER FOUR: HOW DO WE KNOW-THE KEY EXPERIMENTS 69
THE REPRESSOR IDEA 69 CLEAR AND VIRULENT MUTANTS 69 OBSERVATIONS
EXPLANATION
IMMUNITY AND HETEROIMMUNITY 70 OBSERVATIONS EXPLANATION ASYMMETRY IN
BACTERIAL MATING 72
OBSERVATIONS EXPLANATION
IMAGE 3
CONTENTS VII
THE REPRESSOR PROBLEM IN T HE EARLY 1960S 73
REPRESSOR ISOLATION AND D NA BINDING 74
MAKING M O RE REPRESSOR 76
THE CLAIMS OF CHAPTERS O NE A ND TWO 77 THE REPRESSOR IS COMPOSED OF TWO
GLOBULAR DOMAINS HELD TOGETHER BY A LINKER OF SOME 40 AMINO ACIDS 77 THE
REPRESSOR DIMERIZES, LARGELY THROUGH INTERACTION BETWEEN ITS CARBOXYL
DOMAINS 79
A REPRESSOR DIMER BINDS, THROUGH ITS AMINO DOMAINS, TO A 17 BASE PAIR
OPERATOR SITE 79 A SINGLE OPERATOR SITE BINDS ONE DIMER OF REPRESSOR
DINNERS FORM BEFORE DNA BINDING
THE AMINO DOMAINS CONTACT DNA
THERE ARE THREE 17 BASE PAIR REPRESSOR BINDING SITES IN THE RIGHT
OPERATOR. AT EACH SITE REPRESSOR AND CRO BIND ALONG THE SAME FACE OF THE
HELIX 85 CHEMICAL PROBES OPERATOR MUTATIONS
BINDING TO SUPERCOILED AND LINEAR DNA
REPRESSOR BINDS TO THREE SITES IN O R WITH ALTERNATE PAIRWISE
COOPERATIVITY. THE COOPERATIVITY IS MEDIATED BY INTERACTIONS BETWEEN
CARBOXYL DOMAINS OF ADJACENT DIMERS 87
IN A LYSOGEN REPRESSOR IS TYPICALLY BOUND TO O R 1 AND O R 2. THE BOUND
REPRESSORS TURN OFF RIGHTWARD TRANSCRIPTION OF CRO AND STIMULATE
LEFTWARD TRANSCRIPTION OF CL. AT HIGHER CONCENTRATIONS, REPRESSOR BINDS
TO O R 3 TO TURN OFF TRANSCRIPTION OF CL 88
CRO BINDS FIRST TO O R 3, THEN TO O R 1 AND O R 2, THEREBY FIRST TURNING
OFF P RM , THEN P R 93
SOME BACKGROUND ABOUT CRO CRO IN VIVO CRO IN VITRO
RECA CLEAVES REPRESSOR TO TRIGGER INDUCTION 96
WHEN CRO IS BOUND AT O R 3 THE SWITCH IS THROWN 97
REPRESSOR AND CRO BIND TO THE OPERATOR AS SHOWN IN FIGURES 2.6, 2.8,
2.10 AND 2.11 97 CRYSTALLOGRAPHY THE HELIX SWAP EXPERIMENT
A REPRESSOR HETERODIMER THAT BINDS TO A HYBRID OPERATOR THE ROLE OF THE
ARM OF A REPRESSOR SPECIFIC AMINO ACID-BASE PAIR CONTACTS
IMAGE 4
VIII CONTENTS
REPRESSOR ACTIVATES TRANSCRIPTION OF CL BY BINDING TO O R 2 AND
CONTACTING POLYMERASE WITH ITS AMINO DOMAIN 102 POSITIVE CONTROL MUTANTS
POSITIVE CONTROL IN VITRO
X REPRESSOR S ACTIVATING REGION TURNING A CRO INTO AN ACTIVATOR
CONCLUSION 107
CHAPTER FIVE: EUKARYOTIC GENE REGULATION-AN OVERVIEW 113
INTRODUCTION 113
EUKARYOTIC GENES AND THEIR POLYMERASES 114
ACTIVATION 115
REPRESSION 123
COOPERATIVITY 125
REGULATORS ASSEMBLED FROM NON-IDENTICAL COMPONENTS 126
SPECULATIONS ON DEVELOPMENT AND EVOLUTION 127
CAVEATS 129
CHAPTER SIX: EUKARYOTIC GENE REGULATION- EXPERIMENTS AND EXAMPLES 133
BACKGROUND 133 A TECHNICAL NOTE 137 A NOTE ON NOMENCLATURE 138
TWO FUNCTIONS OF AN ACTIVATOR: DNA BINDING AND ACTIVATION 138
DNA-BINDING STRUCTURES 140 HOMEODOMAINS 140 ZINC-REQUIRING STRUCTURES
142
ACTIVATING REGIONS 143 OCT1 AND SP1 147 MECHANISM OF ACTIVATION 148
ACTION AT A DISTANCE-LOOPING 148
ACTIVATION IN VIVO AND IN VITRO 152 STRENGTHS OF ACTIVATING REGIONS 153
NUMBER OF SITES 153
IMAGE 5
CONTENTS IX
SEPARATION OF GENE AND ACTIVATOR BINDING SITES 153
ENHANCER ACTION 153
SQUELCHING 154
REPRESSION 156
REGULATORS ASSEMBLED FROM NON-IDENTICAL COMPONENTS 157
MORE COOPERATIVITY 162
THE CASE OF EVE 164
APPENDIX ONE: DESIGNING AN EFFICIENT DNA-BINDING PROTEIN 177
SYNOPSIS 177 SPECIFIC AND NON-SPECIFIC BINDING 177 INCREASING
SPECIFICITY 179 INCREASING PROTEIN CONCENTRATION
IMPROVING SPECIFICITY DIRECTLY USING COOPERATIVITY DNA-PROTEIN
INTERACTION IN EUKARYOTES 182
APPENDIX TWO: STRONG AND WEAK INTERACTIONS 185
|
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| author | Ptashne, Mark 1940- |
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| callnumber-subject | QR - Microbiology |
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| ctrlnum | (OCoLC)25713934 (DE-599)BVBBV009784631 |
| dewey-full | 576.6482 |
| dewey-hundreds | 500 - Natural sciences and mathematics |
| dewey-ones | 576 - Genetics and evolution |
| dewey-raw | 576.6482 |
| dewey-search | 576.6482 |
| dewey-sort | 3576.6482 |
| dewey-tens | 570 - Biology |
| discipline | Biologie |
| edition | 2. ed. |
| format | Book |
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| indexdate | 2024-07-09T17:40:48Z |
| institution | BVB |
| isbn | 0865422095 |
| language | English |
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| physical | IX, 192 S. Ill., zahlr. graph. Darst. |
| publishDate | 1992 |
| publishDateSearch | 1992 |
| publishDateSort | 1992 |
| publisher | Cell Press u.a. |
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| spelling | Ptashne, Mark 1940- Verfasser (DE-588)138150850 aut A genetic switch phage lambda and higher organisms by Mark Ptashne 2. ed. Cambridge, Mass. Cell Press u.a. 1992 IX, 192 S. Ill., zahlr. graph. Darst. txt rdacontent n rdamedia nc rdacarrier Bacteriofaag lambda gtt Bacteriofago, Fago larpcal Genetische variatie gtt Groei gtt Regelmechanismen gtt Bacteriophage lambda Bacteriophage lambda genetics Gene Expression Regulation Genes, Viral Genetic regulation Viral genetics Bakteriophage Lambda (DE-588)4208901-3 gnd rswk-swf Genregulation (DE-588)4122166-7 gnd rswk-swf Bakteriophage Lambda (DE-588)4208901-3 s Genregulation (DE-588)4122166-7 s DE-604 SWB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=006472714&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Ptashne, Mark 1940- A genetic switch phage lambda and higher organisms Bacteriofaag lambda gtt Bacteriofago, Fago larpcal Genetische variatie gtt Groei gtt Regelmechanismen gtt Bacteriophage lambda Bacteriophage lambda genetics Gene Expression Regulation Genes, Viral Genetic regulation Viral genetics Bakteriophage Lambda (DE-588)4208901-3 gnd Genregulation (DE-588)4122166-7 gnd |
| subject_GND | (DE-588)4208901-3 (DE-588)4122166-7 |
| title | A genetic switch phage lambda and higher organisms |
| title_auth | A genetic switch phage lambda and higher organisms |
| title_exact_search | A genetic switch phage lambda and higher organisms |
| title_full | A genetic switch phage lambda and higher organisms by Mark Ptashne |
| title_fullStr | A genetic switch phage lambda and higher organisms by Mark Ptashne |
| title_full_unstemmed | A genetic switch phage lambda and higher organisms by Mark Ptashne |
| title_short | A genetic switch |
| title_sort | a genetic switch phage lambda and higher organisms |
| title_sub | phage lambda and higher organisms |
| topic | Bacteriofaag lambda gtt Bacteriofago, Fago larpcal Genetische variatie gtt Groei gtt Regelmechanismen gtt Bacteriophage lambda Bacteriophage lambda genetics Gene Expression Regulation Genes, Viral Genetic regulation Viral genetics Bakteriophage Lambda (DE-588)4208901-3 gnd Genregulation (DE-588)4122166-7 gnd |
| topic_facet | Bacteriofaag lambda Bacteriofago, Fago Genetische variatie Groei Regelmechanismen Bacteriophage lambda Bacteriophage lambda genetics Gene Expression Regulation Genes, Viral Genetic regulation Viral genetics Bakteriophage Lambda Genregulation |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=006472714&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT ptashnemark ageneticswitchphagelambdaandhigherorganisms |