Molecular ecology of rhizosphere microorganisms: biotechnology and the release of GMOs
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
Weinheim u.a.
VCH
1994
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XIII, 177 S. Ill. |
| ISBN: | 352730052X |
Internformat
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Datensatz im Suchindex
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| adam_text | IMAGE 1
CONTENT
PREFACE V LIST OF CONTRIBUTORS VII
1 CURRENT CHALLENGES IN INTRODUCING BENEFICIAL MICROORGANISMS INTO THE
RHIZOSPHERE 1 1.1 INTRODUCTION AND DEFINITIONS 1 1.2 RELATIONSHIP OF
ROOT COLONIZATION TO BIOCONTROL AND GROWTH PROMO-
TION 2
1.3 THE PROCESS OF COLONIZATION 3
1.4 EFFECT OF BIOTIC AND ABIOTIC FACTORS 4
1.5 BACTERIAL TRAITS CONTRIBUTING TO RHIZOSPHERE COMPETENCE 5 1.6
POPULATION DYNAMICS OF PGPR IN THE FIELD 7 1.7 RELEASE OF GENETICALLY
ENGINEERED RHIZOBACTERIA 8 1.8 MECHANISMS OF BIOLOGICAL CONTROL BY PGPR
9 1.9 INCONSISTANT PERFORMANCE OF PGPR 11 1.10 IMPROVING ROOT COLONIZING
AND BIOLOGICAL CONTROL 12 1.11 CONCLUSION 13
1.12 REFERENCES 13
2 STUDIES ON INDIGENOUS ENDOPHYTIC BACTERIA OF SWEET CORN AND COTTON 19
2.1 INTRODUCTION 19
2.2 MATERIALS AND METHODS 20
2.2.1 MEDIA 20
2.2.2 FIELD EXPERIMENTS 20 2.2.3 SAMPLE PREPARATION AND SURFACE
STERILIZATION 20 2.2.4 GROWTH CONDITIONS, BACTERIAL COUNTS AND DATA
ANALYSIS 21 2.2.5 ISOLATION AND PRESERVATION OF ENDOPHYTES 21 2.2.6
STRAIN IDENTIFICATION 21 2.3 RESULTS 22
2.3.1 POPULATION DYNAMICS 22 2.3.2 BACTERIAL IDENTIFICATION 23 2.4
DISCUSSION 24
2.5 REFERENCES 27
3 DETECTION OF INTRODUCED BACTERIA IN THE RHIZOSPHERE USING MARKER GENES
AND DNA PROBES 29 3.1 INTRODUCTION 29
3.2 METHODS 30
IMAGE 2
X CONTENT
3.2.1 SPONTANEOUS ANTIBIOTIC RESISTANCE 30 3.2.2 MARKER GENES 31 3.2.2.1
NEW METABOLIC CAPABILITY 32 3.2.2.2 HEAVY METAL RESISTANCE 33 3.2.2.3
BIOLUMINESCENCE 33 3.2.2.4 HERBICIDE RESISTANCE 33 3.2.2.5 TRANSPOSONS
CARRYING ANTIBIOTIC RESISTANCE 33 3.2.3 DNA PROBES 35 3.2.4 DETECTION
LIMITS, AMPLIFICATION AND ENRICHMENT 37 3.2.4.1 INCREASED SENSITIVITY BY
PCR AMPLIFICATION 37 3.2.4.2 ENRICHMENT 37 3.3 CASE STUDY: TRACKING LACZ
^LABELLED PSEUDOMONAS CORRUGATA IN
THE FIELD 38
3.3.1 PRE-RELEASE TESTING 39 3.3.2 FIELD RELEASE 40 3.4 THE ECOLOGICAL
FITNESS OF GENETICALLY-ENGINEERED BACTERIA 41 3.4.1 METABOLIC LOAD 41
3.4.2 REDUCED FITNESS 41 3.5 CONCLUSIONS 42
3.6 REFERENCES 44
4 IMPACT OF GEMMOS ON RHIZOSPHERE POPULATION DYNAMICS 49 4.1
INTRODUCTION 49
4.2 A MOST PROBABLE NUMBER (MPN) RECOVERY TECHNIQUE 50 4.3 THE NEED FOR
AN ECO-PHYSIOLOGICAL INDEX (EPI) 51 4.4 CONCLUSIONS 53
4.5 REFERENCES 54
5 DEVELOPING CONCEPTS IN BIOLOGICAL CONTROL: A MOLECULAR ECOLOGY
APPROACH 57 5.1 INTRODUCTION 57
5.2 SIDEROPHORE-MEDIATED COMPETITIVE EXCLUSION OF PHYTOPATHOGENS 58 5.3
EXPLOITING ANTIFUNGAL METABOLITES TO ENHANCE BIOLOGICAL CONTROL 60 5.4
STABILITY OF INTRODUCED GENES AND BIOLOGICAL CONTAINMENT SYSTEMS FOR
GMO S 61
5.5 CONCLUSION 63
5.6 REFERENCES 64
6 BIOCONTROL OF ROOT DISEASES BY PSEUDOMONAS FLUORESCENS CHAO: CURRENT
CONCEPTS AND EXPERIMENTAL APPROACHES 67 6.1 INTRODUCTION 67
6.2 MECHANISTIC STUDIES ON BIOCONTROL TRAITS OF PSEUDOMONAS FLUORESCENS
CHAO 68 6.2.1 CHEMICAL IDENTIFICATION OF EXTRACELLULAR METABOLITES 68
6.2.2 GENETIC MANIPULATION OF STRAIN CHAO 70 6.2.3 GNOTOBIOTIC SYSTEM 73
6.2.4 MUTATIONS AFFECTING BIOCONTROL EFFICACY, REGULATION OF SECONDARY
METABOLISM, AND SOME CAVEATS 75
IMAGE 3
CONTENT XI
6.2.5 INDUCED SYSTEMIC RESISTANCE IN PLANTS 77 6.2.6 GENETIC INSTABILITY
OF STRAIN CHAO: EFFECTS ON SECONDARY METABOLISM AND BIOLOGICAL CONTROL
78 6.3 ENVIRONMENTAL IMPACT OF BACTERIAL INOCULANTS 79 6.3.1
ENVIRONMENTAL MONITORING 80 6.3.2 MICROCOSMS 80 6.4 POTENTIAL
APPLICATIONS 81 6.5 CONCLUSION 82
6.6 REFERENCES 83
7 GENETIC STRATEGIES TO ENGINEER EXPRESSION SYSTEMS RESPONSIVE TO
RELEVANT ENVIRONMENTAL SIGNALS 91 7.1 INTRODUCTION 91
7.2 MINI-TRANSPOSONS AS GENETIC TOOLS 91 7.2.1 RATIONALE FOR THE
UTILIZATION OF MINI-TN5 TRANSPOSONS 92 7.2.2 A UNIVERSAL SUICIDE
DELIVERY SYSTEM 92 7.2.3 ALTERNATIVE SELECTION MARKERS 94
7.3 ENGINEERING GENE EXPRESSION WITHIN MINI-TRANSPOSONS 95 7.3.1
SELECTING AN ADEQUATE LEVEL OF TRANSCRIPTION 95 7.3.2
POST-TRANSCRIPTIONAL BOTTLENECKS 96 7.4 ENGINEERING ALKYL- AND
HALO-AROMATIC RESPONSIVE PHENOTYPES 97 7.5 OUTLOOK 99
7.6 REFERENCES 100
8 GENETIC TYPING OF MICROORGANISMS: CURRENT CONCEPTS AND FUTURE
PROSPECTS 103 8.1 INTRODUCTION 103
8.2 TECHNIQUES FOR THE ANALYSIS OF DNA SEQUENCE POLYMORPHISMS 104 8.2.1
SOUTHERN BLOT AND HYBRIDIZATION 104 8.2.2 PCR-AMPLIFICATION OF
POLYMORPHIC DNA 105 8.2.3 RIBOTYPING OF BACTERIAL STRAINS 105 8.2.4
FINGERPRINTING BY ARBITRARILY PRIMED PCR 106 8.2.5 FINGERPRINTING BY
TRNA CONSENSUS PRIMED PCR 107 8.2.6 AUTOMATED ANALYSIS OF FINGERPRINTS
109 8.3 OUTLOOK 109
8.4 REFERENCES 111
9 DEVELOPMENT OF SUBTRACTION HYBRIDIZATION PROCEDURES FOR GENERATING
STRAIN-SPECIFIC RHIZOBIUM DNA PROBES 113 9.1 INTRODUCTION 113
9.2 SYSTEM WITH BIOTINYLATED AND MERCURATED SUBTRACTER DNA 113 9.3
COMBINED SUBTRACTION HYBRIDIZATION AND PCR AMPLIFICATION PROCEDURE 114
9.3.1 TECHNICAL DETAILS 115 9.3.1.1 ISOLATION OF DNA 116
9.3.1.2 SYNTHESIS OF OLIGONUCLEOTIDES AND PREPARATION OF LINKERS 116
IMAGE 4
X II CONTENT
9.3.1.3 PREPARATION OF PROBE STRAIN DNA 117 9.3.1.4 PREPARATION OF
SUBTRACTER DNA 117 9.3.1.5 SUBTRACTION HYBRIDIZATION 117 9.3.1.6
ISOLATION OF PROBE STRAIN DNA SEQUENCES FROM THE SUBTRACTION
MIXTURE 117
9.4 RESULTS 118
9.5 CONCLUSIONS 118
9.6 REFERENCES 119
10 MOLECULAR CHARACTERIZATION AND DETECTION OF THE ACTINOMYCETE FRANKIA
IN THE ENVIRONMENT 121 10.1 INTRODUCTION 121 10.2 TAXONOMY 122 10.2.1
DNA-DNA HYBRIDIZATION DATA 122 10.2.2 SEQUENCING OF 16S RDNA GENES 123
10.3 CHARACTERIZATION OF FRANKIA 125 10.3.1 CONVENTIONAL TECHNIQUES 125
10.3.2 SEQUENCE BASED CHARACTERIZATION 126 10.3.2.1 INTERGENIC SPACERS
126 10.3.2.2 PCR/RFLP 127 10.4 DETECTION AND ENUMERATION 128
10.4.1 DETECTION OF FRANKIA IN ACTINORHIZAE 128 10.4.2 DIRECT DETECTION
OF FRANKIA PRESENT IN THE SOIL 129 10.5 CONCLUSION 130 10.6 REFERENCES
130
11 MOLECULAR ECOLOGY OF FILAMENTOUS ACTINOMYCETES IN SOIL 133 11.1
INTRODUCTION 133 11.2 LIFE-CYCLE OF STREPTOMYCETES IN SOIL 134 11.2.1
SPORE GERMINATION AND MYCELIAL DEVELOPMENT IN SOIL 135 11.2.2 MOLECULAR
MONITORING OF DIFFERENTIATION IN SOIL 136 11.3 POTENTIAL FOR GENETIC
INTERACTIONS BETWEEN ACTINOMYCETES IN SOIL 138 11.3.1 CONJUGATIVE
INTERACTIONS BETWEEN STREPTOMYCETES IN SOIL 139 11.3.2 GENE EXCHANGE
BETWEEN ACTINOMYCETES AND OTHER BACTERIA 140 11.3.3 INTERACTIONS BETWEEN
STREPTOMYCETES AND ACTINOPHAGES IN SOIL 141 11.4 DETECTION AND
EXPRESSION OF SPECIFIC GENES IN SOIL 142 11.4.1 ANTIBIOTIC RESISTANCE
GENES AND EXPRESSION OF ANTIBIOTIC PRODUCTION
GENES IN SOIL 143 11.4.2 DETECTION OF AMPLIFIED GENES IN SOIL 144 11.5
CONCLUSIONS 145 11.7 REFERENCES 146
12 SOME CONSIDERATIONS ON GENE TRANSFER BETWEEN BACTERIA IN SOIL AND
RHIZOPHERE 151 12.1 INTRODUCTION 151 12.2 SOIL AND RHIZOSPHERE AS
HABITATS FOR BACTERIA 152
IMAGE 5
CONTENT XIII
12.3 GENE TRANSFER IN SOIL AND RHIZOSPHERE 153 12.3.1 TRANSFORMATION 153
12.3.2 TRANSDUCTION 155 12.3.3 CONJUGATION 157 12.4 CONCLUDING REMARKS
159 12.5 REFERENCES 161
13 EUROPEAN COMMUNITY REGULATION FOR THE USE AND RELEASE OF GENETICALLY
MODIFIED ORGANISMS (GMOS) IN THE ENVIRONMENT 165 13.1 INTRODUCTION 165
13.2 THE INTERNATIONAL REGULATORY FRAMEWORK 167 13.3 THE EUROPEAN
COMMUNITY REGULATION 168 13.4 BIOSAFETY RESULTS OF FIELD TESTS OF GMOS
169 13.5 CONCLUDING REMARKS 171 13.6 REFERENCES 171
INDEX 175
|
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| genre | (DE-588)4143413-4 Aufsatzsammlung gnd-content (DE-588)1071861417 Konferenzschrift 1993 Cork gnd-content |
| genre_facet | Aufsatzsammlung Konferenzschrift 1993 Cork |
| id | DE-604.BV009719008 |
| illustrated | Illustrated |
| indexdate | 2024-07-09T17:39:47Z |
| institution | BVB |
| isbn | 352730052X |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-006429616 |
| oclc_num | 31598006 |
| open_access_boolean | |
| owner | DE-355 DE-BY-UBR DE-703 DE-M49 DE-BY-TUM DE-11 |
| owner_facet | DE-355 DE-BY-UBR DE-703 DE-M49 DE-BY-TUM DE-11 |
| physical | XIII, 177 S. Ill. |
| publishDate | 1994 |
| publishDateSearch | 1994 |
| publishDateSort | 1994 |
| publisher | VCH |
| record_format | marc |
| spelling | Molecular ecology of rhizosphere microorganisms biotechnology and the release of GMOs ed. by F. O'Gara ... Weinheim u.a. VCH 1994 XIII, 177 S. Ill. txt rdacontent n rdamedia nc rdacarrier Biotechnologie gtt Genetische manipulatie gtt Rhizobium gtt Microbial ecology Congresses Rhizosphere Congresses Molekularbiologie (DE-588)4039983-7 gnd rswk-swf Rhizosphäre (DE-588)4049846-3 gnd rswk-swf Mikroorganismus (DE-588)4039226-0 gnd rswk-swf Bakterien (DE-588)4004296-0 gnd rswk-swf Freisetzung (DE-588)4155333-0 gnd rswk-swf Ökologie (DE-588)4043207-5 gnd rswk-swf Transgener Organismus (DE-588)4314075-0 gnd rswk-swf Gentechnisch veränderter Organismus (DE-588)4353579-3 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content (DE-588)1071861417 Konferenzschrift 1993 Cork gnd-content Rhizosphäre (DE-588)4049846-3 s Bakterien (DE-588)4004296-0 s Transgener Organismus (DE-588)4314075-0 s DE-604 Mikroorganismus (DE-588)4039226-0 s Freisetzung (DE-588)4155333-0 s Gentechnisch veränderter Organismus (DE-588)4353579-3 s Molekularbiologie (DE-588)4039983-7 s Ökologie (DE-588)4043207-5 s O'Gara, Fergal Sonstige oth SWB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=006429616&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Molecular ecology of rhizosphere microorganisms biotechnology and the release of GMOs Biotechnologie gtt Genetische manipulatie gtt Rhizobium gtt Microbial ecology Congresses Rhizosphere Congresses Molekularbiologie (DE-588)4039983-7 gnd Rhizosphäre (DE-588)4049846-3 gnd Mikroorganismus (DE-588)4039226-0 gnd Bakterien (DE-588)4004296-0 gnd Freisetzung (DE-588)4155333-0 gnd Ökologie (DE-588)4043207-5 gnd Transgener Organismus (DE-588)4314075-0 gnd Gentechnisch veränderter Organismus (DE-588)4353579-3 gnd |
| subject_GND | (DE-588)4039983-7 (DE-588)4049846-3 (DE-588)4039226-0 (DE-588)4004296-0 (DE-588)4155333-0 (DE-588)4043207-5 (DE-588)4314075-0 (DE-588)4353579-3 (DE-588)4143413-4 (DE-588)1071861417 |
| title | Molecular ecology of rhizosphere microorganisms biotechnology and the release of GMOs |
| title_auth | Molecular ecology of rhizosphere microorganisms biotechnology and the release of GMOs |
| title_exact_search | Molecular ecology of rhizosphere microorganisms biotechnology and the release of GMOs |
| title_full | Molecular ecology of rhizosphere microorganisms biotechnology and the release of GMOs ed. by F. O'Gara ... |
| title_fullStr | Molecular ecology of rhizosphere microorganisms biotechnology and the release of GMOs ed. by F. O'Gara ... |
| title_full_unstemmed | Molecular ecology of rhizosphere microorganisms biotechnology and the release of GMOs ed. by F. O'Gara ... |
| title_short | Molecular ecology of rhizosphere microorganisms |
| title_sort | molecular ecology of rhizosphere microorganisms biotechnology and the release of gmos |
| title_sub | biotechnology and the release of GMOs |
| topic | Biotechnologie gtt Genetische manipulatie gtt Rhizobium gtt Microbial ecology Congresses Rhizosphere Congresses Molekularbiologie (DE-588)4039983-7 gnd Rhizosphäre (DE-588)4049846-3 gnd Mikroorganismus (DE-588)4039226-0 gnd Bakterien (DE-588)4004296-0 gnd Freisetzung (DE-588)4155333-0 gnd Ökologie (DE-588)4043207-5 gnd Transgener Organismus (DE-588)4314075-0 gnd Gentechnisch veränderter Organismus (DE-588)4353579-3 gnd |
| topic_facet | Biotechnologie Genetische manipulatie Rhizobium Microbial ecology Congresses Rhizosphere Congresses Molekularbiologie Rhizosphäre Mikroorganismus Bakterien Freisetzung Ökologie Transgener Organismus Gentechnisch veränderter Organismus Aufsatzsammlung Konferenzschrift 1993 Cork |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=006429616&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT ogarafergal molecularecologyofrhizospheremicroorganismsbiotechnologyandthereleaseofgmos |