Molecular genetics of yeast: a practical approach
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
Oxford u.a.
IRL Press
1994
|
| Schriftenreihe: | The practical approach series
141 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XXIV, 275 S. Ill., graph. Darst. |
| ISBN: | 0199634300 0199634297 |
Internformat
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Datensatz im Suchindex
| _version_ | 1804124066219556864 |
|---|---|
| adam_text | 3733- MOLECULAR GENETICS OF YEAST A PRACTICAL APPROACH EDITED BY JOHN R.
JOHNSTON DEPARTMENT OF BIOSCIENCE AND BIOTECHNOLOGY, UNIVERSITY OF
STRATHCJYDE, HOYAJ COJJEGE BUILDING, 204 GEORGE STREET, GLASGOW GL 1XW,
UK -AT- OXFORD UNIVERSITY PRESS OXFORD NEW YORK TOKYO CONTENTS LIST OF
CONTRIBUTORS XIX LIST OF ABBREVIATIONS XXIII 1. PROCEDURES FOR ISOLATING
YEAST DNA FOR DIFFERENT PURPOSES 1 ACHIM WACH, HORST PICK, AND PETER
PHILIPPSEN 1. INTRODUCTION 1 2. LARGE SCALE PREPARATION OF CLEAN DNA BY
CSCL DENSITY GRADIENT EQUILIBRIUM CENTRIFUGATION 2 COMMENTS ON PROTOCOL
1 4 3. ISOLATION OF YEAST DNA FROM 30 ML CULTURES 7 COMMENTS ON PROTOCOL
2 9 ALTERNATIVE MEDIUM SCALE PROCEDURE USING A COMMERCIALLY AVAILABLE
KIT 10 4. FAST SMALL SCALE ISOLATION OF YEAST TOTAL DNA 10 COMMENTS ON
PROTOCOL 3 11 5. RECOMMENDED VOLUMES FOR FURTHER EXPERIMENTAL
UTILIZATION OF DNA 12 6. INTERPRETATION OF BAND PATTERNS IN RESTRICTION
SPECTRA OF S. CEREVISIAE DNA 14 REFERENCES 16 2. CONSTRUCTION OF CLONING
AND EXPRESSION VECTORS 17 CHRISTOPHER HADFIELD 1. INTRODUCTION 17 2.
TYPES AND PROPERTIES OF YEAST VECTORS 17 2 N,M-BASED PLASMID VECTORS 18
ARS PLASMIDS 20 CENTROMERE-CONTAINING VECTORS 20 INTEGRATIVE VECTORS 20
3. BASIC VECTOR COMPONENTS 21 E. COLI PLASMID COMPONENT 21 CONTENTS
YEAST AUXOTROPHIC MARKERS 21 YEAST DOMINANT MARKERS 22 YEAST REPLICONS
22 4. BASIC VECTOR CONSTRUCTION METHODOLOGY 24 THE ISOLATION OF DNA
FRAGMENTS FROM GELS 24 CLONING MANIPULATIONS 24 5. EXPRESSION CONSTRUCTS
26 TRANSLATIONAL FUSION CONSTRUCTS 27 TRANSCRIPTIONAL FUSION CONSTRUCTS
35 TRANSCRIPTION TERMINATORS 38 6. VECTOR PURPOSE AND DESIGN 42
EFFICIENT CDNA CLONING 42 GENOMIC LIBRARY CONSTRUCTION 42 SUB-CLONING 44
EXPRESSION ALTERNATIVES 44 ACKNOWLEDGEMENTS 46 REFERENCES 46 3. COSMID
CLONING OF YEAST DNA 49 ROLF STUCKA AND HORST FELDMANN 1. INTRODUCTION
49 2. CONSTRUCTION OF YEAST COSMID LIBRARIES 49 3. COSMID VECTORS AND
PROCEDURES USED IN CLONING OF YEAST DNA 51 COSMID VECTORS 51 CLONING
PROCEDURES 53 HANDLING OF YEAST COSMID LIBRARIES 59 4. COSMID MAPPING
STRATEGIES 61 CHROMOSOMAL WALKING 61 CHROMOSOME FRAGMENTATION 62 5.
COMPLEMENTATION ANALYSIS IN YEAST USING COSMIDS 62 REFERENCES 63 4. THE
CONSTRUCTION AND USE OF CDNA LIBRARIES FOR GENETIC SELECTIONS 65 JOHN T.
MULLIGAN AND STEPHEN J. ELLEDGE 1. INTRODUCTION 65 2. CDNA CLONING
VECTORS 65 CONTENTS 3. GENETIC USES FOR REGULATED EXPRESSION LIBRARIES
67 DOSAGE-DEPENDENT SUPPRESSORS 67 DOMINANT PHENOTYPES 67 GENE ISOLATION
FROM OTHER ORGANISMS BY SELECTION IN YEAST 69 ISOLATION OF GENES BY
COMPLEMENTATION OF E. COLI MUTATIONS USING YES 70 4. CONSTRUCTION OF
CDNA LIBRARIES IN THE X YES VECTORS 70 GENERAL CONSIDERATIONS 70 CDNA
SYNTHESIS 71 CDNA CLONING 73 5. COMPLEMENTATION OF MUTANTS WITH CDNA
LIBRARIES 79 REFERENCES 81 5. PULSED FIELD GEL ELECTROPHORESIS 83 JOHN
R. JOHNSTON 1. INTRODUCTION 83 2. EQUIPMENT 83 ORTHOGONAL FIELD
ALTERNATION (OFAGE) 84 FIELD INVERSION (FIGE) 84 CONTOUR-CLAMPED
HOMOGENEOUS ELECTRIC FIELDS (CHEF) 84 ROTATING GEL (RGE) 85 TRANSVERSE
ALTERNATING FIELD (TAFE) 85 PULSED HOMOGENEOUS ORTHOGONAL FIELD (PHOGE)
85 PROGRAMMABLE AUTONOMOUSLY CONTROLLED ELECTRODE (PACE) 85 OTHER
MODIFICATIONS 85 3. PREPARATION OF HIGH MOLECULAR WEIGHT DNA 86 4. GEL
PREPARATION AND RUNS 90 5. CONCLUDING REMARKS 92 ACKNOWLEDGEMENTS 92
REFERENCES 93 6. PLASMID SHUFFLING AND MUTANT ISOLATION 97 ROBERT S.
SIKORSKI, JILL B. KEENEY, AND JEF D. BOEKE 1. INTRODUCTION 97 2. PLASMID
SHUFFLING: PRINCIPLES 97 3. PLASMID SHUFFLING: MATERIALS 98 YEAST-E.
COLI SHUTTLE VECTORS 98 YEAST STRAIN 98 MEDIA 102 BACTERIAL STRAINS 102
XI CONTENTS 4. PLASMID SHUFFLING: METHOD 103 BUILDING AN APPROPRIATE
YEAST HOST STRAIN 103 MUTAGENESIS 103 REMOVAL OF WILD-TYPE GENE BY
COUNTERSELECTION 104 IDENTIFYING INTERESTING MUTANT ALLELES 107
ACKNOWLEDGEMENTS 108 REFERENCES 108 7. TY INSERTIONAL MUTAGENESIS IN J.
N. STRATHERN, M. MASTRANGELO, L. A. RINCKEL, AND D. J. GARFINKEL 1.
INTRODUCTION 111 2. SUCCESSES 113 3. PROBLEMS 114 INSERTIONS ARE NOT
RANDOM 114 INSERTIONS CAN BE COMPLEX 115 4. FUTURE DEVELOPMENTS 115 5.
CONSIDERATIONS IN CHOOSING A METHOD 116 ACKNOWLEDGEMENTS 119 REFERENCES
119 8. HIGH EFFICIENCY TRANSFORMATION WITH LITHIUM ACETATE 121 R. DANIEL
GIETZ AND ROBIN A. WOODS 1. INTRODUCTION 121 APPLICATIONS OF HIGH
EFFICIENCY LIAC TRANSFORMATION 122 2. PREPARATION OF MEDIA AND REAGENTS
123 COMPLEX MEDIUM (YPAD) 123 SYNTHETIC COMPLETE (SC) MEDIA 123 LIAC
STOCK SOLUTION (1.0 M) 123 PEG STOCK SOLUTION, 50% (W/V) 124
SINGLE-STRANDED CARRIER DNA 124 3. GROWTH OF YEAST 126 4. STANDARD
TRANSFORMATION PROCEDURE 128 5. PROCEDURES FOR OPTIMIZING THE PROTOCOL
FOR SPECIFIC STRAIN/PLASMID COMBINATIONS 130 6. MAXIMIZING THE NUMBER OF
TRANSFORMANTS 130 XII CONTENTS 7. QUICK AND EASY TRANSFORMATION
PROCEDURE 132 8. REMEDIES FOR PROBLEMS WHICH CAN AFFECT THE EFFICIENCY
OF LIAC/SSDNA/PEG TRANSFORMATION 132 STRAINS AND PLASMIDS 132
EVAPORATION OF PEG 133 REASSOCIATION AND DEGRADATION OF THE
SINGLE-STRANDED CARRIER DNA 133 PREPARATION OF THE SC-MINUS MEDIUM 133
FAILURE TO OBTAIN ANY TRANSFORMANTS 133 ACKNOWLEDGEMENTS 133 REFERENCES
134 9. MEASUREMENT OF TRANSCRIPTION 135 PETER W. PIPER 1. INTRODUCTION
135 2. YEAST PROMOTERS 135 3. PREPARATION OF UNDEGRADED RNA 137
PRECAUTIONS TO MINIMIZE RNASE CONTAMINATION 137 RAPID RNA ISOLATION 138
ENRICHMENT FOR POLYADENYLATED RNA 141 DESIGNING NORTHERN BLOTTING
EXPERIMENTS 143 ACKNOWLEDGEMENTS 145 REFERENCES 145 10. MEASUREMENT OF
MRNA STABILITY 147 ALISTAIR J. P. BROWN 1. INTRODUCTION ; 147 2. RNA
ISOLATION PROCEDURES 148 PREPARATION OF RNASE-FREE MATERIALS 148 STORAGE
OF CELLS BEFORE RNA PREPARATION 148 PREPARATION OF RNA 149 3.
MEASUREMENT OF MRNA HALF-LIVES 152 IN VIVO RADIOLABELLING PROCEDURES 152
THE USE OF TRANSCRIPTIONAL INHIBITORS 153 THE USE OF THE RNA POLYMERASE
II MUTATION, RPBL-1 155 THE USE OF SPECIFIC PROMOTERS TO STOP
TRANSCRIPTION 156 4. MRNA QUANTIFICATION 157 ACKNOWLEDGEMENTS 159
REFERENCES 159 XIII CONTENTS 11. PRODUCTION OF FOREIGN PROTEINS AT HIGH
LEVEL I6I GERARD LOISON 1. INTRODUCTION 161 2. TRANSFORMATION METHODS
162 3. SELECTABLE MARKERS 162 INTRODUCTION 162 DEFECTIVE LEU2 MARKER 162
DEFECTIVE URA3-A MARKER 163 THE URA3 FURL AUTOSELECTION SYSTEM 164 4.
VECTORS 167 INTRODUCTION 167 EXPRESSION VECTORS FOR CIR + CELLS 167
EXPRESSION VECTORS FOR CIR CELLS 168 5. EXPRESSION BLOCK 169 PROMOTERS
169 THE MRNA LEADER SEQUENCE 171 THE FOREIGN PROTEIN-ENCODING SEQUENCE
172 TRANSCRIPTION TERMINATION SIGNALS AND MRNA STABILITY 175 6. PROTEIN
STABILITY 175 7. BASIC METHODS FOR ANALYSING PROTEIN PRODUCTION 176
ACKNOWLEDGEMENTS 178 REFERENCES 178 12. CELL-FREE TRANSLATION OF NATURAL
AND SYNTHETIC MRNAS IN YEAST LYSATES ISI MICK F. TUITE, DELIN ZHU, AND
ALAN D. HARTLEY 1. INTRODUCTION ; 181 2. PREPARATION OF CELL-FREE
LYSATES 182 3. PREPARATION OF YEAST MRNA TEMPLATES 187 YEAST MRNA
STRUCTURE 187 PREPARATION OF YEAST MRNA 188 IN VITRO TRANSCRIPTS 189 4.
CELL-FREE TRANSLATION OF NATURAL AND SYNTHETIC MRNAS 192 5. IN VITRO
TRANSLOCATION SYSTEMS 196 6. ALTERNATIVES TO IN VITRO TRANSLATION:
TRANSIENT EXPRESSION SYSTEMS 199 ACKNOWLEDGEMENTS 199 REFERENCES 199 XIV
CONTENTS 13. VIRUS-LIKE PARTICLES: TY RETROTRANSPOSONS 203 ALAN J.
KINGSMARI, SALLY E. ADAMS, NIGEL R. BURNS, ENCARNA MARTIN-RENDON, GEMMA
MARFANY, DOUGLAS W. HURD, AND SUSAN M. KINGSMAN 1. INTRODUCTION 203 2.
THE BIOLOGY OF TYL 203 3. TY EXPRESSION VECTORS 205 4. THE PURIFICATION
OF TY-VLPS 208 5. REVERSE TRANSCRIPTION AND INTEGRATION IN VITRO 212
ACKNOWLEDGEMENTS 215 REFERENCES 215 14. THE KL KILLER TOXIN: MOLECULAR
AND GENETIC APPLICATIONS TO SECRETION AND CELL SURFACE ASSEMBLY 217
JEFFREY L. BROWN, TERRY ROEMER, MARC LUSSIER, ANNE-MARIE SDICU, AND
HOWARD BUSSEY 1. INTRODUCTION 217 2. KILLER TOXIN STRUCTURE AND FUNCTION
218 KILLER TOXIN PROCESSING 218 CELL SURFACE INTERACTIONS 218 LETHAL
ACTION 219 IMMUNITY 219 3. KRE CELL WALL MUTANTS 219 4. APPLICATIONS 220
HETEROLOGOUS SYSTEMS 220 NEW TOXINS 221 5. KILLER TOXIN METHODOLOGY 221
ISOLATION OF YEAST KL KILLER TOXIN 221 TOXIN COMPETITION ASSAYS 223
SEEDED PLATE TESTS 224 KILLER PROCESSING 225 6. USING KILLER TOXIN TO
IDENTIFY NEW GENES 226 ISOLATING KILLER-RESISTANT MUTANTS 226 CLONING
GENES BY FUNCTIONAL COMPLEMENTATION OF KILLER-RESISTANT MUTANTS 227 7.
CELL WALL ANALYSES 228 P(1^3) AND P(L-*6) GLUCAN 228 CELL WALL DYES 230
XV CONTENTS ACKNOWLEDGEMENTS 231 REFERENCES 231 15. IMMUNO-ELECTRON
MICROSCOPY 233 MICHAEL W. CLARK 1. INTRODUCTION 233 OBJECTIVES 233
COMPROMISE 234 2. MATERIALS 234 CHEMICALS AND EQUIPMENT 234 BUFFERS AND
STAINING SOLUTIONS 235 3. TRADITIONAL FIXATION 236 CELL GROWTH AND
HARVESTING 236 PRIMARY FIXATION 236 PERMEABILIZATION OF THE CELL WALL
237 POST-FIXATION 237 DEHYDRATION AND EMBEDDING 237 4. SECTION
PREPARATION AND IMMUNO-GOLD STAINING 239 SECTION ETCHING 239 IMMUNO-GOLD
LABELLING OF THE SECTIONS 240 5. SYSTEMATIC MODIFICATION OF THE
PROCEDURE TO INCREASE THE ANTIBODY SIGNAL 242 REDUCTION OF THE EXPOSURE
TO THE FIXATIVES 244 CHANGING THE EMBEDDING RESIN 245 6. FUTURE
DIRECTIONS 245 REFERENCES 246 16. INDUSTRIAL SACCHAROMYCES YEASTS 247
MORTEN C. KIELLAND-BRANDT 1. INTRODUCTION BREEDING AND RESEARCH 2. MODEL
SYSTEMS FERMENTER MODELS BIOLOGICAL MODELS 3. GENETIC ANALYSIS AND
CROSS-BREEDING SPORULATION SPORE GERMINATION MATING GENETIC
CHARACTERIZATION OF INDIVIDUAL CHROMOSOMES 247 247 248 248 248 249 250
250 253 254 XVI CONTENTS 4. GENETIC TRANSFORMATION 255 CHROMOSOMAL
INTEGRATION 256 5. CONCLUDING REMARKS 258 REFERENCES 258 APPENDIX 1.
MORE COMMON MEDIA AND BUFFERS 261 APPENDIX 2. ADDRESSES OF SUPPLIERS 267
INDEX 27I XVLL
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| owner | DE-355 DE-BY-UBR DE-91G DE-BY-TUM DE-M49 DE-BY-TUM DE-29T DE-703 DE-20 |
| owner_facet | DE-355 DE-BY-UBR DE-91G DE-BY-TUM DE-M49 DE-BY-TUM DE-29T DE-703 DE-20 |
| physical | XXIV, 275 S. Ill., graph. Darst. |
| publishDate | 1994 |
| publishDateSearch | 1994 |
| publishDateSort | 1994 |
| publisher | IRL Press |
| record_format | marc |
| series | The practical approach series |
| series2 | The practical approach series |
| spelling | Molecular genetics of yeast a practical approach ed. by John R. Johnston Oxford u.a. IRL Press 1994 XXIV, 275 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier The practical approach series 141 Genetic Techniques Genetics, Experimental Molecular Biology Molecular genetics Technique Saccharomyces cerevisiae Genetics Saccharomyces cerevisiae genetics Yeasts genetics Molekulargenetik (DE-588)4039987-4 gnd rswk-swf Methode (DE-588)4038971-6 gnd rswk-swf Hefeartige Pilze (DE-588)4230509-3 gnd rswk-swf Saccharomyces cerevisiae (DE-588)4178812-6 gnd rswk-swf Saccharomyces cerevisiae (DE-588)4178812-6 s Molekulargenetik (DE-588)4039987-4 s Methode (DE-588)4038971-6 s DE-604 Hefeartige Pilze (DE-588)4230509-3 s Johnston, John R. Sonstige oth The practical approach series 141 (DE-604)BV007921321 141 GBV Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=006428506&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Molecular genetics of yeast a practical approach The practical approach series Genetic Techniques Genetics, Experimental Molecular Biology Molecular genetics Technique Saccharomyces cerevisiae Genetics Saccharomyces cerevisiae genetics Yeasts genetics Molekulargenetik (DE-588)4039987-4 gnd Methode (DE-588)4038971-6 gnd Hefeartige Pilze (DE-588)4230509-3 gnd Saccharomyces cerevisiae (DE-588)4178812-6 gnd |
| subject_GND | (DE-588)4039987-4 (DE-588)4038971-6 (DE-588)4230509-3 (DE-588)4178812-6 |
| title | Molecular genetics of yeast a practical approach |
| title_auth | Molecular genetics of yeast a practical approach |
| title_exact_search | Molecular genetics of yeast a practical approach |
| title_full | Molecular genetics of yeast a practical approach ed. by John R. Johnston |
| title_fullStr | Molecular genetics of yeast a practical approach ed. by John R. Johnston |
| title_full_unstemmed | Molecular genetics of yeast a practical approach ed. by John R. Johnston |
| title_short | Molecular genetics of yeast |
| title_sort | molecular genetics of yeast a practical approach |
| title_sub | a practical approach |
| topic | Genetic Techniques Genetics, Experimental Molecular Biology Molecular genetics Technique Saccharomyces cerevisiae Genetics Saccharomyces cerevisiae genetics Yeasts genetics Molekulargenetik (DE-588)4039987-4 gnd Methode (DE-588)4038971-6 gnd Hefeartige Pilze (DE-588)4230509-3 gnd Saccharomyces cerevisiae (DE-588)4178812-6 gnd |
| topic_facet | Genetic Techniques Genetics, Experimental Molecular Biology Molecular genetics Technique Saccharomyces cerevisiae Genetics Saccharomyces cerevisiae genetics Yeasts genetics Molekulargenetik Methode Hefeartige Pilze Saccharomyces cerevisiae |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=006428506&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| volume_link | (DE-604)BV007921321 |
| work_keys_str_mv | AT johnstonjohnr moleculargeneticsofyeastapracticalapproach |