Effects of glutamate induced obesity and feeding with a low protein diet on the metabolism of fructose 2,6-bisphosphate in the rat:
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| Main Author: | |
|---|---|
| Format: | Book |
| Language: | English |
| Published: |
1994
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| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Item Description: | Leipzig, Univ., Diss., 1994 |
| Physical Description: | 118, [30] Bl. graph. Darst. |
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Record in the Search Index
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CONTENTS
PAGE
1. INTRODUCTION
1
1.1. GENERAL
1
1.2. ROLE OF FRU-2,6-P
2 IN THE CONTROL OF
GLYCOLYSIS AND GLUCONEOGENESIS 2
1.3. ENZYMES CATALYZING THE SYNTHESIS AND
DEGRADATION OF FRU-2,6-P2 4
1.4. SHORT-TERM REGULATION OF FRU-2,6-P
2
METABOLISM 6
1.5. LONG-TERM REGULATION OF FRU-2,6-P
2
METABOLISM 9
1.6. EFFECTS OF DIET COMPOSITION AND OBESITY
ON THE METABOLISM OF FRU-2,6-P2 _ 11
1.7. OBJECTIVES OF THE WORK 13
2. MATERIALS AND METHODS 14
2.1. MATERIALS 14
2.1.1. KITS 15
2.1.2. SOLUTIONS, BUFFER AND MEDIA 15
2.2. METHODS 22
2.2.1. TREATMENT OF ANIMALS 22
2.2.2. HOMOGENIZATION OF TISSUES 23
2.2.3. ASSAYS OF ENZYME ACTIVITIES 24
2.2.4. PREPARATION OF POTATO TUBER 6-PHOSPHO-
FRUCTO-1-KINASE 25
2.2.5. PROTEIN DETERMINATION 26
2.2.6. DETERMINATION OF METABOLITE CONCENTRATIONS 26
2.2.7. GLUCOSE TOLERANCE TEST 27
2.2.8. PRODUCTION OF A POLYCLONAL ANTISERUM
AGAINST RAT SERUM ALBUMIN AND QUANTITATION
OF ALBUMIN USING IMMUNO-ELECTROPHORESIS 27
2.2.9. DIETHYLPYROCARBONATE TREATMENT OF WATER 27
BIBLIOGRAFISCHE INFORMATIONEN
HTTP://D-NB.INFO/941332764
28
2.2.10. ISOLATION OF RNA
2.2.11. RNA ELECTROPHORESIS 30
2.2.12. TRANSFER ONTO AN UNCHARGED NYLON MEMBRANE
(NORTHERN TRANSFER) AND FIXATION OF RNA. 31
2.2.13. RNA DOT BLOTS 33
2.2.14. SYNTHESIS AND PURIFICATION OF OLIGODEOXY-
RIBONUCLEOTIDES 33
2.2.15. LABELING OF OLIGODEOXYRIBONUCLEOTIDES
USED AS PROBES M NORTHERN ANALYSIS 36
2.2.16. POLYACRYLAMIDE GEL ELECTROPHORESIS (RAGE)
OF OLIGONUCLEOTIDES 36
2.2.17. REVERSE TRANSCRIPTION 36
2.2.18. LIGASE-FREE SUBCLONING OF POLYMERASE CHAIN
REACTION (PCR) PRODUCTS 37
2.2.19. PURIFICATION AND ANALYSIS OF PLASMIDS 40
2.2.20. FLUORIMETRIC DETERMINATION OF DNA
CONCENTRATION 41
2.2.21. DNA SEQUENCING 41
2.2.22. SYNTHESIS OF RNA PROBES BY
IN VITRO
TRANSCRIPTION 42
2.2.23. SYNTHESIS OF POLY-(A)
+-SENSE RNAS TO BE
USED AS ADDITIONAL INTERNAL CONTROLS IN
NORTHERN ANALYSIS 43
2.2.24. NORTHERN ANALYSIS AND DOT BLOTTING 44
2.2.25. STATISTICAL ANALYSIS 45
3. RESULTS 46
3.1. METABOLISM OF FRU-2,6-P
2 IN TISSUES
OF NORMAL FED AND STARVED RATS 46
3.1.1. EFFECTS OF TOTAL STARVATION AND RE-
FEEDING ON BODY AND ORGAN WEIGHTS AS
WELL AS ON THE ACTIVITIES OF
GLYCOLYTIC ENZYMES OF NORMAL RATS 46
3.1.2. EFFECTS OF TOTAL STARVATION AND
REFEEDING ON THE METABOLISM OF
FRU-2,6-P2 IN THE LIVER
3.1.3. CONCENTRATIONS OF METABOLITES AND
ACTIVITIES OF ENZYMES IN EXTRAHEPATIC
48
TISSUES DURING STARVATION AND REFEEDING 52
3.1.4. ACTIVITIES OF THE BIFUNCTIONAL ENZYME
IN RAT HEART, MUSCLE AND BRAIN 56
3.2. THE EFFECT OF LOW-PROTEIN DIET
ON THE METABOLISM OF FRU-2,6-P2
IN GROWING RATS 59
3.2.1. FOOD INTAKE AND GROWTH CURVES OF
ANIMALS FED WITH A LOW-PROTEIN DIET 59
3.2.2. EFFECTS OF LOW-PROTEIN DIET ON THE CON
CENTRATIONS OF GLUCOSE, ALBUMIN, AND
INSULIN IN RAT SERUM 63
3.2.3. ENZYME ACTIVITIES AND TISSUE LEVELS
OF METABOLITES IN GROWING RATS FED
WITH A LOW-PROTEIN DIET 64
3.3. METABOLISM OF FRU-2,6-P
2 IN TISSUES OF
GLUTAMATE INDUCED OBESE RATS 68
3.3.1. SOMATIC CHARACTERIZATION OF GLUTAMATE
INDUCED OBESE RATS (GOR) 68
3.3.2. FRU-2,6-P
2 LEVELS IN DIFFERENT TISSUES OF
GLUTAMATE INDUCED OBESE RATS WITH
AND WITHOUT DIABETES 72
3.3.3. ENZYME ACTIVITIES AND METABOLITE CONCEN
TRATIONS IN TISSUES OF GOR 75
3.4. DETERMINATION OF THE MRNAS OF PFK-2/
F2,6PASE ISOZYMES 80
3.4.1. PREPARATION, QUANTITATION AND QUALITY
CONTROL OF RNA 80
3.4.2. SYNTHESIS AND APPLICATION OF OLIGODEOXY-
RIBONUCLEOTIDE PROBES FOR PFK-2/F2,6PASE
MRNAS 81
3.4.3. RNA PROBES FOR STUDIES OF THE GENE
EXPRESSION OF PFK-2/F2,6PASE ISOZYMES 83
3.4.3.1. REVERSE TRANSCRIPTION AND POLYMERASE
CHAIN REACTIONS 83
3.4.3.2. LIGASE-FREE SUBCLONING OF PCR PRODUCTS 84
3.4.3.3. PROBE SYNTHESIS BY DIRECT TRANSFORMATION
WITH PCR PRODUCTS 86
3.4.3.4. SYNTHESIS OF RNA PROBES BY
IN VITRO
TRANSCRIPTION 91
3.4.3.5. SPECIFICITY ANALYSIS OF THE RNA PROBES 92
3.4.3.6. STUDIES OF GENE EXPRESSION OF PFK-2/
F2,6PASE ISOZYMES IN GLUTAMATE INDUCED
OBESE AND LOW PROTEIN FED RATS
4. DISCUSSION
4.1. CHARACTERIZATION OF FRU-2,6-P
2 METABOLISM
4.2. DETERMINATION BY NORTHERN ANALYSIS
AND DOT BLOTTING OF MRNAS OF PFK-2/
F2,6PASE ISOZYMES
4.3. ALTERATIONS OF FRU-2,6-P
2 METABOLISM
IN NORMAL RAT TISSUES BY STARVATION AND
REFEEDING
4.4. THE EFFECTS OF FEEDING GROWING RATS WITH
A LOW-PROTEIN DIET ON THE METABOLISM
OF FRU-2,6-P2
4.5. METABOLISM OF FRU-2,6-P
2 IN TISSUES
OF GLUTAMATE INDUCED OBESE RATS
REFERENCES |
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| spelling | Berhanu Temesgen Verfasser (DE-588)114137080 aut Effects of glutamate induced obesity and feeding with a low protein diet on the metabolism of fructose 2,6-bisphosphate in the rat submitted by Berhanu Temesgen 1994 118, [30] Bl. graph. Darst. txt rdacontent n rdamedia nc rdacarrier Leipzig, Univ., Diss., 1994 Ratte (DE-588)4177011-0 gnd rswk-swf Mangelernährung (DE-588)4138143-9 gnd rswk-swf Fettsucht (DE-588)4016953-4 gnd rswk-swf Glucosestoffwechsel (DE-588)4129732-5 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Ratte (DE-588)4177011-0 s Fettsucht (DE-588)4016953-4 s Glucosestoffwechsel (DE-588)4129732-5 s DE-604 Mangelernährung (DE-588)4138143-9 s DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=006418272&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Berhanu Temesgen Effects of glutamate induced obesity and feeding with a low protein diet on the metabolism of fructose 2,6-bisphosphate in the rat Ratte (DE-588)4177011-0 gnd Mangelernährung (DE-588)4138143-9 gnd Fettsucht (DE-588)4016953-4 gnd Glucosestoffwechsel (DE-588)4129732-5 gnd |
| subject_GND | (DE-588)4177011-0 (DE-588)4138143-9 (DE-588)4016953-4 (DE-588)4129732-5 (DE-588)4113937-9 |
| title | Effects of glutamate induced obesity and feeding with a low protein diet on the metabolism of fructose 2,6-bisphosphate in the rat |
| title_auth | Effects of glutamate induced obesity and feeding with a low protein diet on the metabolism of fructose 2,6-bisphosphate in the rat |
| title_exact_search | Effects of glutamate induced obesity and feeding with a low protein diet on the metabolism of fructose 2,6-bisphosphate in the rat |
| title_full | Effects of glutamate induced obesity and feeding with a low protein diet on the metabolism of fructose 2,6-bisphosphate in the rat submitted by Berhanu Temesgen |
| title_fullStr | Effects of glutamate induced obesity and feeding with a low protein diet on the metabolism of fructose 2,6-bisphosphate in the rat submitted by Berhanu Temesgen |
| title_full_unstemmed | Effects of glutamate induced obesity and feeding with a low protein diet on the metabolism of fructose 2,6-bisphosphate in the rat submitted by Berhanu Temesgen |
| title_short | Effects of glutamate induced obesity and feeding with a low protein diet on the metabolism of fructose 2,6-bisphosphate in the rat |
| title_sort | effects of glutamate induced obesity and feeding with a low protein diet on the metabolism of fructose 2 6 bisphosphate in the rat |
| topic | Ratte (DE-588)4177011-0 gnd Mangelernährung (DE-588)4138143-9 gnd Fettsucht (DE-588)4016953-4 gnd Glucosestoffwechsel (DE-588)4129732-5 gnd |
| topic_facet | Ratte Mangelernährung Fettsucht Glucosestoffwechsel Hochschulschrift |
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