Protocols for nucleic acid analysis by nonradioactive probes:
This book provides an exceptionally useful collection of tried and tested protocols for nucleic acid analysis that avoid the use of radioisotopes. Southern (i.e., DNA) and Northern (i.e., RNA) blotting protocols are covered in detail, from how to isolate good quality nucleic acids from plant and ani...
Saved in:
| Format: | Book |
|---|---|
| Language: | English |
| Published: |
Totowa, NJ
Humana Press
1994
|
| Series: | Methods in molecular biology
28 |
| Subjects: | |
| Summary: | This book provides an exceptionally useful collection of tried and tested protocols for nucleic acid analysis that avoid the use of radioisotopes. Southern (i.e., DNA) and Northern (i.e., RNA) blotting protocols are covered in detail, from how to isolate good quality nucleic acids from plant and animal sources, to the use of the widest range of probe systems (involving digoxigenin, biotin, fluorescein, horseradish peroxidase labeling, and colorimetric and chemiluminescent detection). Other techniques treated in detail include hybridization in situ to chromosomes and transcribed RNA, newer and less conventional technologies, such as Randomly Amplified Polymorphic DNA (RAPD), reverse dot blots, bacterial identification using magnetic head and dipstick assays, and for the first time in detail, Nucleic Acid Sequence-Based Amplification. |
| Physical Description: | XII, 268 S. Ill., graph. Darst. |
| ISBN: | 089603254X |
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| 490 | 1 | |a Methods in molecular biology |v 28 | |
| 520 | 3 | |a This book provides an exceptionally useful collection of tried and tested protocols for nucleic acid analysis that avoid the use of radioisotopes. Southern (i.e., DNA) and Northern (i.e., RNA) blotting protocols are covered in detail, from how to isolate good quality nucleic acids from plant and animal sources, to the use of the widest range of probe systems (involving digoxigenin, biotin, fluorescein, horseradish peroxidase labeling, and colorimetric and chemiluminescent detection). Other techniques treated in detail include hybridization in situ to chromosomes and transcribed RNA, newer and less conventional technologies, such as Randomly Amplified Polymorphic DNA (RAPD), reverse dot blots, bacterial identification using magnetic head and dipstick assays, and for the first time in detail, Nucleic Acid Sequence-Based Amplification. | |
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| id | DE-604.BV009682094 |
| illustrated | Illustrated |
| indexdate | 2024-07-09T17:39:07Z |
| institution | BVB |
| isbn | 089603254X |
| language | English |
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| physical | XII, 268 S. Ill., graph. Darst. |
| publishDate | 1994 |
| publishDateSearch | 1994 |
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| publisher | Humana Press |
| record_format | marc |
| series | Methods in molecular biology |
| series2 | Methods in molecular biology |
| spelling | Protocols for nucleic acid analysis by nonradioactive probes ed. by Peter G. Isaac Totowa, NJ Humana Press 1994 XII, 268 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 28 This book provides an exceptionally useful collection of tried and tested protocols for nucleic acid analysis that avoid the use of radioisotopes. Southern (i.e., DNA) and Northern (i.e., RNA) blotting protocols are covered in detail, from how to isolate good quality nucleic acids from plant and animal sources, to the use of the widest range of probe systems (involving digoxigenin, biotin, fluorescein, horseradish peroxidase labeling, and colorimetric and chemiluminescent detection). Other techniques treated in detail include hybridization in situ to chromosomes and transcribed RNA, newer and less conventional technologies, such as Randomly Amplified Polymorphic DNA (RAPD), reverse dot blots, bacterial identification using magnetic head and dipstick assays, and for the first time in detail, Nucleic Acid Sequence-Based Amplification. DNA Sequencing cabt Chemische analyses gtt Nucleïnezuren gtt Wetenschappelijke technieken gtt Wissenschaftliches Arbeiten Nucleic Acid Probes Nucleic Acids analysis Nucleic acid probes Nucleic acids Analysis Analyse (DE-588)4122795-5 gnd rswk-swf Nucleinsäuren (DE-588)4172117-2 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Nucleinsäuren (DE-588)4172117-2 s Analyse (DE-588)4122795-5 s DE-604 Isaac, Peter G. Sonstige oth Methods in molecular biology 28 (DE-604)BV035362695 28 |
| spellingShingle | Protocols for nucleic acid analysis by nonradioactive probes Methods in molecular biology DNA Sequencing cabt Chemische analyses gtt Nucleïnezuren gtt Wetenschappelijke technieken gtt Wissenschaftliches Arbeiten Nucleic Acid Probes Nucleic Acids analysis Nucleic acid probes Nucleic acids Analysis Analyse (DE-588)4122795-5 gnd Nucleinsäuren (DE-588)4172117-2 gnd |
| subject_GND | (DE-588)4122795-5 (DE-588)4172117-2 (DE-588)4143413-4 |
| title | Protocols for nucleic acid analysis by nonradioactive probes |
| title_auth | Protocols for nucleic acid analysis by nonradioactive probes |
| title_exact_search | Protocols for nucleic acid analysis by nonradioactive probes |
| title_full | Protocols for nucleic acid analysis by nonradioactive probes ed. by Peter G. Isaac |
| title_fullStr | Protocols for nucleic acid analysis by nonradioactive probes ed. by Peter G. Isaac |
| title_full_unstemmed | Protocols for nucleic acid analysis by nonradioactive probes ed. by Peter G. Isaac |
| title_short | Protocols for nucleic acid analysis by nonradioactive probes |
| title_sort | protocols for nucleic acid analysis by nonradioactive probes |
| topic | DNA Sequencing cabt Chemische analyses gtt Nucleïnezuren gtt Wetenschappelijke technieken gtt Wissenschaftliches Arbeiten Nucleic Acid Probes Nucleic Acids analysis Nucleic acid probes Nucleic acids Analysis Analyse (DE-588)4122795-5 gnd Nucleinsäuren (DE-588)4172117-2 gnd |
| topic_facet | DNA Sequencing Chemische analyses Nucleïnezuren Wetenschappelijke technieken Wissenschaftliches Arbeiten Nucleic Acid Probes Nucleic Acids analysis Nucleic acid probes Nucleic acids Analysis Analyse Nucleinsäuren Aufsatzsammlung |
| volume_link | (DE-604)BV035362695 |
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