Organelles:
Saved in:
| Main Author: | |
|---|---|
| Format: | Book |
| Language: | English |
| Published: |
Basingstoke u.a.
Macmillan
1989
|
| Edition: | 1. publ. |
| Series: | Macmillan molecular biology series
|
| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Physical Description: | XVII, 202 S. Ill., graph. Darst. |
| ISBN: | 0333442431 033344244X |
Staff View
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Record in the Search Index
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| adam_text | IMAGE 1
CONTENTS
SERIES EDITOR S PREFACE XI
ACKNOWLEDGEMENTS XII
INTRODUCTION XIII
1 ULTRASTRUCTURE OF THE CELL 1
1.1 THE VIEW THROUGH THE LIGHT MICROSCOPE 1
1.1.1 CELLS VARY ENORMOUSLY IN THEIR SIZE AND SHAPE 1 1.1.2 LIGHT
MICROSCOPY REVEALS THE ESSENTIAL FEATURES OF THE CELL 2
1.1.3 CERTAIN STAINS HIGHLIGHT SUBCELLULAR ORGANELLES 3 1.2 THE VIEW
THROUGH THE ELECTRON MICROSCOPE 4
1.2.1 THE ELECTRON MICROSCOPE REVEALS THE ULTRASTRUCTURE OF THE CELL 4
1.2.2 BIOCHEMICAL TECHNIQUES COMPLEMENT ELECTRON MICROSCOPY 6
1.3 MEMBRANES DIVIDE THE CELL INTO COMPARTMENTS 8
1.3.1 THE FLUID-MOSAIC MODEL DESCRIBES THE STRUCTURE OF BIOLOGICAL
MEMBRANES 8
1.3.2 COMPARTMENTALISATION WITHIN CELLS ENHANCES FUNCTION 9
1.3.3 SUBCELLULAR MEMBRANES REPRESENT PERMEABILITY BARRIERS 10
1-4 THE CYTOSOL IS A STRUCTURED AQUEOUS COMPARTMENT 12 1.4.1 THE CYTOSOL
IS THE SITE OF SEVERAL METABOLIC PATHWAYS 12
1.4.2 THE CYTOSOL ALSO CONTAINS ORGANISED STRUCTURES 13 1-5 PRINCIPLES
BEHIND SUBCELLULAR ARCHITECTURE 14
1.5.1 SPECIFIC FUNCTIONS IMPLY SPECIFIC COMPONENTS 14 1.5.2 ORGANELLES
INTERACT DYNAMICALLY 15
1-6 SUMMARY 17
1-7 STUDY QUESTIONS 17
1-8 FURTHER READING 18
2 THE EXPERIMENTAL STUDY OF ORGANELLES 19
2-1 IMAGING METHODS 19
2.1.1 LIGHT MICROSCOPY 20
IMAGE 2
VI CONTENTS
2.1.2 ELECTRON MICROSCOPY 21
2.1.3 DIFFRACTION METHODS 23
2.2 BIOCHEMICAL AND IMMUNOCHEMICAL METHODS 24
2.2.1 CENTRIFUGATION 25
2.2.2 CELL CULTURE 27
2.2.3 ELECTROPHORESIS 27
2.2.4 CHROMATOGRAPHY 30
2.2.5 BIOSYNTHESIS 32
2.2.6 MEMBRANE TRANSPORT 32
2.2.7 IMMUNOCHEMICAL METHODS 34
2.3 RECOMBINANT DNA METHODS 35
2.4 SUMMARY 37
2.5 STUDY QUESTIONS 38
2.6 FURTHER READING 38
3 THE NUCLEUS 40
3.1 THE NUCLEUS: CONTROL CENTRE OF THE CELL 40
3.1.1 THE NUCLEUS CONTAINS MOST OF THE CELL S DNA 40 3.1.2 THE NUCLEUS
HAS A DOUBLE MEMBRANE 43
3.1.3 THE PRESENCE OF THE NUCLEAR MEMBRANES HAS IMPORTANT IMPLICATIONS
FOR GENE EXPRESSION 44 3.2 THE NUCLEAR ENVELOPE 45
3.2.1 THE OUTER MEMBRANE IS CONTINUOUS WITH THE ENDOPLASMIC RETICULUM 45
3.2.2 THE INNER MEMBRANE SURROUNDS A FIBROUS PROTEIN MESHWORK, THE
LAMINA 46
3.2.3 PORES IN THE NUCLEAR ENVELOPE CONNECT THE NUCLEOPLASM WITH THE
CYTOSOL 47
3.3 THE NUCLEOLUS: SITE OF THE BIOGENESIS OF RIBOSOMES 48
3.3.1 THE NUCLEOLUS FORMS A MORPHOLOGICALLY DISTINCT REGION OF THE
NUCLEOPLASM 50
3.3.2 RIBOSOMAL SUBUNITS ARE ASSEMBLED IN THE NUCLEOLUS 51 3.4 HOW IS
DNA PACKAGED WITHIN THE NUCLEUS? 52
3.4.1 FOLDING OF DNA GENERATES SUCCESSIVE HIGHER-ORDER STRUCTURES 53
3.4.2 GENE EXPRESSION INVOLVES OPENING UP THE CONDENSED DNA 54
3.5 THE NUCLEUS AND CELL DIVISION 55
3.5.1 CHROMOSOMES UNDERGO DUPLICATION AND FURTHER CONDENSATION DURING
CELL DIVISION 55
3.5.2 THE SISTER CHROMATIDS ARE SEPARATED BY THE MITOTIC SPINDLE 56
3.5.3 THE NUCLEAR ENVELOPE AND THE NUCLEOLUS DISASSEMBLE DURING MITOSIS
57
3.6 SUMMARY 57
3.7 STUDY QUESTIONS 58
3.8 FURTHER READING 58
4 THE ENDOPLASMIC RETICULUM 60
4.1 INTRODUCTION 60
IMAGE 3
CONTENTS VLL
THE ROUGH ENDOPLASMIC RETICULUM (ER) 62
4.2.1 MOST NASCENT SECRETORY PROTEINS HAVE AN IV-TERMINAL SIGNAL PEPTIDE
63
4.2.2 CO-TRANSLATIONAL TRANSLOCATION INVOLVES A SIGNAL RECOGNITION
PARTICLE AND ITS RECEPTOR 65
4.2.3 NUMEROUS POST-TRANSLATIONAL MODIFICATIONS OCCUR IN THE LUMEN OF
THE ROUGH ER 67
THE SMOOTH ENDOPLASMIC RETICULUM 69
4.3.1 THE SMOOTH ER SYNTHESISES MOST OF THE CELL S MEMBRANE LIPIDS 69
4.3.2 AN UNIQUE ELECTRON TRANSPORT SYSTEM CATALYSES HYDROXYLATION
REACTIONS 70
4.3.3 DETOXIFICATION OCCURS IN THE SMOOTH ER 72
4.3.4 THE SMOOTH ER SEQUESTERS CALCIUM IONS 72
MEMBRANE BIOGENESIS IN THE ENDOPLASMIC RETICULUM 74 SUMMARY 77
STUDY QUESTIONS 77
FURTHER READING 78
THE GOLGI COMPLEX 80
INTRODUCTION 80
METABOLIC PROCESSING IN THE GOLGI COMPLEX 83
5.2.1 OLIGOSACCHARIDE CHAINS OF GLYCOPROTEINS UNDERGO TRIMMING AND
FURTHER GLYCOSYLATION 83
5.2.2 OTHER IMPORTANT BIOSYNTHETIC REACTIONS TAKE PLACI IN THE GOLGI
COMPLEX 87
INTRACELLULAR PROTEIN SORTING BY THE GOLGI COMPLEX 88 5.3.1 PROTEINS
WITH VARYING DESTINATIONS LEAVE THE TRANS GOLGI NETWORK 88
5.3.2 VESICLES MEDIATE THE INTRACELLULAR TRAFFIC OF SECRETORY PROTEINS
90
SUMMARY 92
STUDY QUESTIONS 93
FURTHER READING 93
THE LYSOSOME 94
INTRODUCTION 94
THE MORPHOLOGY AND LIFE-HISTORY OF LYSOSOMES 96
6.2.1 LYSOSOMES FORM A STRUCTURALLY HETEROGENEOUS CLAS OF ORGANELLES 96
6.2.2 THE LYSOSOME FAMILY HAS A DIVERSE LIFE-HISTORY 97 THE COMPOSITION
OF LYSOSOMES 100
6.3.1 THE LYSOSOMAL MEMBRANE HAS DISTINCTIVE COMPONENTS, DISTINCTIVE
PROPERTIES 100
6.3.2 THE LYSOSOMAL MATRIX IS A STORE-HOUSE OF ACID HYDROLASES AND OTHER
PROTEINS 102
THE BIOGENESIS OF LYSOSOMES 104
6.4.1 THE GENES FOR SEVERAL LYSOSOMAL ENZYMES HAVE BEEN CLONED 104
IMAGE 4
VTLL CONTENTS
6.4.2 LYSOSOMAL ENZYMES ARE SYNTHESISED AS LARGER
PRECURSORS 105
6.4.3 A PH-SENSITIVE RECEPTOR DELIVERS ACID HYDROLASES TO PRIMARY
LYSOSOMES 106
6.4.4 PRIMARY LYSOSOMES ARISE FROM A TRANS-GO GI NETWORK 106
6.5 THE PHYSIOLOGICAL FUNCTIONS OF LYSOSOMES 107
6.5.1 DEGRADATION OF EXTRACELLULAR MATERIAL AIDS THE CELL S ECONOMY 107
6.5.2 SOME CELLS ARE SPECIALISED FOR LYSOSOMAL DIGESTION 108 6.5.3
LYSOSOMES ARE INVOLVED IN THE TURNOVER OF THE CELL S OWN CONSTITUENTS
109
6.6 T HE PATHOLOGY OF LYSOSOMES 109
6.6.1 LYSOSOMAL STORAGE DISORDERS ARE PROGRESSIVE, GENERALISED, GENETIC
DISEASES 110
6.6.2 SOME CAUSES OF LYSOSOMAL PATHOLOGY ARE ENVIRONMENTAL IN NATURE 112
6.7 SUMMARY 113
6.8 STUDY QUESTIONS 114
6.9 FURTHER READING 114
7 THE P E R O X I S O ME 116
7.1 INTRODUCTION 116
7.2 STRUCTURE AND FUNCTIONS OF PEROXISOMES 118
7.2.1 PEROXISOMES ARE CATALASE-CONTAINING VESICLES 118 7.2.2 PEROXISOMES
ARE INVOLVED IN LIPID DEGRADATION AND BIOSYNTHESIS 120
7.2.3 PEROXISOMES PLAY A SPECIAL ROLE IN PLANTS 121
7.2.4 PEROXISOMAL FUNCTIONS ARE DISRUPTED IN CERTAIN GENETIC DISEASES
124
7.3 BIOGENESIS OF PEROXISOMES 125
7.4 SUMMARY 128
7.5 STUDY QUESTIONS 128
7.6 FURTHER READING 128
8 THE M I T O C H O N D R I ON 130
8.1 INTRODUCTION 130
8.1.1 MORPHOLOGY AND DISTRIBUTION OF MITOCHONDRIA 130 8.1.2 T HE
MITOCHONDRION S CONTRIBUTION TO METABOLISM 133 8.2 POWERHOUSE OF THE
CELL , 134
8.2.1 REDUCED COENZYMES ARE OXIDISED VIA THE ELECTRON TRANSPORT CHAIN
135
8.2.2 A PROTON GRADIENT DRIVES A TP SYNTHESIS 140
8.3 MITOCHONDRIAL GENETICS 144
8.3.1 MITOCHONDRIA HAVE AN UNIQUE GENETIC SYSTEM 144
8.3.2 MITOCHONDRIAL MUTANTS HAVE PROVED INFORMATIVE 147 8.4 BIOGENESIS
AND EVOLUTION OF MITOCHONDRIA 149
8.4.1 MOST MITOCHONDRIAL PROTEINS ARE IMPORTED POST-TRANSLATIONALLY 150
IMAGE 5
CONTENTS
8.4.2 HOW IS MITOCHONDRIAL GENE EXPRESSION CONTROLLED? 153 8.4.3 HOW DID
MITOCHONDRIA EVOLVE? 154
8.5 SUMMARY 155
8.6 STUDY QUESTIONS 156
8.7 FURTHER READING 156
9 THE CHLOROPLAST 158
9.1 INTRODUCTION 158
9.2 ULTRASTRUCTURE OF THE CHLOROPLAST 159
9.2.1 THE CHLOROPLAST CONTAINS THREE DISTINCT MEMBRANES 159 9.2.2 THE
CHLOROPLAST ENVELOPES RESEMBLE MITOCHONDRIAL MEMBRANES 160
9.3 ORGANISATION OF THE LIGHT-HARVESTING MACHINERY 161
9.3.1 LIGHT-HARVESTING PIGMENTS FUNNEL EXCITATION ENERGY TO AN UNIQUE
CHLOROPHYLL MOLECULE 162 9.3.2 PHOTOSYSTEM I TRANSFERS ELECTRONS TO
NADP+ 163 9.3.3 PHOTOSYSTEM II DONATES ELECTRONS TO PHOTOSYSTEM I 163
9.3.4 THE TWO PHOTOSYSTEMS ARE FUNCTIONALLY
INTERCONNECTED 163
9.3.5 THE Z-SCHEME COMPONENTS FORM THREE MEMBRANE- SPANNING COMPLEXES
164
9.3.6 PHOTOSYSTEM I AND PHOTOSYSTEM II ARE ASYMMETRICALLY DISTRIBUTED
ALONG THE THYLAKOID MEMBRANE 165
9.3.7 ELECTRON TRANSPORT GENERATES A PROTON GRADIENT 167 9.3.8 THE
PROTON GRADIENT ACROSS THE THYLAKOID MEMBRANE DRIVES ATP SYNTHESIS 168
9.3.9 PHOTOSYSTEM II IS RESPONSIBLE FOR THE FORMATION OF OXYGEN FROM
WATER 169
9.4 ORGANISATION OF CARBON-FIXATION METABOLISM 170
9.4.1 TRIOSE PHOSPHATES ARE EXPORTED TO THE CYTOSOL 171 9.4.2 THE INNER
ENVELOPE CONTAINS OTHER TRANSLOCATORS 172 9.4.3 OTHER METABOLIC
REACTIONS ARE LOCALISED WITHIN CHLOROPLASTS 172
9.5 BIOGENESIS AND EVOLUTION OF CHLOROPLASTS 173
9.5.1 CYANOBACTERIA-LIKE ORGANELLES ( CYANELLES ) RESEMBLE PRIMITIVE
CHLOROPLASTS 173
9.5.2 CHLOROPLASTS ARISE FROM PRE-EXISTING ORGANELLES 174 9.5.3 THE
CHLOROPLAST GENOME CODES FOR RIBOSOMAL COMPONENTS AND
PHOTOSYNTHESIS-RELATED PROTEINS 174 9.5.4 THE CHLOROPLAST IMPORTS
PROTEINS SYNTHESISED ON
CYTOPLASMIC RIBOSOMES 176
9.6 SUMMARY 177
9.7 STUDY QUESTIONS 178
9.8 FURTHER READING 178
10 DYNAMIC INTERACTIONS BETWEEN ORGANELLES 180
10.1 INTRODUCTION 180
10.2 RECEPTOR-MEDIATED ENDOCYTOSIS 180
10.3 THE RECEPTOR FOR LOW-DENSITY LIPOPROTEINS 185
IMAGE 6
CONTENTS
10.4 FUTURE PERSPECTIVES 188
10.5 SUMMARY 189
10.6 FURTHER READING 190
APPENDIX A: ABBREVIATIONS 191
APPENDIX B: ANSWERS TO STUDY QUESTIONS 193
INDEX 198 I
|
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| id | DE-604.BV009258048 |
| illustrated | Illustrated |
| indexdate | 2024-07-09T17:34:01Z |
| institution | BVB |
| isbn | 0333442431 033344244X |
| language | English |
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| open_access_boolean | |
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| owner_facet | DE-29T |
| physical | XVII, 202 S. Ill., graph. Darst. |
| publishDate | 1989 |
| publishDateSearch | 1989 |
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| publisher | Macmillan |
| record_format | marc |
| series2 | Macmillan molecular biology series |
| spelling | Carroll, Mark Verfasser aut Organelles 1. publ. Basingstoke u.a. Macmillan 1989 XVII, 202 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Macmillan molecular biology series Cell organelles Cytologie (DE-588)4070177-3 gnd rswk-swf Organell (DE-588)4043772-3 gnd rswk-swf Cytologie (DE-588)4070177-3 s DE-604 Organell (DE-588)4043772-3 s SWB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=006160142&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Carroll, Mark Organelles Cell organelles Cytologie (DE-588)4070177-3 gnd Organell (DE-588)4043772-3 gnd |
| subject_GND | (DE-588)4070177-3 (DE-588)4043772-3 |
| title | Organelles |
| title_auth | Organelles |
| title_exact_search | Organelles |
| title_full | Organelles |
| title_fullStr | Organelles |
| title_full_unstemmed | Organelles |
| title_short | Organelles |
| title_sort | organelles |
| topic | Cell organelles Cytologie (DE-588)4070177-3 gnd Organell (DE-588)4043772-3 gnd |
| topic_facet | Cell organelles Cytologie Organell |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=006160142&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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