A practical guide to ELISA:
Saved in:
| Main Author: | |
|---|---|
| Format: | Book |
| Language: | English |
| Published: |
Oxford u.a.
Pergamon Press
1991
|
| Edition: | 1. ed. |
| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Item Description: | Literaturverz. S. 91 - 96 |
| Physical Description: | XI, 115 S. Ill., graph. Darst. |
| ISBN: | 0080375081 0080375073 |
Staff View
MARC
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| 100 | 1 | |a Kemeny, D. M. |e Verfasser |4 aut | |
| 245 | 1 | 0 | |a A practical guide to ELISA |c by D. M. Kemeny |
| 250 | |a 1. ed. | ||
| 264 | 1 | |a Oxford u.a. |b Pergamon Press |c 1991 | |
| 300 | |a XI, 115 S. |b Ill., graph. Darst. | ||
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| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 500 | |a Literaturverz. S. 91 - 96 | ||
| 650 | 7 | |a Immunoassay |2 gtt | |
| 650 | 4 | |a Enzyme-Linked Immunosorbent Assay |x methods | |
| 650 | 4 | |a Enzyme-linked immunosorbent assay | |
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Record in the Search Index
| _version_ | 1804123317698822144 |
|---|---|
| adam_text | IMAGE 1
ACKNWLEDGEMENTS
PREFACE 1. IMMUNE CELLS AND ANTIBODIES
LYMPHOCYTES B CELLS T CELLS ANTIBODIES
IGA IGD IGE
IGG IGM AFFINITY
AVIDITY
2. IMMUNOASSAYS PRECIPITATION ASSAYS GEL DIFFUSION ASSAYS
ELECTROPHORESIS RADIOIMMUNOASSAY (RIA) AND ENZYME IMMUNOASSAY (EIA)
SEPARATION VERSUS SEPARATION-FREE ASSAYS COMPETITIVE VERSUS
NON-COMPETITIVE ASSAYS SOLID VERSUS FLUID-PHASE ASSAYS OTHER
IMMUNOASSAYS
NEPHELOMETRY AGGLUTINATION ASSAYS
3. ELISA DESIGN NON-COMPETITIVE COATED PLATE COMPETITIVE
ANTIGEN ANTIBODY
IN SITU HISTOCHEMICAL ELISA PLAQUE DOTBLOT IMMUNOBLOT
XI 1
1 1 1 2 3 3 3 3 5 5 5
7 7 8
10 15 15 16 18 20
20 20
21 22 22 25 25 26
26 26 27 28 28
IMAGE 2
CONTENTS
4. THE SOLID-PHASE SUPPORT AND THE COATING ANTIGEN OR ANTIBODY
HIGH-CAPACITY MATERIALS AGAROSE PARTICLES AND CELLULOSE PAPER DISCS
NITROCELLULOSE PAPER LOW-CAPACITY MATERIALS MICROTITRE PLATES PINS
COATING CONDITIONS DIRECT COATING INDIRECT COATING
PLATE MODIFICATION GLUTARALDEHYDE COATING ANTIGEN OR ANTIBODY ANTIGEN
ANTIBODY
REAGENT HANDLING TESTING COMMERCIAL PRODUCTS STORAGE
5. ENZYME-LABELLED DETECTOR AND SUBSTRATE ENZYME LABELS DETECTORS
ANTIBODY
ANTIGEN
CHOICE OF ENZYME AND SUBSTRATE ALKALINE PHOSPHATASE (EC 3.1.3.1)
HORSERADISH PEROXIDASE (EC 1.11.1.7) 3-D-GALACTOSIDASE (EC 3.2.1.23)
OTHER ENZYMES AND SUBSTRATES METHOD OF CONJUGATION CHEMICAL COUPLING
PURIFICATION OF CONJUGATE
STORAGE
6. QUANTITATION WHY QUANTIFY? FIXED CUT-OFF REFERENCE CURVES
WHAT ARE THEY? HOW TO PLOT WHAT SHAPE CURVE SHOULD YOU GET? WHAT IS THE
LOWER LIMIT? THE EFFECT OF AFFINITY TITRE
CHOICE OF END POINT
31
32 32 32 33 33 34 35
35 37 40 40 40 40 41 44
44 44
45 45 45 46 49 49
50 52 53 53 53
53 54 54
57 57 58 58 58 60 60 61 61 62
63
VI
IMAGE 3
CONTENTS
REFERENCE MATERIALS PREPARATION OF REFERENCE MATERIAL ASSIGNING UNITS
STANDARDS MONITORING PERFORMANCE
QUALITY CONTROLS COEFFICIENT OF VARIATION PARALLELISM DATA HANDLING
7. ASSAY OPTIMISATION AND TROUBLE SHOOTING CHOICE OF SOLID-PHASE
PREPARATION OF SOLID-PHASE METHOD OF TESTING
CONCENTRATION CHOICE OF BUFFER AND PH TIME BLOCKING VACANT PROTEIN
BINDING SITES ON THE PLATE ASSAY CONDITIONS
VOLUME TEMPERATURE MOLARITY AND PH ADDITIVES INCUBATION TIME SAMPLE
LABELLED DETECTOR
COMPETITIVE ASSAYS NON-COMPETITIVE ASSAYS SUBSTRATE TROUBLE SHOOTING
ASSAY DESIGN SHORT CIRCUITS CROSS-REACTIONS PLATES BATCH-TO-BATCH
VARIATION BLOCKING EDGE EFFECTS REAGENTS BATCH-TO-BATCH VARIATION
PRETREATMENT DILUENT ABSORPTION
8. FUTURE DEVELOPMENTS SENSITIVITY SPEED CONCENTRATION
63 63 63 64
65 65 65 66
67
69 70 70 70 70
72 72 72 73
73 73 74 74 74 74
75 75 76 77 77 77
77 78 78 78 78 79 79
79 79 79 79
81 81 82 82
IMAGE 4
CONTENTS
REACTIVE AREA 82
SIGNAL 84
CONVENIENCE 84
CLINIC/OFFICE TESTS 85
SELF/HOME TESTS 85
ON-SITE TESTS 86
THE TECHNOLOGY 86
DIP-STICKS 86
IMMUNOFILTRATION 86
SELF-CONTAINED IMMUNOASSAY 87
AUTOMATION 87
BIBLIOGRAPHY 91
APPENDIX OF METHODS 97
|
| any_adam_object | 1 |
| author | Kemeny, D. M. |
| author_facet | Kemeny, D. M. |
| author_role | aut |
| author_sort | Kemeny, D. M. |
| author_variant | d m k dm dmk |
| building | Verbundindex |
| bvnumber | BV008978935 |
| callnumber-first | Q - Science |
| callnumber-label | QP519 |
| callnumber-raw | QP519.9.E48 |
| callnumber-search | QP519.9.E48 |
| callnumber-sort | QP 3519.9 E48 |
| callnumber-subject | QP - Physiology |
| classification_rvk | WC 4350 WC 4370 |
| ctrlnum | (OCoLC)22385822 (DE-599)BVBBV008978935 |
| dewey-full | 616.07/9 |
| dewey-hundreds | 600 - Technology (Applied sciences) |
| dewey-ones | 616 - Diseases |
| dewey-raw | 616.07/9 |
| dewey-search | 616.07/9 |
| dewey-sort | 3616.07 19 |
| dewey-tens | 610 - Medicine and health |
| discipline | Biologie Medizin |
| edition | 1. ed. |
| format | Book |
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| id | DE-604.BV008978935 |
| illustrated | Illustrated |
| indexdate | 2024-07-09T17:27:51Z |
| institution | BVB |
| isbn | 0080375081 0080375073 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-005929719 |
| oclc_num | 22385822 |
| open_access_boolean | |
| owner | DE-29T DE-29 DE-19 DE-BY-UBM DE-11 |
| owner_facet | DE-29T DE-29 DE-19 DE-BY-UBM DE-11 |
| physical | XI, 115 S. Ill., graph. Darst. |
| publishDate | 1991 |
| publishDateSearch | 1991 |
| publishDateSort | 1991 |
| publisher | Pergamon Press |
| record_format | marc |
| spelling | Kemeny, D. M. Verfasser aut A practical guide to ELISA by D. M. Kemeny 1. ed. Oxford u.a. Pergamon Press 1991 XI, 115 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Literaturverz. S. 91 - 96 Immunoassay gtt Enzyme-Linked Immunosorbent Assay methods Enzyme-linked immunosorbent assay Enzyme-linked immunosorbent assay (DE-588)4152476-7 gnd rswk-swf Labormedizin (DE-588)4073931-4 gnd rswk-swf Enzyme-linked immunosorbent assay (DE-588)4152476-7 s Labormedizin (DE-588)4073931-4 s 1\p DE-604 SWB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=005929719&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis 1\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk |
| spellingShingle | Kemeny, D. M. A practical guide to ELISA Immunoassay gtt Enzyme-Linked Immunosorbent Assay methods Enzyme-linked immunosorbent assay Enzyme-linked immunosorbent assay (DE-588)4152476-7 gnd Labormedizin (DE-588)4073931-4 gnd |
| subject_GND | (DE-588)4152476-7 (DE-588)4073931-4 |
| title | A practical guide to ELISA |
| title_auth | A practical guide to ELISA |
| title_exact_search | A practical guide to ELISA |
| title_full | A practical guide to ELISA by D. M. Kemeny |
| title_fullStr | A practical guide to ELISA by D. M. Kemeny |
| title_full_unstemmed | A practical guide to ELISA by D. M. Kemeny |
| title_short | A practical guide to ELISA |
| title_sort | a practical guide to elisa |
| topic | Immunoassay gtt Enzyme-Linked Immunosorbent Assay methods Enzyme-linked immunosorbent assay Enzyme-linked immunosorbent assay (DE-588)4152476-7 gnd Labormedizin (DE-588)4073931-4 gnd |
| topic_facet | Immunoassay Enzyme-Linked Immunosorbent Assay methods Enzyme-linked immunosorbent assay Labormedizin |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=005929719&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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