The dynamics of electrophoresis:
Saved in:
| Format: | Book |
|---|---|
| Language: | English |
| Published: |
Weinheim u. a.
VCH
1992
|
| Series: | Electrophoresis library
|
| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Physical Description: | XIV, 236 S. graph. Darst. |
| ISBN: | 352728379X 1560811927 |
Staff View
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| adam_text | THE DYNAMICS OF ELECTROPHORESIS BY R. A. MOSHER, D. A. SAVILLE, W.
THORMANN VCH WEINHEIM * NEW YORK * BASEL * CAMBRIDGE CONTENTS LIST OF
ABBREVIATIONS XV 1 INTRODUCTION 1 CONTRIBUTED BY M. BIER 1.1
ELECTROPHORESIS 1 1.2 IMPORTANCE OF ELECTROPHORESIS 2 1.3 THE
KOHLRAUSCH-HITTORF CONSTRAINTS AND THE NATURE OF ELECTROPHORETIC
BOUNDARIES 4 1.4 CLASSIFICATION OF ELECTROPHORETIC PROCESSES 6 1.5
READER S GUIDE - BOOK OVERVIEW 9 1.6 REFERENCES 10 2 THE THEORY OF
ELECTROPHORETIC SEPARATIONS 11 D.A. SAVILLE 2.0 INTRODUCTION 11 2.1 THE
GOUY-CHAPMAN THEORY OF THE ELECTRIC DOUBLE-LAYER 12 2.2 THE
ELECTROPHORETIC MOBILITY OF MACROIONS AND PARTICLES 15 2.3
ELECTROOSMOSIS 18 2.4 ION MOBILITY AND ELECTRICAL CONDUCTION IN LIQUIDS
19 2.4.1 CURRENT CARRIED BY SMALL IONS 20 2.4.2 CURRENT CARRIED BY
PROTEINS 21 2.5 THE GENERAL THEORY 23 2.5.1 BASIC PRINCIPLES 23 2.5.2
CHEMICAL REACTIONS 24 2.5.3 CONSERVATION RELATIONS 27 2.5.4 SCALE
ANALYSIS 28 2.5.5 THE SIMPLIFIED MODEL IN DIMENSIONLESS FORM 30 2.5.6
BOUNDARY CONDITIONS 32 2.6 APPLICATION OF THE MODEL TO STEADY STATE
BOUNDARY MIGRATION 34 2.6.1 ANALYSIS OF A THREE-COMPONENT SYSTEM 35
2.6.2 BOUNDARY STRUCTURE FOR THE FULLY IONIZED THREE-COMPONENT SYSTEM 38
2.6.3 ANALYSIS OF A SYSTEM WITH TWO BOUNDARIES 39 2.6.4 BOUNDARY
STRUCTURE WITH PARTIALLY IONIZED SOLUTES 42 XII CONTENTS 2.6.5
REGULATING FUNCTIONS FOR WEAK ELECTROLYTES 47 2.7 THE EFFECTS OF
ELECTROOSMOSIS ON THE STRUCTURE OF AN ISOTACHOPHORETIC BOUNDARY 48 2.8
NUMERICAL IMPLEMENTATION OF THE MODEL 53 2.8.1 REFORMULATION OF THE
MATHEMATICAL MODEL 54 2.8.2 NUMERICAL TREATMENT OF THE PARTIAL
DIFFERENTIAL EQUATIONS 57 2.9 A MODEL FOR THE ELECTROPHORESIS OF
PROTEINS 60 2.10 CONCLUDING REMARKS CONCERNING THE THEORY 65 2.11
NOMENCLATURE 66 2.12 REFERENCES 69 3 INTRODUCTION TO COMPUTER MODELING
AND EXPERIMENTAL VALIDATION 71 R. A. MOSHER AND W. THORMANN 3.1 COMPUTER
SIMULATION 71 3.1.1 INTRODUCTION 71 3.1.2 THE SIMPLIFIED MATHEMATICAL
MODEL FOR LOW MOLECULAR MASS COMPONENTS 72 3.1.2.1 MASS TRANSPORT: THE
FLUX EQUATION 72 3.1.2.2 THE ASSUMPTION OF LOCAL ELECTRONEUTRALITY 73
3.1.2.3 THE TREATMENT OF WEAK AND STRONG ACIDS AND BASES 74 3.1.2.4
CONSERVATION OF MASS 75 3.1.2.5 CONSERVATION OF CHARGE 75 3.1.3 THE
TREATMENT OF PROTEINS 75 3.1.3.1 IONIZATION BEHAVIOR 75 3.1.3.2 MASS
TRANSPORT 76 3.1.3.3 CONSERVATION OF CHARGE AND CONDUCTIVITY 76 3.1.3.4
LOCAL ELECTRONEUTRALITY 77 3.1.4 PERFORMING A SIMULATION 77 3.1.4.1
INPUTS REQUIRED 77 3.1.4.2 THE OUTPUT 78 3.1.4.3 AN EXAMPLE 78 3.2
MONITORING THE DYNAMICS OF ELECTROPHORETIC PROCESSES 81 3.2.1
INTRODUCTION 81 3.2.2 THE TACHOPHOR APPARATUS 81 3.2.3 THE CAPSCAN
APPARATUS 82 3.2.4 THE ELPHOR VAP 22 85 3.3 DISCUSSION 87 3.4 REFERENCES
88 CONTENTS XIII 4 MOVING BOUNDARY ELECTROPHORESIS 89 R.A. MOSHER AND W.
THORMANN 4.1 INTRODUCTION: TISELIUS MOVING BOUNDARY ELECTROPHORESIS 89
4.2 THE MOVING BOUNDARY SEPARATION AND REGULATING PRINCIPLES 91 4.3
DISCUSSION 98 4.4 REFERENCES 100 5 ZONE ELECTROPHORESIS 103 W. THORMANN
AND R.A. MOSHER 5.1 INTRODUCTION: THE PRINCIPLE OF ZONE ELECTROPHORESIS
103 5.2 SAMPLE DYNAMICS IN ZONE ELECTROPHORESIS 104 5.2.1 MIGRATION
DYNAMICS OF ONE SAMPLE COMPONENT 105 5.2.2 SEPARATION DYNAMICS OF TWO
SAMPLE COMPONENTS 108 5.2.3 INFLUENCE OF SAMPLE COMPOSITION AND THE
REGULATING PRINCIPLE 112 5.2.4 DISCONTINUOUS ZONE ELECTROPHORESIS 114
5.2.5 MIGRATION DYNAMICS OF A PROTEIN SAMPLE 119 5.3 DISCUSSION 120 5.4
REFERENCES 122 6 ISOTACHOPHORESIS 125 W. THORMANN AND R. A. MOSHER 6.1
INTRODUCTION: THE PRINCIPLE OF ISOTACHOPHORESIS 125 6.2 CHARACTERISTICS
OF ISOTACHOPHORESIS 129 6.2.1 THE CONCEPT OF MOBILITY IN
ISOTACHOPHORESIS 129 6.2.2 THE STEADY ISOTACHOPHORETIC STATE 130 6.2.3
REQUIREMENTS FOR ISOTACHOPHORESIS 132 6.2.4 SEPARATION OF TWO COMPONENTS
133 6.2.5 SEPARATION CAPACITY 134 6.2.6 SEPARABILITY 135 6.3 DESCRIPTION
OF ISOTACHOPHORESIS BY COMPUTER MODELING 137 6.3.1 THE DISCONTINUOUS
ELECTROLYTE SYSTEM FOR ISOTACHOPHORESIS: THE LEADER/TERMINATOR BACKBONE
137 6.3.2 CONDENSATION OF A SAMPLE CONSTITUENT IN A MOVING BOUNDARY:
ESTABLISHMENT OF AN ISOTACHOPHORESIS ZONE 140 6.3.3 ILLUSTRATION OF THE
REGULATING PRINCIPLE 143 6.3.4 DYNAMICS OF MULTIPLE-ZONE
ISOTACHOPHEROGRAMS 145 6.3.5 THE ISOTACHOPHORETIC BEHAVIOR OF PROTEINS
150 XIV CONTENTS 6.3.6 SAMPLE APPLICATION, IMPURITIES AND SPECIAL CASES
156 6.4 SUMMARY 158 6.5 REFERENCES 159 LIST OF SYMBOLS 161 7 ISOELECTRIC
FOCUSING 163 R. A. MOSHER AND W. THORMANN 7.1 INTRODUCTION 163 7.2
ACID-BASE CHEMISTRY OF AMPHOLYTES 164 7.3 CHARACTERISTICS OF GOOD
CARRIER AMPHOLYTES 167 7.4 FUNDAMENTAL CONCEPTS 170 7.4.1 THE BEHAVIOR
OF AN AMPHOLYTE IN THE PRESENCE OF A PH GRADIENT AND AN ELECTRIC FIELD
170 7.4.2 THE DIFFERENTIAL EQUATION OF ISOELECTRIC FOCUSING 171 7.5
NATURAL PH GRADIENTS 172 7.5.1 STEADY STATE DISTRIBUTIONS OF TWO
IDEALIZED AMPHOLYTES 173 7.5.2 STEADY STATE DISTRIBUTIONS OF REAL
COMPOUNDS 177 7.5.3 THE MECHANISM OF NATURAL PH GRADIENT FORMATION 182
7.5.4 THE IMPACT OF POOR AMPHOLYTES AND WEAK ELECTROLYTES 189 7.5.5
CONDITIONS FOR A STEPLESS PH COURSE 192 7.5.6 ARE PH GRADIENTS ALWAYS
MONOTONIC? 193 7.6 INSTABILITIES ASSOCIATED WITH NATURAL PH GRADIENTS
194 7.6.1 THE PLATEAU PHENOMENON 194 7.6.2 PH GRADIENT DRIFTS 196
7.6.2.1 CATHODIC DRIFT 196 7.6.2.2 ANODIC DRIFT 202 7.6.2.3 SYMMETRICAL
DRIFT 204 7.6.2.4 EXPERIMENTALLY OBSERVED DRIFTS 207 7.7 PH GRADIENTS
FORMED WITH NONAMPHOTERIC BUFFERS 208 7.7.1 SYSTEMS IN FREE SOLUTION 209
7.7.2 IMMOBILIZED PH GRADIENTS 217 7.8 THE ISOELECTRIC FOCUSING OF
PROTEINS IN NATURAL PH GRADIENTS 221 7.8.1 TWO COMPONENT SYSTEMS 221
7.8.2 THE GLUTAMIC ACID/HISTIDINE/ARGININE SYSTEM 221 7.8.3 THE GLUTAMIC
ACID/CYCLOSERINE/ARGININE SYSTEM 224 7.8.4 MORE COMPLEX SYSTEMS 226 7.9
SUMMARY 228 7.10 REFERENCES 229 LIST OF SYMBOLS 231 INDEX 233
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| isbn | 352728379X 1560811927 |
| language | English |
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| physical | XIV, 236 S. graph. Darst. |
| publishDate | 1992 |
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| spelling | The dynamics of electrophoresis by R. A. Mosher ; D. A. Saville ; W. Thormann Weinheim u. a. VCH 1992 XIV, 236 S. graph. Darst. txt rdacontent n rdamedia nc rdacarrier Electrophoresis library Computersimulaties gtt Elektroforese gtt Electrophoresis Models, Chemical Computersimulation (DE-588)4148259-1 gnd rswk-swf Elektrophorese (DE-588)4014373-9 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Elektrophorese (DE-588)4014373-9 s DE-604 Computersimulation (DE-588)4148259-1 s Mosher, Richard A. Sonstige oth Saville, Dudley A. Sonstige oth Thormann, Wolfgang Sonstige oth HEBIS Datenaustausch Darmstadt application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=005457136&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | The dynamics of electrophoresis Computersimulaties gtt Elektroforese gtt Electrophoresis Models, Chemical Computersimulation (DE-588)4148259-1 gnd Elektrophorese (DE-588)4014373-9 gnd |
| subject_GND | (DE-588)4148259-1 (DE-588)4014373-9 (DE-588)4143413-4 |
| title | The dynamics of electrophoresis |
| title_auth | The dynamics of electrophoresis |
| title_exact_search | The dynamics of electrophoresis |
| title_full | The dynamics of electrophoresis by R. A. Mosher ; D. A. Saville ; W. Thormann |
| title_fullStr | The dynamics of electrophoresis by R. A. Mosher ; D. A. Saville ; W. Thormann |
| title_full_unstemmed | The dynamics of electrophoresis by R. A. Mosher ; D. A. Saville ; W. Thormann |
| title_short | The dynamics of electrophoresis |
| title_sort | the dynamics of electrophoresis |
| topic | Computersimulaties gtt Elektroforese gtt Electrophoresis Models, Chemical Computersimulation (DE-588)4148259-1 gnd Elektrophorese (DE-588)4014373-9 gnd |
| topic_facet | Computersimulaties Elektroforese Electrophoresis Models, Chemical Computersimulation Elektrophorese Aufsatzsammlung |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=005457136&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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