Practical capillary electrophoresis:
Saved in:
| Main Author: | |
|---|---|
| Format: | Book |
| Language: | English |
| Published: |
Boston [u.a.]
Acad. Press
1993
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| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Physical Description: | XV, 312 S. zahlr. graph. Darst. |
| ISBN: | 0127423559 |
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Record in the Search Index
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| adam_text | VRACTICAL CAPILLARY ELECTROPHORESIS ROBERT WEINBERGER CE TECHNOLOGIES
CHAPPAQUA, NEW YORK ACADEMIC PRESS, INC. HARCOURT BRUCE JOVANOVICH,
PUBLISHERS BOSTON SAN DIEGO NEW YORK LONDON SYDNEY TOKYO TORONTO
CONTENTS PREJACE MASTER SYMBOL LIST 1.1 1.2 1.3 1.4 1.5 1.6 1.7 2.1 2.2
2.3 2.4 2.5 2.6 2.7 2.8 2.9 2.10 2.11 1. INTRODUCTION ELECTROPHORESIS
MICROCHROMATOGRAPHIC SEPARATION METHODS CAPILLARY ELECTROPHORESIS
HISTORICAL PERSPECTIVE INSTRUMENTATION SOURCES OF INFORMATION ON HPCE
CAPILLARY ELECTROPHORESIS*A FAMILY OF TECHNIQUES REFERENCES 2. BASIC
CONCEPTS ELECTRICAL CONDUCTION IN FLUID SOLUTION THE LANGUAGE OF
ELECTROPHORESIS ELECTROENDOOSMOSIS EFFICIENCY RESOLUTION JOULE HEATING
OPTIMIZING THE VOLTAGE AND TEMPERATURE CAPILLARY DIAMETER AND BUFFER
IONIC STRENGTH OPTIMIZING THE CAPILLARY LENGTH BUFFERS CAPILLARIES
REFERENCES XI XIII 1 1 5 6 10 12 14 14 14 17 17 19 20 26 28 29 33 35 37
37 40 42 CONTENTS 3. CAPILLARY ZONE ELECTROPHORESIS 45 3.1 INTRODUCTION
45 3.2 MOBILITY 46 3.3 BUFFER ADDITIVES 50 3.4 SOLUTE-WALL INTERACTIONS
52 3.5 BANDBROADENING 60 3.6 APPLICATIONS 62 3.7 METHODS DEVELOPMENT 72
REFERENCES 75 4. CAPILLARY ISOELECTRIC FOCUSING 81 4.1 INTRODUCTION 81
4.2 CAPILLARY COATINGS AND ELECTROOSMOTIC ROW 85 4.3 PERFORMING A RUN 86
4.4 FOCUSING AND MOBILIZATION PROBLEMS 92 4.5 APPLICATIONS 93 REFERENCES
97 5. SIZE SEPARATIONS IN CAPILLARY GELS AND POLYMER NETWORKS 99 5.1
INTRODUCTION 99 5.2 SEPARATION MECHANISM 100 5.3 MATERIALS FORCGE 101
5.4 APPLICATIONS 118 5.5 PULSED-FIELD CAPILLARY ELECTROPHORESIS 125 5.6
THE FUTURE OF CGE 126 REFERENCES 127 6. CAPILLARY ISOTACHOPHORESIS 131
6.1 INTRODUCTION 131 6.2 SEPARATION MECHANISM 131 6.3 INSTRUMENTAL
ASPECTS 133 6.4 BUFFER SELECTION 136 6.5 ADVANTAGES AND PROBLEMS WITH
CITP 138 6.6 APPLICATIONS 140 REFERENCES 144 7. ELECTROKINETIC CAPILLARY
CHROMATOGRAPHY 147 7.1 INTRODUCTION 147 7.2 MICELLES 148 7.3 SEPARATION
MECHANISM 151 7.4 SOURCES OF BANDBROADENING 153 CONTENTS IX 7.5
SELECTING THE ELECTROLYTE SYSTEM 157 7.6 ELUTION RANGE OF MECC 160 7.7
ALTERNATIVE SURFACTANT SYSTEMS 163 7.8 APPLICATIONS AND METHODS
DEVELOPMENT 167 7.9 CYCLODEXTRINS 171 7.10 CHIRAL RECOGNITION 176 7.11
OPTIMIZATION 184 REFERENCES 186 8. CAPILLARY ELECTROCHROMATOGRAPHY 189
8.1 THE ELECTROOSMOTIC PUMP 189 8.2 BANDBROADENING 191 8.3 APPLICATIONS
193 8.4 ELECTROCHROMATOGRAPHY IN PERSPECTIVE 194 REFERENCES 196 9.
INJECTION 197 9.1 VOLUMETRIE CONSTRAINTS ON INJECTION SIZE 197 9.2
PERFORMING AN INJECTION AND RUN 199 9.3 INJECTION TECHNIQUES 199 9.4
INJECTION PROBLEMS AND SOLUTIONS 204 9.5 ALTERNATIVE INJECTION SCHEMES
205 9.6 STACKING 205 9.7 OTHER ENRICHMENT TECHNIQUES 218 REFERENCES 221
10. DETECTION 223 10.1 ON-CAPILLARY DETECTION 223 10.2 THE DETECTION
PROBLEM 225 10.3 CLASSES OF DETECTORS 225 10.4 LIMITS OF DETECTION 227
10.5 BANDBROADENING 228 10.6 ABSORPTION DETECTION 229 10.7 FLUORESCENCE
DETECTION 234 10.8 INDIRECT DETECTION 243 10.9 MASS SPECTROMETRY 246
10.10 OTHER DETECTION TECHNIQUES 256 10.11 FRACTION COLLECTION 258
REFERENCES 262 11. PUTTING IT ALL TOGETHER 267 11.1 SELECTING THE MODE
OF HPCE 267 X CONTENTS 11.2 REQUIREMENTS FOR ROBUST SEPARATIONS 11.3
REALISTIC COMPROMISES 11.4 LINEAR DYNAMIC RANGE 11.5 SAMPLE PREPARATION
11.6 QUANTITATIVE ANALYSIS 11.7 CORRECTION FACTORS REFERENCES 12.
SPECIAL TOPICS 12.1 INTRODUCTION 12.2 FIELD EFFECT ELECTROENDOOSMOSIS
12.3 ULTRAFAST SEPARATIONS 12.4 HPCEONACHIP 12.5 MULTIPLE CAPILLARY
SYSTEMS 12.6 GRADIENT AND PROGRAMMED SYSTEMS 12.7 TWO-DIMENSIONAL
SYSTEMS 12.8 IMMUNOLOGIE TRACE ENRICHMENT REFERENCES INDEX 268 269 269
273 280 283 284 287 287 287 291 295 297 299 303 303 305 307
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| indexdate | 2024-07-09T17:17:03Z |
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| isbn | 0127423559 |
| language | English |
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| spelling | Weinberger, Robert Verfasser aut Practical capillary electrophoresis Robert Weinberger Boston [u.a.] Acad. Press 1993 XV, 312 S. zahlr. graph. Darst. txt rdacontent n rdamedia nc rdacarrier Kapillarelektrophorese (DE-588)4290002-5 gnd rswk-swf Kapillarelektrophorese (DE-588)4290002-5 s DE-604 GBV Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=005445637&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Weinberger, Robert Practical capillary electrophoresis Kapillarelektrophorese (DE-588)4290002-5 gnd |
| subject_GND | (DE-588)4290002-5 |
| title | Practical capillary electrophoresis |
| title_auth | Practical capillary electrophoresis |
| title_exact_search | Practical capillary electrophoresis |
| title_full | Practical capillary electrophoresis Robert Weinberger |
| title_fullStr | Practical capillary electrophoresis Robert Weinberger |
| title_full_unstemmed | Practical capillary electrophoresis Robert Weinberger |
| title_short | Practical capillary electrophoresis |
| title_sort | practical capillary electrophoresis |
| topic | Kapillarelektrophorese (DE-588)4290002-5 gnd |
| topic_facet | Kapillarelektrophorese |
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