Working with animal chromosomes:
Saved in:
| Main Authors: | , |
|---|---|
| Format: | Book |
| Language: | English |
| Published: |
Chichester u.a.
Wiley
1988
|
| Edition: | 2. ed. |
| Series: | A Wiley-Interscience publication
|
| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Physical Description: | XV, 290 S. Ill., graph. Darst. |
| ISBN: | 0471920282 |
Staff View
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| 100 | 1 | |a Macgregor, Herbert C. |e Verfasser |4 aut | |
| 245 | 1 | 0 | |a Working with animal chromosomes |c Herbert C. Macgregor ; Jennifer M. Varley |
| 250 | |a 2. ed. | ||
| 264 | 1 | |a Chichester u.a. |b Wiley |c 1988 | |
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| 650 | 4 | |a Animals - Cells - Chromosomes - Analysis - Laboratory techniques | |
| 650 | 4 | |a Chromosomes | |
| 650 | 4 | |a Génétique animale - Technique | |
| 650 | 4 | |a Animal genetics |x Technique | |
| 650 | 4 | |a Chromosomes | |
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Record in the Search Index
| _version_ | 1804122488441929728 |
|---|---|
| adam_text | IMAGE 1
WORKING WITH
ANIMAL CHROMOSOMES SECOND EDITION HERBERT C. MACGREGOR DEPARTMENT OF
ZOOLOGY, UNIVERSITY OF LEICESTER, LEICESTER, UK
AND
JENNIFER M. VARLEY ICI/UNIVERSITY JOINT LABORATORY,
DEPARTMENT OF BIOCHEMISTRY, UNIVERSITY OF LEICESTER, LEICESTER, UK
CHAPTER 3, SECTION VI CONTRIBUTED BY GARETH JONES DEPARTMENT OF
GENETICS, UNIVERSITY OF BIRMINGHAM, UK
CHAPTER 4, SECTIONS V.D, VI AND IX CONTRIBUTED BY THOMAS HAAF AND
MICHAEL SCHMID DEPARTMENT OF HUMAN GENETICS, UNIVERSITY OF WIIRZBURG,
FRG
CHAPTER 5, SECTION VII CONTRIBUTED BY CLAUS PELLING MAX PLANCK INSTITUT
FIIR BIOLOGIE, TUBINGEN, FRG
CHAPTER 7 CONTRIBUTED BY AIMEE BAKKEN
DEPARTMENT OF ZOOLOGY, UNIVERSITY OF WASHINGTON, SEATTLE, WASHINGTON,
USA, AND
R. STEPHEN HILL
DEPARTMENT OF GENETICS, UNIVERSITY OF LIVERPOOL, UK
A WILEY-INTERSCIENCE PUBLICATION
JOHN WILEY & SONS CHICHESTER * NEW YORK * BRISBANE * TORONTO * SINGAPORE
IMAGE 2
CONTENTS
PREFACE TO SECOND EDITION XIII
CHAPTER 1 CHROMOSOME SIZE, NUMBER AND SHAPE 1
REFERENCES 10
CHAPTER 2 MITOTIC CHROMOSOMES 11
I. PRINCIPLES OF THE SPLASH TECHNIQUE 13
II. PREPARATION OF HUMAN CHROMOSOMES FROM LYMPHOCYTES USING THE SPLASH
TECHNIQUE 17
A. EQUIPMENT AND MATERIALS 17
B. PROCEDURE 17
III. VARIATIONS ON THE HUMAN LYMPHOCYTE TECHNIQUE 21
A. ADJUSTMENTS TO THE PREPARATION OF SLIDES 21
B. OTHER MAMMALIAN SPECIES AND OTHER TISSUE TYPES 22
C. ALL MITOTIC STAGES 23
IV. NON-MAMMALIAN SOURCES 24
A. AVIAN LYMPHOCYTES 24
B. SNAKE LYMPHOCYTES 26
C. AMPHIBIAN INTESTINAL EPITHELIAL CELLS 26
D. AMPHIBIAN BONE MARROW CELLS 27
V. MITOTIC CHROMOSOMES USING THE SQUASH TECHNIQUE 29
A. AMPHIBIAN INTESTINAB EPITHELIAL TISSUE 30
B. AVIAN FEATHER PULP 31
C. NEUROBLASTS OF DIPTERAN LARVAE 32
D. LOCUST EMBRYOS 33
VI. MAKING PERMANENT PREPARATIONS 33
REFERENCES . 34
VII
IMAGE 3
VIII CONTENTS
CHAPTER 3 CHROMOSOMES IN MEIOSIS 37
I. CHOICE OF ANIMALS 42
A. SALAMANDERS 42
B. LOCUSTS AND GRASSHOPPERS 44
C. MICE AND OTHER MAMMALS 46
II. GENERAL EQUIPMENT NEEDED 47
III. FEULGEN-STAINED SQUASHES OF AMPHIBIAN TESTIS 48
A. PROCEDURE 49
B. PERMANENT PREPARATIONS WITH NO FREEZING 53
C. LOOKING AT SQUASHES OF AMPHIBIAN TESTIS 53
IV. ORCEIN-STAINED SQUASHES OF LOCUST OR GRASSHOPPER TESTIS 54
A. PROCEDURE 56
B. LOOKING AT SQUASHES OF LOCUST OR GRASSHOPPER TESTIS 58
V. AIR-DRIED MEIOTIC CHROMOSOMES FROM MICE 61
A. PROCEDURE 62
B. LOOKING AT MOUSE CHROMOSOMES 64
VI. SURFACE-SPREADING OF SYNAPTONEMAL COMPLEXES 65
A. MATERIALS AND EQUIPMENT NEEDED 67
B. PROCEDURE 68
C. LOOKING AT SURFACE-SPREAD SYNAPTONEMAL COMPLEXES 70 REFERENCES 70
CHAPTER 4 CHROMOSOME BANDING 73
I. GIEMSA STAINING 76
II. GIEMSA C-BANDING 77
A. C-BANDING AFTER BARIUM HYDROXIDE 78
III. GIEMSA G-BANDING 80
A. TRYPSIN G-BANDING 81
B. ACETIC ACID-SALINE-GIEMSA (ASG) BANDING 83
IV. QUINACRINE (Q)-BANDING 83
A. THE CASPERSSON METHOD 84
B. Q-BANDING USING QUINACRINE DIHYDROCHLORIDE 84
V. R-BANDING 85
A. R-BANDMG BY HIGH-TEMPERATURE TREATMENT 86
B. R-BANDING BY LOW-PH TREATMENT 86
C. R-BANDING WITH ACRIDINE ORANGE 86
D. R-BANDING USING CHROMOMYCIN A3 87
E. TELOMERIC R-BANDING 87
, VI. DISTAMYCIN A-DAPI BANDING 88
IMAGE 4
CONTENTS IX
VII. SILVER STAINING 90
A. AG-AS STAINING 90
B. A SIMPLIFIED METHOD 92
VIII. DEMONSTRATION OF SISTER CHROMATID EXCHANGE 93
A. THE FLUOROCHROME PLUS GIEMSA (FPG) METHOD 93
B. DEMONSTRATION OF REPLICATION PATTERNS WITH BRDU 95
IX. INDIRECT IMMUNOFLUORESCENCE 97
A. CHROMOSOME PREPARATION WITHOUT ACID FIXATION 97
B. IMMUNOFLUORESCENT STAINING 99
C. MEIOTIC CELLS AND CHROMOSOMES 102
D. ACID-FAST ANTIGENS 103
E. GIANT CHROMOSOMES 104
F. ANTIBODIES 105
G. IMPORTANT NOTES 106
REFERENCES 107
ADDITIONAL REFERENCES NOT CITED IN TEXT 110
CHAPTER 5 POLYTENE CHROMOSOMES 115
I. PRINCIPLES OF TECHNIQUES FOR POLYTENE CHROMOSOMES 117
II. MATERIALS AND EQUIPMENT NEEDED 118
III. POLYTENE CHROMOSOMES FROM CHIRONOMUS SALIVARY GLANDS 119 A.
PROCEDURE 120
B. A QUICK METHOD FOR CHIRONOMUS 122
C. POLYTENE CHROMOSOMES BY NOMARSKI MICROSCOPY 123
IV. POLYTENE CHROMOSOMES FROM DROSOPHILA 124
A. MAINTENANCE OF STOCKS 124
B. PROCEDURE 125
V. POLYTENE CHROMOSOMES FOR LOOKING AT CHROMOSOMAL PROTEIN 129 A.
PROCEDURE 130
VI. POLYTENE CHROMOSOMES FROM THE NURSE CELLS OF MOSQUITOES 131 A.
MAINTENANCE OF STOCKS 132
B. PROCEDURE 133
VII. ISOLATION OF UNFIXED POLYTENE CHROMOSOMES 135
A. STANDARD METHOD 135
B. MODIFIED METHOD FOR CLEANER CHROMOSOMES 138
C. GENERAL COMMENTS 139
REFERENCES 141
EFERENCES TO POLYTENE CHROMOSOMES MAPS OF SOME WIDELY STUDIED DROSOPHILA
AND CHIRONOMUS SPECIES 143
IMAGE 5
X CONTENTS
CHAPTER 6 LAMPBRUSH CHROMOSOMES 145
I. CHOICE OF ANIMALS 147
A. NOTES ON INDIVIDUAL SPECIES 148
II. MAINTENANCE OF ANIMALS 150
III. OBTAINING OOCYTES AND CHROMOSOMES 150
A. OBTAINING OOCYTES BY LAPAROTOMY WITH ANAESTHESIA 150 B. MAKING
LAMPBRUSH PREPARATIONS 152
C. LOOKING AT FRESHLY ISOLATED LAMPBRUSHES 162
D. IDENTIFICATION AND MAPPING OF CHROMOSOMES 164
E. PERMANENT PREPARATIONS 168
IV. MODIFICATIONS OF THE BASIC TECHNIQUE FOR SPECIAL PURPOSES 173 A. THE
ACTIONS OF ENZYMES ON LAMPBRUSH CHROMOSOMES 173 B. RADIOISOTOPE
INCORPORATION INTO GERMINAL VESICLES 173 C. IMMUNOFLUORESCENT STAINING
OF LAMPBRUSH CHROMOSOMES 176 V. STAINING LAMPBRUSH CHROMOSOMES 176
VI. LAMPBRUSH CHROMOSOMES FROM ANIMALS OTHER THAN NEWTS 177 VII.
LAMPBRUSH CHROMOSOMES FROM XENOPUS LAEVIS 178
A. THE ANIMALS 179
B. THE MEDIA 179
C. THE OBSERVATION CHAMBERS 180
D. THE OOCYTES 180
REFERENCES 180
CHAPTER 7 VISUALIZATION OF TRANSCRIPTION 183
(CONTRIBUTED BY AIMEE H. BAKKEN AND R. S. HILL)
I. VISUALIZING TRANSCRIPTION IN OOCYTES 188
A. NUCLEOLAR ( = RIBOSOMAL) DNA AND T
LAMPBRUSH CHROMOSOMES 1883
B. ALTERNATIVE METHODS FOR LAMPBRUSH CHROMOSOMES 191
II. ELECTRON MICROSCOPE IMAGES OF TRANSCRIPTION IN OOCYTES 192 III.
RATIONALE AND COMMENTS ON METHODS FOR GERMINAL VESICLES 194 IV.
MODIFICATION OF THE TECHNIQUE FOR OTHER CELL TYPES 198
A. EMBRYONIC CELLS 199
B. TISSUE TULTURE CELLS 200
C. TRANSCRIPTION OF DNA INJECTED INTO OOCYTE NUCLEI 201
V. SOLUTIONS AND EQUIPMENT FOR MILLER SPREADING 202
VI. ANALYSING CHROMATIN SPREADS AND TRANSCRIPTION UNITS 207
VII. CONCLUDING REMARKS 210
.REFERENCES 211
IMAGE 6
CONTENTS XI
CHAPTER 8 IN SITU HYBRIDIZATION 215
I. PROBE PREPARATION 217
A. CHOICE OF PROBE-DNA OR RNA? 217
B. CHOICE OF LABEL 220
C. PRODUCTION OF LABELLED DNA BY NICK TRANSLATION 221
D. PRODUCTION OF LABELLED DNA USING A RANDOM PRIMING METHOD 223
E. PRODUCTION OF LABELLED CRNA USING E. COLI RNA POLYMERASE 224
F. SYNTHESIS OF LABELLED RNA USING OTHER POLYMERASES 226 II.
HYBRIDIZATION TO CHROMOSOMAL STRUCTURES 230
A. GENERAL POINTS 230
B. HYBRIDIZATION TO REPETITIVE CHROMOSOMAL DNA SEQUENCES 233
C. HYBRIDIZATION TO SINGLE-COPY DNA SEQUENCES ON CHROMOSOMES 236
D. HYBRIDIZATION TO NASCENT CHROMOSOMAL RNA TRANSCRIPTS 237
III. HYBRIDIZATION TO RNA TRANSCRIPTS IN TISSUE SECTIONS 238
A. TISSUE PREPARATION - FIXATION AND SECTIONING 239
B. PRETREATMENT 240
C. PROBE PREPARATION 241
D. HYBRIDIZATION, WASHING AND AUTORADIOGRAPHY 242
REFERENCES 245
:HAPTER 9 AUTORADIOGRAPHY 247
I.
II. III. IV. V.
VI.
VII. III. IX. X.
AUTORADIOGRAPHIC EMULSIONS DARKROOM THE DIPPING CHAMBER SLIDE STORAGE
BOXES FOR AUTORADIOGRAPHS SUBBED SLIDES
CHECKLIST OF EQUIPMENT AND PROTOCOL FOR PREPARING AUTORADIOGR&PHS
EXPOSURE
DEVELOPMENT REMOVAL OF AUTORADIOGRAPHIC BACKGROUND WITH PEROXIDE
STAINING OF AUTORADIOGRAPHS DDITIONAL SOURCES OF INFORMATION
248 252 252 253
253
253 255 255 257
257 258
IMAGE 7
XII CONTENTS
CHAPTER 10 MEASURING NUCLEAR OR CHROMOSOMAL DNA 259
I. MICRODENSITOMETRY 261
II. THE FEULGEN REACTION 264
A. FIXATION 264
B. HYDROLYSIS 265
C. STAINING 265
D. WASHING 267
E. DEHYDRATION AND MOUNTING 267
F. MEASURING 268
G. CALIBRATING 269
REFERENCES 270
CHAPTER 11 SAFETY AND THE LAW 271
I. DANGEROUS CHEMICALS 271
II. RADIOISOTOPES 272
III. THE HANDLING OF HUMAN BLOOD AND TISSUE SAMPLES 272
IV. SCIENTIFIC PROCEDURES WITH LIVE ANIMALS 274
V. IMPORTATION, EXPORTATION AND PROCUREMENT FROM THE WILD OF LIVE
ANIMALS 275
CONCLUDING REMARK 275
REFERENCES 275
APPENDIX NAMES, ADDRESSES AND TELEPHONE NUMBERS OF SUPPLIERS 275
INDEX 283
|
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| author | Macgregor, Herbert C. Varley, Jennifer M. |
| author_facet | Macgregor, Herbert C. Varley, Jennifer M. |
| author_role | aut aut |
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| building | Verbundindex |
| bvnumber | BV008113915 |
| callnumber-first | Q - Science |
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| callnumber-raw | QH600 |
| callnumber-search | QH600 |
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| callnumber-subject | QH - Natural History and Biology |
| classification_rvk | WC 4400 WC 4440 WC 5100 |
| ctrlnum | (OCoLC)17953872 (DE-599)BVBBV008113915 |
| dewey-full | 591.87/322 |
| dewey-hundreds | 500 - Natural sciences and mathematics |
| dewey-ones | 591 - Specific topics in natural history of animals |
| dewey-raw | 591.87/322 |
| dewey-search | 591.87/322 |
| dewey-sort | 3591.87 3322 |
| dewey-tens | 590 - Animals |
| discipline | Biologie |
| edition | 2. ed. |
| format | Book |
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| id | DE-604.BV008113915 |
| illustrated | Illustrated |
| indexdate | 2024-07-09T17:14:40Z |
| institution | BVB |
| isbn | 0471920282 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-005346766 |
| oclc_num | 17953872 |
| open_access_boolean | |
| owner | DE-20 |
| owner_facet | DE-20 |
| physical | XV, 290 S. Ill., graph. Darst. |
| publishDate | 1988 |
| publishDateSearch | 1988 |
| publishDateSort | 1988 |
| publisher | Wiley |
| record_format | marc |
| series2 | A Wiley-Interscience publication |
| spelling | Macgregor, Herbert C. Verfasser aut Working with animal chromosomes Herbert C. Macgregor ; Jennifer M. Varley 2. ed. Chichester u.a. Wiley 1988 XV, 290 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier A Wiley-Interscience publication Animals - Cells - Chromosomes - Analysis - Laboratory techniques Chromosomes Génétique animale - Technique Animal genetics Technique Cytological Techniques Tiere (DE-588)4060087-7 gnd rswk-swf Methode (DE-588)4038971-6 gnd rswk-swf Chromosom (DE-588)4010162-9 gnd rswk-swf Chromosom (DE-588)4010162-9 s Tiere (DE-588)4060087-7 s Methode (DE-588)4038971-6 s DE-604 Varley, Jennifer M. Verfasser aut HEBIS Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=005346766&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Macgregor, Herbert C. Varley, Jennifer M. Working with animal chromosomes Animals - Cells - Chromosomes - Analysis - Laboratory techniques Chromosomes Génétique animale - Technique Animal genetics Technique Cytological Techniques Tiere (DE-588)4060087-7 gnd Methode (DE-588)4038971-6 gnd Chromosom (DE-588)4010162-9 gnd |
| subject_GND | (DE-588)4060087-7 (DE-588)4038971-6 (DE-588)4010162-9 |
| title | Working with animal chromosomes |
| title_auth | Working with animal chromosomes |
| title_exact_search | Working with animal chromosomes |
| title_full | Working with animal chromosomes Herbert C. Macgregor ; Jennifer M. Varley |
| title_fullStr | Working with animal chromosomes Herbert C. Macgregor ; Jennifer M. Varley |
| title_full_unstemmed | Working with animal chromosomes Herbert C. Macgregor ; Jennifer M. Varley |
| title_short | Working with animal chromosomes |
| title_sort | working with animal chromosomes |
| topic | Animals - Cells - Chromosomes - Analysis - Laboratory techniques Chromosomes Génétique animale - Technique Animal genetics Technique Cytological Techniques Tiere (DE-588)4060087-7 gnd Methode (DE-588)4038971-6 gnd Chromosom (DE-588)4010162-9 gnd |
| topic_facet | Animals - Cells - Chromosomes - Analysis - Laboratory techniques Chromosomes Génétique animale - Technique Animal genetics Technique Cytological Techniques Tiere Methode Chromosom |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=005346766&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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