Guide to flow cytometry methods:
Saved in:
| Main Authors: | , |
|---|---|
| Format: | Book |
| Language: | English |
| Published: |
New York u.a.
Dekker
1990
|
| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Physical Description: | X, 228 S. graph. Darst. |
| ISBN: | 0824783301 |
Staff View
MARC
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| 245 | 1 | 0 | |a Guide to flow cytometry methods |c W. McLean Grogan ; James M. Collins |
| 264 | 1 | |a New York u.a. |b Dekker |c 1990 | |
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Record in the Search Index
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| adam_text | Guide to Flow
Cytometry Methods
W McLean Grogan
James M Collins
Medical College of Virginia
Virginia Commonwealth University
Richmond, Virginia
Marcel Dekker, Inc New York and Basel
Contents
Preface
1 Introduction
Flow Cytometers
Characteristics of Light
Fluorescence
Optical Filters
Filter Selection Strategy for Analysis
Calibration of Flow Cytometers
Data Collection, Display and Selective Analysis
Safety in the Flow Cytometry Laboratory
References
Recommended Reading
2 Preparation of Single Cell Suspensions
Introduction
Cell Clumping
Monolayer Cell Cultures
Tissues
Methods
Method 2 1: Solid Tissue Dispersal
m
vi Contents
Method 2 2:
Method 2 3:
Method 2 4:
Method 2 5:
Method 2 6:
Method 2 7:
References
3 DNA Analysis
Blood Lymphocytes (General Method)
Preparation of Bone Marrow Cells
Lymphocyte Isolation by Ficoll Gradient
(General Method)
Liver Cells
Testis Cells
Head and Neck Tumor Cell Dispersal
3 1 Staining and Fixation
3 2 DNA Staining
3 3 Applications of DNA Content Analysis
(Noncell Cycle)
3 4 Cell Cycle of Normal Cells
3 5 Cell Cycle Staging with Thymidine Analogues
3 6 The Cell Cycle of Cancer Cells
3 7 Applications of DNA Analysis to the Clinical Management
of Cancer
3 8 Spermatogenesis
3 9 Calibration of DNA Distributions
Method 3 1: Propidium Iodide-Hypotonic Lysis
Method 3 2: Propidium-Iodide-Crissman (PIC) Procedure
Method 3 3: Modified Vindelov s Low Salt
Method 3 4: Modified Vindelov s High Salt Procedure (Fine
Needle Biopsy of Solid Tumor)
Method 3 5: Labeling Cells with Anti-Bromodeoxyuridine
and PI
Method 3 6: Bromodeoxyuridine—Improved Method
Method 3 7: Hoechst Dye for Staining of Viable Cells
Method 3 8: Simultaneous Differential Staining of DNA
and RNA in Situ with Acridine Orange
Method 3 9: Mithramycin Procedure
Method 3 10: Combined Ethidium Bromide—Mithramycin
Procedure
Method 3 11: Dewaxing of Paraffinized Material
References
Recommended Reading
4 Viability 81
Contents vii
4 1 Introduction
4 2 Use of Fluorescein Diacetate and Propidium Iodide
4 3 Other Methods
4 4 Specific Applications
Method 4 1: Fluorescein Diacetate (FDA) Viability
Staining
Method 4 2: Combined Fluorescein Diacetate and
Propidium Iodide
Method 4 3: Hoechst-Propidium Iodide for
Viability
References
5 Light Scatter (LS)
5 1 Introduction
5 2 Applications
5 3 Current Instrumentation
5 4 Theoretical Considerations
References
6 Immunofluorescence
6 1 Introduction 123
6 2 Analysis of Mixed Populations 123
6 3 Two-Color Analysis 124
6 4 Three-Color Immunofluorescence 128
6 5 Selected Applications 128
6 6 Lymphocyte Subset Analysis 128
6 7 Number of Antibody Binding Sites 130
Method 6 1: Paraformaldehyde Fixation of Immuno-
fluorescence Labeled Lymphocytes
for Flow Analysis 130
Method 6 2: Indirect Immunofluorescence of Blood
(Ortho Method) 131
Method 6 3: Direct Immunofluorescence of Blood
(Ortho Method) 132
Method 6 4: Indirect Immunofluorescence: Coulter
Clone Method 132
Method 6 5: Direct Immunofluorescence Coulter Clone 134
Method 6 6: Indirect Immunofluorescent Staining of
Immunoglobulin Sites on Human Lymphocytes
(General Method) 135
Method 6 7: Antigen-Binding B Cells (General Method) 135
viii Contents
References 136
7 Cell Sorting 138
7 1 Introduction 138
7 2 Mechanism 139
7 3 Coincidence and the Statistics of Sorting 143
7 4 Cell Collection 145
7 5 Applications 148
7 6 Future Developments 148
References 151
8 Mitochondrial Stains 154
8 1 Introduction 154
8 2 Mitochondrial Dyes 154
8 3 Applications 155
Method 8 1: Mitochondrial Staining with Rhodamine-123 155
References 158
9 Intracellular pH 159
9 1 Introduction 159
9 2 Applications Using Flow Cytometry 159
9 3 Flow Cytometric pH; Dyes 160
Method 9 1: pHj Calibration and pHj Measurement 161
Method 9 2: 4-Methylumbelliferone Staining for pH 162
References 163
10 Intracellular Calcium 164
10 1 Introduction 164
10 2 Calcium Dyes 165
10 3 Applications 167
Method 10 1: Changes in Calcium Levels by Indo-1 168
References 168
11 Glutathione 171
11 1 Introduction 171
11 2 Flow Cytometric Measurement 171
11 3 General Applications 174
Method 11 1: Intracellular Glutathione Using
Monochlorobimane 175
Contents ix
References 176
12 Fluorescence Anisotropy and Membrane Fluidity 177
12 1 Anisotropy 177
12 2 Membrane Fluidity 179
12 3 Membrane Probes 180
12 4 Membrane Fluidity of Intact Cells • 181
12 5 Flow Cytometry Applications to Membrane Fluidity 182
12 6 Measurement of Anisotropy 183
Method 12 1: Anisotropy Measurement for Membrane
Fluidity 187
References 187
13 Cell Surface Receptors 190
13 1 Introduction 190
13 2 Scatchard Theory • • • 190
13 3 Traditional Method 192
13 4 Fluorescence Methods 193
13 5 Static Fluorometry 194
13 6 Flow Cytometry 194
13 7 Heterogeneity of Receptor Density 198
13 8 Low Number Receptors 199
13 9 Applications 199
Method 13 1: Three Tube Method for Estimation of
Number of Receptors , 201
References 202
Recommended Reading 203
14 Cellular Membrane Potential 204
15 Miscellaneous Applications 211
Membranes
Membrane Permeability
Membrane Potential
Flow Cytometry
Applications
Method 14 1: Membrane Potential Changes
References
Drug Uptake
Phagocytosis
Contents
References 215
Appendix 217
A 1 Fluorescence Spectra of DNA Stains 217
A 2 Fluorescence Spectra of Immunofluorescence Stains 218
A 3 Fluorescence Spectra of Membrane Potential Dyes 218
A 4 Fluorescence Spectra of Membrane Anisotropy Dyes 218
A 5 Supplies 219
A 6 Flow Cytometry Software 223
Index 224
Fluid Pinocytosis
Fluorescent Derivatives
Oxidative Burst
Virus Adsorption
Chromosome Analysis
Slit-Scan Systems
Time of Flight
Enzyme Analysis
Platelets
Time as a Parameter
|
| any_adam_object | 1 |
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| dewey-sort | 3574.87 228 |
| dewey-tens | 570 - Biology |
| discipline | Biologie Medizin |
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| indexdate | 2024-07-09T17:08:18Z |
| institution | BVB |
| isbn | 0824783301 |
| language | English |
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| spelling | Grogan, W. M. Verfasser aut Guide to flow cytometry methods W. McLean Grogan ; James M. Collins New York u.a. Dekker 1990 X, 228 S. graph. Darst. txt rdacontent n rdamedia nc rdacarrier Flow Cytometry methods Flow cytometry Durchflusscytometrie (DE-588)4226628-2 gnd rswk-swf Durchflusscytometrie (DE-588)4226628-2 s DE-604 Collins, James M. Verfasser aut HEBIS Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=005072154&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Grogan, W. M. Collins, James M. Guide to flow cytometry methods Flow Cytometry methods Flow cytometry Durchflusscytometrie (DE-588)4226628-2 gnd |
| subject_GND | (DE-588)4226628-2 |
| title | Guide to flow cytometry methods |
| title_auth | Guide to flow cytometry methods |
| title_exact_search | Guide to flow cytometry methods |
| title_full | Guide to flow cytometry methods W. McLean Grogan ; James M. Collins |
| title_fullStr | Guide to flow cytometry methods W. McLean Grogan ; James M. Collins |
| title_full_unstemmed | Guide to flow cytometry methods W. McLean Grogan ; James M. Collins |
| title_short | Guide to flow cytometry methods |
| title_sort | guide to flow cytometry methods |
| topic | Flow Cytometry methods Flow cytometry Durchflusscytometrie (DE-588)4226628-2 gnd |
| topic_facet | Flow Cytometry methods Flow cytometry Durchflusscytometrie |
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