Bioluminescence analysis:
Gespeichert in:
| Hauptverfasser: | , |
|---|---|
| Format: | Buch |
| Sprache: | German |
| Veröffentlicht: |
Weinheim u.a.
VCH-Verl.-Ges.
1992
|
| Ausgabe: | 1. ed. |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | Literaturangaben |
| Beschreibung: | X, 151 S. Ill., graph. Darst. |
| ISBN: | 3527281940 089573995X |
Internformat
MARC
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| 100 | 1 | |a Brolin, Sven E. |e Verfasser |4 aut | |
| 245 | 1 | 0 | |a Bioluminescence analysis |c S. Brolin ; G. Wettermark |
| 250 | |a 1. ed. | ||
| 264 | 1 | |a Weinheim u.a. |b VCH-Verl.-Ges. |c 1992 | |
| 300 | |a X, 151 S. |b Ill., graph. Darst. | ||
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Datensatz im Suchindex
| _version_ | 1804122059472633856 |
|---|---|
| adam_text | S Brolin, G Wettermark
Bioluminescence Analysis
VCH Weinheim • New York • Basel • Cambridge
Table of Contents
1 The Phenomenon of Chemiluminescence 1
1 1 The Nature of Chemiluminescence 1
111 The Concept 1
112 The Excitation 2
113 Emission and Quenching 3
114 The Photon Emitter 4
115 Quantum Yield 6
1 2 Chemiluminescent Reactions 6
121 Discovery of Luminescent Compounds 6
122 Luminol 7
123A Wide Variety of Chemiluminescent Systems • 8
124 Common Reaction Mechanisms 10
125 Electrochemiluminescence 11
1 3 Bioluminescence Mechanisms 12
131 Common Features 12
132 Firefly 12
133 Photobacteria 13
1 4 Analytical Applications 14
141 The Growth of Chemiluminescence Analysis 14
142 Introduction of Bioluminescence 15
2 Principles of Analytical Applications of Luminescence 19
2 1 Compounds Which Can Be Analyzed 19
2 2 Kinetic Considerations 20
2 3 Characteristics of the Light Emission 21
2 4 Assay Based on Products of a Preceding Reaction 24
3 Enzyme Kinetics 25
3 1 Particular Considerations of Enzyme Kinetics 25
311 Emission and Reaction Rate 25
312 The Michaelis-Menten Relation 25
313 Inhibition of the Enzyme Reaction 26
314 Enzyme Activities and Rate Constants 27
3 2 Selecting Analytical Parameter when Determining a Light-Inducing
Compound 27
321 Measuring the Intensity Level or Integrating the Curve of the Emitted
Light 27
322 Rate Requirements for Adequate Sample Handling 28
323 Representation of the Start-Up Phase 30
VIII Table of Contents
4 Coupling of Reactions 33
4 1 Principal Approach 33
4 2 Non-Cycling Coupled Reactions in Substrate Analysis 34
421 General Behavior 34
422 Defining a Rate-Determining Reaction 36
423 Analysis Using Initial Rates 38
4 3 Cycling Reactions in Substrate Analysis 38
431 The Steady-State 38
432 Cycling Time and Amplification 41
433 Comparing Constant Light Producing Methods 42
4 4 Kinetics of Coupled Reactions in Enzyme Analysis 43
441 General Application 43
442 Determination from Initial Slope 44
443 Determination From Steady-State 45
5 Experimental Design 47
5 1 Selecting Appropriate Analytical Systems 47
5 2 The Firefly Luciferase System 47
521 Data Pertaining to the Planning of Assays 47
522 Time Profile of the Light Pulse 49
523 Nearly-Constant Light Emission 53
524 Assay of Phosphotransferases 53
525 Substrate Analysis 55
5 3 The Bacterial Luciferase System 56
531 The Two Enzymes 56
532 Emission Profiles of NADH and NADPH 58
533 Applying the Kinetics of Coupled Reactions 60
534 Substrate Analyses Through Direct Coupling 62
535 Assay of Enzymes with Direct Coupling 65
6 Preparation of Samples 69
6 1 Requirements 69
6 2 Preserving Species Subject to Assay 69
621 Arresting the Metabolie Processes 69
622 Lyophilization and Cryostat Techniques 72
623 Checking Alterations During Intermissions and Storage 72
624 Adjustment of Concentrations 73
6 3 Quantification of Samples 74
6 4 Planning the Initial Preparative Steps 76
641 Sampling from Cell Suspensions, Cultures of Cells and Small Organs 76
642 Sampling from Biopsies and Pieces of Organs 78
6 5 Extraction Methods 79
651 General Procedures 79
652 Adenine Nucleotides 80
Table of contents IX
653 Pyridine Nucleotides 82
654 Intermediary Metabolites 83
655 Enzymes 84
7 Generation and Measurement of Light Signals 87
7 1 Paths Towards Analytical Response 87
7 2 Desired Emission by Kinetic Means 88
721 Flash Techniques 88
722 Delayed Appearance of the Emission Maximum 88
723 Long-Lasting Emission 89
724 Stable Emission 89
7 3 The Light Measuring Procedure 9 1
731 Light Recording in Photometric Methods 91
732 Key Parameters Guiding the Design of Luminometers 92
7 4 Instrumental Facilities for Appropriate Recording 93
741 Sample Compartment and Light Collection 93
742 Solid-State and Vacuum Cells 95
743 Quantum Efficiency and Sensitivity of the Light Detector 96
744 Amplification of Light Detector Signals 98
745 Commercial Instruments 100
8 Fields of Application 103
81A Biological Outlook on Analytical Applicability 103
8 2 Biomass Determinations in Microbiology and Cell Research 104
821 Means of Quantification 104
822 Use of ATP 104
823 The Adenylate Pool (ATP+ADP+AMP) 105
824 The Concept of Biomass 108
825 Various Methods Compared 110
8 3 Clinical Chemistry 111
831 Applications in Established Routines 111
832 Immunochemistry 112
833 Biopsies 112
834 Cell and Organ Cultures 114
8 4 Preparative Biochemistry 114
8 5 Histochemistry 115
851 Visualization and Electronic Imaging 115
852 Microanalysis 115
9 Samples of Application 123
9 1 Cell Cultures 123
9 2 Single Cell Assay 123
921 General Aspects 123
922 Determination of the Cell Mass 124
Table of contents
923 Absorption Photometric and Fluorometric Measurements 124
924 Monitoring Discharge and Intracellular Events 124
925 Analysis of Cellular Extracts 125
9 3 Organ and Tissue Culture 126
931 Demands on Specimens 126
932 Suitable Specimens, Examples and Suggestions 127
9 4 Experimental Embryology 128
941 Initial Stages of Development 128
942 The Cultured Embryo as an Experimental Specimen 128
9 5 Laminar Elution 129
951 Selecting Preparative Procedures 129
952 Performance and Necessary Precautions 130
953 Choice of Membrane Destroyer 131
954 Modified Applications 133
9 6 Bioenergetic Evaluations 134
961 Metabolie Energy Provision Coupled to Light Formation 134
962 Evaluations Through Arrested Energy Flux 134
963 Inherent Weak Light Emission 137
9 7 Metabolie Transfer of the Sulfate Group 137
971 Metabolie Detoxification by Sulfatation 137
972 Analyses Based on the Renilla System 138
973 Analysis Based on the Firefly System 138
974 Aspects of Future Research 138
9 8 Discharge from Cells 139
981 Origin and Elimination of Extracellular Material 139
982 Monitoring the Discharge 139
983 Determination of the Physiological Discharge 141
9 9 Bioluminescence Probing 141
991 Cellular Components Employed as Labels 141
992 Intracellular Deposition of Markers 142
Index 147
|
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| author_facet | Brolin, Sven E. Wettermark, Gunnar |
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| author_sort | Brolin, Sven E. |
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| building | Verbundindex |
| bvnumber | BV007697679 |
| classification_rvk | WC 2800 WD 2800 |
| classification_tum | CHE 808f CHE 243f |
| ctrlnum | (OCoLC)246588031 (DE-599)BVBBV007697679 |
| discipline | Biologie Chemie |
| edition | 1. ed. |
| format | Book |
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| illustrated | Illustrated |
| indexdate | 2024-07-09T17:07:51Z |
| institution | BVB |
| isbn | 3527281940 089573995X |
| language | German |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-005053762 |
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| physical | X, 151 S. Ill., graph. Darst. |
| publishDate | 1992 |
| publishDateSearch | 1992 |
| publishDateSort | 1992 |
| publisher | VCH-Verl.-Ges. |
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| spelling | Brolin, Sven E. Verfasser aut Bioluminescence analysis S. Brolin ; G. Wettermark 1. ed. Weinheim u.a. VCH-Verl.-Ges. 1992 X, 151 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Literaturangaben Analyse (DE-588)4122795-5 gnd rswk-swf Biochemie (DE-588)4006777-4 gnd rswk-swf Biolumineszenz (DE-588)4145636-1 gnd rswk-swf Chemilumineszenz (DE-588)4147623-2 gnd rswk-swf Biomolekül (DE-588)4135124-1 gnd rswk-swf Biolumineszenz (DE-588)4145636-1 s Biochemie (DE-588)4006777-4 s DE-604 Biomolekül (DE-588)4135124-1 s Chemilumineszenz (DE-588)4147623-2 s Analyse (DE-588)4122795-5 s DE-188 Wettermark, Gunnar Verfasser aut HEBIS Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=005053762&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Brolin, Sven E. Wettermark, Gunnar Bioluminescence analysis Analyse (DE-588)4122795-5 gnd Biochemie (DE-588)4006777-4 gnd Biolumineszenz (DE-588)4145636-1 gnd Chemilumineszenz (DE-588)4147623-2 gnd Biomolekül (DE-588)4135124-1 gnd |
| subject_GND | (DE-588)4122795-5 (DE-588)4006777-4 (DE-588)4145636-1 (DE-588)4147623-2 (DE-588)4135124-1 |
| title | Bioluminescence analysis |
| title_auth | Bioluminescence analysis |
| title_exact_search | Bioluminescence analysis |
| title_full | Bioluminescence analysis S. Brolin ; G. Wettermark |
| title_fullStr | Bioluminescence analysis S. Brolin ; G. Wettermark |
| title_full_unstemmed | Bioluminescence analysis S. Brolin ; G. Wettermark |
| title_short | Bioluminescence analysis |
| title_sort | bioluminescence analysis |
| topic | Analyse (DE-588)4122795-5 gnd Biochemie (DE-588)4006777-4 gnd Biolumineszenz (DE-588)4145636-1 gnd Chemilumineszenz (DE-588)4147623-2 gnd Biomolekül (DE-588)4135124-1 gnd |
| topic_facet | Analyse Biochemie Biolumineszenz Chemilumineszenz Biomolekül |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=005053762&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT brolinsvene bioluminescenceanalysis AT wettermarkgunnar bioluminescenceanalysis |