Genetic engineering cloning DNA:
Saved in:
| Main Author: | |
|---|---|
| Format: | Book |
| Language: | English |
| Published: |
London
Chapman & Hall
1980
|
| Series: | Outline studies in biology.
|
| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Physical Description: | 78 S.: Ill. |
| ISBN: | 0412161702 |
Staff View
MARC
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Record in the Search Index
| _version_ | 1804120154996473856 |
|---|---|
| adam_text | IMAGE 1
CONTENTS
1 INTRODUCTORY REMARKS 7
REFERENCES 9
2 THE ENZYMOLOGY OF IN VITRO DNA RECOMBINATION 9
2.1 RESTRICTION ENDONUCLEASES 9
2.2 JOINING RESTRICTION FRAGMENTS WITH DNA LIGASE 10
2.3 JOINING DNA VIA HOMOPOLYMERIC TAILS 15
REFERENCES 17
3 PLASMID VECTORS 18
3.1 PSCLOL 18
3.2 COLEL 19
3.3 DERIVATIVES OF COLEL WHICH CONTAIN DRUG-RESISTANCE MARKERS 20 3.4
THE BIOLOGICAL CONTAINMENT OF A PLASMID VECTOR SYSTEM 24 3.5 SELECTION
OFPLASMIDS CONTAINING SPECIFIC NUCLEOTIDE SEQUENCES 24 REFERENCES 25
4 BACTERIOPHAGE^ VECTORS 26
4.1 THE BIOLOGY OF PHAGE A 26
4.2 PHAGE VECTORS 28
4.3 THE LATE GENES-THEIR EXPLOITATION IN CLONING VECTORS 32 4.3.1 PHAGE
ASSEMBLY 32
4.3.2 BIOLOGICAL CONTAINMENT 33
4.3.3 INCREASED RECOVERY OF RECOMBINANTS BY IN VITRO PACKAGING 34
REFERENCES 35
5 EXPRESSION OF CLONED DNAS IN E, COLI 36
5.1 EXPRESSION OF DNA CLONED IN PLASMID VECTORS 36
5.1.1 COMPLEMENTATION OF E. COLI AUXOTROPHS 36
5.1.2 ASSAYS FOR EXPRESSION OF NOVEL POLYPEPTIDES 37
5.1.3 EXPRESSION OF VERTEBRATE GENES IN E. COLI 39
5.2 EXPRESSION FROM PHAGE X PROMOTERS 47
REFERENCES 47
6 METHODS FOR THE PHYSICAL CHARACTERISATION OF CLONED SEGMENTS OF
CHROMOSOMAL DNA FROM HIGHER EUKARYOTES 48
6.1 MAPPING CLONED DNAS TO THEIR CHROMOSOMAL ORIGINS 49 6.1.1 IN SITU
HYBRIDISATION 49
6.1.2 SOMATIC CELL HYBRIDS 51
IMAGE 2
6.2 ELECTROPHORETIC MAPPING TECHNIQUES 52
6.2.1 RESTRICTION MAPPING 52
6.2.2 GEL TRANSFER HYBRIDISATION 54
6.2.3 MAPPING TRANSCRIPTS 57
6.3 MAPPING CLONED DNAS BY ELECTRON MICROSCOPY 58
6.3.1 DENATURATION MAPPING 59
6.3.2 HETERODUPLEX MAPPING 60
6.3.3 MAPPING REGIONS HOMOLOGOUS TO RNA 60
REFERENCES 65
7 APPROACHES FOR STUDYING EXPRESSION IN EUKARYOTIC SYSTEMS 66 7.1 IN
VITRO MUTAGENESIS 67
7.2 EXPRESSION SYSTEMS 68
7.2.1 CLONING IN YEAST 68
7.2.2 SV40 AS A CLONING VEHICLE 71
7.2.3 DIRECT TRANSFORMATION OF MAMMALIAN CELLS 74
7.2.4 MICROINJECTION OF CLONED DNAS INTO XENOPUS LAEVIS OOCYTES 75
REFERENCES 77
INDEX 79
ABBREVIATIONS
A P R AMPICILLIN RESISTANT
A P S AMPICILLIN SENSITIVE
CM 1 CHLORAMPHENICOL RESISTANT
C M S CHLORAMPHENICOL SENSITIVE KB KILOBASES - 1000 BASES OR BASE PAIRS
OF SINGLE OR DOUBLE
STRANDED NUCLEIC ACIDS RESPECTIVELY. P L LEFTWARD PROMOTER OF PHAGE X
P R RIGHTWARD PROMOTER OF PHAGE K
P R LATE RIGHTWARD PROMOTER OF PHAGE X
PRE PHAGE X PROMOTER FOR THE ESTABLISHMENT OF LYSOGENY PRM PHAGE X
PROMOTER FOR THE MAINTENANCE OF LYSOGENY SV40 SIMIAN VIRUS 40 T C R
TETRACYCLINE RESISTANT
T C S TETRACYCLINE SENSITIVE
|
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| dewey-search | 574.87/3282 |
| dewey-sort | 3574.87 43282 |
| dewey-tens | 570 - Biology |
| discipline | Biologie |
| format | Book |
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| id | DE-604.BV005958676 |
| illustrated | Not Illustrated |
| indexdate | 2024-07-09T16:37:35Z |
| institution | BVB |
| isbn | 0412161702 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-003737271 |
| oclc_num | 25449004 |
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| owner | DE-703 DE-29T |
| owner_facet | DE-703 DE-29T |
| physical | 78 S.: Ill. |
| publishDate | 1980 |
| publishDateSearch | 1980 |
| publishDateSort | 1980 |
| publisher | Chapman & Hall |
| record_format | marc |
| series2 | Outline studies in biology. |
| spelling | Glover, David M. Verfasser aut Genetic engineering cloning DNA London Chapman & Hall 1980 78 S.: Ill. txt rdacontent n rdamedia nc rdacarrier Outline studies in biology. Organisms - Recombinant DNA - Molecules - Cloning Clonage moléculaire DNA recombinant Cloning, Molecular DNA, Recombinant Molecular cloning Recombinant DNA SWB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=003737271&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Glover, David M. Genetic engineering cloning DNA Organisms - Recombinant DNA - Molecules - Cloning Clonage moléculaire DNA recombinant Cloning, Molecular DNA, Recombinant Molecular cloning Recombinant DNA |
| title | Genetic engineering cloning DNA |
| title_auth | Genetic engineering cloning DNA |
| title_exact_search | Genetic engineering cloning DNA |
| title_full | Genetic engineering cloning DNA |
| title_fullStr | Genetic engineering cloning DNA |
| title_full_unstemmed | Genetic engineering cloning DNA |
| title_short | Genetic engineering cloning DNA |
| title_sort | genetic engineering cloning dna |
| topic | Organisms - Recombinant DNA - Molecules - Cloning Clonage moléculaire DNA recombinant Cloning, Molecular DNA, Recombinant Molecular cloning Recombinant DNA |
| topic_facet | Organisms - Recombinant DNA - Molecules - Cloning Clonage moléculaire DNA recombinant Cloning, Molecular DNA, Recombinant Molecular cloning Recombinant DNA |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=003737271&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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