Gas chromatography in the analysis of steroid hormones:
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| Main Authors: | , |
|---|---|
| Format: | Book |
| Language: | English |
| Published: |
New York
Plenum Press
1966
|
| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Physical Description: | XVI, 288 S. Ill., zahlr. graph. Darst. |
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MARC
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| 100 | 1 | |a Wotiz, Herbert H. |e Verfasser |4 aut | |
| 245 | 1 | 0 | |a Gas chromatography in the analysis of steroid hormones |c Herbert H. Wotiz and Stanley J. Clark |
| 264 | 1 | |a New York |b Plenum Press |c 1966 | |
| 300 | |a XVI, 288 S. |b Ill., zahlr. graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
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| 650 | 4 | |a Chromatographie en phase gazeuse | |
| 650 | 4 | |a Hormones stéroïdes | |
| 650 | 4 | |a Chromatography, Gas | |
| 650 | 4 | |a Gas chromatography | |
| 650 | 4 | |a Steroid hormones |x Analysis | |
| 650 | 4 | |a Steroids |x analysis | |
| 700 | 1 | |a Clark, Stanley J. |e Verfasser |4 aut | |
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Record in the Search Index
| _version_ | 1804116686465400832 |
|---|---|
| adam_text | gas chromatography
in the analysis
of steroid hormones
Herbert H Wotiz
Professor of Biochemistry
and
Stanley J Clark
Research Associate in Biochemistry
Boston University, School of Medicine
« Boston, Massachusetts
PLENUM PRESS • NEW YORK -1966
Contents
Abbreviations xvi
Chapter 1: Introduction 1
Chapter 2: Definition of Chromatography 3
Gas Chromatography 3
The Column 3
Chapter 3: Gas Chromatographic Columns 5
Column Theory 5
Retention Volume 5
Partition Coefficients 7
Effect of Temperature on Retention Volume 7
Plate Theory 8
Peak Distortion i 0
Measurement of Retention Time 12
Selectivity of Stationary Phase 13
Column Performance 13
Spreading Due to Differences in Path Length 14
Diffusional Spreading 15
Resistance to Mass Transfer 16
Radial Diffusion in the Gas Phase 17
1
Chapter 4; Instrumentation 19
• Gas Controls 20
Injection Systems 21
Syringe Injection System 22
Syringes 24
Teriny-Harris Injector System 25
Solid Injection System 26
xi
xii Contents
Column Ovens and Temperature Controls 29
Detectors 30
Flame Ionization Detector 31
Electron Capture Detector 36
Electrometer Amplifiers 45
Recorders; 46
Connection Between Components of the Chromatograph 46
Chapter 5: Ancillary Methods 49
Trapping Methods 49
Qualitative Analysis 50
Ultraviolet Absorption Spectrometry 51
Optical Rotatory Dispersion 52
Infrared Spectrometry 52
Nuclear Magnetic Resonance Spectrometry 53
Mass Spectrometry 53
Quantitative Analysis 55
Accuracy 55
Precision 55
Peak Measurement 55
Measurement of Partially Separated Peaks 57
Calibration • 58
Chapter 6: The History of Gas-Liquid Chromatography of
Steroids 61
Chapter 7; Column Practice 69
Tubing Material 69
Column Configuration ; 69
Column Diameter 70
Solid Support 70
Pore Size 70
Adsorptivity of Support 70
Particle Size 71
Mechanical Strength 72
Stationary Phase 72
Volatility ^ 72
Stability 73
Selectivity 73
Column Preparation 73
Coating of Support 73
Column Packing V 7 4
Column Conditioning 75
Contents xi i i
Chapter 8: Decomposition of Steroids During GLC 77
Effect of Flash Heater 79
Effect of Column Packing 80
Effect of Column Container 82
Decomposition of Allylic Alcohols 84
/
Chapter 9: Irreversible Sorption 87
Chapter 10: Purity of Standards and Solvents 91
Standards 91
Solvents 91
Chapter 11: Choice of Derivative 95
Chapter 12: Preliminary Purification 97
Chapter 13: Relationship of Chromatographic Behavior and
Steroid Structure (by I S Hartman) 99
Measurement of Retention Time and Calculation of r Values 99
Steroid Numbers and T Values 122
Chapter 14: Determination of Progesterone and Its Metabolic
Products 129
Biochemist^ 129
Determination of Urinary Pregnanediol 131
Evaluation of Methods 131
Hydrolysis 146
Experimental 147
Method P-I (Kirschner and Lipsett) 147
Method P-II (Wotiz) 148
Determination of Progesterone 150
Evaluation of Methods 150
Experimental 157
Method PG-I (Yannone, McComas, and Goldfien) 157
Method PG-II (VanderMolen and Groen) 159
Chapter 15: Determination of Androgens and Related Steroids 165
Determination of 17-Ketosteroids 165
Biochemistry 165
Hydrolysis of Urinary 17-Ketosteroids 173
xiv Contents
•
Methods of Hydrolysis 174
Method 1; Mild Acid Hydrolysis (Vestergaard and
Claussen) 174
Method 2: Enzymatic Hydrolysis (Bush) 175
Method 3: Combined Enzyme Hydrolysis and Solvolysis
(Segal et al ) 175
Method 4: Solvolysis in the Presence of HC104 (DePaoli,
Nishizawa, and Eik-Nes) 176
Experimental 176
Method A-I (Hartman and Wotiz) 176
Method A-II (Kirschner and Lipsett) 177
Determination of Testosterone in Urine 178
Evaluation of Methods 178
Experimental 186
Method TU-I (Ibayashi et al ) 186
Method TU-II (Sparagana) 187
Determination of Testosterone in Plasma 191
Evaluation of Methods 191
Experimental 193
Method TP-I (Guerra-Garcia et al ) 193
Method TP-II (Brownie et al ) 196
Chapter 16: Determination of Urinary Pregnanetriol 201
i
Biochemistry and Evaluation of Methods 201
Experimental 203
Method PT-I (Rosenfeld) 203
Method PT-II (Kirschner and Lipsett) 206
Chapter 17: Analysis of Urinary Adrenocortical Steroids ; 207
Biochemistry and Evaluation of Methods 207
Experimental 219
Method GC-I (Rosenfeld) 219
Method GC-II (Bailey) 221
Chapter 18: Determination of Urinary Estrogens 225
Biochemistry and Evaluation of Methods 225
Choice of Derivative 227
Determination of Estrogens in Pregnancy Urine 232
Experimental 252
Materials and Procedures 252
Contents J xv
Method E-I: The Rapid Determination of Estrone and
Estriol in Pregnancy Urine (Wotiz and Martin) 258
Method E—II: Determination of the Three Classical
Estrogens Throughout Pregnancy Using Alumina and
Gas-Liquid Chromatography (Chattoraj and Wotiz) 261
Method E—HI; Determination of Seven Estrogens in
Pregnancy Using Thin-Layer and Gas-Liquid Chro
matography (Wotiz and Chattoraj) 261
Method E-IV: Analysis of Estrone, Estradiol, and
Estriol in Lower-Titer Urines (Chattoraj and Wotiz) 263
Chapter 19: Hopes and Needs for the Future 265
Directory of Suppliers 267
Bibliography 271
Author Index 275
Subject Index 279
|
| any_adam_object | 1 |
| author | Wotiz, Herbert H. Clark, Stanley J. |
| author_facet | Wotiz, Herbert H. Clark, Stanley J. |
| author_role | aut aut |
| author_sort | Wotiz, Herbert H. |
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| ctrlnum | (OCoLC)1925953 (DE-599)BVBBV002231583 |
| dewey-full | 544.926 |
| dewey-hundreds | 500 - Natural sciences and mathematics |
| dewey-ones | 544 - [Unassigned] |
| dewey-raw | 544.926 |
| dewey-search | 544.926 |
| dewey-sort | 3544.926 |
| dewey-tens | 540 - Chemistry and allied sciences |
| discipline | Chemie / Pharmazie |
| format | Book |
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| illustrated | Illustrated |
| indexdate | 2024-07-09T15:42:27Z |
| institution | BVB |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-001466498 |
| oclc_num | 1925953 |
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| physical | XVI, 288 S. Ill., zahlr. graph. Darst. |
| publishDate | 1966 |
| publishDateSearch | 1966 |
| publishDateSort | 1966 |
| publisher | Plenum Press |
| record_format | marc |
| spelling | Wotiz, Herbert H. Verfasser aut Gas chromatography in the analysis of steroid hormones Herbert H. Wotiz and Stanley J. Clark New York Plenum Press 1966 XVI, 288 S. Ill., zahlr. graph. Darst. txt rdacontent n rdamedia nc rdacarrier Chromatographie en phase gazeuse Hormones stéroïdes Chromatography, Gas Gas chromatography Steroid hormones Analysis Steroids analysis Clark, Stanley J. Verfasser aut HEBIS Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=001466498&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Wotiz, Herbert H. Clark, Stanley J. Gas chromatography in the analysis of steroid hormones Chromatographie en phase gazeuse Hormones stéroïdes Chromatography, Gas Gas chromatography Steroid hormones Analysis Steroids analysis |
| title | Gas chromatography in the analysis of steroid hormones |
| title_auth | Gas chromatography in the analysis of steroid hormones |
| title_exact_search | Gas chromatography in the analysis of steroid hormones |
| title_full | Gas chromatography in the analysis of steroid hormones Herbert H. Wotiz and Stanley J. Clark |
| title_fullStr | Gas chromatography in the analysis of steroid hormones Herbert H. Wotiz and Stanley J. Clark |
| title_full_unstemmed | Gas chromatography in the analysis of steroid hormones Herbert H. Wotiz and Stanley J. Clark |
| title_short | Gas chromatography in the analysis of steroid hormones |
| title_sort | gas chromatography in the analysis of steroid hormones |
| topic | Chromatographie en phase gazeuse Hormones stéroïdes Chromatography, Gas Gas chromatography Steroid hormones Analysis Steroids analysis |
| topic_facet | Chromatographie en phase gazeuse Hormones stéroïdes Chromatography, Gas Gas chromatography Steroid hormones Analysis Steroids analysis |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=001466498&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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