Agar gel electrophoresis:
Saved in:
| Main Author: | |
|---|---|
| Format: | Book |
| Language: | English |
| Published: |
Amsterdam [u.a.]
Elsevier
1965
|
| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Physical Description: | XIII, 425 S. Ill., graph. Darst. |
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| 245 | 1 | 0 | |a Agar gel electrophoresis |c by R. J. Wieme |
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| 650 | 7 | |a Electrophoresis |2 cabt | |
| 650 | 7 | |a Agar-agar |2 ram | |
| 650 | 7 | |a Electrophorèse sur gel |2 ram | |
| 650 | 7 | |a Électrophorèse sur gel |2 ram | |
| 650 | 4 | |a Agar | |
| 650 | 4 | |a Electrophoresis | |
| 650 | 4 | |a Gel electrophoresis | |
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Record in the Search Index
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| adam_text | AGAR GEL
ELECTROPHORESIS
by
R J WIEME
Head of the Laboratory for Clinical Chemistry,
Department of Internal Medicine,
University of Ghent, Belgium
ELSEVIERPUBLISHINGCOMPANY
AMSTERDAM/LONDON/NEWYORK
CONTENTS
Preface V
List of Abbreviations and Symbols XII
Chapter 1 Some Physical Aspects of Electrophoresis 1
1 Basic factors governing electrophoretic phenomena - 1
1 Electric charge 1
2 Electric field 4
3 Frictional forces 4
4 Electrophoretic mobility 5
2 Fundamental equations of electrophoretic movement 7
3 Electro-osmosis 9
4 Effects of heat production during electrophoresis 10
5 Applications of electrophoresis 13
6 Classification of electrophoretic techniques 13
7 Physicochemical aspects proper to zone electrophoresis 16
1 Aspects of z ?ne electrophoresis proper 17
2 Complications arising from the presence of a supporting medium 19
8 The place of agar gel electrophoresis among other lechniques using
a gelified electrophoretic medium 22
1 Silica gel 22
2 Pectin gel 22
3 Starch gel 22
4 Acrylamide gel (Cyanogum gel) 23
5 Carboxymethyl cellulose gel 24
6 Agar gel , 25
9 Conclusion 25
Chapter 2 Survey of Electrophoretic Techniques using Agar Gel 26
1 Pioneer work 26
2 First electrophoretic fractionation of proteins in an agar gel 27
V I I
CONTENTS
3 Agar gel electrophoresis in glass tubes 29
4 The immunoelectrophoretic technique of Grabar and Williams 31
5 Techniques related to that of Gordon-Grahar-Uriel 34
6 Column agar gel electrophoresis 45
7 Fluid film electrophoresis of Ressler 49
8 High voltage plate method of agar gel electrophoresis with cooling
and sealing 55
9 Conclusion 57
Chapter 3 Detailed Description of Three Typical Techniques Using
Agar Gel as an Electrophoretic Medium 58
1 Choice of three techniques 58
2 Electrophoresis according to Gordon-Grabar-Uriel 59
1 Preparation of the agar plates 59
2 Electrophoresis proper 61
3 Subsequent treatment of the plates 63
3 Fluid film electrophoresis according to Ressler and Zak 64
1 Preparation of the fluid film 65
2 Electrophoresis proper 66
3 Subsequent treatment of the film 67
4 High voltage plate method of agar gel electrophoresis with cooling,
sealing and thermostatic control 68
1 Preparation of the agar plates 68
2 Electrophoresis proper 69
3 Subsequent treatment of the plates 76
5 Conclusion 77
Addendum: list of commercially available equipment 77
Chapter 4 Special Problems 79
1 Quantitative aspects 79
1 Calculation of percentages 79
2 Measurement of electrophoretic mobilities 94
2 Chemistry and purification of agar 102
1 The origin of agar 102
2 Chemistry of agar 103
3 Purification of agar 104
4 Commercially available types of agar 109
3 Electro-osmosis 110
VIII
CONTENTS
4 Interactions occurring between agar gel and substances subjected to
electrophoresis in that medium 114
1 Adsorption 114
2 Viscosity effects 114
3 Molecular sieve effects 115
4 Precipitation 118
5 Ion exchange effects 122
6 Other types of interaction 123
5 Preparative applications of agar gel electrophoresis 126
6 Two-dimensional agar/starch gel electrophoresis 129
7 Conclusions 130
Chapter 5 Characterizing Reactions Applied to Agar Gel Pherograms 132
1 The importance of adding characterizing dimensions 10 electro
phoretic analysis 132
2 Survey of characterizing methods 132
3 Characterizing by physical properties 134
1 Optical properties J 34
2 Radioactivity 136
3 Precipitability 137
4 Addition of complexing substances 138
5 Molecular dimensions 138
4 Characterizing by chemical properties 138
1 Protein staiA 3 39
2 Lipid stains 141
3 Carbohydrate stains 142
4 Miscellaneous stains 146
5 Characterizing by enzymic properties 148
1 General considerations • 148
2 Oxidizing enzymes 151
3 Dehydrogenases 155
4 Transaminases 160
5 Hydrolytic enzymes 160
6 Characterizing by miscellaneous reactions 166
1 Pyroninophilic substances 166
2 Acridine orange stain 167
3 Gold-binding components 168
7 Immunoelectrophoretic characterization 168
I X
CONTENTS
1 The Immunoelectrophoresis concept 168
2 The reagent: antiserum 170
3 Procedure 174
8 Conclusions 180
Chapter 6 Applications 182
Introduction 182
1 Applications to the study of non-protein substances 182
1 Inorganic ions 182
2 Alkaloids 182
3 Phenols 184
4 Oligonucleotides 184
5 Nucleic acids 185
6 Porphyrins 186
7 Fatty acids 186
2 Applications to the study of proteins 187
1 Haemoglobin 187
2 Proteins of micro-organisms 203
3: Proteins of animal blood 209
4 Human serum proteins 221
1 Normal human serum 222
2 Human serum proteins in pathological conditions 251
5 VariouSjbiological fluids 274
1 Human amniotic fluid 274
2 Aqueous humour 275
3 Human ascitic fluid 276
4 Human bile 276
5 Cerebrospinal fluid 277
6 Humour of the human corpus vitreum 285
7 Gastric, duodenal and pancreatic juice 285
8 Milk 287
9 Perilymph (of the inner ear) 288
10 Human saliva 288
11 Human seminal plasma 289
12 Human synovial fluid 290
13 Tears 291
14 Human urine 292
6 Tissue proteins 299
x
CONTENTS
1 Introductory remarks 299
2 Eye lens proteins 303
3 Leucocytes 311
4 Liver tissue 312
5 Gastric mucosa 315
6 Skeletal muscle 315
7 Central nervous system 318
8 Human prostate 321
9 Other tissues 322
10 Related applications 323
7 Enzymes 324
1 Dehydrogenases 324
2 Tyrosinase 341
3 Transaminase 342
4 Urease 342
5 Carboxylic esterases 342
6 Phosphomonoesterases (phosphatases) 346
7 DN-ases 348
8 Proteases 348
References 353
A Directly related to agar gel electrophoresis 355
B Indirectly related to agar gel electrophoresis 388
Author Index 395
Subject Index 404
X I
|
| any_adam_object | 1 |
| author | Wieme, Roger J. |
| author_facet | Wieme, Roger J. |
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| author_sort | Wieme, Roger J. |
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| dewey-ones | 574 - [Unassigned] |
| dewey-raw | 574.1920724 |
| dewey-search | 574.1920724 |
| dewey-sort | 3574.1920724 |
| dewey-tens | 570 - Biology |
| discipline | Biologie Chemie |
| format | Book |
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| id | DE-604.BV002052055 |
| illustrated | Illustrated |
| indexdate | 2024-07-09T15:39:31Z |
| institution | BVB |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-001341885 |
| oclc_num | 1116004 |
| open_access_boolean | |
| owner | DE-19 DE-BY-UBM DE-83 DE-188 |
| owner_facet | DE-19 DE-BY-UBM DE-83 DE-188 |
| physical | XIII, 425 S. Ill., graph. Darst. |
| psigel | TUB-nveb |
| publishDate | 1965 |
| publishDateSearch | 1965 |
| publishDateSort | 1965 |
| publisher | Elsevier |
| record_format | marc |
| spelling | Wieme, Roger J. Verfasser aut Agar gel electrophoresis by R. J. Wieme Amsterdam [u.a.] Elsevier 1965 XIII, 425 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Electrophoresis cabt Agar-agar ram Electrophorèse sur gel ram Électrophorèse sur gel ram Agar Electrophoresis Gel electrophoresis Gels Gelelektrophorese (DE-588)4123267-7 gnd rswk-swf Gelelektrophorese (DE-588)4123267-7 s DE-604 HEBIS Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=001341885&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Wieme, Roger J. Agar gel electrophoresis Electrophoresis cabt Agar-agar ram Electrophorèse sur gel ram Électrophorèse sur gel ram Agar Electrophoresis Gel electrophoresis Gels Gelelektrophorese (DE-588)4123267-7 gnd |
| subject_GND | (DE-588)4123267-7 |
| title | Agar gel electrophoresis |
| title_auth | Agar gel electrophoresis |
| title_exact_search | Agar gel electrophoresis |
| title_full | Agar gel electrophoresis by R. J. Wieme |
| title_fullStr | Agar gel electrophoresis by R. J. Wieme |
| title_full_unstemmed | Agar gel electrophoresis by R. J. Wieme |
| title_short | Agar gel electrophoresis |
| title_sort | agar gel electrophoresis |
| topic | Electrophoresis cabt Agar-agar ram Electrophorèse sur gel ram Électrophorèse sur gel ram Agar Electrophoresis Gel electrophoresis Gels Gelelektrophorese (DE-588)4123267-7 gnd |
| topic_facet | Electrophoresis Agar-agar Electrophorèse sur gel Électrophorèse sur gel Agar Gel electrophoresis Gels Gelelektrophorese |
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| work_keys_str_mv | AT wiemerogerj agargelelectrophoresis |