Correlative microscopy in biology: instrumentation and methods
Saved in:
| Format: | Book |
|---|---|
| Language: | English |
| Published: |
Orlando u.a.
Acad. Pr.
1987
|
| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Physical Description: | XIII, 437 S. Ill. |
| ISBN: | 0123339227 |
Staff View
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| 245 | 1 | 0 | |a Correlative microscopy in biology |b instrumentation and methods |c ed. by M. A. Hayat |
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Record in the Search Index
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| adam_text | Correlative
Microscopy
in Biology
Instrumentation and Methods
Edited by
M A HAYAT
Department of Biology
Kean College of New Jersey
Union, New Jersey
ACADEMIC PRESS, INC
Harcourt Brace Jovanovich, Publishers
Orlando San Diego New York Austin
Boston London Sydney Tokyo Toronto
Contents
Preface xi
1 Biological Specimen Preparation for
Correlative Light and Electron Microscopy
David T Moran and J Carter Rowley
Introduction 2
Methods 4
Applications of Correlative Light and Electron Microscopy 17
References 22
2 Light and Scanning Electron Microscopy in a
Combined Instrument
Cornelia H Wouters and J S Ploem
Introduction
Description of the Combined Instrument
Preparation and Staining Method for LM/SEM Specimens
Quantitative Measurements in the LM/SEM
r
Cathodoluminescence in the LM/SEM
Biomedical Applications
Developments with Another Type of Scanning Electron
Microscope t
Conclusions
References
vi
f Contents
3 Confocal Microscopy in Comparison with
Electron and Conventional Light Microscopy
HTM van der Voort, JAC Valkenburg,
E A van Spronsen, C L Woldringh, and G J Brakenhoff
Introduction 60
The CSLM Instrument 61
The Morphology of the Nucleoid of Escherichia coli 66
Characterization of Cell Shape 70
3-D Imaging Techniques with CSLM 75
Conclusion and Outlook 78
References 79
Combined Light and Electron Microscopy of
Central Nervous System Neurons and Their
Afferent and Efferent Synaptic Connections
F G Wouterlood
Introduction - 84
Morphological Techniques: Correlative Light and Electron
Microscopy of Golgi-Impregnated Neurons 86
Immunocytochemical Techniques: Correlative Light and Electron
Microscopy of Neurons Labeled Immunocytochemically for the
Presence of Neuroactive Substances of Their Synthesizing
Enzymes 94
Correlative Light and Electron Microscopy of Neurons Labeled
with PHA-L 101
References 109
Intracellular Staining of Neurons
in the Central Nervous System: Combined
Physiological, Morphological, and Cytological
Analysis of Identified Neurons
Georgia A Bishop and James S King
Introduction 122
Methods 126
Tissue Processing 128
Data Analysis
t 131
Limitations of the Method 136
Conclusions 138
References 139
Contents vii
6 Correlative Microscopy of Catecholamines
and Neuropeptides in Adrenal Medulla
Markku Pelto-Huikko
Introduction 144
Vibratome Sectioning and Fluorescence Microscopy 144
Immunocytochemistry 147
Possible Modifications 151
Double:Labeling Methods for Studying Coexistence 152
Advantages and Limitations of Combined Faglu-Fluorescence and
Immunocytochemistry Method 153
References • 154
7 Sequential Immunocytochemical and Silver
Staining of Neuroendocrine Cells in the
Same Section
Erik Wilander and Monalill Lundqvist
Introduction 158
Description of Sequential Staining Technique • 158
Comments on Methodology 160
Applications 161
References 163
8 Correlative Light and Electron Microscopic
Method for the Visualization of the Same
in Vitro Cell Using Radioautography and
Serial Sectioning
Jacqueline Vuillet
Introduction 166
Methodology 166
Applications 170
References 171
9 Correlative Light and Transmission
Electron Microscopy of Mitotic Cells
in the Nervous System
R R Sturrock
Introduction t 173
Mitotic Cells in the Embryonic CNS 174
Mitotic Cells in the CNS during Myelination 179
vlii Contents
Identification of Mitotic Cells in the Mature and Aged CNS 185
Applications 185
References 185
10 Light and Scanning Electron Microscopy
of the Same Metaphase Chromosomes
Christine J Harrison, Elspeth M Jack, and Terence D Allen
Introduction 190
Methodology 191
Applications of Correlative Light Microscopy and SEM 232
References 244
11 Correlative Light and Electron Microscopy
on the Same Epoxy Section
Conly L Rieder and Samuel S Bowser
Introduction 250
Methodology 251
Applications 262
References 274
12 Correlative Microscopy of Skeletal Muscle
and Endometrium
H Dieter Geissinger, Peter A Rhodes,
and Lorraine D Rhodes
Introduction 280
Materials and Methods for Skeletal Muscle 282
Materials and Methods for Endometrium 285
Discussion of Methodology and Selected Results 289
References 300
; 13 Correlative Microscopy of Isolated Glomeruli
S B Or and Lily Ma
Introduction 303
Method for Correlative Microscopy t 304
Illustrated Examples 315
Concluding Remarks 326
References 328
Contents IX
14 Correlative Microscopy of Cell Surface and
Underlying Structures
Jon B Reitan, Kari Feren, Franz Thiebaut,
and Albrecht Reith
Introduction 332
Techniques Using Light Microscopy for Cytological Features in
Combination with Scanning Electron Microscopy for
Surface Structures 333
Techniques Using fiackscattered and Secondary Electron Imaging
on Cells and Tissues Processed for Scanning Electron Microscopy 338
Applications and Future Prospects 343
References 344
15 Correlative Light, Scanning, and
Transmission Electron Microscopy of
Deembedded Tissues
Hisatake Kondo
Introduction 347
Methodology 348
Applications 350
References 354
16 Immunoelectron Microscopic Mirror Method:
A Correlative Study
Hiroshi Takagi
Introduction 355
Methodology 359
Correlative Light and Electron Microscopy 360
Discussion 363
References 364
17 The Detection of Amyloid Using Correlative
Polarizing Light Microscopy and Electron
Microscopy
David C Cottell and John G Bannigan
Introduction 368
Amyloidosis
x 370
Methods 375
Concluding Remarks 384
References 385
Contents
18 Correlative Microscopy of Human
Vitreous Body
Juergen Faulborn
Introduction 389
Method 390
Conclusion 396
References 397
19 A New Method for Bacterial Identification
by Studying the Same Section with Light
Microscopy and Scanning and Transmission
Electron Microscopy
F R Saglie, J C Sa Ferreira, and P L Valentin
Introduction 400
Light Microscopy, Scanning and Transmission Electron
Microscopy as Separated Single Tool for Detecting Bacteria
within Gingival Tissues 400
Method 401
Application 404
References • 406
20 Correlative Light and Scanning
Electron Microscopy of Thick Sections
and Isolated Cells
Subhas C Mohapatra and Harold E Pattee
Introduction
Operating Principles
Application of Correlative Scanning Electron Microscopy
Stepwise Procedure
Conclusion
References
Index 433
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| dewey-tens | 570 - Biology |
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| spelling | Correlative microscopy in biology instrumentation and methods ed. by M. A. Hayat Orlando u.a. Acad. Pr. 1987 XIII, 437 S. Ill. txt rdacontent n rdamedia nc rdacarrier Microscopie - Technique Microscopy Technique Microscopy instrumentation Microscopy methods Mikroskopie (DE-588)4039238-7 gnd rswk-swf Biologie (DE-588)4006851-1 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Mikroskopie (DE-588)4039238-7 s Biologie (DE-588)4006851-1 s DE-604 Hayat, M. A. 1936- Sonstige (DE-588)129731447 oth HEBIS Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=000804749&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Correlative microscopy in biology instrumentation and methods Microscopie - Technique Microscopy Technique Microscopy instrumentation Microscopy methods Mikroskopie (DE-588)4039238-7 gnd Biologie (DE-588)4006851-1 gnd |
| subject_GND | (DE-588)4039238-7 (DE-588)4006851-1 (DE-588)4143413-4 |
| title | Correlative microscopy in biology instrumentation and methods |
| title_auth | Correlative microscopy in biology instrumentation and methods |
| title_exact_search | Correlative microscopy in biology instrumentation and methods |
| title_full | Correlative microscopy in biology instrumentation and methods ed. by M. A. Hayat |
| title_fullStr | Correlative microscopy in biology instrumentation and methods ed. by M. A. Hayat |
| title_full_unstemmed | Correlative microscopy in biology instrumentation and methods ed. by M. A. Hayat |
| title_short | Correlative microscopy in biology |
| title_sort | correlative microscopy in biology instrumentation and methods |
| title_sub | instrumentation and methods |
| topic | Microscopie - Technique Microscopy Technique Microscopy instrumentation Microscopy methods Mikroskopie (DE-588)4039238-7 gnd Biologie (DE-588)4006851-1 gnd |
| topic_facet | Microscopie - Technique Microscopy Technique Microscopy instrumentation Microscopy methods Mikroskopie Biologie Aufsatzsammlung |
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