Oligodendrocytes and myelin lipid synthesis:
Saved in:
| Main Author: | |
|---|---|
| Format: | Thesis Book |
| Language: | English |
| Published: |
1985
|
| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Item Description: | Utrecht, Univ., Diss. - PST: Oligodendroycten en de synthese van myeline lipiden |
| Physical Description: | 140 S. Ill., graph. Darst. |
Staff View
MARC
| LEADER | 00000nam a2200000 c 4500 | ||
|---|---|---|---|
| 001 | BV001116553 | ||
| 003 | DE-604 | ||
| 005 | 00000000000000.0 | ||
| 007 | t | ||
| 008 | 890315s1985 ad|| m||| 00||| eng d | ||
| 035 | |a (OCoLC)615591602 | ||
| 035 | |a (DE-599)BVBBV001116553 | ||
| 040 | |a DE-604 |b ger |e rakddb | ||
| 041 | 0 | |a eng | |
| 049 | |a DE-19 |a DE-188 | ||
| 100 | 1 | |a Koper, Frans J. |e Verfasser |4 aut | |
| 245 | 1 | 0 | |a Oligodendrocytes and myelin lipid synthesis |c door Frans Jan Willem Koper |
| 246 | 1 | 3 | |a Oligodendrocyten en de synthese van myeline lipiden |
| 264 | 1 | |c 1985 | |
| 300 | |a 140 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 500 | |a Utrecht, Univ., Diss. - PST: Oligodendroycten en de synthese van myeline lipiden | ||
| 502 | |a Utrecht, Univ., Diss., 1985 | ||
| 655 | 7 | |0 (DE-588)4113937-9 |a Hochschulschrift |2 gnd-content | |
| 856 | 4 | 2 | |m HBZ Datenaustausch |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=000671353&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
| 999 | |a oai:aleph.bib-bvb.de:BVB01-000671353 | ||
Record in the Search Index
| _version_ | 1804115522820767744 |
|---|---|
| adam_text | CONTENTS
ABBREVIATIONS 11
CHAPTER I INTRODUCTION 13
1.1 RESEARCH ON GLIAL CELLS 15
1.2 CELLS IN THE CENTRAL NERVOUS SYSTEM 15
1.3 OLIGODENDROCYTES 16
1.4 MYELIN 18
1.5 MYELIN COMPOSITION 20
1.6 KETONE BODIES AND THE SYNTHESIS OF BRAIN LIPIDS 22
1.7 A SURVEY OF THIS THESIS 22
CHAPTER II CULTURE AND CHARACTERIZATION OF RAT CEREBRAL OLIGODENDRO¬
CYTES IN A SERUM FREE CHEMICALLY DEFINED MEDIUM 25
II. 1 INTRODUCTION 27
11.1.1 Isolation and culture of oligodendrocytes 27
11.1.2 Serum free culture media 29
1I.2 MATERIALS AND METHODS 30
11.2.1 Materials 30
11.2.2 Animals 30
11.2.3 Isolation of oligodendrocytes 30
11.2.4 Culture conditions 32
11.2.5 Immunofluorescence 32
11.2.6 Enzyme assays 33
11.2.7 Complement mediated cytolysis of oligodendrocytes 34
11.2.8 Incubations with radioactive substrates 34
11.2.9 Other methods 35
11.3 RESULTS 35
11.3.1 Development and morphology of the cells in culture 35
11.3.2 Synthesis of oligodendrocyte specific lipids 42
11.3.3 Oligodendrocyte and myelin specific enzymes 44
11.4 DISCUSSION 45
11.4.1 Development of oligodendrocytes in culture 45
11.4.2 Complement mediated cytolysis 50
11.4.3 Oligodendrocyte associated enzymes 50
11.5 CONCLUSIONS 53
I
CHAPTER III KETONE BODIES AND GLUCOSE AS PRECURSORS FOR MYELIN LIPID
SYNTHESIS IN VIVO AND IN VITRO 55
111.1 INTRODUCTION 57
111.2 MATERIALS AND METHODS 59
111.2.1 Studies in vivo 59 j
111.2.2 Studies with oligodendrocyte enriched cultures 61 j
111.2.3 Measurement of acetoacetyl CoA synthetase 62
111.3 RESULTS 63
111.3.1 Cerebral subcellular fractions labeled in vivo 63
111.3.2 Incorporation in vivo of label from [^Cjglucose,
p ^C] acetoacetate and 3H2O into fatty acids and
sterols of homogenates and myelin prepared from
whole cerebra and spinal cord 67
1.1.3,3 Acetoacetate and glucose as precursors for the
synthesis of fatty acids and cholesterol in
oligodendrocytes in culture 69
111.3.4 Absolute rates of lipogenesis and cholesterogenesis 73
111.3.5 The activity of acetoacetyl CoA synthetase in
oligodendrocytes and astrocytes 73
III.4 DISCUSSION 74
111.4.1 Glucose and ketone bodies as precursors for brain
lipogenesis and cholesterogenesis in vivo 74
111.4.2 Glucose and ketone bodies as precursors for
lipogenesis and cholesterogenesis in cultured
oligodendrocytes 76
111.4.3 Acetoacetate as a highly cholesterogenic substrate 77
111.4.4 Heterogeneity in the brain with respect to the
utilization of ketone bodies for lipid synthesis in
vivo 77
111.4.5 Absolute rates of lipogenesis and cholesterogenesis
from glucose and acetoacetate 79
III.5 CONCLUSIONS 80
111.5.1 From studies in vivo 80
111.5.2 From studies in vitro 80
CHAPTER IV THE METABOLISM OF COMPLEX LIPIDS IN CULTURED OLIGODENDRO
CYTES; ASPECTS OF HORMONAL REGULATION 81
IV.1 INTRODUCTION 83
IV.1.1 Sulfolipids in the brain 83
IV.1.2 Effects of hormones on sulfolipid metabolism and
myelination 85
IV.1.3 Polyphosphoinositides 87
IV.1.4 Scope of the experiments described in this chapter 89
IV.2 MATERIALS AND METHODS 90
IV.2.1 Cell cultures 90
IV.2.2 Analysis of the incorporation of [35S]sulfate 90
IV.2.3 Cell counts 91
IV.2.4 Analysis of the incorporation of [ P]phosphate 91
IV.3 RESULTS 92
IV.3.1 Synthesis of sulfolipids by oligodendrocytes 92
IV.3.2 Hormonal influences on oligodendroglial sulfolipid
synthesis 93
IV.3.3 Triiodothyronine, hydrocortisone and the
development of oligodendrocytes in culture 98
IV.3.4 Sulfolipid degradation 99
IV.3.5 Inositide metabolism in oligodendrocytes and
astrocytes in culture 100
IV.4 DISCUSSION 104
IV.4.1 Sulfolipid metabolism in vivo and in vitro 104
IV.4.2 Sulfolipid degradation 105
IV.4.3 The influence of hormones on sulfolipid metabolism 106
IV.4.4 Polyphosphoinositide metabolism in cultured
oligodendrocytes and astrocytes 109
IV.5 CONCLUSIONS 110
CHAPTER V SUMMARIZING DISCUSSION AND FUTURE POSSIBILITIES 111
V.I UTILIZATION OF KETONE BODIES AND GLUCOSE FOR MYELIN
LIPID SYNTHESIS IN VIVO AND IN VITRO 113
V.2 DIFFERENCES BETWEEN OBSERVATIONS IN VIVO AND IN
VITRO 115
V.3 OLIGODENDROCYTES IN CULTURE 116
V.4 HORMONAL INFLUENCES ON LIPID SYNTHESIS BY CULTURED
OLIGODENDROCYTES 117
j
I
I
V.5 FUTURE POSSIBILITIES 118 !
i
}
REFERENCES 121 I
SAMENVATTING 134
NAWOORD 138
]
CURRICULUM VITAE 140 !
1
|
| any_adam_object | 1 |
| author | Koper, Frans J. |
| author_facet | Koper, Frans J. |
| author_role | aut |
| author_sort | Koper, Frans J. |
| author_variant | f j k fj fjk |
| building | Verbundindex |
| bvnumber | BV001116553 |
| ctrlnum | (OCoLC)615591602 (DE-599)BVBBV001116553 |
| format | Thesis Book |
| fullrecord | <?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01188nam a2200301 c 4500</leader><controlfield tag="001">BV001116553</controlfield><controlfield tag="003">DE-604</controlfield><controlfield tag="005">00000000000000.0</controlfield><controlfield tag="007">t</controlfield><controlfield tag="008">890315s1985 ad|| m||| 00||| eng d</controlfield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(OCoLC)615591602</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)BVBBV001116553</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-604</subfield><subfield code="b">ger</subfield><subfield code="e">rakddb</subfield></datafield><datafield tag="041" ind1="0" ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="049" ind1=" " ind2=" "><subfield code="a">DE-19</subfield><subfield code="a">DE-188</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Koper, Frans J.</subfield><subfield code="e">Verfasser</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Oligodendrocytes and myelin lipid synthesis</subfield><subfield code="c">door Frans Jan Willem Koper</subfield></datafield><datafield tag="246" ind1="1" ind2="3"><subfield code="a">Oligodendrocyten en de synthese van myeline lipiden</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">1985</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">140 S.</subfield><subfield code="b">Ill., graph. Darst.</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="b">n</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="b">nc</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="500" ind1=" " ind2=" "><subfield code="a">Utrecht, Univ., Diss. - PST: Oligodendroycten en de synthese van myeline lipiden</subfield></datafield><datafield tag="502" ind1=" " ind2=" "><subfield code="a">Utrecht, Univ., Diss., 1985</subfield></datafield><datafield tag="655" ind1=" " ind2="7"><subfield code="0">(DE-588)4113937-9</subfield><subfield code="a">Hochschulschrift</subfield><subfield code="2">gnd-content</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="m">HBZ Datenaustausch</subfield><subfield code="q">application/pdf</subfield><subfield code="u">http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=000671353&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA</subfield><subfield code="3">Inhaltsverzeichnis</subfield></datafield><datafield tag="999" ind1=" " ind2=" "><subfield code="a">oai:aleph.bib-bvb.de:BVB01-000671353</subfield></datafield></record></collection> |
| genre | (DE-588)4113937-9 Hochschulschrift gnd-content |
| genre_facet | Hochschulschrift |
| id | DE-604.BV001116553 |
| illustrated | Illustrated |
| indexdate | 2024-07-09T15:23:57Z |
| institution | BVB |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-000671353 |
| oclc_num | 615591602 |
| open_access_boolean | |
| owner | DE-19 DE-BY-UBM DE-188 |
| owner_facet | DE-19 DE-BY-UBM DE-188 |
| physical | 140 S. Ill., graph. Darst. |
| publishDate | 1985 |
| publishDateSearch | 1985 |
| publishDateSort | 1985 |
| record_format | marc |
| spelling | Koper, Frans J. Verfasser aut Oligodendrocytes and myelin lipid synthesis door Frans Jan Willem Koper Oligodendrocyten en de synthese van myeline lipiden 1985 140 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Utrecht, Univ., Diss. - PST: Oligodendroycten en de synthese van myeline lipiden Utrecht, Univ., Diss., 1985 (DE-588)4113937-9 Hochschulschrift gnd-content HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=000671353&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Koper, Frans J. Oligodendrocytes and myelin lipid synthesis |
| subject_GND | (DE-588)4113937-9 |
| title | Oligodendrocytes and myelin lipid synthesis |
| title_alt | Oligodendrocyten en de synthese van myeline lipiden |
| title_auth | Oligodendrocytes and myelin lipid synthesis |
| title_exact_search | Oligodendrocytes and myelin lipid synthesis |
| title_full | Oligodendrocytes and myelin lipid synthesis door Frans Jan Willem Koper |
| title_fullStr | Oligodendrocytes and myelin lipid synthesis door Frans Jan Willem Koper |
| title_full_unstemmed | Oligodendrocytes and myelin lipid synthesis door Frans Jan Willem Koper |
| title_short | Oligodendrocytes and myelin lipid synthesis |
| title_sort | oligodendrocytes and myelin lipid synthesis |
| topic_facet | Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=000671353&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT koperfransj oligodendrocytesandmyelinlipidsynthesis AT koperfransj oligodendrocytenendesynthesevanmyelinelipiden |