Immunocytochemistry:
Saved in:
| Main Author: | |
|---|---|
| Format: | Book |
| Language: | English |
| Published: |
New York [u.a.]
Wiley
1986
|
| Edition: | 3. ed. |
| Series: | A Wiley medical publication
|
| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Item Description: | Literaturangaben |
| Physical Description: | XIII, 524 S. zahlr. Ill., graph. Darst. |
| ISBN: | 0471867217 |
Staff View
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| 100 | 1 | |a Sternberger, Ludwig A. |e Verfasser |4 aut | |
| 245 | 1 | 0 | |a Immunocytochemistry |
| 250 | |a 3. ed. | ||
| 264 | 1 | |a New York [u.a.] |b Wiley |c 1986 | |
| 300 | |a XIII, 524 S. |b zahlr. Ill., graph. Darst. | ||
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| 650 | 4 | |a Histocytochemistry | |
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Record in the Search Index
| _version_ | 1804115478976659456 |
|---|---|
| adam_text | Immunocytochemistry
Third Edition
Ludwig A Sternberger, M D
The University of Rochester School of Medicine and Dentistry
A Wiley Medical Publication
JOHN WILEY amp; SONS
New York Chichester Brisbane Toronto
Contents
1 IMMUNOGLOBULIN STRUCTURE: INTRODUCTION TO
IMMUNOCYTOCHEMISTRY 1
Common features of the four-chain immunoglobulin monomer 4
Immunoglobulin G 6
Immunoglobulin domains 10
Genes in fragments 11
Allotypy 14
Idiotypy 14
The primary function of antibodies: Affinity for antigen 16
The precipitin reaction 19
Electroblot immunocytochemistry 20
Radioimmunoassay 20
Immunoglobulin isotypes and the secondary, biologic functions
of antibodies 25
Affinity purification of antibodies 27
2 CONJUGATED ANTIBODY METHODS 32
Use of markers 33
Immunofluorescence 34
Conjugation of antibody with fluorescein isothiocyanate:
Principles 39
Preparation of fluorescein isothiocyanate-immunoglobulin
conjugate (FITC-Ig): Procedure 40
Conjugation of purified antibodies 42
Direct and indirect staining modes 42
ix
X CONTENTS
Staining of living cells 44
Fluorescent cell sorting 46
Specificity 47
Immunofluorescence method specificity 48
Quantitative immunofluorescence 53
Immunofluorescence microscopy and photography 54
Enzyme-conjugated antibodies 56
Conjugation of peroxidase to immunoglobulin with
glutaraldehyde 63
The periodate conjugation technique 64
Use of antibody fragments in conjugation 64
Conjugation of peroxidase to Fab hinge regions with maleimide 65
Nonperoxidase enzymes for conjugation with antibodies 66
Signal intensification for light microscopy in conjugated
antibody methods 67
Conjugation of biotinylated peroxidase and antibody with avidin 67
Electron microscopy with peroxidase-conjugated antibodies 70
The immunoferritin method 72
Immunogold techniques 79
Method specificity of conjugates of antibodies with enzymes or
other proteins 83
3 THE UNLABELED ANTIBODY PEROXIDASE-ANTIPEROXIDASE
(PAP) METHOD 90
Preparation of soluble PAP complex 96
Properties of PAP 100
Staining for light microscopy 103
Postembedcling-staining for electron microscopy 114
Pre-embedding staining for electron microscopy 126
Method specificity 142
Identification of background 145
Quantitative immunocytochemistry 146
Sensitivity 164
Resolution 175
Variations of the unlabeled antibody PAP method 180
Combination of PAP immunocytochemistry with tracer uptake
studies in the nervous system 186
Combination of PAP immunocytochemistry with
autoradiography 190
Staining of two antigens in the same section 193
Reversibility of the immunocytochemical reaction 200
4 TISSUE PREPARATION
CONTENTS xi
Fixatives 212
Fixation strategies 217
Sectioning 218
5 SPECIFICITY OF ANTISERA 225
Causes of impaired specificity 226
Tests for specificity 230
Strategies for testing specificity of antisera 243
6 SPECIFICITY OF MONOCLONAL ANTIBODIES 245
Preparation of monoclonal antibodies: Principle 247
Methods of clone selection 250
Preparation of monoclonal antibodies for immunocytochemistry:
Procedure 252
Interpretation of specificity 256
Staining 258
Affinity purification of antigens 262
Strategies for use of monoclonal antibodies 262
7 THE IMMUNE RESPONSE—LYMPHOMAS 270
Common B-lmd T-cell functions 274
Specific B-cell functions 277
B-cell memory 280
B-cell tolerance 280
Specific A-cell functions 282
Specific T helper cell functions 283
Specific T suppressor cell functions 283
Memory T cells 284
Specific function of natural killer cells 284
B-cell differentiation 285
Distribution of lymphocytes in lymphoid tissue 285
Lymphomas 288
Clinical pathology of peripheral leukocytes 298
tii CONTENTS
8 NEUROCYTOCHEMISTRY—NEUROPATHOLOGY 305
Antibodies to neurotransmitters and their biosynthetic enzymes 308
Serotonin 309
Taurine 313
Gamma-aminobutyric and glutamic acids 314
Catecholamines 316
Acetylcholine 317
Neurotransmitter colocalization 317
Identification of pathways 322
Neurotransmitter pathology 328
Familial dysautonomia 330
Olfactory protein 333
Neuron-specific enolase 333
Protein S-100 335
Glial fibrillary acidic protein 335
Brain tumors 339
Myelin 341
9 REGULATORY PEPTIDES—ENDOCRINE PATHOLOGY 357
The Scharrer concept of neurosecretion 358
Opioid and other stress-related peptides 359
Substance P 367
Somatostatin 367
Corticotropin-releasing factor 372
Oxytocin and vasopressin 379
Luteinizing hormone-releasing hormone 384
Thyrotropin-releasing hormone 388
Cholecystokinin 389
Vasoactive intestinal peptide 392
Polypeptide YY 393
FMRF-amide 394
Evolutionary aspects 394
Chemoarchitectonic patterns 395
Colocalization 397
Hormone receptors 401
Diabetes mellitus 414
Endocrine tumors 415
CONTENTS xiii
10 NEURONAL DIVERSITY 427
Identification of newly detected antigens 432
Heterogeneity of neurofilaments 438
Antigenicity of epitopes from heterogeneity regions of
neurofilaments 446
Monoclonal antibodies distinguish phosphorylated and
nonphosphorylated forms of neurofilaments in situ 448
Microheterogeneity of post-translational change 455
Epitopes of early and late appearance in development 458
Chemoarchitectonics and the scope of modern
immunocytochemistry 460
Alzheimer s disease 461
11 PROSPECTIVE PATHOLOGY 467
Antigenic expression of tumor differentiation 468
Carcinoembryonic antigen 470
Extragonadal germ cell cancer syndrome 471
Metastatic tumors 472
12 AUTOIMMUNE DISEASES 475
Deficiency of T suppressor cells 476
Idiotypic antibodies 477
Pathogenicity 478
Glomerulonephritis 478
Goodpasture s syndrome 479
Immune complex disease 480
Autoimmune thyroiditis 482
Lupus erythematosus 482
Myasthenia gravis 484
Multiple sclerosis 485
Experimental allergic encephalomyelitis 487
Demyelinating polyneuropathy 490
Author Index 495
Subject Index 511
|
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| bvnumber | BV001061969 |
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| dewey-full | 574.2/9 |
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| dewey-ones | 574 - [Unassigned] |
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| dewey-search | 574.2/9 |
| dewey-sort | 3574.2 19 |
| dewey-tens | 570 - Biology |
| discipline | Biologie Chemie Medizin |
| edition | 3. ed. |
| format | Book |
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| id | DE-604.BV001061969 |
| illustrated | Illustrated |
| indexdate | 2024-07-09T15:23:16Z |
| institution | BVB |
| isbn | 0471867217 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-000642625 |
| oclc_num | 12052490 |
| open_access_boolean | |
| owner | DE-19 DE-BY-UBM DE-12 DE-29 DE-188 |
| owner_facet | DE-19 DE-BY-UBM DE-12 DE-29 DE-188 |
| physical | XIII, 524 S. zahlr. Ill., graph. Darst. |
| publishDate | 1986 |
| publishDateSearch | 1986 |
| publishDateSort | 1986 |
| publisher | Wiley |
| record_format | marc |
| series2 | A Wiley medical publication |
| spelling | Sternberger, Ludwig A. Verfasser aut Immunocytochemistry 3. ed. New York [u.a.] Wiley 1986 XIII, 524 S. zahlr. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier A Wiley medical publication Literaturangaben Histocytochemistry Immunochemistry Immuncytochemie (DE-588)4161368-5 gnd rswk-swf Immuncytochemie (DE-588)4161368-5 s DE-604 HEBIS Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=000642625&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Sternberger, Ludwig A. Immunocytochemistry Immunocytochemistry Histocytochemistry Immunochemistry Immuncytochemie (DE-588)4161368-5 gnd |
| subject_GND | (DE-588)4161368-5 |
| title | Immunocytochemistry |
| title_auth | Immunocytochemistry |
| title_exact_search | Immunocytochemistry |
| title_full | Immunocytochemistry |
| title_fullStr | Immunocytochemistry |
| title_full_unstemmed | Immunocytochemistry |
| title_short | Immunocytochemistry |
| title_sort | immunocytochemistry |
| topic | Immunocytochemistry Histocytochemistry Immunochemistry Immuncytochemie (DE-588)4161368-5 gnd |
| topic_facet | Immunocytochemistry Histocytochemistry Immunochemistry Immuncytochemie |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=000642625&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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