A guidebook to lipoprotein technique:
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Book |
| Language: | English |
| Published: |
Amsterdam u.a.
Elsevier
1984
|
| Series: | Laboratory techniques in biochemistry and molecular biology
14 |
| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Item Description: | Literaturverz. S. 491 - 507 |
| Physical Description: | XIV, 512 S. Ill., graph. Darst. |
| ISBN: | 0444805915 0444805745 |
Staff View
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| 245 | 1 | 0 | |a A guidebook to lipoprotein technique |c G. L. Mills and Patricia A. Lane and P. K. Weech |
| 264 | 1 | |a Amsterdam u.a. |b Elsevier |c 1984 | |
| 300 | |a XIV, 512 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
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| 490 | 1 | |a Laboratory techniques in biochemistry and molecular biology |v 14 | |
| 500 | |a Literaturverz. S. 491 - 507 | ||
| 650 | 7 | |a Lipoproteins |2 cabt | |
| 650 | 7 | |a Techniques |2 cabt | |
| 650 | 4 | |a Lipoprotéines sanguines - Manuels de laboratoire | |
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| 650 | 4 | |a Blood lipoproteins |v Laboratory manuals | |
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| adam_text | IMAGE 1
CONTENTS
PREFACE V
GLOSSARY OF LIPOPROTEIN NOMENCLATURE AS USED IN THIS MANUAL . VII
GLOSSARY OF APO-LIPOPROTEIN NOMENCLATURE AS USED IN THIS MANUAL VIII
CHAPTER 1. BRIEF INTRODUCTION TO THE PLASMA LIPOPROTEINS . .. 1
1.1. NOMENCLATURE OF THE LIPOPROTEINS 6
1.1.1. ELECTROPHORETIC MOBILITY 7
1.1.2. HYDRATED DENSITY 7
1.1.3. FLOTATION RATE 10
1.1.4. PARTICLE SIZE 11
1.1.5 11
1.1.6. LIPOPROTEINS NAMED SPECIFICALLY 13
1.2. NOMENCLATURE OF APO-LIPOPROTEINS 13
CHAPTER 2. THE ISOLATION AND PURIFICATION OF PLASMA LIPOPROTEINS 18
2.1. ISOLATION BY ULTRACENTRIFUGATION . 20
2.1.1. SERIAL ULTRACENTRIFUGATION 25
2.1.1.1. PREPARATION OF SPECIFIC DILUENTS 26
2.1.1.2. ADJUSTMENT OF DENSITY WITH SOLID SALTS 36
2.1.1.3. MEASUREMENT AND FINAL ADJUSTMENT OF DENSITY 38
2.1.1.4. CHOICE OF ROTOR AND TUBES 43
2.1.1.5. LOADING THE TUBES 47
2.1.1.6. LOADING THE ROTOR 49
IX
IMAGE 2
X A GUIDEBOOK TO LIPOPROTEIN TECHNIQUE
2.1.1.7. UNLOADING THE ROTOR 50
2.1.1.8. SPECIMEN PROCEDURE FOR SERIAL CENTRIFUGATION 55
2.1.1.9. SUBFRACTIONATION OF CHYLOMICRONS AND VLDL 60
2.1.1.10. SERIAL CENTRIFUGATION AT DIFFERENT TEMPERATURES 64
2.1.2. DENSITY GRADIENT CENTRIFUGATION 64
2.1.2.1. CHOICE OF ROTOR AND TUBES 69
2.1.2.2. MAKING THE GRADIENT 70
2.1.2.3. LOADING AND UNLOADING THE ROTOR 73
2.1.2.4. UNLOADING THE GRADIENT 74
2.1.2.5. THE PROFILE OF THE GRADIENT 75
2.1.2.6. RATE-ZONAL ULTRACENTRIFUGATION 77
2.2. ISOLATION BY PRECIPITATION 78
2.2.1. CHOICE OF METHOD 85
2.2.2. RE-SOLUTION OF PRECIPITATED LIPOPROTEINS 87
2.2.3. PRECIPITATION WITH POLYANIONS 87
2.2.3.1. PRECIPITATION OF SS- ANCT~PRE-SS-LIPOPROTEINS WITH HEPARIN . . ..
88 2.2.3.2. PRECIPITATION OF A- AND SS-LIPOPROTEINS WITH SODIUM
PHOSPHOTUNG- STATE 90
2.2.3.3. PRECIPITATION OF A- AND SS-LIPOPROTEINS WITH DEXTRAN SULPHATE .
. 91 2.2.4. PROCEDURE FOR LOW-TEMPERATURE ETHANOL PRECIPITATION 93
2.3. ISOLATION BY CHROMATOGRAPHY 94
2.3.1. GEL FILTRATION CHROMATOGRAPHY 95
2.3.2. AFFINITY CHROMATOGRAPHY 98
2.3.2.1. IMMUNOADSORBENTS 98
2.3.2.2. IMMOBILISED HEPARIN 101
2.3.2.3. IMMOBILISED LECTIN 103
2.3.3. ADSORPTION CHROMATOGRAPHY 105
2.4. ISOLATION BY PREPARATIVE ELECTROPHORESIS 106
2.5. THE ISOLATION OF LP(A) AND LP-X 109
2.5.1. LP(A) 110
2.5.2. LP-X 112
2.6. THE PRESERVATION OF ISOLATED LIPOPROTEINS 114
CHAPTER 3. THE CHARACTERISATION OF PLASMA LIPOPROTEINS: INTRO- DUCTION
TO PRINCIPLES AND METHODS 117
3.1. THE PURITY OF LIPOPROTEIN PREPARATIONS 117
3.2. PHYSICAL AND CHEMICAL CHARACTERISATION 118
3.2.1. PHYSICAL METHODS 119
3.2.2. CHEMICAL CHARACTERISATION 122
3.3. HETEROGENEITY AND POLYDISPERSITY 125
3.3.1. CONTAMINATION WITH LIPOPROTEINS THAT LIE OUTSIDE THE NOMINAL
BOUNDS OF THE PREPARATION 128
IMAGE 3
CONTENTS XI
3.3.2. HETEROGENEITY WITHIN THE NOMINAL BOUNDS OF THE PREPARATION . . ..
129
3.3.2.1. ELECTRON MICROSCOPY 129
3.3.2.2. ULTRACENTRIFUGATION 130
3.3.2.3. GEL FILTRATION CHROMATOGRAPHY 133
3.3.2.4. ADSORPTION CHROMATOGRAPHY 134
3.3.2.5. ELECTROPHORESIS 135
CHAPTER 4. PHYSICAL CHARACTERISATION OF LIPOPROTEINS 139
4.1. THE ANALYTICAL ULTRACENTRIFUGE 139
4.1.1. ESTIMATION OF POLYDISPERSITY IN THE ANALYTICAL ULTRACENTRIFUGE .
. .. 141
4.1.2. SEDIMENTATION RATE, PARTICLE WEIGHT A ND PARTICLE DIAMETER 145
4.1.2.1. MEASUREMENT OF FLOTATION (SEDIMENTATION) RATE 148
4.1.2.2. MEASUREMENT OF PARTICLE WEIGHT AND DIAMETER 149
4.2. PARTIAL SPECIFIC VOLUME (HYDRATED DENSITY) 153
4.3. THE ANALYSIS OF POLYDISPERSITY IN THE PREPARATIVE ULTRACENTRIFUGE .
. .. 156
4.3.1. EQUILIBRIUM CENTRIFUGATION 160
4.4. GEL FILTRATION 163
4.4.1. THE ESTIMATION OF POLYDISPERSITY BY GEL FILTRATION CHROMATOGRAPHY
. 165 4.5. ELECTRON MICROSCOPY 166
CHAPTER 5. ELECTROPHORETIC ANALYSIS OF INTACT LIPOPROTEINS . . .171
5.1. PREPARATION OF SAMPLES 173
5.2. ELECTROPHORESIS ON PAPER 174
5.3. ELECTROPHORESIS ON CELLULOSE ACETATE MEMBRANES 178
5.4. ELECTROPHORESIS IN AGAROSE GEL 180
5.5. ELECTROPHORESIS IN POLYACRYLAMIDE GEL 185
5.5.1. PREPARATION OF ACRYLAMIDE GELS 187
5.5.2. STAINING PROCEDURES 192
5.5.3. ACRYLAMIDE GELS FOR LIPOPROTEIN ANALYSIS 194
5.5.3.1. GEL OF UNIFORM PORE SIZE 194
5.5.3.2. DISCONTINUOUS SEPARATING GELS 196
5.5.3.3. POLYACRYLAMIDE GRADIENTS GELS 198
5.6. IMMUNOELECTROPHORESIS 202
5.6.1. ELECTROPHORESIS OF LIPOPROTEIN-X (LP-X) 203
5.7. TWO-DIMENSIAL IMMUNO-ELECTROPHORESIS 210
5.8. ISOELECTRIC FOCUSSING 219
IMAGE 4
XUE A GUIDEBOOK TO L I P O P R O T E IN T E C H N I Q UE
CHAPTER 6. THE CHEMICAL CHARACTERISATION OF LIPOPROTEINS . . . 221
6.1. ESTIMATION OF TOTAL LIPOPROTEIN 224
6.1.1. ESTIMATION OF LIPOPROTEIN BY REFRACTORAETRY 226
6.2. SEPARATION OF THE LIPID AND PROTEIN MOIETIES 228
6.3. ANALYSIS OF THE LIPID MOIETY 228
6.3.1. CHROMATOGRAPHIE SEPARATION OF LIPIDS 229
6.3.1.1. CHROMATOGRAPHY ON COLUMNS OF SILICIC ACID 230
6.3.1.2. THIN-LAYER CHROMATOGRAPHY 231
6.3.1.3. GAS CHROMATOGRAPHY 235
6.3.2. ESTIMATION OF LIPIDS 236
6.3.2.1. CHOLESTEROL 237
6.3.2.2. GLYCERIDES 238
6.3.2.3. PHOSPHORUS 240
6.3.2.4. FATTY ACID ANALYSIS 241
6.3.2.5. DETERMINATION OF UNUSUAL COMPONENTS 242
6.4. ANALYSIS OF THE PROTEIN MOIETY 242
6.4.1. QUANTITATIVE ESTIMATION OF TOTAL APO-LIPOPROTEINS 246
6.4.1.1. TOTAL PROTEIN 247
6.4.1.2. CHEMICAL ESTIMATION OF APO-LIPOPROTEIN B 249
6.4.2. ELECTROPHORETIC ANALYSIS OF APO-LIPOPROTEINS 252
6.4.2.1. TECHNIQUES FOR STAINING GELS 253
6.4.2.2. RECORDING THE ELECTROPHORETIC PATTERNS 258
6.4.2.3. THE POLYACRYLAMIDE GEL-UREA SYSTEM 259
6.4.2.4. THE POLYACRYLAMIDE GEL-SODIUM DODECYL SULPHATE (SDS) SYSTEM .
266 6.4.2.5. ESTIMATION OF MOBILITY AND MOLECULAR WEIGHT BY SDS-GEL
ELECTRO- PHORESIS 270
6.4.2.6. ISOELECTRIC FOCUSSING (IEF) IN POLYACRYLAMIDE GEL 273
6.4.3. THE IDENTIFICATION OF APO-LIPOPROTEINS BY ELECTROPHORETIC
ANALYSIS . . 276 6.5. QUALITATIVE ANALYSIS OF APO-LIPOPROTEINS BY
IMMUNODIFFUSION 288 6.6. IDENTIFICATION AND ESTIMATION OF CARBOHYDRATES
295
6.7. QUANTITATIVE ANALYSIS OF LIPOPROTEINS BY MICRO-SCALE THIN-LAYER
CHROMATOGRA- PHY 300
CHAPTER 7. THE ESTIMATION OF LIPOPROTEINS 308
7.1. ESTIMATION OF LIPOPROTEINS BY REFRACTOMETRY 312
7.1.1. ANALYTICAL ULTRACENTRIFUGATION 315
7.2. NEPHELOMETRY 351
7.3. THE ESTIMATION OF LIPOPROTEINS BY CHEMICAL METHODS 357
7.3.1. PRECIPITATION METHODS FOR HDL -CHOLESTEROL 358
7.3.2. THE ESTIMATION OF LDL- AND VLDL-CHOLESTEROL 363
7.3.3. DERIVED ESTIMATES OF LIPOPROTEIN CONCENTRATION 366
IMAGE 5
CONTENTS XUL
7.3.4. QUANTIFICATION OF ELECTROPHORETIC ANALYSES 367
7.3.4.1. ELUTION OF LIPIDS 367
7.3.4.2. STAINING AND PHOTOMETRIC ESTIMATION 368
7.3.4.3. THE HPOPROTEINS ARE PRECIPITATED IN THE AGAROSE GEL WITH
POLYANIONS 376 7.4. ESTIMATION OF LP-X 378
7.5. IMMUNOASSAY OF LIPOPROTEINS 380
CHAPTER 8. ISOLATION AND ASSAY OF APO-LIPOPROTEINS 384
8.1. ISOLATION OF APO-LIPOPROTEIN A-L 394
8.1.1. CHROMATOGRAPHIE PROCEDURE 394
8.1.2. ISOELECTRIC FOCUSSING 399
8.2. ISOLATION OF APO-LIPOPROTEIN A-II 403
8.3. ISOLATION OF APO-LIPOPROTEIN A-IV 404
8.4. ISOLATION OF APO-LIPOPROTEIN B 405
8.4.1. CHROMATOGRAPHIE PROCEDURE 406
8.4.2. NON-OXIDATIVE PROCEDURE 408
8.5. ISOLATION OF THE C APO-LIPOPROTEINS 410
8.5.1. CHROMATOGRAPHIE PROCEDURE 412
8.5.2. ISOELECTRIC FOCUSSING 414
8.6. ISOLATION OF APO-LIPOPROTEIN D 416
8.7. ISOLATION OF APO-LIPOPROTEIN E 418
8.8. ISOLATION OF APO-LIPOPROTEIN F 421
8.9. THE ESTIMATION OF APO-LIPOPROTEINS 421
8.9.1. APO-LIPOPROTEIN ASSAY BY ELECTROPHORESIS 435
8.9.2. RADIAL IMMUNO-DIFFUSION 438
8.9.3. ELECTRO-IMMUNOASSAY 440
8.9.4. RADIO-IMMUNOASSAY 445
APPENDIX I. THE COLLECTION AND PRESERVATION OF BLOOD PLASMA 449
APPENDIX 2. METHODS OF CONCENTRATING LIPOPROTEIN SOLUTIONS . 460
APPENDIX 3. THE EXTRACTION OF LIPID FROM LIPOPROTEINS . . .. 463
APPENDIX 4. THE PREPARATION OF STAINS 472
IMAGE 6
XIV A GUIDEBOOK TO L I P O P R O T E IN T E C H N I Q UE
APPENDIX 5. THE PHOTOGRAPHY OF ELECTROPHORESIS AND IMMUNO- DIFFUSION
GELS 479
APPENDIX 6. RADIO-LABELLING 482
LIST OF SUPPLIERS MENTIONED IN THE TEXT 488
REFERENCES 491
SUBJECT INDEX 508
|
| any_adam_object | 1 |
| author | Mills, Gervase L. Lane, Patricia A. Weech, Philip K. |
| author_facet | Mills, Gervase L. Lane, Patricia A. Weech, Philip K. |
| author_role | aut aut aut |
| author_sort | Mills, Gervase L. |
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| building | Verbundindex |
| bvnumber | BV000970661 |
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| callnumber-label | QD415 |
| callnumber-raw | QD415.5 QP519 |
| callnumber-search | QD415.5 QP519 |
| callnumber-sort | QD 3415.5 |
| callnumber-subject | QD - Chemistry |
| classification_rvk | WC 4150 |
| ctrlnum | (OCoLC)10710912 (DE-599)BVBBV000970661 |
| dewey-full | 574.19/2/028 612/.12 |
| dewey-hundreds | 500 - Natural sciences and mathematics 600 - Technology (Applied sciences) |
| dewey-ones | 574 - [Unassigned] 612 - Human physiology |
| dewey-raw | 574.19/2/028 612/.12 |
| dewey-search | 574.19/2/028 612/.12 |
| dewey-sort | 3574.19 12 228 |
| dewey-tens | 570 - Biology 610 - Medicine and health |
| discipline | Biologie Medizin |
| format | Book |
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| id | DE-604.BV000970661 |
| illustrated | Illustrated |
| indexdate | 2024-07-09T15:21:58Z |
| institution | BVB |
| isbn | 0444805915 0444805745 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-000589907 |
| oclc_num | 10710912 |
| open_access_boolean | |
| owner | DE-19 DE-BY-UBM DE-355 DE-BY-UBR DE-12 DE-703 DE-83 DE-188 |
| owner_facet | DE-19 DE-BY-UBM DE-355 DE-BY-UBR DE-12 DE-703 DE-83 DE-188 |
| physical | XIV, 512 S. Ill., graph. Darst. |
| publishDate | 1984 |
| publishDateSearch | 1984 |
| publishDateSort | 1984 |
| publisher | Elsevier |
| record_format | marc |
| series | Laboratory techniques in biochemistry and molecular biology |
| series2 | Laboratory techniques in biochemistry and molecular biology |
| spelling | Mills, Gervase L. Verfasser aut A guidebook to lipoprotein technique G. L. Mills and Patricia A. Lane and P. K. Weech Amsterdam u.a. Elsevier 1984 XIV, 512 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Laboratory techniques in biochemistry and molecular biology 14 Literaturverz. S. 491 - 507 Lipoproteins cabt Techniques cabt Lipoprotéines sanguines - Manuels de laboratoire Lipoprotéines sanguines ram Blood lipoproteins Laboratory manuals Lipoproteins blood Laboratory Manuals Lipoproteide (DE-588)4074259-3 gnd rswk-swf Chemische Analyse (DE-588)4009840-0 gnd rswk-swf Serumlipoproteine (DE-588)4181061-2 gnd rswk-swf Labormedizin (DE-588)4073931-4 gnd rswk-swf Lipoproteide (DE-588)4074259-3 s DE-604 Chemische Analyse (DE-588)4009840-0 s Serumlipoproteine (DE-588)4181061-2 s Labormedizin (DE-588)4073931-4 s Lane, Patricia A. Verfasser aut Weech, Philip K. Verfasser aut Laboratory techniques in biochemistry and molecular biology 14 (DE-604)BV005871299 14. SWB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=000589907&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Mills, Gervase L. Lane, Patricia A. Weech, Philip K. A guidebook to lipoprotein technique Laboratory techniques in biochemistry and molecular biology Lipoproteins cabt Techniques cabt Lipoprotéines sanguines - Manuels de laboratoire Lipoprotéines sanguines ram Blood lipoproteins Laboratory manuals Lipoproteins blood Laboratory Manuals Lipoproteide (DE-588)4074259-3 gnd Chemische Analyse (DE-588)4009840-0 gnd Serumlipoproteine (DE-588)4181061-2 gnd Labormedizin (DE-588)4073931-4 gnd |
| subject_GND | (DE-588)4074259-3 (DE-588)4009840-0 (DE-588)4181061-2 (DE-588)4073931-4 |
| title | A guidebook to lipoprotein technique |
| title_auth | A guidebook to lipoprotein technique |
| title_exact_search | A guidebook to lipoprotein technique |
| title_full | A guidebook to lipoprotein technique G. L. Mills and Patricia A. Lane and P. K. Weech |
| title_fullStr | A guidebook to lipoprotein technique G. L. Mills and Patricia A. Lane and P. K. Weech |
| title_full_unstemmed | A guidebook to lipoprotein technique G. L. Mills and Patricia A. Lane and P. K. Weech |
| title_short | A guidebook to lipoprotein technique |
| title_sort | a guidebook to lipoprotein technique |
| topic | Lipoproteins cabt Techniques cabt Lipoprotéines sanguines - Manuels de laboratoire Lipoprotéines sanguines ram Blood lipoproteins Laboratory manuals Lipoproteins blood Laboratory Manuals Lipoproteide (DE-588)4074259-3 gnd Chemische Analyse (DE-588)4009840-0 gnd Serumlipoproteine (DE-588)4181061-2 gnd Labormedizin (DE-588)4073931-4 gnd |
| topic_facet | Lipoproteins Techniques Lipoprotéines sanguines - Manuels de laboratoire Lipoprotéines sanguines Blood lipoproteins Laboratory manuals Lipoproteins blood Laboratory Manuals Lipoproteide Chemische Analyse Serumlipoproteine Labormedizin |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=000589907&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| volume_link | (DE-604)BV005871299 |
| work_keys_str_mv | AT millsgervasel aguidebooktolipoproteintechnique AT lanepatriciaa aguidebooktolipoproteintechnique AT weechphilipk aguidebooktolipoproteintechnique |