Electrophoresis of proteins in polyacrylamide and starch gels:
Saved in:
| Main Author: | |
|---|---|
| Format: | Book |
| Language: | English |
| Published: |
Amsterdam
North-Holland
1973
|
| Edition: | 5. print. |
| Series: | Laboratory techniques in biochemistry and molecular biology. Paperback edition
1,1 |
| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Physical Description: | 149 S. Ill., graph. Darst. |
Staff View
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| 245 | 1 | 0 | |a Electrophoresis of proteins in polyacrylamide and starch gels |
| 250 | |a 5. print. | ||
| 264 | 1 | |a Amsterdam |b North-Holland |c 1973 | |
| 300 | |a 149 S. |b Ill., graph. Darst. | ||
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| 337 | |b n |2 rdamedia | ||
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| 490 | 1 | |a Laboratory techniques in biochemistry and molecular biology. Paperback edition |v 1,1 | |
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Record in the Search Index
| _version_ | 1804115364663001088 |
|---|---|
| adam_text | Contents
Chapter 1. Applicability of starch and acrylamide zone electro
phoresis 7
Introduction 7
Analytical applications 7
Preparative applications 8
Properties of starch and acrylamide gels 9
Movement of fluid through gels 13
Adsorption effects band shapes 14
Mechanical properties 16
Transparency 18
Gels suitable for separation on the basis of charge difference alone .... 18
Buffers suitable for electrophoresis in gels 19
The same buffer in the gel and in the electrode vessels 20
Different buffers in the gel and in the electrode chambers 23
Exchange of buffer after gel has been set 26
Gels containing solubilisers 26
Urea 26
Sodium dodecyl sulphate 27
Phenol and acetic acid 28
Evolution of heat and permissible thickness of gels 28
Chapter 2. Acrylamide gel 34
Choice of gel 34
Choice of buffer solutions 38
Acrylamide gel rendered soluble after use 42
4 ELECTROPHORESIS OF PROTEINS IN GELS
Catalysts 43
Proportion of bisacrylamide to total acrylamide 45
Chapter 3. Apparatus and techniques 46
Choice of apparatus 46
Apparatus suitable for gels which are to be stained 46
Optimum conditions for electrophoresis in vertical glass tubes; disc electro
phoresis 50
Preparation of gel (an example) 52
Electrode buffer 53
Sample solution . . . : 53
Addition of sample solution 54
Time and current required for electrophoresis 54
Removal of gel cylinders from glass tubes 54
Use of longer gels suitable for disintegration by extrusion 55
Staining and destaining 55
Vertical slab between cooling plates 56
Preparation of gel and leakage check 56
Entry ofproteins into gels by preliminary passage of current 57
Sample insertion 57
Possible reasons for distortion of bands 59
Voltage, current and time of electrophoresis 59
Two dimensional techniques 60
Staining methods 61
Spectrophotometry of acrylamide gels 64
Drying and storage of stained acrylamide gels 66
Autoradiographs 67
Estimation of radioactivity in acrylamide gel 69
Chapter 4. Methods allowing recovery of separated materials (pre¬
parative methods) 73
Advantages and disadvantages of acrylamide gels 73
Insolubility 73
Flexibility and resistance to cutting 74
Resistance to disintegration 75
Stickiness and adherence to glass 75
Swelling 76
Formation of gels with a flat upper surface 76
Methods of employment and types of apparatus 77
Elution from sections of gel before or after disintegration 77
CONTENTS 5
Vertical columns with end elution 78
Shape and degree of separation of peaks obtained by continuous elution. 79
Apparatus of Gordon and Louis 81
Apparatus of Hjerten et al 86
Apparatus of Duesberg and Rueckert 89
Apparatus of Brownstone 90
Chapter 5. Starch gel 93
Introduction 93
Pretreatment of granular starch in acid acetone 93
Choice of appropriate starch gel 95
Pore size 95
Buffer solution in the gel 102
Effect of urea when present in starch gel 105
Buffer systems for particular proteins (starch gel) 106
Preparation of starch gel 107
Choice of apparatus 110
Horizontal apparatus (normal size) Ill
Horizontal apparatus (micro scale) 113
Vertical apparatus 117
Separations in two dimensions 118
Two dimensional apparatus 120
Treatment of starch gel after electrophoresis 120
Slicing 120
Staining 121
Densitometry 123
Autoradiographs 127
Special adaptations for recovery of materials after separation in starch gel . 127
Removal of soluble starch 132
Acknowledgements 132
Appendix 133
References 140
Subject index 145
|
| any_adam_object | 1 |
| author | Gordon, Arthur H. 1916- |
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| bvnumber | BV000931442 |
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| classification_rvk | WC 3460 WC 4150 |
| ctrlnum | (OCoLC)2053490 (DE-599)BVBBV000931442 |
| discipline | Biologie |
| edition | 5. print. |
| format | Book |
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| id | DE-604.BV000931442 |
| illustrated | Illustrated |
| indexdate | 2024-07-09T15:21:27Z |
| institution | BVB |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-000570587 |
| oclc_num | 2053490 |
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| owner | DE-19 DE-BY-UBM |
| owner_facet | DE-19 DE-BY-UBM |
| physical | 149 S. Ill., graph. Darst. |
| publishDate | 1973 |
| publishDateSearch | 1973 |
| publishDateSort | 1973 |
| publisher | North-Holland |
| record_format | marc |
| series | Laboratory techniques in biochemistry and molecular biology. Paperback edition |
| series2 | Laboratory techniques in biochemistry and molecular biology. Paperback edition |
| spelling | Gordon, Arthur H. 1916- Verfasser (DE-588)140456562 aut Electrophoresis of proteins in polyacrylamide and starch gels 5. print. Amsterdam North-Holland 1973 149 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Laboratory techniques in biochemistry and molecular biology. Paperback edition 1,1 Electrophoresis Disc-Elektrophorese (DE-588)4328776-1 gnd rswk-swf Proteine (DE-588)4076388-2 gnd rswk-swf Elektrophorese (DE-588)4014373-9 gnd rswk-swf Gelelektrophorese (DE-588)4123267-7 gnd rswk-swf Stärke (DE-588)4182793-4 gnd rswk-swf Proteine (DE-588)4076388-2 s Elektrophorese (DE-588)4014373-9 s DE-604 Gelelektrophorese (DE-588)4123267-7 s Stärke (DE-588)4182793-4 s 1\p DE-604 Disc-Elektrophorese (DE-588)4328776-1 s 2\p DE-604 Laboratory techniques in biochemistry and molecular biology. Paperback edition 1,1 (DE-604)BV005871299 1,1 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=000570587&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis 1\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk 2\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk |
| spellingShingle | Gordon, Arthur H. 1916- Electrophoresis of proteins in polyacrylamide and starch gels Laboratory techniques in biochemistry and molecular biology. Paperback edition Electrophoresis Disc-Elektrophorese (DE-588)4328776-1 gnd Proteine (DE-588)4076388-2 gnd Elektrophorese (DE-588)4014373-9 gnd Gelelektrophorese (DE-588)4123267-7 gnd Stärke (DE-588)4182793-4 gnd |
| subject_GND | (DE-588)4328776-1 (DE-588)4076388-2 (DE-588)4014373-9 (DE-588)4123267-7 (DE-588)4182793-4 |
| title | Electrophoresis of proteins in polyacrylamide and starch gels |
| title_auth | Electrophoresis of proteins in polyacrylamide and starch gels |
| title_exact_search | Electrophoresis of proteins in polyacrylamide and starch gels |
| title_full | Electrophoresis of proteins in polyacrylamide and starch gels |
| title_fullStr | Electrophoresis of proteins in polyacrylamide and starch gels |
| title_full_unstemmed | Electrophoresis of proteins in polyacrylamide and starch gels |
| title_short | Electrophoresis of proteins in polyacrylamide and starch gels |
| title_sort | electrophoresis of proteins in polyacrylamide and starch gels |
| topic | Electrophoresis Disc-Elektrophorese (DE-588)4328776-1 gnd Proteine (DE-588)4076388-2 gnd Elektrophorese (DE-588)4014373-9 gnd Gelelektrophorese (DE-588)4123267-7 gnd Stärke (DE-588)4182793-4 gnd |
| topic_facet | Electrophoresis Disc-Elektrophorese Proteine Elektrophorese Gelelektrophorese Stärke |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=000570587&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| volume_link | (DE-604)BV005871299 |
| work_keys_str_mv | AT gordonarthurh electrophoresisofproteinsinpolyacrylamideandstarchgels |