Fundamentals of light microscopy:
Saved in:
| Main Author: | |
|---|---|
| Format: | Book |
| Language: | English |
| Published: |
Cambridge u.a.
Cambridge Univ. Pr.
1982
|
| Edition: | 1. publ. |
| Series: | International Union for Pure and Applied Biophysics: IUPAB biophysics series
6 |
| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Physical Description: | X, 93 S. Ill., graph. Darst. |
| ISBN: | 0521247942 052128967X |
Staff View
MARC
| LEADER | 00000nam a2200000 cb4500 | ||
|---|---|---|---|
| 001 | BV000030120 | ||
| 003 | DE-604 | ||
| 005 | 20010607 | ||
| 007 | t | ||
| 008 | 870612s1982 ad|| |||| 00||| eng d | ||
| 020 | |a 0521247942 |9 0-521-24794-2 | ||
| 020 | |a 052128967X |9 0-521-28967-X | ||
| 035 | |a (OCoLC)8170657 | ||
| 035 | |a (DE-599)BVBBV000030120 | ||
| 040 | |a DE-604 |b ger |e rakddb | ||
| 041 | 0 | |a eng | |
| 049 | |a DE-12 |a DE-188 | ||
| 050 | 0 | |a QH205.2 | |
| 082 | 0 | |a 502/.8/2 |2 19 | |
| 100 | 1 | |a Spencer, Michael |e Verfasser |4 aut | |
| 245 | 1 | 0 | |a Fundamentals of light microscopy |
| 250 | |a 1. publ. | ||
| 264 | 1 | |a Cambridge u.a. |b Cambridge Univ. Pr. |c 1982 | |
| 300 | |a X, 93 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 490 | 1 | |a International Union for Pure and Applied Biophysics: IUPAB biophysics series |v 6 | |
| 650 | 4 | |a Microscopie | |
| 650 | 4 | |a Microscopy | |
| 650 | 0 | 7 | |a Mikroskopie |0 (DE-588)4039238-7 |2 gnd |9 rswk-swf |
| 689 | 0 | 0 | |a Mikroskopie |0 (DE-588)4039238-7 |D s |
| 689 | 0 | |5 DE-604 | |
| 830 | 0 | |a International Union for Pure and Applied Biophysics: IUPAB biophysics series |v 6 |w (DE-604)BV000001215 |9 6 | |
| 856 | 4 | 2 | |m SWB Datenaustausch |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=000004238&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
| 999 | |a oai:aleph.bib-bvb.de:BVB01-000004238 | ||
Record in the Search Index
| _version_ | 1804114484574289920 |
|---|---|
| adam_text | IMAGE 1
CONTENTS
PREFACE IX
1 MICROSCOPE ALIGNMENT 1
BASIC GEOMETRICAL OPTICS 1
KOHLER ILLUMINATION 4
ALIGNMENT PROCEDURE 6
LOOKING AFTER THE MICROSCOPE 7
QUESTIONS 8
2 PROPERTIES OF LIGHT 9
BASIC PRINCIPLES - WHY BOTHER TO LEARN THEM? 9
PROPERTIES OF WAVES 10
WAVE INTERFERENCE 13
QUESTIONS 16
3 THE LIMITS OF RESOLUTION 18
THE RAYLEIGH CRITERION 21
LIMITS TO MAGNIFICATION 22
COMPARISONS WITH ELECTRON MICROSCOPY 23
QUESTIONS 23
4 THE ABBE APPROACH 24
FOURIER ANALYSIS 24
THE RESOLUTION LIMIT 27
OPTICAL AND X-RAY DIFFRACTION 29
QUESTIONS 31
5 BRIGHT-FIELD, PHASE AND DARK-FIELD MICROSCOPY 32
PHASE CONTRAST 33
ALIGNMENT OF THE PHASE MICROSCOPE 36
DARK-FIELD MICROSCOPY 37
QUESTIONS 38
IMAGE 2
CONTENTS
6 FLUORESCENCE MICROSCOPY 40
THE NATURE OF FLUORESCENCE 40
TYPES OF WAVELENGTH FILTER 41
OPTICAL SYSTEMS FOR OBSERVING FLUORESCENCE 42
IMMUNOFLUORESCENCE 43
OTHER TECHNIQUES 44
QUESTIONS 45
7 POLARIZING MICROSCOPY 46
THE PRODUCTION OF POLARIZED LIGHT 46
BIREFRINGENCE 48
THE MEASUREMENT OF BIREFRINGENCE 50
QUESTIONS 53
8 INTERFERENCE METHODS 54
INTERFERENCE CONTRAST 54
THE INTERFEROMETER MICROSCOPE 56
THE MEASUREMENT OF MASS OR THICKNESS 59
INTERFERENCE REFLECTION MICROSCOPY 60
TOTAL INTERNAL REFLECTION MICROSCOPY 61
QUESTIONS 61
9 ABERRATIONS AND MICROSCOPE DESIGN 63
CHROMATIC ABERRATION 63
SPHERICAL ABERRATION 64
OTHER ABERRATIONS 65
OBJECTIVE TESTING 66
MICROSCOPE COMPONENT MARKINGS 67
QUESTIONS 69
10 PHOTOMICROGRAPHY 70
FOCUSING THE IMAGE 70
CHOICE OF FILM AND EXPOSURE TIME 71
FACTORS AFFECTING IMAGE QUALITY 72
11 SUMMARY, CLASS EXPERIMENTS AND FURTHER READING 73
CLASS EXPERIMENTS 74
FURTHER READING 77
APPENDIX: MATHEMATICAL TREATMENTS 78
ANSWERS TO QUESTIONS 83
INDEX 87
|
| any_adam_object | 1 |
| author | Spencer, Michael |
| author_facet | Spencer, Michael |
| author_role | aut |
| author_sort | Spencer, Michael |
| author_variant | m s ms |
| building | Verbundindex |
| bvnumber | BV000030120 |
| callnumber-first | Q - Science |
| callnumber-label | QH205 |
| callnumber-raw | QH205.2 |
| callnumber-search | QH205.2 |
| callnumber-sort | QH 3205.2 |
| callnumber-subject | QH - Natural History and Biology |
| ctrlnum | (OCoLC)8170657 (DE-599)BVBBV000030120 |
| dewey-full | 502/.8/2 |
| dewey-hundreds | 500 - Natural sciences and mathematics |
| dewey-ones | 502 - Miscellany |
| dewey-raw | 502/.8/2 |
| dewey-search | 502/.8/2 |
| dewey-sort | 3502 18 12 |
| dewey-tens | 500 - Natural sciences and mathematics |
| discipline | Allgemeine Naturwissenschaft |
| edition | 1. publ. |
| format | Book |
| fullrecord | <?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01480nam a2200397 cb4500</leader><controlfield tag="001">BV000030120</controlfield><controlfield tag="003">DE-604</controlfield><controlfield tag="005">20010607 </controlfield><controlfield tag="007">t</controlfield><controlfield tag="008">870612s1982 ad|| |||| 00||| eng d</controlfield><datafield tag="020" ind1=" " ind2=" "><subfield code="a">0521247942</subfield><subfield code="9">0-521-24794-2</subfield></datafield><datafield tag="020" ind1=" " ind2=" "><subfield code="a">052128967X</subfield><subfield code="9">0-521-28967-X</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(OCoLC)8170657</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)BVBBV000030120</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-604</subfield><subfield code="b">ger</subfield><subfield code="e">rakddb</subfield></datafield><datafield tag="041" ind1="0" ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="049" ind1=" " ind2=" "><subfield code="a">DE-12</subfield><subfield code="a">DE-188</subfield></datafield><datafield tag="050" ind1=" " ind2="0"><subfield code="a">QH205.2</subfield></datafield><datafield tag="082" ind1="0" ind2=" "><subfield code="a">502/.8/2</subfield><subfield code="2">19</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Spencer, Michael</subfield><subfield code="e">Verfasser</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Fundamentals of light microscopy</subfield></datafield><datafield tag="250" ind1=" " ind2=" "><subfield code="a">1. publ.</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="a">Cambridge u.a.</subfield><subfield code="b">Cambridge Univ. Pr.</subfield><subfield code="c">1982</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">X, 93 S.</subfield><subfield code="b">Ill., graph. Darst.</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="b">n</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="b">nc</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="490" ind1="1" ind2=" "><subfield code="a">International Union for Pure and Applied Biophysics: IUPAB biophysics series</subfield><subfield code="v">6</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Microscopie</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Microscopy</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Mikroskopie</subfield><subfield code="0">(DE-588)4039238-7</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="689" ind1="0" ind2="0"><subfield code="a">Mikroskopie</subfield><subfield code="0">(DE-588)4039238-7</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2=" "><subfield code="5">DE-604</subfield></datafield><datafield tag="830" ind1=" " ind2="0"><subfield code="a">International Union for Pure and Applied Biophysics: IUPAB biophysics series</subfield><subfield code="v">6</subfield><subfield code="w">(DE-604)BV000001215</subfield><subfield code="9">6</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="m">SWB Datenaustausch</subfield><subfield code="q">application/pdf</subfield><subfield code="u">http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=000004238&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA</subfield><subfield code="3">Inhaltsverzeichnis</subfield></datafield><datafield tag="999" ind1=" " ind2=" "><subfield code="a">oai:aleph.bib-bvb.de:BVB01-000004238</subfield></datafield></record></collection> |
| id | DE-604.BV000030120 |
| illustrated | Illustrated |
| indexdate | 2024-07-09T15:07:27Z |
| institution | BVB |
| isbn | 0521247942 052128967X |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-000004238 |
| oclc_num | 8170657 |
| open_access_boolean | |
| owner | DE-12 DE-188 |
| owner_facet | DE-12 DE-188 |
| physical | X, 93 S. Ill., graph. Darst. |
| publishDate | 1982 |
| publishDateSearch | 1982 |
| publishDateSort | 1982 |
| publisher | Cambridge Univ. Pr. |
| record_format | marc |
| series | International Union for Pure and Applied Biophysics: IUPAB biophysics series |
| series2 | International Union for Pure and Applied Biophysics: IUPAB biophysics series |
| spelling | Spencer, Michael Verfasser aut Fundamentals of light microscopy 1. publ. Cambridge u.a. Cambridge Univ. Pr. 1982 X, 93 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier International Union for Pure and Applied Biophysics: IUPAB biophysics series 6 Microscopie Microscopy Mikroskopie (DE-588)4039238-7 gnd rswk-swf Mikroskopie (DE-588)4039238-7 s DE-604 International Union for Pure and Applied Biophysics: IUPAB biophysics series 6 (DE-604)BV000001215 6 SWB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=000004238&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Spencer, Michael Fundamentals of light microscopy International Union for Pure and Applied Biophysics: IUPAB biophysics series Microscopie Microscopy Mikroskopie (DE-588)4039238-7 gnd |
| subject_GND | (DE-588)4039238-7 |
| title | Fundamentals of light microscopy |
| title_auth | Fundamentals of light microscopy |
| title_exact_search | Fundamentals of light microscopy |
| title_full | Fundamentals of light microscopy |
| title_fullStr | Fundamentals of light microscopy |
| title_full_unstemmed | Fundamentals of light microscopy |
| title_short | Fundamentals of light microscopy |
| title_sort | fundamentals of light microscopy |
| topic | Microscopie Microscopy Mikroskopie (DE-588)4039238-7 gnd |
| topic_facet | Microscopie Microscopy Mikroskopie |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=000004238&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| volume_link | (DE-604)BV000001215 |
| work_keys_str_mv | AT spencermichael fundamentalsoflightmicroscopy |