Regulation of adipose tissue homeostasis by the death ligand TRAIL:
Gespeichert in:
| 1. Verfasser: | |
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| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
Ulm
[2018]
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| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis Inhaltsverzeichnis |
| Beschreibung: | 101 Seiten Illustrationen, Diagramme 30 cm |
Internformat
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| 245 | 1 | 0 | |a Regulation of adipose tissue homeostasis by the death ligand TRAIL |c Verena Zoller |
| 264 | 1 | |a Ulm |c [2018] | |
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Datensatz im Suchindex
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| adam_text | TABLE OF CONTENTS
LIST OF ABBREVIATIONS 6
1. INTRODUCTION 9
1.1 OBESITY 9
1.2 OBESITY AND ADIPOSE TISSUE 9
1.3 FUNCTION OF THE WAT 10
1.4 REGULATION OF ADIPOSE TISSUE MASS 12
1.4.1 PROLIFERATION 13
1.4.2 ADIPOGENIC DIFFERENTIATION 14
1.5 INFLAMMATION OF THE ADIPOSE TISSUE 15
1.6 APOPTOSIS IN ADIPOSE TISSUE 17
1.7 TRAIL 18
1.8 NON-APOPTOTIC FUNCTIONS OF DEATH LIGANDS IN ADIPOSE TISSUE 20
2. AIM OF THE STUDY 22
3. MATERIAL AND METHODS 23
3.1 MATERIALS 23
3.1.1 DEVICES 23
3.1.2 REAGENTS 23
3.1.3 OLIGONUCLEOTIDES 24
3.1.4 ANTIBODIES 26
3.1.5 ELISAS 27
3.1.6 ENZYMES 27
3.1.7 VECTORS 27
3.1.8 CELL CULTURE 27
3.2 METHODS 29
3.2.1 CELL CULTURE 29
3.2.2 DETERMINATION OF CELL NUMBER 30
3.2.3 PRIMARY HUMAN ADIPOSE STROMAL CELL ISOLATION 30
3.2.4 RNA ISOLATION AND CDNA PREPARATION 31
3.2.5 MRNA EXPRESSION ARRAYS 31
3.2.6 QUANTITATIVE REAL-TIME PCR (QPCR) 32
3.2.7 PROTEIN EXTRACTION 33
3.2.8 BRADFORD ASSAY 33
3.2.9 WESTERN BLOT 34
3.2.10 TRIGLYCERIDE ASSAY 34
3.2.11 LENTIVIRAL MEDIATED KNOCKDOWN OF CASPASE-8 35
3.2.12 CASPASE ACTIVITY ASSAY 35
3.2.13 NICOLETTI ASSAY 35
3.2.14 ELISA 36
3.2.15 ELECTROPHORETIC MOBILITY SHIFT ASSAY (EMSA) 36
3.2.16 REPORTER GENE ASSAY 38
3.2.17 STATISTICAL ANALYSIS
38
4. RESULTS 39
4.1 TRAIL INHIBITS ADIPOGENIC DIFFERENTIATION 39
4.1.1 TRAIL INHIBITS ADIPOGENIC DIFFERENTIATION IN HUMAN SGBS CELLS 39
4.1.2 TRAIL INHIBITS ADIPOGENIC DIFFERENTIATION IN HUMAN PRIMARY CELLS
40
4.1.3 THE INHIBITORY EFFECT OF TRAIL IS MEDIATED
VIA
TRAIL-R2 41
4.1.4 THE ANTI-ADIPOGENIC EFFECT OF TRAIL IS NOT MEDIATED
VIA
THE NON-CANONICAL TRAIL SIGNALING 42
4.1.4 CASPASES ARE RESPONSIBLE FOR THE INHIBITORY EFFECT OF TRAIL ON
ADIPOGENESIS 44
4.1.5 TRAIL BLOCKS THE UPREGULATION OF ADIPOGENIC TRANSCRIPTION FACTORS
47
4.2 TRAIL INDUCES A PRO-INFLAMMATORY EXPRESSION PROFILE IN ADIPOCYTES 49
4.2.1 TRAIL INDUCES A PRO-INFLAMMATORY EXPRESSION PROFILE IN
PREADIPOCYTES AND ADIPOCYTES 49
4.2.2 TRAIL INDUCES THE PRODUCTION OF IL-6, IL-8, MCP-1 AND CCL-20 IN A
TIME- AND DOSE-DEPENDENT MANNERSO
4.2.3 TRAIL INDUCES IL-6, IL-8 AND CCL-20 EXPRESSION IN HUMAN PRIMARY
ADIPOCYTES 52
4.2.4 CASPASES ARE NOT INVOLVED IN MEDIATING THE EFFECT OF TRAIL ON
CYTOKINE EXPRESSION 53
4.2.5 TRAIL INDUCES PHOSPHORYLATION OF IXBA 54
4.2.6 TRAIL INDUCES A PHOSPHORYLATION OF ERK1/2 58
5. DISCUSSION 61
5.1 USED TRAIL CONCENTRATIONS IN THIS STUDY 61
5.2 TRAIL INHIBITS ADIPOGENIC DIFFERENTIATION 61
5.2.2 MOLECULAR MECHANISM OF TRAIL IN REDUCING ADIPOGENIC
DIFFERENTIATION 62
5.2.3 NON-APOPTOTIC FUNCTIONS OF CASPASES 63
5.2.4 TRAIL INHIBITS IMPORTANT ADIPOGENIC TRANSCRIPTION FACTORS 64
5.3.1 MOLECULAR MECHANISM OF TRAIL IN MEDIATING AN INFLAMMATORY
SECRETION PROFILE 66
5.4 IMPLICATIONS OF THE STUDY 68
6. SUMMARY 73
REFERENCES 75
STATUTORY DECLARATION 98
ACKNOWLEDGEMENTS 99
CURRICULUM VITAE
100
|
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| discipline | Biologie Medizin |
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| spelling | Zoller, Verena 1987- Verfasser (DE-588)1160882703 aut Regulation of adipose tissue homeostasis by the death ligand TRAIL Verena Zoller Ulm [2018] 101 Seiten Illustrationen, Diagramme 30 cm txt rdacontent n rdamedia nc rdacarrier Dissertation Universität Ulm 2018 Fettsucht (DE-588)4016953-4 gnd rswk-swf Obesity. TNF-related apoptosis-inducing ligand. (DE-588)4113937-9 Hochschulschrift gnd-content Fettsucht (DE-588)4016953-4 s DE-604 Universität Ulm (DE-588)30607-1 dgg B:DE-101 application/pdf http://d-nb.info/1161872604/04 Inhaltsverzeichnis DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=030612786&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Zoller, Verena 1987- Regulation of adipose tissue homeostasis by the death ligand TRAIL Fettsucht (DE-588)4016953-4 gnd |
| subject_GND | (DE-588)4016953-4 (DE-588)4113937-9 |
| title | Regulation of adipose tissue homeostasis by the death ligand TRAIL |
| title_auth | Regulation of adipose tissue homeostasis by the death ligand TRAIL |
| title_exact_search | Regulation of adipose tissue homeostasis by the death ligand TRAIL |
| title_full | Regulation of adipose tissue homeostasis by the death ligand TRAIL Verena Zoller |
| title_fullStr | Regulation of adipose tissue homeostasis by the death ligand TRAIL Verena Zoller |
| title_full_unstemmed | Regulation of adipose tissue homeostasis by the death ligand TRAIL Verena Zoller |
| title_short | Regulation of adipose tissue homeostasis by the death ligand TRAIL |
| title_sort | regulation of adipose tissue homeostasis by the death ligand trail |
| topic | Fettsucht (DE-588)4016953-4 gnd |
| topic_facet | Fettsucht Hochschulschrift |
| url | http://d-nb.info/1161872604/04 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=030612786&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT zollerverena regulationofadiposetissuehomeostasisbythedeathligandtrail AT universitatulm regulationofadiposetissuehomeostasisbythedeathligandtrail |
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