Comparison of freshly isolated human hepatocytes with human hepatocyte-like cells:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
2003
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | Ulm, Univ., Diss., 2003 |
| Beschreibung: | 75 Bl. graph. Darst. : 30 cm |
Internformat
MARC
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| 100 | 1 | |a Yuan, Jing |d 1961- |e Verfasser |0 (DE-588)128836512 |4 aut | |
| 245 | 1 | 0 | |a Comparison of freshly isolated human hepatocytes with human hepatocyte-like cells |c vorgelegt von Jing Yuan |
| 264 | 1 | |c 2003 | |
| 300 | |a 75 Bl. |b graph. Darst. : 30 cm | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 500 | |a Ulm, Univ., Diss., 2003 | ||
| 655 | 7 | |0 (DE-588)4113937-9 |a Hochschulschrift |2 gnd-content | |
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| 943 | 1 | |a oai:aleph.bib-bvb.de:BVB01-012846630 | |
Datensatz im Suchindex
| _version_ | 1816445249804828672 |
|---|---|
| adam_text |
TABLE
OF
CONTENTS
ABBREVIATIONS
5
1
INTRODUCTION
7
1.1
GENERAL
ASPECTS
.
7
1.2
DRUG-METABOLIZING
ENZYMES
IN
THE
LIVER
.
8
1.2.1
PHASE
I
ENZYMES
AND
DRUG
METABOLISM
.
9
1.2.2
PHASE
II
ENZYMES
AND
DRUG
METABOLISM
.
12
1.3
THE
USE
OF
HUMAN
HEPATOCYTES
IN
DRUG
METABOLISM
.
13
1.4
AIMS
OF
THE
PRESENT
STUDY
.
14
2.
MATERIALS
AND
METHODS
15
2.1
ISOLATION
AND
CULTURE
OF
HUMAN
HEPATOCYTES
AND
NEOHEP
CELLS
.
15
2.1.1
HUMAN
HEPATOCYTE
ISOLATION
.
15
2.1.1.1
HUMAN
LIVER
TISSUE
PERFUSION
.
15
2.1.1.2
HUMAN
HEPATOCYTE
HARVEST
.
17
2.1.2
PRIMARY
HUMAN
HEPATOCYTE
CULTURE
.
19
2.2
NEOHEP
CELL
CULTURE
.
19
2.3
'
ASSAYS
OF
QUALITY
CONTROL
OF
PRIMARY
HUMAN
HEPATOCYTES
AND
NEOHEP
CELLS
.19
2.3.1
MEASUREMENT
OF
GENERAL
BIOCHEMICAL
PARAMETERS
.
19
2.3.1.1
LDH
MEASUREMENT
.
19
2.3.1.2
AST
MEASUREMENT
.
20
2.3.1.3
UREA
MEASUREMENT
.
22
2.3.1.4
GLUCOSE
MEASUREMENT
.
22
2.3.1.5
LACTATE
MEASUREMENT
.
23
2.3.2
ASSAYS
OF
SPECIFIC
FUNCTIONS
OF
PRIMARY
HUMAN
HEPATOCYTES
AND
NEOHEP
CELLS
.
24
2.3.2.1
ALBUMIN
MEASUREMENT
.
24
2.3.2.2
CYP1
AL/2
AND
CYP3A4
ENZYME
ACTIVITY
.
25
2.3.2.3
UDP-GLUCURONYLTRANSFERASES
(GT)
ACTIVITY
.
26
2.4
PREPARATION
OF
CELLULAR
PROTEIN
FOR
WESTERN
BLOT
ANALYSIS
AND
QUANTIFICATION.
27
2.4.1
PROTEIN
PREPARATION
AND
MEASUREMENT
.
27
2.4.2
DETECTION
OF
CYP
PROTEINS
BY
WESTERN
BLOT
ANALYSIS
.
29
2.5
TIME-DEPENDENT
VERAPAMIL
METABOLISM
IN
HUMAN
HEPATOCYTES
.
35
2.6
STATISTICAL
ANALYSIS
.
36
3
RESULTS
37
3.1
MORPHOLOGICAL
PRESENTATION
OF
HUMAN
HEPATOCYTES
AND
NEOHEP
CELLS
.
37
3.2
EFFECTS
OF
CULTURE
TIME
ON
TRANSAMINASE
MEMBRANE
LEAKAGE
IN
THE
INVESTIGATED
CELL
CULTURE
SYSTEMS
.
38
3.3
EFFECTS
OF
CULTURE
TIME
ON
GLUCOSE
CONSUMPTION
AND
LACTATE
FORMATION
IN
HUMAN
HEPATOCYTES
AND
NEOHEP
CELLS
.
40
3.4
EFFECTS
OF
CULTURE
TIME
ON
UREA
FORMATION
IN
HUMAN
HEPATOCYTES
AND
NEOHEP
CELLS
.
41
3.5
EFFECTS
OF
CULTURE
TIME
ON
ALBUMIN
SECRETION
IN
HUMAN
HEPATOCYTES
AND
NEOHEP
CELLS
.
42
3.6
EXPRESSIONS
OF
CYP
PROTEINS
IN
HUMAN
HEPATOCYTES
AND
NEOHEP
CELLS
.
43
3.6.1
CYP1A2
PROTEIN
EXPRESSION
IN
HUMAN
HEPATOCYTES
AND
NEOHEP
CELLS
.
44
3.6.2
CYP2D6
PROTEIN
EXPRESSION
IN
HUMAN
HEPATOCYTES
AND
NEOHEP
CELLS
.
44
3.6.3
CYP3A4
PROTEIN
EXPRESSION
IN
HUMAN
HEPATOCYTES
AND
NEOHEP
CELLS
.
45
3.7
EFFECTS
OF
CULTURE
TIME
ON
CYTOCHROME
P450
INDUCIBILITY
OF
CYP1A
AND
CYP3A
IN
HUMAN
HEPATOCYTES
AND
NEOHEP
CELLS
(PHASE
I)
.
47
3.7.
K
INDUCTION
OF
CYP1A
ENZYME
ACTIVITY
IN
HUMAN
HEPATOCYTES
AND
NEOHEP
CELLS
BY
OMEPRAZOL
AND
3-MC
.
47
3.7.2
INDUCTION
OF
CYP3A
ENZYME
ACTIVITY
IN
HUMAN
HEPATOCYTES
AND
NEOHEP
CELLS
BY
PHENOBARBITAL
AND
RIFAMPICIN
.
48
3.8
EFFECTS
OF
CULTURE
TIME
ON
UDPGT
ENZYME
ACTIVITY
IN
HUMAN
HEPATOCYTES
AND
NEOHEP
CELLS
(PHASE
II)
.
49
3.9
CYP
3A4-DEPENDENT
METABOLISM
OF
VERAPAMIL-HCL
IN
HUMAN
HEPATOCYTES
.
50
4
DISCUSSION
52
4.1
MICROSCOPICAL
AND
HISTOCHEMICAL
ANALYSIS
.
52
4.2
BIOCHEMICAL
PROPERTIES
.
53
4.3
CYTOCHROME
P450
PROTEIN
EXPRESSION
AND
ENZYME
ACTIVITY
.
55
4.4
PHASE
II
ENZYME
ACTIVITY
AND
DRUG
METABOLISM
.
57
4.5
CONCLUSION
.
58
5
SUMMARY
59
6
REFERENCES
61
7
ACKNOWLEDGMENTS
73
8
CURRICULUM
VITAE
74
4 |
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| indexdate | 2024-11-22T17:39:40Z |
| institution | BVB |
| language | English |
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| spelling | Yuan, Jing 1961- Verfasser (DE-588)128836512 aut Comparison of freshly isolated human hepatocytes with human hepatocyte-like cells vorgelegt von Jing Yuan 2003 75 Bl. graph. Darst. : 30 cm txt rdacontent n rdamedia nc rdacarrier Ulm, Univ., Diss., 2003 (DE-588)4113937-9 Hochschulschrift gnd-content DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=012846630&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Yuan, Jing 1961- Comparison of freshly isolated human hepatocytes with human hepatocyte-like cells |
| subject_GND | (DE-588)4113937-9 |
| title | Comparison of freshly isolated human hepatocytes with human hepatocyte-like cells |
| title_auth | Comparison of freshly isolated human hepatocytes with human hepatocyte-like cells |
| title_exact_search | Comparison of freshly isolated human hepatocytes with human hepatocyte-like cells |
| title_full | Comparison of freshly isolated human hepatocytes with human hepatocyte-like cells vorgelegt von Jing Yuan |
| title_fullStr | Comparison of freshly isolated human hepatocytes with human hepatocyte-like cells vorgelegt von Jing Yuan |
| title_full_unstemmed | Comparison of freshly isolated human hepatocytes with human hepatocyte-like cells vorgelegt von Jing Yuan |
| title_short | Comparison of freshly isolated human hepatocytes with human hepatocyte-like cells |
| title_sort | comparison of freshly isolated human hepatocytes with human hepatocyte like cells |
| topic_facet | Hochschulschrift |
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