The roles of semaphorin-3C in pericytes and endothelial cells:
Gespeichert in:
| 1. Verfasser: | |
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| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
[2013]
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| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | VIII, 97 S. Ill., graph. Darst. |
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Datensatz im Suchindex
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| adam_text | CONTENT
ZUSAMMENFASSUNG V
SUMMARY VII
1. INTRODUCTION 1
1.1 OVERVIEW OF THE VASCULAR
SYSTEM 1
1.1.1 BLOOD VESSEL FORMATION 2
1.1.2 SPROUTING ANGIOGENESIS 2
1.1.3 DELTA-NOTCH SIGNALING 4
1.1.4 VESSEL MATURATION AND QUIESCENCE 8
1.1.5 PATHOLOGICAL ANGIOGENESIS AND ANTI-ANGIOGENESIS TREATMENT 10
1.2 REGULATION
OF ANGIOGENESIS BY NEURAL
GUIDANCE MOLECULES 12
1.2.1 NEURAL GUIDANCE MOLECULES 13
1.2.2 CLASS-3 SEMAPHORIN SIGNALING 15
1.2.3 CLASS-3 SEMAPHORINS CONTROL PHYSIOLOGICAL AND PATHOLOGICAL
ANGIOGENESIS 17
1.2.4 THE BIOLOGICAL FUNCTIONS OF SEMAPHORIN-3C 20
1.3 AIM
OF THIS
STUDY 21
2. RESULTS 23
2.1
SEMA3C IS A NOVEL DOWNSTREAM TARGET
OF NOTCH
SIGNALING 23
2.1.1 SEMA3C IS A POTENTIAL TARGET OF TRANSCRIPTIONAL REPRESSORS HEYL
AND HEY2 23
2.1.2 SEMA3C IS REGULATED BY NOTCH SIGNALING IN HUMAN BRAIN PERICYTES 24
2.2 FUNCTIONAL ROLES
OF SEMA3C
AND NOTCHL IN HUMAN BRAIN
PERICYTES 25
2.2.1 GENERATION OF SEMA3C ISOFORMS 25
2.2.2 SEMA3C INHIBITS PROLIFERATION BUT HAS NO EFFECTS ON SURVIVAL OF
PERICYTES 28
2.2.3 SEMA3C AND NOTCH ACTIVATION HAVE NO EFFECT ON PERICYTE MIGRATION
29
2.2.4 SEMA3C ENHANCES PERICYTE ADHESION TO ENDOTHELIAL CELLS 31
2.3 FUNCTIONS
OF PERICYTE-DERIVED SEMA3C IN ANGIOGENESIS 31
2.3.1 SEMA3C INHIBITS ENDOTHELIAL CELL SPROUTING AND TUBE FORMATION 32
2.3.2 SEMA3C INHIBITS ENDOTHELIAL CELL MIGRATION 35
2.3.3 SEMA3C INHIBITS ENDOTHELIAL CELL SURVIVAL BUT HAS NO EFFECT ON
CELL PROLIFERATION..36
2.3.4 SEMA3C INHIBITS SPROUTING ANGIOGENESIS IN VIVO 36
2.4. DECIPHERING
THE MECHANISMS HOW SEMA3C INHIBITS
ANGIOGENESIS 41
2.4.1 TRANSCRIPTS THAT ARE REGULATED BY SEMA3C 41
2.4.2 SEMA3C REPELS ENDOTHELIAL CELLS AND CAUSES ENDOTHELIAL CELL
APOPTOSIS 41
2.4.3 SEMA3C DISRUPTS ENDOTHELIAL CELL JUNCTION INTEGRITY 44
2.4.4 SEMA3C INDUCES REORGANIZATION OF THE CYTOSKELETON AND CELL
DETACHMENT 45
2.4.5 SEMA3C INHIBITS FOCAL ADHESION KINASE SIGNALING 48
3. DISCUSSION 51
4. MATERIALS
AND METHODS 61
4.1 MATERIALS 61
4.1.1 OLIGONUCLEOTIDES 61
4.1.2 CELLS 62
I
HTTP://D-NB.INFO/1046729632
4.1.3 CELL CULTURE REAGENTS 62
4.1.4 GROWTH FACTORS, PROTEINS AND INHIBITORS 63
4.1.5 ANTIBODIES 64
4.1.6 KITS 64
4.1.7 SOLUTIONS AND BUFFERS 65
4.1.8 MISCELLANEOUS 66
4.2 METHODS 66
4.2.1 BIOCHEMICAL AND MOLECULAR BIOLOGY METHODS 66
4.2.1.1 DNA CLONING 66
4.2.1.2 GATEWAY CLONING 67
4.2.1.3 BACTERIAL CULTURE AND PLASMID PREPARATION 68
4.2.1.4 RNA ISOLATION 68
4.2.1.5 CDNA SYNTHESIS. 69
4.2.1.6 REAL-TIME PCR 69
4.2.1.7 MICROARRAY ANALYSIS 70
4.2.1.8 SDS-PAGE AND WESTERN BLOT 70
4.2.2 CELL CULTURE-BASED METHODS 71
4.2.2.1 CELL CULTURE CONDITIONS 71
4.2.2.2 ADENOVIRUS PRODUCTION 71
4.2.2.3 ADENOVIRUS TITTERING 72
4.2.2.4 LENTIVIRUS PRODUCTION 72
4.2.2.5 LENTIVIRUS TITTERING 73
4.2.2.6 CONDITIONED MEDIUM COLLECTION 73
4.2.3 IN VITRO ASSAYS 73
4.2.3.1 SPROUTING ASSAY 74
4.2.3.2 TUBE FORMATION ASSAY 74
4.2.3.3 PROLIFERATION ASSAY 75
4.2.3.4 CELL APOPTOSIS 75
4.2.3.5 TRANSWELL MIGRATION 76
4.2.3.6 WOUND HEALING ASSAY 76
4.2.3.7 CELL ADHESION 76
4.2.3.8 IMMUNOFLUORESCENT STAINING 77
4.2.3.9 TIME-LAPSE IMAGING 78
4.2.4 IN VIVO FUNCTIONAL ASSAYS 78
4.2.4.1 MATRIGEL PLUG ASSAY 78
4.2.4.2 MOUSE RETINAL ANGIOGENESIS 80
5. REFERENCES 81
ACKNOWLEDGMENTS 91
ABBREVIATIONS 93
UNITS 96
II
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| dewey-tens | 610 - Medicine and health 570 - Biology |
| discipline | Biologie Medizin |
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| language | English |
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| spelling | Yang, Wan-Jen Verfasser (DE-588)1045399027 aut The roles of semaphorin-3C in pericytes and endothelial cells presented by Yang, Wan-Jen [2013] VIII, 97 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Heidelberg, Univ., Diss., 2013 (DE-588)4113937-9 Hochschulschrift gnd-content DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=026922118&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Yang, Wan-Jen The roles of semaphorin-3C in pericytes and endothelial cells |
| subject_GND | (DE-588)4113937-9 |
| title | The roles of semaphorin-3C in pericytes and endothelial cells |
| title_auth | The roles of semaphorin-3C in pericytes and endothelial cells |
| title_exact_search | The roles of semaphorin-3C in pericytes and endothelial cells |
| title_full | The roles of semaphorin-3C in pericytes and endothelial cells presented by Yang, Wan-Jen |
| title_fullStr | The roles of semaphorin-3C in pericytes and endothelial cells presented by Yang, Wan-Jen |
| title_full_unstemmed | The roles of semaphorin-3C in pericytes and endothelial cells presented by Yang, Wan-Jen |
| title_short | The roles of semaphorin-3C in pericytes and endothelial cells |
| title_sort | the roles of semaphorin 3c in pericytes and endothelial cells |
| topic_facet | Hochschulschrift |
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