Retinal gene therapy: methods and protocols
This detailed volume describes a spectrum of methods and protocols that can be used for the bench-to-bedside development and evaluation of retinal gene therapy. Methods for the successful delivery of these gene therapy vector systems to the retina are examined, as well as assays to test the efficacy...
Gespeichert in:
| Weitere Verfasser: | , |
|---|---|
| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
New York, NY
Springer New York
2018
|
| Schriftenreihe: | Methods in Molecular Biology
1715 |
| Schlagworte: | |
| Online-Zugang: | UBR01 TUM01 URL des Erstveröffentlichers |
| Zusammenfassung: | This detailed volume describes a spectrum of methods and protocols that can be used for the bench-to-bedside development and evaluation of retinal gene therapy. Methods for the successful delivery of these gene therapy vector systems to the retina are examined, as well as assays to test the efficacy in vitro in cell cultures, for gene augmentation and gene editing in vivo on rodents, pigs, and monkey retinas, and on human retinal explants as well as in human clinical studies. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Retinal Gene Therapy: Methods and Protocols provides a wide range of readers from students to research experts with vital information on ocular gene therapy vector technology, in vitro and in vivo biological assays, and clinical protocols, to promote further studies for the benefit of children and adults with inherited retinal disease |
| Beschreibung: | 1 Online-Ressource (XVI, 380 Seiten) Illustrationen |
| ISBN: | 9781493975228 |
| DOI: | 10.1007/978-1-4939-7522-8 |
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| discipline | Biologie Medizin |
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| indexdate | 2024-07-10T08:05:33Z |
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| isbn | 9781493975228 |
| language | English |
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| physical | 1 Online-Ressource (XVI, 380 Seiten) Illustrationen |
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| spelling | Retinal gene therapy methods and protocols edited by Camiel J.F. Boon, Jan Wijnholds New York, NY Springer New York 2018 1 Online-Ressource (XVI, 380 Seiten) Illustrationen txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology 1715 This detailed volume describes a spectrum of methods and protocols that can be used for the bench-to-bedside development and evaluation of retinal gene therapy. Methods for the successful delivery of these gene therapy vector systems to the retina are examined, as well as assays to test the efficacy in vitro in cell cultures, for gene augmentation and gene editing in vivo on rodents, pigs, and monkey retinas, and on human retinal explants as well as in human clinical studies. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Retinal Gene Therapy: Methods and Protocols provides a wide range of readers from students to research experts with vital information on ocular gene therapy vector technology, in vitro and in vivo biological assays, and clinical protocols, to promote further studies for the benefit of children and adults with inherited retinal disease Biomedicine Human Genetics Medicine Human genetics Boon, Camiel J.F. 1980- (DE-588)1246480689 edt Wijnholds, Jan 1960- (DE-588)173614140 edt Erscheint auch als Druck-Ausgabe 978-1-4939-7521-1 https://doi.org/10.1007/978-1-4939-7522-8 Verlag URL des Erstveröffentlichers Volltext |
| spellingShingle | Retinal gene therapy methods and protocols Biomedicine Human Genetics Medicine Human genetics |
| title | Retinal gene therapy methods and protocols |
| title_auth | Retinal gene therapy methods and protocols |
| title_exact_search | Retinal gene therapy methods and protocols |
| title_full | Retinal gene therapy methods and protocols edited by Camiel J.F. Boon, Jan Wijnholds |
| title_fullStr | Retinal gene therapy methods and protocols edited by Camiel J.F. Boon, Jan Wijnholds |
| title_full_unstemmed | Retinal gene therapy methods and protocols edited by Camiel J.F. Boon, Jan Wijnholds |
| title_short | Retinal gene therapy |
| title_sort | retinal gene therapy methods and protocols |
| title_sub | methods and protocols |
| topic | Biomedicine Human Genetics Medicine Human genetics |
| topic_facet | Biomedicine Human Genetics Medicine Human genetics |
| url | https://doi.org/10.1007/978-1-4939-7522-8 |
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