Role of severe obesity in healing of muscle injuries:
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| 1. Verfasser: | |
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| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
Ulm
2018
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| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis Inhaltsverzeichnis |
| Beschreibung: | ix, 123, XXV Blätter Illustrationen, Diagramme 30 cm |
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| 100 | 1 | |a Werner, Jens-Uwe |e Verfasser |0 (DE-588)1162848456 |4 aut | |
| 245 | 1 | 0 | |a Role of severe obesity in healing of muscle injuries |c submitted by Jens-Uwe Werner |
| 264 | 1 | |a Ulm |c 2018 | |
| 300 | |a ix, 123, XXV Blätter |b Illustrationen, Diagramme |c 30 cm | ||
| 336 | |b txt |2 rdacontent | ||
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| 338 | |b nc |2 rdacarrier | ||
| 502 | |b Dissertation |c Universität Ulm |d 2018 | ||
| 653 | |a Obesity. | ||
| 653 | |a Wounds and injuries. | ||
| 655 | 7 | |0 (DE-588)4113937-9 |a Hochschulschrift |2 gnd-content | |
| 710 | 2 | |a Universität Ulm |0 (DE-588)30607-1 |4 dgg | |
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Datensatz im Suchindex
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|---|---|
| adam_text | TABLE OF CONTENTS
1
2
INTRODUCTION.............................................................................................................
1.1 OBESITY YESTERDAY, TODAY AND ITS CONSEQUENCES
................................................
1.2 FREE FATTY ACIDS, CHRONIC INFLAMMATION, AND CO-MORBIDITIES IN
OBESITY
.............
1.3 STRUCTURE AND FUNCTION OF SKELETAL
MUSCLE.........................................................
1.4 MACROPHAGES AND SATELLITE
CELLS........................................................................
1.5 THE PRINCIPLE BEHIND MUSCLE
INJURY..................................................................
1.6 FIBROSIS AND THE COMPLEMENT SYSTEM IN MUSCLE REGENERATION
...........................
1.7 MUSCLE REGENERATION IN DETAIL, UNDERLYING MECHANISMS AND THE ROLE OF
OBESITY
1.8 AIM OF THE
STUDY...............................................................................................
MATERIAL AND
METHODS..............................................................................................
2.1
MATERIALS...........................................................................................................
2.1.1
CONSUMABLES..............................................................................................
2.1.2 SOLUTIONS AND REAGENTS
...............................................................................
2.1.3
DEVICES......................................................................................................
2.1.4 ANESTHESIA, ANIMALS, AND CHOW
...................................................................
2.1.5 K
ITS............................................................................................................
2.1.6 PRIMER FOR
QPCR.........................................................................................
2.1.7
SOFTWARE.....................................................................................................
.
2.1.8 MICROARRAY
TOOLS.........................................................................................
.
2.2
METHODS............................................................................................................
2.2.1 ANIMAL HOUSING AND
BREEDING.....................................................................
2.2.2 TRAUMATIC MUSCLE
INJURY.............................................................................
2.2.3 HEMATOXYLIN AND EOSIN (HE)
STAINING........................................................
2.2.4 SIRIUS RED STAINING
.....................................................................................
2.2.5 IMMUNOHISTOCHEMICAL
STAINING...................................................................
2.2.6 IN VIVO IMAGING
..........................................................................................
2.2.7 LIPID EXTRACTION AND GAS CHROMATOGRAPHY
..................................
,
..............
...1
...1
...1
...3
...5
...7
..8
.9
14
16
16
16
17
19
20
20
21
21
22
23
23
23
26
26
26
27
28
2.2.8 RNA ISOLATION AND MICROARRAY
ANALYSIS.........................................................29
2.2.9 PATHWAY
ANALYSIS...........................................................................................
31
2.2.10 REAL-TIME
QPCR.............................................................................................
31
2.2.11 STATISTICAL
EVALUATION........................................................................................32
RESULTS.........................................................................................................................33
3.1 ESTABLISHING A MODEL FOR NORMAL WEIGHT AND OBESE C57BL/6J MICE
...................
33
3.1.1 HIGH-FAT DIET INFLUENCES OUR TRAUMA MODEL IN AN EXPECTED WAY
....................
33
3.1.2 INFLUENCE OF DIET-INDUCED OBESITY ON THE INJURY MODEL
..................................
34
3.1.3 INFLUENCE OF INJURY ON THE DIET-INDUCED OBESITY MODEL
..................................
36
3.2 INJURY TO EXTENSOR ILIOTIBIALIS ANTICUS LEADS TO TISSUE DISRUPTION
WITH SUBSEQUENT
REGENERATION.....................................................................................................................37
3.2.1 VALIDATING THE PROGRESSION OF INJURY BY MRI, AND HE-STAINING
.....................
37
3.3 FIBROSIS FORMATION IS ALTERED IN THE OBESE PHENOTYPE
..........................................
40
3.3.1 GENE EXPRESSION LEVELS IN FIBROSIS ARE DRAMATICALLY LOWERED IN
OBESITY
........
41
3.3.2 PATHWAY ANALYSIS OF FIBROSIS-RELATED
GENES....................................................44
3.4 FATTY ACID PROFILES IN MOUSE SKELETAL
MUSCLE........................................................45
3.4.1 COMPARING FATTY ACID RATIOS IN NORMAL WEIGHT AND OBESE ANIMALS
..................
46
3.4.2 PHOSPHOLIPID FRACTION
ANALYSIS.......................................................................48
3.4.3 TRIGLYCERIDE FRACTION
ANALYSIS........................................................................49
3.4.4 LIPID METABOLISM-RELATED GENE EXPRESSION IS SEVERELY ALTERED IN
OBESITY
....
51
3.4.5 PATHWAY ANALYSIS OF LIPID
METABOLISM........................................................... 54
3.5 MOLECULAR FINGERPRINT OF INJURED NORMAL WEIGHT AND OBESE C57BL/6J
MICE
.......
54
3.5.1 UNBIASED MULTI-STEP PROGRAM TO ASSESS DIFFERENTIALLY EXPRESSED
GENES
.........
55
3.5.2 REDUCTION IN QUANTITATIVE GENE RESPONSE TO INJURY IN OBESE MICE
..................
60
3.5.3 MINIMAL OVERLAP OF DIFFERENTIALLY EXPRESSED GENES AFTER MUSCLE
INJURY
........
60
3.5.4 SCREENING FOR HIGH IMPACT GENES IN MUSCLE REGENERATION
..............................
61
3.5.5 PATHWAY ANALYSIS OF HIGH IMPACT GENES IN MUSCLE REGENERATION
....................
64
3.6 OBESITY AND ITS INFLUENCE ON MUSCLE REGENERATION
67
3.6.1 CELLULAR KEY PLAYERS REQUIRED FOR MUSCLE REGENERATION,
3.6.2 COMPLEMENT SYSTEM IN MUSCLE REGENERATION
................................................
71
3.6.3 VALIDATION OF GENES IN HISTOLOGY AND QPCR
..................................................
72
3.6.4 ENERGY HOMEOSTASIS IS INFLUENCED BY BLUNT MUSCLE INJURY
...........................
75
3.6.5 CELL MAINTENANCE IS REQUIRED FOR PROPER REGENERATION
....................................
76
4
DISCUSSION....................................................................................................................
82
4.1 BLUNT MUSCLE INJURY IN NORMAL WEIGHT AND OBESE C57BL/6J MICE
........................
82
4.2 MUSCLE TRAUMA AND ITS IMPLICATIONS ON MORPHOLOGY
.............................................
83
4.3 FIBROUS TISSUE IS AFFECTED BY OBESITY
....................................................................
84
4.4 OBESITY INFLUENCES FATTY ACID CONTENT IN SKELETAL MUSCLE
......................................
88
4.5 THE MOLECULAR FINGERPRINT IN RESPONSE TO INJURY SUBSTANTIALLY
DIFFERS IN OBESITY ..91
5
SUMMARY....................................................................................................................
101
6
REFERENCES.................................................................................................................
102
SUPPLEMENTARY
INFORMATION................................................................................................
I
STATUTORY DECLARATION
|
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| author | Werner, Jens-Uwe |
| author_GND | (DE-588)1162848456 |
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| bvnumber | BV045376962 |
| ctrlnum | (OCoLC)1136398075 (DE-599)BSZ507663284 |
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| spelling | Werner, Jens-Uwe Verfasser (DE-588)1162848456 aut Role of severe obesity in healing of muscle injuries submitted by Jens-Uwe Werner Ulm 2018 ix, 123, XXV Blätter Illustrationen, Diagramme 30 cm txt rdacontent n rdamedia nc rdacarrier Dissertation Universität Ulm 2018 Obesity. Wounds and injuries. (DE-588)4113937-9 Hochschulschrift gnd-content Universität Ulm (DE-588)30607-1 dgg B:DE-101 application/pdf http://d-nb.info/1166352935/04 Inhaltsverzeichnis DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=030763358&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Werner, Jens-Uwe Role of severe obesity in healing of muscle injuries |
| subject_GND | (DE-588)4113937-9 |
| title | Role of severe obesity in healing of muscle injuries |
| title_auth | Role of severe obesity in healing of muscle injuries |
| title_exact_search | Role of severe obesity in healing of muscle injuries |
| title_full | Role of severe obesity in healing of muscle injuries submitted by Jens-Uwe Werner |
| title_fullStr | Role of severe obesity in healing of muscle injuries submitted by Jens-Uwe Werner |
| title_full_unstemmed | Role of severe obesity in healing of muscle injuries submitted by Jens-Uwe Werner |
| title_short | Role of severe obesity in healing of muscle injuries |
| title_sort | role of severe obesity in healing of muscle injuries |
| topic_facet | Hochschulschrift |
| url | http://d-nb.info/1166352935/04 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=030763358&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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