Antibody array for the detection of protein phosphorylation, localization and application to signaling pathways in human cancer cell lines:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
2004
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | 134 S. graph. Darst. 30 cm |
Internformat
MARC
| LEADER | 00000nam a2200000 c 4500 | ||
|---|---|---|---|
| 001 | BV021288876 | ||
| 003 | DE-604 | ||
| 007 | t | ||
| 008 | 060112s2004 gw d||| m||| 00||| eng d | ||
| 015 | |a 05,H02,1288 |2 dnb | ||
| 016 | 7 | |a 972777717 |2 DE-101 | |
| 035 | |a (OCoLC)634594169 | ||
| 035 | |a (DE-599)BVBBV021288876 | ||
| 040 | |a DE-604 |b ger |e rakddb | ||
| 041 | 0 | |a eng | |
| 044 | |a gw |c XA-DE | ||
| 049 | |a DE-355 | ||
| 084 | |a 570 |2 sdnb | ||
| 100 | 1 | |a Wang, Lin |d 1964- |e Verfasser |0 (DE-588)129521094 |4 aut | |
| 245 | 1 | 0 | |a Antibody array for the detection of protein phosphorylation, localization and application to signaling pathways in human cancer cell lines |c von Lin Wang |
| 264 | 1 | |c 2004 | |
| 300 | |a 134 S. |b graph. Darst. |c 30 cm | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 502 | |a Jena, Univ., Diss., 2004. - Zsfassung in engl. und dt. Sprache | ||
| 650 | 0 | 7 | |a Proteine |0 (DE-588)4076388-2 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Microarray |0 (DE-588)4544227-7 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Antikörper |0 (DE-588)4002290-0 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Phosphorylierung |0 (DE-588)4174459-7 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Signaltransduktion |0 (DE-588)4318717-1 |2 gnd |9 rswk-swf |
| 655 | 7 | |0 (DE-588)4113937-9 |a Hochschulschrift |2 gnd-content | |
| 689 | 0 | 0 | |a Signaltransduktion |0 (DE-588)4318717-1 |D s |
| 689 | 0 | 1 | |a Proteine |0 (DE-588)4076388-2 |D s |
| 689 | 0 | 2 | |a Phosphorylierung |0 (DE-588)4174459-7 |D s |
| 689 | 0 | 3 | |a Microarray |0 (DE-588)4544227-7 |D s |
| 689 | 0 | 4 | |a Antikörper |0 (DE-588)4002290-0 |D s |
| 689 | 0 | |5 DE-604 | |
| 856 | 4 | 2 | |m DNB Datenaustausch |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=014609765&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
| 943 | 1 | |a oai:aleph.bib-bvb.de:BVB01-014609765 | |
Datensatz im Suchindex
| _version_ | 1807503884440043520 |
|---|---|
| adam_text |
TABLES
OF
CONTENTS
1
INTRODUCTION.
1
1.1
ANTIBODY
ARRAY
TECHNOLOGIES
.
1
1.2
PROTEIN
PHOSPHORYLATION,
LOCALIZATION
AND
FUNCTION.
2
1.3
INTERACTION
PROTEOMICS
AND
PATHWAY
BUILDING.
3
1.3.1
BMP2
SIGNALLING
.
4
1.3.2
THE
EFFECT
OF
STI
571
ON
CELL
SIGNALLING
.
9
1.4
AIM
.
10
2
MATERIALS
AND
METHODS.
11
2.1
MATERIALS.
11
2.1.1
CELL
LINES
.
11
2.1.2
CHEMICALS
.
11
2.1.3
ANTIBODIES
.
11
2.1.4
PROTEIN-MOLECULAR
WEIGHT
MARKER
.
11
2.1.5
APPARATUS
.
11
2.2
METHODS.
12
2.2.1
CELL
CULTURE
.
12
2.2.2
CELL
NUMBERS
AND
EXPERIMENTAL
FORMAT.
12
2.2.3
CYTOPLASMIC
AND
NUCLEAR
PROTEIN
EXTRACTS
.
12
2.2.4
DETERMINATION
OF
PROTEIN
CONCENTRATION
.
13
2.2.5
PONCEAU
S
STAINING
.
13
2.2.6
WESTERN
ANALYSIS
.
14
2.2.7
IMMUNOPRECIPITATION.
14
2.2.8
CY3
AND
CY5
STAINING
IN
SDS-PAGE
.
14
2.2.9
CY3AND
CY5
STAINING
OF
MICROSCOPE-SLIDE-SIZE
ARRAY
.
14
2.2.10
CY3
AND
CY5
STAINING
OF
MINI-ARRAYSLIDE
.
15
2.2.11
SILVER
STAINING
OF
MINI-ARRAYSLIDE
.
15
2.2.12
SILVER
STAINING
IN
ARRAYTUBE
.
15
2.2.12.1
BIOTINYLATION
OF
CELLULAR
PROTEINS
.
15
2.2.12.2
INCUBATION
OF
PROTEIN
EXTRACTS
.
15
2.2.12.3
SILVER
STAINING
.
16
2.2.13
STATISTICAL
ANALYSIS
.
16
3
RESULTS
.
17
3.1
ANTIBODY
ARRAY
TECHNOLOGIES
.
17
3.1.1CY3
AND
CY5
OF
SDS-PAGE,
MICROSCOPE-SLIDE-SIZE
ARRAY,
MINI-ARRAYSLIDE
.
17
3.1.2
DATA
ANALYSIS
.
S
.
20
3.2
MODULATION
OF
PHOSPHORYLATION
AND
LOCALIZATION
IN
BMP2
TREATED
U937
CELLS.
26
3.2.1
TOTAL
P-TYROSINE
AND
P-P38
INCREASED:
CYTOPLASM
AND
NUCLEUS
INCREASING.
29
3.2.2
TOTAL
P-ERK
AND
P-JNK
INCREASED:
CYTOPLASM
INCREASING
AND
NUCLEUS
UNCHANGED
.
32
3.2.3
TOTAL
P-AKT
INCREASED:
CYTOPLASM
INCREASING
AND
NUCLEUS
DECREASING
.
34
3.2.4
TOTAL
P-SMAD1,
P-SMAD2/3
AND
C
MYC
UNCHANGED:
CYTOPLASM
DECREASING
AND
NUCLEUS
INCREASING.
35
3.2.5
TOTAL
P-P70S6
UNCHANGED:
CYTOPLASM
INCREASING
AND
NUCLEUS
DECREASING
39
3.3
MODULATION
OF
PHOSPHORYLATION
AND
LOCALIZATION
IN
BMP2
TREATED
MCF7
CELLS
.
40
3.3.1
TOTAL
P-JNK,
P-SMAD1,
P-SMAD2/3,
P-P38
AND
P38
UNCHANGED:
CYTOPLASM
INCREASING
AND
NUCLEUS
DECREASING
.
3.3.2
P-TYROSINE,
P-STAT3,
P-ERK
AND
P-P70S6
INCREASED:
CYTOPLASM
INCREASING
AND
NUCLEUS
UNCHANGED
.
43
3.3.3TOTAL
C-MYC
UNCHANGED:
NUCLEUS
SLIGHTLY
INCREASING
AND
CYTOPLASM
SLIGHTLY
DECREASING
.
46
3.3.4
TOTAL
P-AKT
UNCHANGED:
NUCLEUS
AND
CYTOPLASM
UNCHANGED.
47
3.4
MODULATION
OF
PHOSPHORYLATION
AND
LOCALIZATION
IN
STI
TREATED
K562
CELLS
.
47
3.4.1
TOTAL
P-AKT
AND
P-P70S6
DECREASED:
CYTOPLASM
AND
NUCLEUS
DECREASING.
47
3.4.2
TOTAL
P-P38,
P38,
C-MYC,
P-TYROSINE,
P-SMAD1
AND
P-SMAD2/3
INCREASED:
CYTOPLASM
AND
NUCLEUS
INCREASING.
48
3.4.3TOTAL
P-STAT3
AND
P-JNK
INCREASED:
CYTOPLASM
INCREASING
AND
NUCLEUS
UNCHANGED
.
48
3.4.4
TOTAL
P-ERK
INCREASED:
CYTOPLASM
DECREASING
AND
NUCLEUS
INCREASING.
48
4
DISCUSSION
.
52
4.1
ANTIBODY
ARRAY
TECHNOLOGIES
.
52
4.2
BMP2
SIGNALLING
IN
U937
CELLS
.
54
4.2.1
BMP2
ACTIVATES
AKT,
ERK
AND
JNK
PATHWAY
BY
INCREASE
OF
CYTOPLASMIC
PHOSPHORYLATION
IN
U937
CELLS
.
54
4.2.2
BMP2
ACTIVATES
SMADL
AND
SMAD2/3
NETWORK,
P38
NETWORK
AND
C-MYC,
TYROSINE
NETWORK
BY
INCREASE
OF
NUCLEAR
PHOSPHORYLATION
IN
U937
CELLS
.
55
4.2.3
BMP2
INHIBITS
P70S6
SIGNALLING
BY
INCREASE
OF
CYTOPLASMIC
PHOSPHORYLATION
AND
DECREASE
OF
NUCLEUS
IN
U937
CELLS
.
57
4.2.4POSSIBLE
INTRACELLULAR
RELATIONSHIP
IN
BMP2
TREATED
U937
CELLS
.
57
4.3
BMP2
SIGNALLING
IN
MCF7
CELLS
.
58
4.3.1
BMP2
BMP2
ACTIVATES
P70S6
AND
ERK
SIGNALING,
TYROSINE
AND
STAT3
NETWORK
BY
INCREASE
OF
CYTOPLASMIC
PHOSPHORYLATION
IN
MCF-7
CELLS
.
58
4.3.2
BMP2
INHIBITS
P38,
JNK
AND
SMAD
SIGNALLING
BY
INCREASE
OF
CYTOPLASMIC
PHOSPHORYLATION
AND
DECREASE
OF
NUCLEUS
IN
MCF-7
CELLS
.
59
4.3.3
BMP2
ACTIVATES
C-MYC
SIGNALLING
BY
INCREASE
OF
NUCLEAR
PHOSPHORYLATION
IN
MCF-7
CELLS.
.60
4.3.4POSSIBLE
INTRACELLULAR
RELATIONSHIP
IN
BMP2
TREATED
MCF7
CELLS
.
60
4.4
STI
SIGNALLING
IN
K562
CELLS
.
60
4.4.1
STI571
INHIBITS
AKT/P70S6
PI3K
SIGNALLING
BY
DECREASE
OF
CYTOPLASMIC
AND
NUCLEAR
PHOSPHORYLATION
IN
K562
CELLS
.
61
4.4.2
STI571
ACTIVATES
P38
MAPK
PATHWAY,
C-MYC,
TYROSINE
AND
SMAD1/SMAD2/3
NETWORKS
BY
INCREASE
OF
CYTOPLASMIC
AND
NUCLEAR
PHOSPHORYLATION
IN
K562
CELLS
.
61
4.4.3
STI571
ACTIVATES
PATHWAY
BY
INCREASE
OF
NUCLEAR
PHOSPHORYLATION
AND
DECREASE
OF
CYTOPLASM
IN
K562
CELLS
.
61
4.4.4
STI571
ACTIVATES
STAT3
NETWORK
AND
JNK/MAPK
PATHWAY
BY
INCREASE
OF
CYTOPLASMIC
PHOSPHORYLATION
IN
K562
CELLS
.
62
4.4.5POSSIBLE
INTRACELLULAR
RELATIONSHIP
IN
STI
TREATED
K562
CELLS
.
62
4.5
MODELS
OF
SIGNALLING
PROTEIN
AND
CELLULAR
RESPONSE
.
63
5
SUMMARY.
68
6
ZUSAMMENFASSUNG.
70
7
REFERENCES.
72 |
| adam_txt | |
| any_adam_object | 1 |
| any_adam_object_boolean | |
| author | Wang, Lin 1964- |
| author_GND | (DE-588)129521094 |
| author_facet | Wang, Lin 1964- |
| author_role | aut |
| author_sort | Wang, Lin 1964- |
| author_variant | l w lw |
| building | Verbundindex |
| bvnumber | BV021288876 |
| ctrlnum | (OCoLC)634594169 (DE-599)BVBBV021288876 |
| discipline | Biologie |
| discipline_str_mv | Biologie |
| format | Thesis Book |
| fullrecord | <?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>00000nam a2200000 c 4500</leader><controlfield tag="001">BV021288876</controlfield><controlfield tag="003">DE-604</controlfield><controlfield tag="007">t</controlfield><controlfield tag="008">060112s2004 gw d||| m||| 00||| eng d</controlfield><datafield tag="015" ind1=" " ind2=" "><subfield code="a">05,H02,1288</subfield><subfield code="2">dnb</subfield></datafield><datafield tag="016" ind1="7" ind2=" "><subfield code="a">972777717</subfield><subfield code="2">DE-101</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(OCoLC)634594169</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)BVBBV021288876</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-604</subfield><subfield code="b">ger</subfield><subfield code="e">rakddb</subfield></datafield><datafield tag="041" ind1="0" ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="044" ind1=" " ind2=" "><subfield code="a">gw</subfield><subfield code="c">XA-DE</subfield></datafield><datafield tag="049" ind1=" " ind2=" "><subfield code="a">DE-355</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">570</subfield><subfield code="2">sdnb</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Wang, Lin</subfield><subfield code="d">1964-</subfield><subfield code="e">Verfasser</subfield><subfield code="0">(DE-588)129521094</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Antibody array for the detection of protein phosphorylation, localization and application to signaling pathways in human cancer cell lines</subfield><subfield code="c">von Lin Wang</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2004</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">134 S.</subfield><subfield code="b">graph. Darst.</subfield><subfield code="c">30 cm</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="b">n</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="b">nc</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="502" ind1=" " ind2=" "><subfield code="a">Jena, Univ., Diss., 2004. - Zsfassung in engl. und dt. Sprache</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Proteine</subfield><subfield code="0">(DE-588)4076388-2</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Microarray</subfield><subfield code="0">(DE-588)4544227-7</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Antikörper</subfield><subfield code="0">(DE-588)4002290-0</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Phosphorylierung</subfield><subfield code="0">(DE-588)4174459-7</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Signaltransduktion</subfield><subfield code="0">(DE-588)4318717-1</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="655" ind1=" " ind2="7"><subfield code="0">(DE-588)4113937-9</subfield><subfield code="a">Hochschulschrift</subfield><subfield code="2">gnd-content</subfield></datafield><datafield tag="689" ind1="0" ind2="0"><subfield code="a">Signaltransduktion</subfield><subfield code="0">(DE-588)4318717-1</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2="1"><subfield code="a">Proteine</subfield><subfield code="0">(DE-588)4076388-2</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2="2"><subfield code="a">Phosphorylierung</subfield><subfield code="0">(DE-588)4174459-7</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2="3"><subfield code="a">Microarray</subfield><subfield code="0">(DE-588)4544227-7</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2="4"><subfield code="a">Antikörper</subfield><subfield code="0">(DE-588)4002290-0</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2=" "><subfield code="5">DE-604</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="m">DNB Datenaustausch</subfield><subfield code="q">application/pdf</subfield><subfield code="u">http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=014609765&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA</subfield><subfield code="3">Inhaltsverzeichnis</subfield></datafield><datafield tag="943" ind1="1" ind2=" "><subfield code="a">oai:aleph.bib-bvb.de:BVB01-014609765</subfield></datafield></record></collection> |
| genre | (DE-588)4113937-9 Hochschulschrift gnd-content |
| genre_facet | Hochschulschrift |
| id | DE-604.BV021288876 |
| illustrated | Illustrated |
| index_date | 2024-07-02T13:49:19Z |
| indexdate | 2024-08-16T01:00:29Z |
| institution | BVB |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-014609765 |
| oclc_num | 634594169 |
| open_access_boolean | |
| owner | DE-355 DE-BY-UBR |
| owner_facet | DE-355 DE-BY-UBR |
| physical | 134 S. graph. Darst. 30 cm |
| publishDate | 2004 |
| publishDateSearch | 2004 |
| publishDateSort | 2004 |
| record_format | marc |
| spelling | Wang, Lin 1964- Verfasser (DE-588)129521094 aut Antibody array for the detection of protein phosphorylation, localization and application to signaling pathways in human cancer cell lines von Lin Wang 2004 134 S. graph. Darst. 30 cm txt rdacontent n rdamedia nc rdacarrier Jena, Univ., Diss., 2004. - Zsfassung in engl. und dt. Sprache Proteine (DE-588)4076388-2 gnd rswk-swf Microarray (DE-588)4544227-7 gnd rswk-swf Antikörper (DE-588)4002290-0 gnd rswk-swf Phosphorylierung (DE-588)4174459-7 gnd rswk-swf Signaltransduktion (DE-588)4318717-1 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Signaltransduktion (DE-588)4318717-1 s Proteine (DE-588)4076388-2 s Phosphorylierung (DE-588)4174459-7 s Microarray (DE-588)4544227-7 s Antikörper (DE-588)4002290-0 s DE-604 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=014609765&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Wang, Lin 1964- Antibody array for the detection of protein phosphorylation, localization and application to signaling pathways in human cancer cell lines Proteine (DE-588)4076388-2 gnd Microarray (DE-588)4544227-7 gnd Antikörper (DE-588)4002290-0 gnd Phosphorylierung (DE-588)4174459-7 gnd Signaltransduktion (DE-588)4318717-1 gnd |
| subject_GND | (DE-588)4076388-2 (DE-588)4544227-7 (DE-588)4002290-0 (DE-588)4174459-7 (DE-588)4318717-1 (DE-588)4113937-9 |
| title | Antibody array for the detection of protein phosphorylation, localization and application to signaling pathways in human cancer cell lines |
| title_auth | Antibody array for the detection of protein phosphorylation, localization and application to signaling pathways in human cancer cell lines |
| title_exact_search | Antibody array for the detection of protein phosphorylation, localization and application to signaling pathways in human cancer cell lines |
| title_exact_search_txtP | Antibody array for the detection of protein phosphorylation, localization and application to signaling pathways in human cancer cell lines |
| title_full | Antibody array for the detection of protein phosphorylation, localization and application to signaling pathways in human cancer cell lines von Lin Wang |
| title_fullStr | Antibody array for the detection of protein phosphorylation, localization and application to signaling pathways in human cancer cell lines von Lin Wang |
| title_full_unstemmed | Antibody array for the detection of protein phosphorylation, localization and application to signaling pathways in human cancer cell lines von Lin Wang |
| title_short | Antibody array for the detection of protein phosphorylation, localization and application to signaling pathways in human cancer cell lines |
| title_sort | antibody array for the detection of protein phosphorylation localization and application to signaling pathways in human cancer cell lines |
| topic | Proteine (DE-588)4076388-2 gnd Microarray (DE-588)4544227-7 gnd Antikörper (DE-588)4002290-0 gnd Phosphorylierung (DE-588)4174459-7 gnd Signaltransduktion (DE-588)4318717-1 gnd |
| topic_facet | Proteine Microarray Antikörper Phosphorylierung Signaltransduktion Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=014609765&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT wanglin antibodyarrayforthedetectionofproteinphosphorylationlocalizationandapplicationtosignalingpathwaysinhumancancercelllines |