Multiphotonen-Mikroskopie und Laser-Nanochirurgie der Kornea mittels Naher-Infrarot Nanojoule Femtosekunden Laserpulse: = Animal studies on Cornea nanosurgery and multiphoton microscopy with nanojoule femtosecond near-infrared lasers
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| Format: | Buch |
| Sprache: | English |
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2006
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| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | Zsfassung in dt. Sprache. - Jena, Univ., Diss., 2006 |
| Beschreibung: | III, 101 Bl. Ill. |
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| 245 | 1 | 0 | |a Multiphotonen-Mikroskopie und Laser-Nanochirurgie der Kornea mittels Naher-Infrarot Nanojoule Femtosekunden Laserpulse |b = Animal studies on Cornea nanosurgery and multiphoton microscopy with nanojoule femtosecond near-infrared lasers |c Bao-Gui Wang |
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Datensatz im Suchindex
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| adam_text | Wang BG
Table of contents
Summary 1
1. Introduction 5
1. History and advances in laser refractive surgery 5
1.1 Radial keratotomy (RK) 5
1.2 Excimer laser-assisted photorefractive keratectomy (PRK) 6
1.3 Laser epithelial keratomileusis (LASEK) and Epi-LASIK 7
1.4 Laser-assisted in-situ keratomileusis (LASIK) 9
1.5Femto-LASIK(IntraLase) 12
1.6 New approaches being just under investigations 13
2. Histology of cornea (New Zealand Albino rabbits) 14
2.1 Description of cornea histology 15
2.1.1 Epithelium 15
2.1.2 Bowman s layer 16
2.1.3 Stroma 17
2.1.4 Descemet s layer 19
2.1.5 Endothelium 20
2.2 Corneal innervations 21
2.3 Factors maintaining corneal transparency 22
2.3.1 Collagen fibrils and lamellas 22
2.3.2 Stromal hydration 23
2.3.3 Function of endothelium 23
2.3.4 Other relevant factors 24
3. Mechanism and strategy of nanoprocessing in bulk tissue 24
4. Application of femtosecond lasers in biomedical fields 28
4.1 Diagnostics: Multiphoton microscopy 28
4.1.1 Two-photon autofluorescence (2PF) 29
4.1.2 Second harmonic generation (SHG) 30
4.2 Corneal Surgery 31
4.2.1 Intrastromal ablation with epithelial intact 31
4.2.2 Multiphoton-mediated generation of corneal flap and intrastromal
lenticule 32
2. Purposes of this work 34
3. Materials and Methods 35
1. Multiphoton microscope 35
2. Laser systems 36
2.1 Chameleon 36
2.2MaiTai 37
2.3 Coherent Vitesse 38
3. Threshold value for intrastromal nanosurgery and optimized parameters
for multiphoton microscopy 39
4. Animals, narcosis, and disinfection 40
5. Tissue-processing postoperation: specimen preparation, harvest and
analysis 41
4. Results 44
II
Wang BG
1. In-vivo corneal optical nonlinear imaging based on multiphoton
microscopy 44
1.1 Optical tomography of corneal tissue based on 2PF 44
1.1.1 Three-dimensional imaging of epithelial cells (three sublayers) 46
1.1.2 Three-dimensional imaging of stromal keratocytes 47
1.2 Optical tomography of stromal collagen lamellas based on SHG 47
1.3 Comparison of optical nonlinear images based on 2PF and SHG 48
2. In-vivo application of multiphoton imaging in viewing intrastromal
surgery 49
2.1 Evaluating intrastromal nanosurgery 50
2.2 Advantages of SHG in visualizing intrastromal surgery 50
2.2.1 Physiological architecture of collagen lamellas imaged with SHG 50
2.2.2 Observations of intrastromal surgery 24 hours postoperation 51
3. In-vivo intrastromal ablation with epithelium intact 53
3.1 Optical imaging during laser intrastromal ablation 53
3.2 Histological observations on intrastromal ablation (immediate results) 54
3.3 Follow-up on wound repair of intrastromal ablation 55
3.4 Cell activations in the treated cornea during wound healing process 56
4. In-vivo multiphoton-mediated generation of corneal flap 57
4.1 Optical observations during performing of corneal flaps 57
4.2 Immediate outcomes of corneal flaps 57
4.3 Follow-up on wound repair of the unlifted corneal flaps 58
5. Simultaneous generation of corneal flap and stromal lenticule 64
6. Observations on cells activation and migration in cornea during wound
repair 66
6.1 Optical tomography of activated keratocytes (myofibroblasts) 66
6.2 Histological observations: migrating inflammatory cells and discrimination
of eosinophils 67
5. Discussion, conclusions and outlook 70
1. Multiphoton tomography and nanoprocessing 70
1.1 Application of multiphoton microscopy in visualizing intrastromal surgery 70
1.2 Intrastromal ablation with epithelium intact 74
1.3 Multiphoton-mediated corneal flap generation 75
2. Wound repair after nanojoule ultrashort laser surgery in cornea 77
6. References 79
7. Appendix 91
8. List of figures and diagrams 93
9. Explanation (Selbstandigkeitserklarung) 95
10. Curriculum Vitae 96
11. Published and submitted works, Awards and advanced courses 97
12. Acknowledgments 100
III
Wang BG
List of Figures and Diagrams
Fig.1 Diagrams illustrating radial keratotomy (RK) P6
Fig.2 Diagrams illustrating PRK P6
Fig.3 Procedures of LASEK P8
Fig.4 Procedures of Epi-LASIK P9
Fig.4_1 Histological sections of Epi-LASIK P9
Fig.5 Procedures of LASIK P10
Fig.5_1 Photograph of microkeratome P10
Fig.5_2 Flap-related complications P11
Fig.6 Procedures of Femto-LASIK P12
Fig.6_1 Smooth flap bed generated with femtosecond high pulse energy lasers P13
Fig.7 All-Femto-LASIK P14
Fig.8_1 Imitated diagram of histological cross-section of rabbit cornea P16
Fig.8_2 Three-dimensional illustration of corneal components P16
Fig.8_3 TEM of corneal stroma P18
Fig.9 Diagram depicting noncentrosymmetric collagen structure P19
Fig.10 Nerve fibres in cornea displayed by gold chloride and optical imaging P22
Fig.11 Schematic of intrastromal surgery induced by nanojoule ultrashort laser pulses P25
Fig.12 Photographic illustration of animal laser eye surgery P35
Fig.12_1 Modified photograph of laser operations on rabbit P37
Fig.12_2 Schematic of experimental setup for multiphoton microscopy and intrastromal surgery.... P38
Fig.12_3 Schematic illustrating intrastromal ablation P38
Fig.13 Threshold power for intratissue surgery and optimized power for multiphoton imaging P39
Fig.14 An indispensable instrument in this work: Zeissslit lamp 120 P42
Fig.14_1 Epithelial marks taken by slit lamp P42
Fig.15 Comparison of average central cornea thickness of rabbits, human and porcine P44
Fig.16_1 Nonlinear optical images of epithelial squamous cells and basal cells P45
Fig.16_2 Nonlinear optical images of keratocytes and endothelial cells P45
Fig.17_1 Optical nonlinear tomography of epithelium P46
Fig. 17_2 Optical nonlinear tomography of stromal keratocytes P47
Fig.17_3 In-vivo collagen lamellar nonlinear optical sectioning P48
Fig.18 Nonlinear optical imaging of stromal tissue with different wavelengths P49
Fig.19 Real-time nonlinear optical observations of intrastromal femtosecond laser nanosurgery P50
Fig.20 SHG image and A-scanning of emission wavelength P51
Fig.20_1 SHG observations on intrastromal nanosurgery 24 hours postoperation P51
Fig.20_2 Reflexion imaging of intrastromal nanosurgery 24 hours postoperation P52
Fig.21_1 On-line optical observations on intrastromal ablation P53
Fig.21_2 Histological cross-sections displaying the immediate ablation outcomes P54
-93-
Wang BG
Fig.21_3 Histological observations on wound repair of intrastromal ablation at 4th and 7th days P55
Fig.21_4 Optical detecting on emergence of myofibroblasts P56
Fig.22_1 Optical images during flap generation P57
Fig.22_2 Photographs of corneal flaps taken immediately after the laser operation P58
Fig.23SEM of the lifted corneal flap P58
Fig.24 Observations on wound repair at 1st day P59
Fig.24_1 Histological observations on the migrating inflammatory cells at 1st day P59
Fig.25_1 Histological observations of aflap at 7th day postoperation P60
Fig.25_2 Flap at 16th day P61
Fig.25_3 Flap at 28th day P62
Fig.25_4 Flap at 66th day P63
Fig.25_5 Flap at 90th day P64
Fig.26 Photograph showing simultaneous generation of corneal flap and intrastromal lenticule P65
Fig.27 In-vivo cellular nonlinear optical tomography of myofibroblasts based on 2PF P66
Fig.27_1 In-vivo optical imaging of the treated region 24 hours postoperation P67
Fig.28 Histological observations on inflammatory cells and discrimination of eosinophils P68
-94-
|
| adam_txt |
Wang BG
Table of contents
Summary 1
1. Introduction 5
1. History and advances in laser refractive surgery 5
1.1 Radial keratotomy (RK) 5
1.2 Excimer laser-assisted photorefractive keratectomy (PRK) 6
1.3 Laser epithelial keratomileusis (LASEK) and Epi-LASIK 7
1.4 Laser-assisted in-situ keratomileusis (LASIK) 9
1.5Femto-LASIK(IntraLase) 12
1.6 New approaches being just under investigations 13
2. Histology of cornea (New Zealand Albino rabbits) 14
2.1 Description of cornea histology 15
2.1.1 Epithelium 15
2.1.2 Bowman's layer 16
2.1.3 Stroma 17
2.1.4 Descemet's layer 19
2.1.5 Endothelium 20
2.2 Corneal innervations 21
2.3 Factors maintaining corneal transparency 22
2.3.1 Collagen fibrils and lamellas 22
2.3.2 Stromal hydration 23
2.3.3 Function of endothelium 23
2.3.4 Other relevant factors 24
3. Mechanism and strategy of nanoprocessing in bulk tissue 24
4. Application of femtosecond lasers in biomedical fields 28
4.1 Diagnostics: Multiphoton microscopy 28
4.1.1 Two-photon autofluorescence (2PF) 29
4.1.2 Second harmonic generation (SHG) 30
4.2 Corneal Surgery 31
4.2.1 Intrastromal ablation with epithelial intact 31
4.2.2 Multiphoton-mediated generation of corneal flap and intrastromal
lenticule 32
2. Purposes of this work 34
3. Materials and Methods 35
1. Multiphoton microscope 35
2. Laser systems 36
2.1 Chameleon 36
2.2MaiTai 37
2.3 Coherent Vitesse 38
3. Threshold value for intrastromal nanosurgery and optimized parameters
for multiphoton microscopy 39
4. Animals, narcosis, and disinfection 40
5. Tissue-processing postoperation: specimen preparation, harvest and
analysis 41
4. Results 44
II
Wang BG
1. In-vivo corneal optical nonlinear imaging based on multiphoton
microscopy 44
1.1 Optical tomography of corneal tissue based on 2PF 44
1.1.1 Three-dimensional imaging of epithelial cells (three sublayers) 46
1.1.2 Three-dimensional imaging of stromal keratocytes 47
1.2 Optical tomography of stromal collagen lamellas based on SHG 47
1.3 Comparison of optical nonlinear images based on 2PF and SHG 48
2. In-vivo application of multiphoton imaging in viewing intrastromal
surgery 49
2.1 Evaluating intrastromal nanosurgery 50
2.2 Advantages of SHG in visualizing intrastromal surgery 50
2.2.1 Physiological architecture of collagen lamellas imaged with SHG 50
2.2.2 Observations of intrastromal surgery 24 hours postoperation 51
3. In-vivo intrastromal ablation with epithelium intact 53
3.1 Optical imaging during laser intrastromal ablation 53
3.2 Histological observations on intrastromal ablation (immediate results) 54
3.3 Follow-up on wound repair of intrastromal ablation 55
3.4 Cell activations in the treated cornea during wound healing process 56
4. In-vivo multiphoton-mediated generation of corneal flap 57
4.1 Optical observations during performing of corneal flaps 57
4.2 Immediate outcomes of corneal flaps 57
4.3 Follow-up on wound repair of the unlifted corneal flaps 58
5. Simultaneous generation of corneal flap and stromal lenticule 64
6. Observations on cells activation and migration in cornea during wound
repair 66
6.1 Optical tomography of activated keratocytes (myofibroblasts) 66
6.2 Histological observations: migrating inflammatory cells and discrimination
of eosinophils 67
5. Discussion, conclusions and outlook 70
1. Multiphoton tomography and nanoprocessing 70
1.1 Application of multiphoton microscopy in visualizing intrastromal surgery 70
1.2 Intrastromal ablation with epithelium intact 74
1.3 Multiphoton-mediated corneal flap generation 75
2. Wound repair after nanojoule ultrashort laser surgery in cornea 77
6. References 79
7. Appendix 91
8. List of figures and diagrams 93
9. Explanation (Selbstandigkeitserklarung) 95
10. Curriculum Vitae 96
11. Published and submitted works, Awards and advanced courses 97
12. Acknowledgments 100
III
Wang BG
List of Figures and Diagrams
Fig.1 Diagrams illustrating radial keratotomy (RK) P6
Fig.2 Diagrams illustrating PRK P6
Fig.3 Procedures of LASEK P8
Fig.4 Procedures of Epi-LASIK P9
Fig.4_1 Histological sections of Epi-LASIK P9
Fig.5 Procedures of LASIK P10
Fig.5_1 Photograph of microkeratome P10
Fig.5_2 Flap-related complications P11
Fig.6 Procedures of Femto-LASIK P12
Fig.6_1 Smooth flap bed generated with femtosecond high pulse energy lasers P13
Fig.7 All-Femto-LASIK P14
Fig.8_1 Imitated diagram of histological cross-section of rabbit cornea P16
Fig.8_2 Three-dimensional illustration of corneal components P16
Fig.8_3 TEM of corneal stroma P18
Fig.9 Diagram depicting noncentrosymmetric collagen structure P19
Fig.10 Nerve fibres in cornea displayed by gold chloride and optical imaging P22
Fig.11 Schematic of intrastromal surgery induced by nanojoule ultrashort laser pulses P25
Fig.12 Photographic illustration of animal laser eye surgery P35
Fig.12_1 Modified photograph of laser operations on rabbit P37
Fig.12_2 Schematic of experimental setup for multiphoton microscopy and intrastromal surgery. P38
Fig.12_3 Schematic illustrating intrastromal ablation P38
Fig.13 Threshold power for intratissue surgery and optimized power for multiphoton imaging P39
Fig.14 An indispensable instrument in this work: Zeissslit lamp 120 P42
Fig.14_1 Epithelial marks taken by slit lamp P42
Fig.15 Comparison of average central cornea thickness of rabbits, human and porcine P44
Fig.16_1 Nonlinear optical images of epithelial squamous cells and basal cells P45
Fig.16_2 Nonlinear optical images of keratocytes and endothelial cells P45
Fig.17_1 Optical nonlinear tomography of epithelium P46
Fig. 17_2 Optical nonlinear tomography of stromal keratocytes P47
Fig.17_3 In-vivo collagen lamellar nonlinear optical sectioning P48
Fig.18 Nonlinear optical imaging of stromal tissue with different wavelengths P49
Fig.19 Real-time nonlinear optical observations of intrastromal femtosecond laser nanosurgery P50
Fig.20 SHG image and A-scanning of emission wavelength P51
Fig.20_1 SHG observations on intrastromal nanosurgery 24 hours postoperation P51
Fig.20_2 Reflexion imaging of intrastromal nanosurgery 24 hours postoperation P52
Fig.21_1 On-line optical observations on intrastromal ablation P53
Fig.21_2 Histological cross-sections displaying the immediate ablation outcomes P54
-93-
Wang BG
Fig.21_3 Histological observations on wound repair of intrastromal ablation at 4th and 7th days P55
Fig.21_4 Optical detecting on emergence of myofibroblasts P56
Fig.22_1 Optical images during flap generation P57
Fig.22_2 Photographs of corneal flaps taken immediately after the laser operation P58
Fig.23SEM of the lifted corneal flap P58
Fig.24 Observations on wound repair at 1st day P59
Fig.24_1 Histological observations on the migrating inflammatory cells at 1st day P59
Fig.25_1 Histological observations of aflap at 7th day postoperation P60
Fig.25_2 Flap at 16th day P61
Fig.25_3 Flap at 28th day P62
Fig.25_4 Flap at 66th day P63
Fig.25_5 Flap at 90th day P64
Fig.26 Photograph showing simultaneous generation of corneal flap and intrastromal lenticule P65
Fig.27 In-vivo cellular nonlinear optical tomography of myofibroblasts based on 2PF P66
Fig.27_1 In-vivo optical imaging of the treated region 24 hours postoperation P67
Fig.28 Histological observations on inflammatory cells and discrimination of eosinophils P68
-94- |
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| index_date | 2024-07-02T16:37:57Z |
| indexdate | 2024-07-09T20:53:16Z |
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| language | English |
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| spelling | Wang, Bao-Gui 1973- Verfasser (DE-588)131940805 aut Multiphotonen-Mikroskopie und Laser-Nanochirurgie der Kornea mittels Naher-Infrarot Nanojoule Femtosekunden Laserpulse = Animal studies on Cornea nanosurgery and multiphoton microscopy with nanojoule femtosecond near-infrared lasers Bao-Gui Wang Animal studies on Cornea nanosurgery and multiphoton microscopy with nanojoule femtosecond near-infrared lasers 2006 III, 101 Bl. Ill. txt rdacontent n rdamedia nc rdacarrier Zsfassung in dt. Sprache. - Jena, Univ., Diss., 2006 (DE-588)4113937-9 Hochschulschrift gnd-content HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=015457562&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Wang, Bao-Gui 1973- Multiphotonen-Mikroskopie und Laser-Nanochirurgie der Kornea mittels Naher-Infrarot Nanojoule Femtosekunden Laserpulse = Animal studies on Cornea nanosurgery and multiphoton microscopy with nanojoule femtosecond near-infrared lasers |
| subject_GND | (DE-588)4113937-9 |
| title | Multiphotonen-Mikroskopie und Laser-Nanochirurgie der Kornea mittels Naher-Infrarot Nanojoule Femtosekunden Laserpulse = Animal studies on Cornea nanosurgery and multiphoton microscopy with nanojoule femtosecond near-infrared lasers |
| title_alt | Animal studies on Cornea nanosurgery and multiphoton microscopy with nanojoule femtosecond near-infrared lasers |
| title_auth | Multiphotonen-Mikroskopie und Laser-Nanochirurgie der Kornea mittels Naher-Infrarot Nanojoule Femtosekunden Laserpulse = Animal studies on Cornea nanosurgery and multiphoton microscopy with nanojoule femtosecond near-infrared lasers |
| title_exact_search | Multiphotonen-Mikroskopie und Laser-Nanochirurgie der Kornea mittels Naher-Infrarot Nanojoule Femtosekunden Laserpulse = Animal studies on Cornea nanosurgery and multiphoton microscopy with nanojoule femtosecond near-infrared lasers |
| title_exact_search_txtP | Multiphotonen-Mikroskopie und Laser-Nanochirurgie der Kornea mittels Naher-Infrarot Nanojoule Femtosekunden Laserpulse = Animal studies on Cornea nanosurgery and multiphoton microscopy with nanojoule femtosecond near-infrared lasers |
| title_full | Multiphotonen-Mikroskopie und Laser-Nanochirurgie der Kornea mittels Naher-Infrarot Nanojoule Femtosekunden Laserpulse = Animal studies on Cornea nanosurgery and multiphoton microscopy with nanojoule femtosecond near-infrared lasers Bao-Gui Wang |
| title_fullStr | Multiphotonen-Mikroskopie und Laser-Nanochirurgie der Kornea mittels Naher-Infrarot Nanojoule Femtosekunden Laserpulse = Animal studies on Cornea nanosurgery and multiphoton microscopy with nanojoule femtosecond near-infrared lasers Bao-Gui Wang |
| title_full_unstemmed | Multiphotonen-Mikroskopie und Laser-Nanochirurgie der Kornea mittels Naher-Infrarot Nanojoule Femtosekunden Laserpulse = Animal studies on Cornea nanosurgery and multiphoton microscopy with nanojoule femtosecond near-infrared lasers Bao-Gui Wang |
| title_short | Multiphotonen-Mikroskopie und Laser-Nanochirurgie der Kornea mittels Naher-Infrarot Nanojoule Femtosekunden Laserpulse |
| title_sort | multiphotonen mikroskopie und laser nanochirurgie der kornea mittels naher infrarot nanojoule femtosekunden laserpulse animal studies on cornea nanosurgery and multiphoton microscopy with nanojoule femtosecond near infrared lasers |
| title_sub | = Animal studies on Cornea nanosurgery and multiphoton microscopy with nanojoule femtosecond near-infrared lasers |
| topic_facet | Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=015457562&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT wangbaogui multiphotonenmikroskopieundlasernanochirurgiederkorneamittelsnaherinfrarotnanojoulefemtosekundenlaserpulseanimalstudiesoncorneananosurgeryandmultiphotonmicroscopywithnanojoulefemtosecondnearinfraredlasers AT wangbaogui animalstudiesoncorneananosurgeryandmultiphotonmicroscopywithnanojoulefemtosecondnearinfraredlasers |