The glucocorticoid receptor DNA binding domain: structure and allosteric modulation = Het DNA bindend domein van de glucocorticoide receptor
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| Format: | Thesis Book |
| Language: | Undetermined |
| Published: |
1998
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| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Physical Description: | 111 S. graph. Darst. |
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MARC
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| 100 | 1 | |a Tilborg, Marc van |e Verfasser |4 aut | |
| 245 | 1 | 0 | |a The glucocorticoid receptor DNA binding domain |b structure and allosteric modulation = Het DNA bindend domein van de glucocorticoide receptor |c door Marcus Adrianus Antonius van Tilborg |
| 246 | 1 | 1 | |a Het DNA bindend domein van de glucocorticoide receptor |
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| _version_ | 1804144645545918464 |
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| adam_text | IMAGE 1
THE GLUCOCORTICOID RECEPTOR
DNA BINDING DOMAIN
STRUCTURE AND ALLOSTERIC MODULATION
UB BRAUNSCHWEIG 84
4545-584-3
IMAGE 2
CONTENTS :
CHAPTER 1 THE NUCLEAR RECEPTORS. AN OVERVIEW.
L.L 1.2
1.3
1.4
1.5
1.6
1.7
1.8
1.9
GENERAL INTRODUCTION
ARCHITECTURE OF THE NUCLEAR RECEPTORS
DNA BINDING DOMAIN
LIGAND BINDING DOMAIN
CO-ACTIVATORS AND CO-REPRESSORS
ORPHAN RECEPTORS
STEROIDS: BIOLOGY AND CLINICAL RELEVANCE
SUMMARY AND PERSPECTIVES
AIM OF THIS THESIS
REFERENCES
2.1 2.2
2.3
2.4
INTRODUCTION
MUTANTS OF THE GR DBD
S459A AND P493R
K461A MUTANT
MATERIAL AND METHODS
RESULTS AND DISCUSSION
REFERENCES
10 11
13
20
24
28
29
34
34
35
CHAPTER 2
PROPERTIES OF WILD-TYPE GLUCOCORTICOID RECEPTOR DNA BINDING DOMAIN AND
THE MUTANTS S459A, P493R AND K461A. 41
42 44
44
48
49
51
55
CHAPTER 3
STRUCTURE REFINEMENT OF THE GLUCOCORTICOID RECEPTOR-DNA BINDING DOMAIN
FROM NMR DATA BY RELAXATION MATRIX CALCULATIONS 57
3.1 INTRODUCTION 58
IMAGE 3
3.2 MATERIALS AND METHODS 60
SAMPLE PREPARATION 60
NMR-EXPERIMENTS 60
DISTANCE CONSTRAINTS 61
RELAXATION MATRIX CALCULATIONS 63
STRUCTURE REFINEMENT PROCEDURE 64
3.3 RESULTS AND DISCUSSION 65
GENERALIZED ORDER PARAMENTER S^ 65
STRUCTURE REFINEMENT 66
ANALYSIS OF THE STRUCTURE 70
SOLUTION STRUCTURE VS X-RAY STRUCTURE 72
DISORDER IN SECOND ZINC FINGER 74
3.4 CONCLUSIONS 76
ACKNOWLEDGEMENTS 77
REFERENCES 77
CHAPTER 4
THE MUTANTS S459A AND P493R IN THE GLUCOCORTICOID RECEPTOR DBD MIMIC THE
ALLOSTERIC EFFECT OF DNA 81
4.1 4.2
4.3
4.4
SUMMARY
SAMENVATTING
DANKWOORD
INTRODUCTION MATERIALS AND METHODS
RESULTS AND DISCUSSION
4.2.1 STRUCTURE DETERMINATION
4.2.2 ANALYSIS OF THE STRUCTURES
4.2.3 MECHANISM FOR STRUCTURAL CHANGES
FREE AND DNA BOUND GR DBD
THE MUTANTS S459A AND P493R
CONCLUSIONS
ACKNOWLEDGEMENTS
REFERENCES
CURRICULUM VITAE
82 84
86
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92
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98
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111
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| spelling | Tilborg, Marc van Verfasser aut The glucocorticoid receptor DNA binding domain structure and allosteric modulation = Het DNA bindend domein van de glucocorticoide receptor door Marcus Adrianus Antonius van Tilborg Het DNA bindend domein van de glucocorticoide receptor 1998 111 S. graph. Darst. txt rdacontent n rdamedia nc rdacarrier Utrecht, Univ., Diss., 1998 (DE-588)4113937-9 Hochschulschrift gnd-content GBV Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=021772265&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Tilborg, Marc van The glucocorticoid receptor DNA binding domain structure and allosteric modulation = Het DNA bindend domein van de glucocorticoide receptor |
| subject_GND | (DE-588)4113937-9 |
| title | The glucocorticoid receptor DNA binding domain structure and allosteric modulation = Het DNA bindend domein van de glucocorticoide receptor |
| title_alt | Het DNA bindend domein van de glucocorticoide receptor |
| title_auth | The glucocorticoid receptor DNA binding domain structure and allosteric modulation = Het DNA bindend domein van de glucocorticoide receptor |
| title_exact_search | The glucocorticoid receptor DNA binding domain structure and allosteric modulation = Het DNA bindend domein van de glucocorticoide receptor |
| title_full | The glucocorticoid receptor DNA binding domain structure and allosteric modulation = Het DNA bindend domein van de glucocorticoide receptor door Marcus Adrianus Antonius van Tilborg |
| title_fullStr | The glucocorticoid receptor DNA binding domain structure and allosteric modulation = Het DNA bindend domein van de glucocorticoide receptor door Marcus Adrianus Antonius van Tilborg |
| title_full_unstemmed | The glucocorticoid receptor DNA binding domain structure and allosteric modulation = Het DNA bindend domein van de glucocorticoide receptor door Marcus Adrianus Antonius van Tilborg |
| title_short | The glucocorticoid receptor DNA binding domain |
| title_sort | the glucocorticoid receptor dna binding domain structure and allosteric modulation het dna bindend domein van de glucocorticoide receptor |
| title_sub | structure and allosteric modulation = Het DNA bindend domein van de glucocorticoide receptor |
| topic_facet | Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=021772265&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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