Procollagen C-proteinase enhancers in skin wound healing: expression, functions and therapeutic potential
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
Freiburg im Breisgau ; Lyon
29/06/2018
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis Inhaltsverzeichnis |
| Beschreibung: | 310 Seiten Illustrationen, Diagramme 30 cm |
Internformat
MARC
| LEADER | 00000nam a2200000 c 4500 | ||
|---|---|---|---|
| 001 | BV045485485 | ||
| 003 | DE-604 | ||
| 005 | 00000000000000.0 | ||
| 007 | t | ||
| 008 | 190225s2018 gw a||| m||| 00||| eng d | ||
| 015 | |a 18,H12 |2 dnb | ||
| 016 | 7 | |a 1170192785 |2 DE-101 | |
| 020 | |c Broschur | ||
| 035 | |a (OCoLC)1090104826 | ||
| 035 | |a (DE-599)BSZ51228122X | ||
| 040 | |a DE-604 |b ger |e rda | ||
| 041 | 0 | |a eng | |
| 044 | |a gw |c XA-DE | ||
| 049 | |a DE-355 | ||
| 084 | |a 610 |2 sdnb | ||
| 084 | |a 570 |2 sdnb | ||
| 100 | 1 | |a Tessier, Agnès |e Verfasser |0 (DE-588)1169842550 |4 aut | |
| 245 | 1 | 0 | |a Procollagen C-proteinase enhancers in skin wound healing |b expression, functions and therapeutic potential |c Angès Tessier |
| 264 | 1 | |a Freiburg im Breisgau ; Lyon |c 29/06/2018 | |
| 300 | |a 310 Seiten |b Illustrationen, Diagramme |c 30 cm | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 502 | |b Dissertation |c Université Claude Bernard Lyon 1 |d 2018 | ||
| 655 | 7 | |0 (DE-588)4113937-9 |a Hochschulschrift |2 gnd-content | |
| 856 | 4 | 2 | |m B:DE-101 |q application/pdf |u http://d-nb.info/1170192785/04 |3 Inhaltsverzeichnis |
| 856 | 4 | 2 | |m DNB Datenaustausch |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=030870448&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
| 999 | |a oai:aleph.bib-bvb.de:BVB01-030870448 | ||
Datensatz im Suchindex
| _version_ | 1804179406792425472 |
|---|---|
| adam_text | TABLE O F CONTENTS
ABSTRACT...........................................................................................................................................
9
RESUME
SUBSTANTIEL........................................................................................................................10
TABLE OF CONTENTS
..........................................
13
LIST OF
FIGURES................................................................................................................................
19
LIST OF
TABLES..................................................................................................................................22
ABBREVIATIONS................................................................................................................................23
C HAPTER 1:
INTRODUCTION.............................................................................................................29
I. SKIN: STRUCTURE AND
FUNCTIONS...........................................................................................
31
1) GENERAL
DESCRIPTION.......................................................................................................31
2)
EPIDERMIS.....................................................................................................................
32
3) DERMAL-EPIDERMAL
JUNCTION.........................................................................................
34
4)
DERMIS..........................................................................................................................
35
A. DERMAL EXTRACELLULAR M
ATRIX..................................................................................
36
B. RESIDENT CELLS OF THE DERM IS
.........
........................................................................
37
5)
HYPODERMIS..................................................................................................................
38
6) DIFFERENCES BETWEEN MOUSE AND HUMAN
SKIN............................................................. 40
A. STRUCTURE AND GENERAL
CHARACTERISTICS....................................................................
40
B. IMMUNOLOGY
.......
.
..................................................................................................40
II. SKIN WOUND
HEALING.........................................................................................................
42
1) HAEMOSTASIS AND INFLAMMATORY PHASE (0-3 DAYS)
.......................................................
42
2) PROLIFERATIVE PHASE (4-21
DAYS)...................................................................................44
3) REMODELLING PHASE (21 DAYS-1
YEAR)...........................................................................44
4) PATHOLOGICAL WOUND
HEALING........................................................................................
45
A. CHRONIC
WOUNDS.....................................................................................................
45
B. HYPERTROPHIC SCARS AND
KELOIDS.............................................................................46
III. COLLAGEN
FIBRILLOGENESIS...............................................................................................
48
1) THE COLLAGEN
FAMILY......................................................................................................
48
2) FIBRILLAR COLLAGEN
BIOSYNTHESIS....................................................................................49
3) BMP-1 /TOLLOID-LIKE PROTEINASES
........
.
........................................................................
52
A. IDENTIFICATION AND
STRUCTURE.....................................................................................
52
B. DIRECT ROLES IN COLLAGEN FIBRIL FORMATION AND
ASSEMBLY.........................................54
C. BASEMENT MEMBRANE ASSEMBLY
.............................................................................
57
D. ACTIVATION OF GROWTH FACTORS
.................................................................................
58
E.
REGULATORS................................................................................................................59
F. PATHOPHYSIOLOGICAL ROLES OF
BTPS...........................................................................
60
IV. PROCOLLAGEN C-PROTEINASE ENHANCERS (PCPES)
............................................................
62
1) IDENTIFICATION OF
PCPES.................................................................................................
62
2) FUNCTION AND
SPECIFICITY...............................................................................................
62
3) STRUCTURE OF
PCPES.......................................................................................................
65
A. CUB
DOMAINS.........................................................................................................
66
B. NTR
DOMAIN............................................................................................................66
C. DIFFERENCES BETWEEN PCPE-1 AND -2 PROTEIN
STRUCTURES.......................................67
4) MECHANISM OF
ACTION....................................................................................................
68
A. DIRECT INTERACTION BETWEEN PCPE AND
PROCOLLAGEN.............................................. 68
B. SUPERSTIMULATION BY HSPGS
..................................................................................
70
5) DIFFERENTIAL EXPRESSION, DISTRIBUTION AND IN VIVO ROLES
...............................................
71
A. EXPRESSION AMONG
SPECIES................................................... 71
B. EXPRESSION AND DISTRIBUTION IN
TISSUES...................................................................72
C. IN VIVO
FUNCTIONS.....................................................................................................
73
I. BONE DEVELOPMENT AND
HOMEOSTASIS.....................................................................73
II. LIPID
METABOLISM................................................................................................
74
III.
FIBROSIS................................................................................................................74
IV. WOUND
HEALING....................................................................................................
76
OBJECTIVES......................................................................................................................................78
C HAPTER 2: M ATERIALS AND M
ETHODS...........................................................................................79
I. PRODUCTION AND PURIFICATION OF RECOMBINANT PROTEINS
.....................................................
81
1) VECTOR
CLONING................................................................................................................81
A. PCPE-2 IN PCEP4 EXPRESSION
VECTOR....................................................................81
I. POTB7-PCPE-2 AMPLIFICATION
..............................................................................
81
II. PCPE-2 SEQUENCE MUTAGENESIS AND CLONING INTO PCEP4 VECTOR
........................
81
B. PCPE-2-8HIS IN PCEP4 EXPRESSION VECTOR
...........................................................
82
C. 6HIS-PCPE2 IN PHLSEC
VECTOR.............................................................................
83
2) CULTURE AND TRANSFECTION OF EUKARYOTIC CELL LINES
........................................................
83
A. CULTURE OF HEK 293-EBNA AND HER 293-T
CELLS................................................83
B. STABLE TRANSFECTION AND PRODUCTION OF PCPE-1, PCPE-L-8HIS, PCPE-2 AND
PCPE-
2-8HIS
..............................................................................................................................
84
C. TRANSIENT TRANSFECTION AND PRODUCTION OF
6HIS-PCPE-2.......................................85
3) PROTEIN
PURIFICATION.......................................................................................................85
A.
AFFI-BLUE................................................................................................................
85
B.
NI-NTA..................................................................................................................
86
C.
HEPARIN...................................................................................................................
87
D.
DIALYSIS...................................................................................................................
89
E. SUPERDEX
200..........................................................................................................
89
4) PROTEIN
ANALYSIS............................................................................................................90
II. CELL
CULTURE......................................................................................................................
90
1) CULTURE OF PRIMARY KERATINOCYTES AND DERMAL FIBROBLASTS
............................................
90
A. PRIMARY CELLS ISOLATED FROM MOUSE SKIN
...............................................................
90
B. PRIMARY CELLS ISOLATED FROM HUMAN SKIN
...............................................................
90
C. SUBCULTURE OF PRIMARY
CELLS...................................................................................
91
D.
FREEZING..................................................................................................................
91
2) DIFFERENTIATION INTO MYOFIBROBLASTS BY TGFSS
TREATMENT.................................. 92
3) INHIBITION OF PCPE-1 IN FIBROBLASTS BY RNA
INTERFERENCE.........................................92
A. TRANSFECTION WITH
SIRNA......................................................................................
92
B. INFECTION WITH
SHRNA-LENTIVIRUS...........................................................................92
4) KERATINOCYTE
DIFFERENTIATION........................................................................................
93
5) CULTURE OF MONOCYTE AND MACROPHAGES CELL
LINES....................................................... 94
A. SUBCULTURE OF THP-1 AND U937 CELLS
...................................................................
94
B. DIFFERENTIATION AND POLARIZATION OF
MACROPHAGES.................................................94
I. DIFFERENTIATION OF MONOCYTES INTO M0
MACROPHAGES............................................94
II. M L OR M2 MACROPHAGE POLARIZATION
.................................................................
94
III. IN VITRO CLEAVAGE
ASSAYS...............................................................................................
95
1) CLEAVAGE ASSAYS WITH RECOMBINANT
SUBSTRATES..............................................................95
A. PROCOLLAGEN
III........................................................................................................95
B. PROCOLLAGEN V
.........................................................................................................95
C.
CHORDIN...................................................................................................................95
D.
BETAGLYCAN..............................................................................................................96
E.
THROMBOSPONDIN-1................................................................................................
96
2) CLEAVAGE ASSAYS WITH CELL CONDITIONED
MEDIA............................................................ 96
IV. MURINE WOUND HEALING M
ODEL.....................................................................................
97
15
1) MOUSE M
ODELS................................................................................................................97
2) ASSESSMENT OF WOUND
HEALING......................................................................................97
V. TRANSCRIPTIONAL
ANALYSES...................................................................................................
98
1) RNA
EXTRACTION.............................................................................................................98
2) PC R
...............................................................................................................................
98
3)
QRT-PCR.......................................................................................................................
99
VI. PROTEIN
ANALYSES...........................................................................................................101
1)
IMMUNOBLOTTING...........................................................................................................
101
A. SAMPLE
PREPARATION...............................................................................................
101
I. CELL CONDITIONED
MEDIUM.....................................................................................
101
II. ECM
EXTRACTION..................................................................................................101
III. CELL
LYSATES.........................................................................................................101
IV. SKIN
LYSATES........................................................................................................102
B. WESTERN
BLOTTING..................................................................................................
102
2)
IMMUNOFLUORESCENCE..................................................................................................
104
A. CELL
CULTURES...........................................................................................................104
B. TISSUES
......
............................................................................................................
104
3) FLOW
CYTOMETRY...........................................................................................................105
A.
CELLS.........................................................................
105
B. MOUSE
SKIN............................................................................................................105
4) MASS
SPECTROMETRY.......................................................................................................106
A. PROTEIN SAMPLE
PREPARATION...................................................................................106
B. TMT* LABELLING FOR RELATIVE QUANTITATION BY MASS SPECTROMETRY
.....................
106
C. MS/MS DATA
ANALYSIS............................................................................................
107
CHAPTER 3: EXPRESSION OF BMP-L/TOLLOID-LIKE PROTEINASES (BTPS) AND
PROCOLLAGEN C-
PROTEINASE ENHANCERS (PCPES) IN
SKIN.................................................................................
109
I. MOUSE SKIN WOUND HEALING
MODEL..................................................................................I
L L
1) EXPRESSION OF BTPS DURING SKIN WOUND
HEALING.......................................................112
2) PCPE EXPRESSION PATTERNS DURING SKIN WOUND HEALING
.............................................
113
II. PATHOLOGICAL HUMAN
WOUNDS...........................................................................................
119
1) BMP- 1/MTLD EXPRESSION IN PATHOLOGICAL
WOUNDS....................................................119
2) EXPRESSION OF PCPE-2 IN HUMAN PATHOLOGICAL WOUNDS
............................................
121
III. EXPRESSION OF BTPS AND PCPES IN SKIN
CELLS............................................................124
1) KERATINOCYTES AND
FIBROBLASTS......................................................................................
124
A.
BTPS.......................................................................................................................
124
B.
PCPE-1..................................................................................................................125
C. PCPE-2
.................................................................................
127
2) IMMUNE
CELLS................................................................................................................131
A. PCPE-2 EXPRESSION IN MOUSE
SKIN.......................................................................
131
B. PCPE-2 EXPRESSION IN HUMAN
SKIN......................................................................
133
C. PCPE-2 EXPRESSION IN CULTURED MONOCYTES AND MACROPHAGES
..........................
133
IV.
DISCUSSION....................................................................................................................137
1) BTPS AND PCPE-1 ARE UPREGULATED IN THE PROLIFERATION PHASE OF WOUND
HEALING .. 137
2) PCPE-2 MIGHT PLAY A DIFFERENT ROLE DURING THE INFLAMMATORY PHASE
.......................
137
CHAPTER 4: ROLE OF PCPE-1 IN FIBROBLASTS............................ 143
I.
INTRODUCTION.....................................................................................................................145
II. EFFECT OF TGFSS ON PCPE-1
EXPRESSION........................................................................146
III. EFFECTS OF PCPE-1 INHIBITION IN
FIBROBLASTS...............................................................147
1) INHIBITION OF PCPE-1 AND CELL
GROWTH.......................................................................
147
A. SMALL INTERFERING RNA
(SIRNA).........................................................................
147
B. SHORT HAIRPIN RNA
(SHRNA)...............................................................................
148
C. DARPINS............................. 149
2) C-TERMINAL PROCESSING OF TYPE I
PROCOLLAGEN.............................................................150
3) PROTEIN
SYNTHESIS.........................................................................................................
154
A. SAMPLE PREPARATION AND TMT
LABELLING..............................................................154
B. PROTEIN IDENTIFICATION AND
ANNOTATION..................................................................156
C. PROTEIN
QUANTIFICATION...........................................................................................157
IV.
DISCUSSION..................................................................................................................
159
1) EFFECT OF TGFSS ON PCPE-1
EXPRESSION......................................................................159
2) SPECIFICITY OF PCPE-1 FOR PROCOLLAGEN PROCESSING
...................................................
159
C HAPTER 5: FUNCTIONAL CHARACTERIZATION OF THE PCPE-2 P
ROTEIN........................................163
I.
INTRODUCTION....................................................................................................................
165
II. PURIFICATION OF RECOMBINANT
PROTEINS............................................................................166
1) PCPE-1 PROTEIN AND ITS TAGGED-FORM, PCPE-L-8HIS
.................................................
166
A.
PCPE-1.................................................................................................................
166
B.
PCPE-L-8HIS........................................................................................................167
2) PCPE-2 PROTEINS: PCPE-2, PCPE-2-8HIS AND
6-HIS-PCPE-2.................................168
A.
PCPE-2.................................................................................................................
168
B.
PCPE-2-8HIS........................................................................................................170
17
C.
6HIS-PCPE-2..........................................................................................................171
III. IN VITRO CLEAVAGE ASSAYS
..............................................................................................
174
1) FIBRILLAR
PROCOLLAGENS.................................................................................................
174
A. EFFECT OF PCPE-2 ON TYPE I
PROCOLLAGEN..............................................................
175
B. EFFECT OF PCPE-2 ON TYPE II
PROCOLLAGEN............................................................176
C. EFFECT OF PCPE-2 ON TYPE III
PROCOLLAGEN...........................................................177
D. EFFECT OF PCPE-2 ON TYPE V
PROCOLLAGEN............................................................179
2) NON COLLAGENOUS SUBSTRATES OF BMP-1
......................................................................
181
3) DIRECT EFFECT OF PCPE-2 ON BMP-1
ACTIVITY................................................................184
IV.
DISCUSSION...................................................................................................................
185
C HAPTER 6: PCOLCE2 KNOCKOUT M
ICE.........................................................................................
189
I. VALIDATION OF THE PCOLCE2
KNOCKOUT...............................................................................191
II. CHARACTERIZATION OF THE
PCOLCE2 KO SKIN
......................................................................
194
1) STRUCTURE OF THE
SKIN....................................................................................................194
2) MOLECULAR
CHANGES.......................................................................................................194
A. EXPRESSION OF PCPE-1 AND B TPS
........................................................................
194
B. EXPRESSION OF OTHER
MARKERS.................................................................................195
III. WOUND HEALING
ASSAY...................................................................................................196
1) WOUND
CLOSURE.............................................................................................................197
2)
RE-EPITHELIALIZATION.....................................................................................................198
3) INFILTRATION OF IMMUNE
CELLS........................................................................................
199
4) A-SMA
EXPRESSION......................................................... 200
IV.
DISCUSSION...................................................................................................................202
1) TECHNICAL ISSUES
ENCOUNTERED....................................................................................
202
2) CONSEQUENCES OF THE ABSENCE OF PCPE-2 IN SKIN
.....................................................
202
GENERAL DISCUSSION AND
CONCLUSION.........................................................................................207
BIBLIOGRAPHY................................................................................................................................
215
APPENDIXES..................................................................................................................................
229
COMMUNICATION &
TRAINING.......................................................................................................
239
LIST OF
PUBLICATIONS......................................................................................................................
241
ORAL
COMMUNICATIONS..................................................................................................................288
POSTERS..........................................................................................................................................305
FUNDING AND
GRANTS......................................................................................................................
310
TRAINING........................................................................................................................................310
! 8
|
| any_adam_object | 1 |
| author | Tessier, Agnès |
| author_GND | (DE-588)1169842550 |
| author_facet | Tessier, Agnès |
| author_role | aut |
| author_sort | Tessier, Agnès |
| author_variant | a t at |
| building | Verbundindex |
| bvnumber | BV045485485 |
| ctrlnum | (OCoLC)1090104826 (DE-599)BSZ51228122X |
| discipline | Biologie Medizin |
| format | Thesis Book |
| fullrecord | <?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01419nam a2200361 c 4500</leader><controlfield tag="001">BV045485485</controlfield><controlfield tag="003">DE-604</controlfield><controlfield tag="005">00000000000000.0</controlfield><controlfield tag="007">t</controlfield><controlfield tag="008">190225s2018 gw a||| m||| 00||| eng d</controlfield><datafield tag="015" ind1=" " ind2=" "><subfield code="a">18,H12</subfield><subfield code="2">dnb</subfield></datafield><datafield tag="016" ind1="7" ind2=" "><subfield code="a">1170192785</subfield><subfield code="2">DE-101</subfield></datafield><datafield tag="020" ind1=" " ind2=" "><subfield code="c">Broschur</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(OCoLC)1090104826</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)BSZ51228122X</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-604</subfield><subfield code="b">ger</subfield><subfield code="e">rda</subfield></datafield><datafield tag="041" ind1="0" ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="044" ind1=" " ind2=" "><subfield code="a">gw</subfield><subfield code="c">XA-DE</subfield></datafield><datafield tag="049" ind1=" " ind2=" "><subfield code="a">DE-355</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">610</subfield><subfield code="2">sdnb</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">570</subfield><subfield code="2">sdnb</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Tessier, Agnès</subfield><subfield code="e">Verfasser</subfield><subfield code="0">(DE-588)1169842550</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Procollagen C-proteinase enhancers in skin wound healing</subfield><subfield code="b">expression, functions and therapeutic potential</subfield><subfield code="c">Angès Tessier</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="a">Freiburg im Breisgau ; Lyon</subfield><subfield code="c">29/06/2018</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">310 Seiten</subfield><subfield code="b">Illustrationen, Diagramme</subfield><subfield code="c">30 cm</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="b">n</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="b">nc</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="502" ind1=" " ind2=" "><subfield code="b">Dissertation</subfield><subfield code="c">Université Claude Bernard Lyon 1</subfield><subfield code="d">2018</subfield></datafield><datafield tag="655" ind1=" " ind2="7"><subfield code="0">(DE-588)4113937-9</subfield><subfield code="a">Hochschulschrift</subfield><subfield code="2">gnd-content</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="m">B:DE-101</subfield><subfield code="q">application/pdf</subfield><subfield code="u">http://d-nb.info/1170192785/04</subfield><subfield code="3">Inhaltsverzeichnis</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="m">DNB Datenaustausch</subfield><subfield code="q">application/pdf</subfield><subfield code="u">http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=030870448&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA</subfield><subfield code="3">Inhaltsverzeichnis</subfield></datafield><datafield tag="999" ind1=" " ind2=" "><subfield code="a">oai:aleph.bib-bvb.de:BVB01-030870448</subfield></datafield></record></collection> |
| genre | (DE-588)4113937-9 Hochschulschrift gnd-content |
| genre_facet | Hochschulschrift |
| id | DE-604.BV045485485 |
| illustrated | Illustrated |
| indexdate | 2024-07-10T08:19:22Z |
| institution | BVB |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-030870448 |
| oclc_num | 1090104826 |
| open_access_boolean | |
| owner | DE-355 DE-BY-UBR |
| owner_facet | DE-355 DE-BY-UBR |
| physical | 310 Seiten Illustrationen, Diagramme 30 cm |
| publishDate | 2018 |
| publishDateSearch | 2018 |
| publishDateSort | 2018 |
| record_format | marc |
| spelling | Tessier, Agnès Verfasser (DE-588)1169842550 aut Procollagen C-proteinase enhancers in skin wound healing expression, functions and therapeutic potential Angès Tessier Freiburg im Breisgau ; Lyon 29/06/2018 310 Seiten Illustrationen, Diagramme 30 cm txt rdacontent n rdamedia nc rdacarrier Dissertation Université Claude Bernard Lyon 1 2018 (DE-588)4113937-9 Hochschulschrift gnd-content B:DE-101 application/pdf http://d-nb.info/1170192785/04 Inhaltsverzeichnis DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=030870448&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Tessier, Agnès Procollagen C-proteinase enhancers in skin wound healing expression, functions and therapeutic potential |
| subject_GND | (DE-588)4113937-9 |
| title | Procollagen C-proteinase enhancers in skin wound healing expression, functions and therapeutic potential |
| title_auth | Procollagen C-proteinase enhancers in skin wound healing expression, functions and therapeutic potential |
| title_exact_search | Procollagen C-proteinase enhancers in skin wound healing expression, functions and therapeutic potential |
| title_full | Procollagen C-proteinase enhancers in skin wound healing expression, functions and therapeutic potential Angès Tessier |
| title_fullStr | Procollagen C-proteinase enhancers in skin wound healing expression, functions and therapeutic potential Angès Tessier |
| title_full_unstemmed | Procollagen C-proteinase enhancers in skin wound healing expression, functions and therapeutic potential Angès Tessier |
| title_short | Procollagen C-proteinase enhancers in skin wound healing |
| title_sort | procollagen c proteinase enhancers in skin wound healing expression functions and therapeutic potential |
| title_sub | expression, functions and therapeutic potential |
| topic_facet | Hochschulschrift |
| url | http://d-nb.info/1170192785/04 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=030870448&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT tessieragnes procollagencproteinaseenhancersinskinwoundhealingexpressionfunctionsandtherapeuticpotential |
Es ist kein Print-Exemplar vorhanden.
Inhaltsverzeichnis