Handbook for rhizobia: methods in legume rhizobium technology
Gespeichert in:
| Hauptverfasser: | , |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
New York u.a.
Springer
1994
|
| Schriftenreihe: | Springer laboratory
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | Literaturverz. S. 439 - 441 |
| Beschreibung: | XVI, 450 S. Ill., graph. Darst. |
| ISBN: | 3540941347 0387941347 |
Internformat
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Datensatz im Suchindex
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|---|---|
| adam_text | P. SOMASEGARAN H.J. HOBEN HANDBOOK FOR RHIZOBIA METHODS IN
LEGUME-RHIZOBIUM TECHNOLOGY WITH 85 ILLUSTRATIONS SPRINGER-VERLAG NEW
YORK BERLIN HEIDELBERG LONDON PARIS TOKYO HONG KONG BARCELONA BUDAPEST
CONTENTS FOREWORD V FOREWORD TO NIFTAL EDITION VII PREFACE IX
ACKNOWLEDGMENTS XI I. GENERAL MICROBIOLOGY OF RHIZOBIA 1 1. COLLECTING
NODULES AND ISOLATING RHIZOBIA 7 2. OBSERVING THE INFECTION PROCESS 24
3. CULTURAL PROPERTIES, CELL MORPHOLOGY, AND NUTRITIONAL REQUIREMENTS OF
RHIZOBIA 31 4. DEMONSTRATING GENETIC DIVERSITY IN RHIZOBIA USING
PATTERNS OF CARBOHYDRATE UTILIZATION AND INTRINSIC ANTIBIOTIC RESISTANCE
38 5. QUANTIFYING THE GROWTH OF RHIZOBIA 47 6. COUNTING RHIZOBIA BY A
PLANT INFECTION METHOD 58 7. COUNTING SEROLOGICALLY SPECIFIC RHIZOBIA IN
SOIL AND PEAT INOCULANTS USING MEMBRANE FILTERS AND IMMUNOFLUORESCENCE
65 ADDITIONAL REFERENCES AND RECOMMENDED READING 75 II. IDENTIFICATION
OF RHIZOBIA 79 8. DEVELOPING ANTISERA 89 9. SOMATIC AGGLUTINATION
REACTIONS WITH PURE CULTURES OF RHIZOBIA 94 10. AGGLUTINATING ANTIGENS
FROM ROOT NODULES 102 11. PERFORMING RHIZOBIAL ANTIGEN-ANTIBODY
REACTIONS BY GEL IMMUNODIFFUSION 107 XIV CONTENTS 12. DETERMINING STRAIN
OCCUPANCY IN SOYBEAN NODULES BY GEL IMMUNODIFFUSION 112 13. PRODUCING
AND APPLYING FLUORESCENT ANTIBODIES 120 14. IDENTIFYING RHIZOBIA BY THE
INDIRECT ENZYME-LINKED IMMUNOSORBENT ASSAY 131 15. IDENTIFYING RHIZOBIA
BY IMMUNOBLOT 140 16. ISOLATING SPONTANEOUS ANTIBIOTIC-RESISTANT MUTANTS
OF RHIZOBIA 149 17. ANALYZING NODULE OCCUPANCY USING
ANTIBIOTIC-RESISTANT MARKERS 153 18. DISTINGUISHING BETWEEN STRAINS OF
RHIZOBIA BY RHIZOBIOPHAGE SUSCEPTIBILITY 158 ADDITIONAL REFERENCES AND
RECOMMENDED READING 163 III. EVALUATING SYMBIOTIC POTENTIAL OF RHIZOBIA
165 19. TESTING FOR GENETIC COMPATIBILITY BETWEEN RHIZOBIA AND LEGUMES
171 20. SCREENING RHIZOBIA FOR NITROGEN-FIXATION POTENTIAL 177 21.
SCREENING EFFECTIVE STRAINS OF RHIZOBIA IN POTTED FIELD SOIL 182 22.
VERIFYING THE NITROGEN-FIXING POTENTIAL OF GLASSHOUSE- SELECTED SOYBEAN
RHIZOBIA IN THE FIELD ENVIRONMENT 189 23. EVALUATING THE SYMBIOTIC
POTENTIAL OF INDIGENOUS RHIZOBIAL POPULATIONS OF SOILS USING THE
WHOLE-SOIL INOCULA TECHNIQUE 198 24. INVESTIGATING THE IMPORTANCE OF
OPTIMAL SOIL FERTILITY IN THE RESPONSE OF A LEGUME TO INOCULATION WITH
RHIZOBIA 206 ADDITIONAL REFERENCES AND RECOMMENDED READING 214 IV.
INOCULANT TECHNOLOGY 217 25. PRODUCING BROTH CULTURES IN SIMPLE GLASS
FERMENTORS 225 26. PRODUCING INOCULUM IN A STEEL FERMENTOR 232 27.
PREPARING A RANGE OF CARRIER MATERIALS AND PRODUCING INOCULANTS 240 28.
PREPARING INOCULANTS USING DILUTED CULTURES OF RHIZOBIA AND
PRESTERILIZED PEAT 249 29. TESTING THE SURVIVAL OF RHIZOBIA ON
INOCULATED SEEDS 259 ADDITIONAL REFERENCES AND RECOMMENDED READING 265
CONTENTS XV V. GENETIC TECHNIQUES FOR RHIZOBIA 267 30. ANALYZING PLASMID
PROFILES OF RHIZOBIUM SPP. BY A MODIFIED ECKHARDT VERTICAL GEL
ELECTROPHORESIS PROCEDURE 273 31. ISOLATING AND PURIFYING GENOMIC DNA OF
RHIZOBIA USING A LARGE-SCALE METHOD 279 32. ISOLATING AND PURIFYING
GENOMIC DNA OF RHIZOBIA USING A RAPID SMALL-SCALE METHOD 284 33.
DIGESTING GENOMIC DNA OF RHIZOBIA WITH RESTRICTION ENDONUCLEASES 289 34.
SEPARATING RESTRICTION FRAGMENTS OF GENOMIC DNA BY HORIZONTAL AGAROSE
GEL ELECTROPHORESIS 293 35. TRANSFERRING ELECTROPHORETICALLY SEPARATED
DNA FROM AGAROSE GELS TO A MEMBRANE BY SOUTHERN BLOTTING 298 36.
PREPARING A DNA PROBE FOR DETECTING THE NIF GENES ON SYMBIOTIC PLASMIDS
OF RHIZOBIUM SPP 303 37. INCORPORATING A NONRADIOACTIVE LABEL INTO A DNA
PROBE BY NICK TRANSLATION 310 38. USING A NONRADIOACTIVELY LABELED
NI/KDH GENE PROBE TO LOCATE COMPLEMENTARY SEQUENCES OF RHIZOBIAL DNA
IMMOBILIZED ON MEMBRANES 313 ADDITIONAL REFERENCES AND RECOMMENDED
READING 318 VI. APPENDICES 321 1. CHARACTERISTICS OF THE SUBFAMILIES OF
LEGUMES 323 2. THE NODULE PRESERVATION VIAL 332 3. BACTERIAL GROWTH
MEDIA AND PLANT NUTRIENT SOLUTIONS 333 4. REAGENTS AND BUFFERS 342 5.
MOLECULAR BIOLOGY REAGENTS AND BUFFERS 348 6. MCFARLAND NEPHELOMETER
BARIUM-SULFATE STANDARDS 356 7. PREPARING SEEDLING-AGAR SLANTS AND
NIFTAL-TUBES FOR CULTURING SMALL-SEEDED LEGUMES ; 358 8. BUILDING A RACK
FOR GROWTH POUCHES 362 9. RECOMMENDATIONS OF LEGUMES AND GROWTH SYSTEMS
FOR AUTHENTICATION 363 10. SEED SURFACE STERILIZATION AND GERMINATION
366 11. PREPARING LEONARD JARS 370 12. INJECTING AND BLEEDING RABBITS
372 13. THE INDIRECT FLUORESCENT ANTIBODY TECHNIQUE 377 14. ADDITIONAL
INFORMATION ON THE PLANT INFECTION COUNT 380 XVI CONTENTS 15. THE
ACETYLENE REDUCTION ASSAY FOR MEASURING NITROGENASE ACTIVITY 392 16.
METHODS FOR DETERMINING LIME REQUIREMENTS OF ACID SOILS 399 17. ANALYSIS
OF VARIANCE FOR A RHIZOBIAL STRAIN SELECTION EXPERIMENT 402 18.
COMPUTING THE COEFFICIENT OF CORRELATION (R) TO SHOW THE RELATIONSHIP
BETWEEN SHOOT AND NODULE WEIGHTS IN A RHIZOBIAL STRAIN SELECTION
EXPERIMENT 409 19. REPLICATORS AND MICROTITER PLATES 413 20. SEED
INOCULATION PROCEDURES 415 21. DETERMINING FIELD CAPACITY OF FIELD SOIL
421 22. THE SIMPLE TRANSFER CHAMBER 424 23. FREEZE DRYING CULTURES OF
RHIZOBIA 428 24. SOURCE OF RHIZOBIA 435 25. ABSORPTION OF ANTISERA 438
SUPPLEMENTAL READING LIST 439 INDEX 443
|
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| author | Somasegaran, Padmanabhan Hoben, Heinz J. |
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| classification_tum | BIO 310f BIO 284f |
| ctrlnum | (OCoLC)246824677 (DE-599)BVBBV009759769 |
| discipline | Biologie |
| format | Book |
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| indexdate | 2024-07-09T17:40:24Z |
| institution | BVB |
| isbn | 3540941347 0387941347 |
| language | English |
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| physical | XVI, 450 S. Ill., graph. Darst. |
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| spelling | Somasegaran, Padmanabhan Verfasser aut Handbook for rhizobia methods in legume rhizobium technology P. Somasegaran ; H. J. Hoben New York u.a. Springer 1994 XVI, 450 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Springer laboratory Literaturverz. S. 439 - 441 Stickstofffixierung (DE-588)4183273-5 gnd rswk-swf Symbiose (DE-588)4136029-1 gnd rswk-swf Rhizobium (DE-588)4178027-9 gnd rswk-swf Hülsenfrüchtler (DE-588)4026066-5 gnd rswk-swf Stickstofffixierung (DE-588)4183273-5 s Rhizobium (DE-588)4178027-9 s DE-604 Hülsenfrüchtler (DE-588)4026066-5 s Symbiose (DE-588)4136029-1 s Hoben, Heinz J. Verfasser aut GBV Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=006455955&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Somasegaran, Padmanabhan Hoben, Heinz J. Handbook for rhizobia methods in legume rhizobium technology Stickstofffixierung (DE-588)4183273-5 gnd Symbiose (DE-588)4136029-1 gnd Rhizobium (DE-588)4178027-9 gnd Hülsenfrüchtler (DE-588)4026066-5 gnd |
| subject_GND | (DE-588)4183273-5 (DE-588)4136029-1 (DE-588)4178027-9 (DE-588)4026066-5 |
| title | Handbook for rhizobia methods in legume rhizobium technology |
| title_auth | Handbook for rhizobia methods in legume rhizobium technology |
| title_exact_search | Handbook for rhizobia methods in legume rhizobium technology |
| title_full | Handbook for rhizobia methods in legume rhizobium technology P. Somasegaran ; H. J. Hoben |
| title_fullStr | Handbook for rhizobia methods in legume rhizobium technology P. Somasegaran ; H. J. Hoben |
| title_full_unstemmed | Handbook for rhizobia methods in legume rhizobium technology P. Somasegaran ; H. J. Hoben |
| title_short | Handbook for rhizobia |
| title_sort | handbook for rhizobia methods in legume rhizobium technology |
| title_sub | methods in legume rhizobium technology |
| topic | Stickstofffixierung (DE-588)4183273-5 gnd Symbiose (DE-588)4136029-1 gnd Rhizobium (DE-588)4178027-9 gnd Hülsenfrüchtler (DE-588)4026066-5 gnd |
| topic_facet | Stickstofffixierung Symbiose Rhizobium Hülsenfrüchtler |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=006455955&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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