Änderungen der extracellulären Matrix und des MMP-1, MMP-13 in der Wundheilung der Narbenhernien und Redizivnarbenhernien: = Changes of the extracellular matrix and MMP-1, MMP-13 in wound healing of incisional hernia and recurrent incisional hernia
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
1999
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | Aachen, Techn. Hochsch., Diss., 1999 |
| Beschreibung: | II, 83 S. Ill., graph. Darst. 21 cm |
Internformat
MARC
| LEADER | 00000nam a22000001c 4500 | ||
|---|---|---|---|
| 001 | BV013064091 | ||
| 003 | DE-604 | ||
| 007 | t | ||
| 008 | 000229s1999 gw ad|| m||| 00||| eng d | ||
| 016 | 7 | |a 958256918 |2 DE-101 | |
| 035 | |a (OCoLC)247207147 | ||
| 035 | |a (DE-599)BVBBV013064091 | ||
| 040 | |a DE-604 |b ger |e rakddb | ||
| 041 | 0 | |a eng | |
| 044 | |a gw |c DE | ||
| 049 | |a DE-355 | ||
| 084 | |a 610 |2 sdnb | ||
| 100 | 1 | |a Si, Zhongyi |d 1964- |e Verfasser |0 (DE-588)121705374 |4 aut | |
| 245 | 1 | 0 | |a Änderungen der extracellulären Matrix und des MMP-1, MMP-13 in der Wundheilung der Narbenhernien und Redizivnarbenhernien |b = Changes of the extracellular matrix and MMP-1, MMP-13 in wound healing of incisional hernia and recurrent incisional hernia |c vorgelegt von Zhongyi Si |
| 246 | 1 | 1 | |a Changes of the extracellular matrix and MMP-1, MMP-13 in wound healing of incisional hernia and recurrent incisional hernia |
| 264 | 1 | |c 1999 | |
| 300 | |a II, 83 S. |b Ill., graph. Darst. |c 21 cm | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 500 | |a Aachen, Techn. Hochsch., Diss., 1999 | ||
| 650 | 4 | |a Hochschulschrift | |
| 655 | 7 | |0 (DE-588)4113937-9 |a Hochschulschrift |2 gnd-content | |
| 856 | 4 | 2 | |m DNB Datenaustausch |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=008901083&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
| 943 | 1 | |a oai:aleph.bib-bvb.de:BVB01-008901083 | |
Datensatz im Suchindex
| _version_ | 1807681506055815168 |
|---|---|
| adam_text |
CONTENTS
_
_
CONTENTS
1.
INTRODUCTION
1
1.2.
SCAR,
KELOID
AND
CICATRIX
2
1.3.
EXTRACELLULAR
MATRIX
AND
WOUND
HEALING
3
1.4.
DISEASES
OF
THE
EXTRACELLULAR
MATRIX
4
1.5.
COLLAGEN
4
1.5.1.
COLLAGEN
STRUCTURE
4
1.5.2.
COLLAGEN
MORPHOLOGY
6
1.5.3.
FIBRE
STRUCTURE
6
1.5.4.
COLLAGEN
BIOSYNTHESIS
7
1.5.5.
COLLAGEN
DEGRADATION
8
1.5.6.
DISEASES
OF
COLLAGEN
9
1.6.
FIBRONECTIN
10
1.7.
TENASCIN
12
1.8.
MATRIX
METALLOPROTEINASES
13
2.
AIMS
OF
THIS
WORK
15
3.
MATERIALS
AND
METHODS
16
3.1.
MATERIALS
16
3.1.1.
INSTRUMENTS
16
3.1.2.
CHEMICAL
REAGENTS
16
3.1.3.
BIOLOGICAL
SAMPLES
17
3.2.
METHODS
18
3.2.1.TISSUE
PROCESSING
18
3.2.2.
PARAFFIN
SECTION
19
3.2.3.
FROZEN
SECTION
19
3.2.4.
HAEMATOXYLIN
AND
EOSIN
STAINING
20
3.2.5.
IMMUNOHISTOCHEMISTRY
20
3.2.5.1.
DEFINITION
AND
PRINCIPLE
20
3.2.5.2.
IMMUNOPEROXIDASE
STAINING
21
3.2.5.3.
PARAFFIN
SECTIONS
AND
INDIRECT
IMMUNOPEROXIDASE
STAINING
FOR
THE
DETECTION
OF
MMP-1
AND
MMP-13
22
3.2.5.4.
FROZEN
SECTIONS
AND
IMMUNOPEROXIDASE
STAINING
FOR
THE
DETECTION
OF
COLLAGEN
I,
III.
FIBRONECTIN
AND
TENASCIN
23
3.2.6.
COLLAGEN
EXTRACTION
24
3.2.7.
DIALYSIS
25
3.2.8.
LYOPHILIZATION
26
3.2.9.
TESTING
FOR
ANTIBODIES
RECOGNIZING
THE
COLLAGEN
26
3.2.10.
ELECTROPHORESIS
27
3
2.10.1
DISCONTINUOUS
BUFFER
SYSTEM
27
CONTENTS
3.2.10
2.
ELECTROPHORESIS
PROCEDURE
28
3.2.11.
WESTERN
BLOTTING
29
3.2.11.1.
TRANSFER
BUFFER
29
3.2.11.2.
TRANSFER
PROCEDURE
29
3.2.11.3.
IMMUNOLOGICAL
DETECTION
30
3.3.
STATISTICAL
ANALYSIS
31
4.
RESULTS
32
4.1.
IMMUNOHISTOCHEMICAL
RESULTS
32
4.1.1.
EXPRESSION
OF
TENASCIN
IN
IMMUNOHISTOCHEMICAL
STAINED
TISSUES
32
4.1.1.1
TN
EXPRESSION
IN
SKIN
32
4.1.
1.2.TN
EXPRESSION
IN
FASCIA
33
4.1.2.
EXPRESSION
OF
FIBRONECTIN
IN
IMMUNOHISTOCHEMICAL
STAINED
TISSUES
35
4.1.21.
FN
EXPRESSION
IN
SKIN
35
4.2.1.2.
EXPRESSION
OF
FN
IN
FASCIA
37
4.1.3.
EXPRESSION
OF
MMP-1
IN
IMMUNOHISTOCHEMICAL
STAINED
TISSUES
38
4.1.3.1.
EXPRESSION
OF
MMP-1
IN
SKIN
38
4.1.3.2.
EXPRESSION
OF
MMP-1
IN
FASCIA
40
4.1.4.
EXPRESSION
OF
MMP-13
IN
IMMUNOHISTOCHEMICAL
STAINED
TISSUES.
41
4.2.
RATIO
OF
COLLAGEN
I
TO
COLLAGEN
HI
43
4.2.1.
MORPHOMETRIC
RATIO
OF
COLLAGEN
I
TO
COLLAGEN
M
43
4.2.1.1.
MORPHOMETRIC
RATIO
OF
COLLAGEN
I
TO
COLLAGEN
III
IN
SKIN
43
4.2.1
2.
MORPHOMETRIC
RATIO
OF
COLLAGEN
I
TO
COLLAGEN
III
IN
FASCIA
44
4.2.
OBSERVATIONS
OF
WESTERN
BLOTTING
RESULTS
AND
ANALYSIS
OF
THE
RATIO
OF
COLLAGEN
I
/
M
46
4.3.
CORRELATION
OF
IMMUNOHISTOCHEMISTRY
AND
WESTERN
BLOTTING
49
5.
DISCUSSION
51
6.
SUMMARY
65
7.
ZUSAMMENFASSUNG
68
8.
REFERENCES:
71
9.
APPENDIX
80
9.1.
ABBREVIATIONS
80
9.2.
ACKNOWLEDGEMENTS
81
9.3.
CURRICULUM
VITAE
82 |
| any_adam_object | 1 |
| author | Si, Zhongyi 1964- |
| author_GND | (DE-588)121705374 |
| author_facet | Si, Zhongyi 1964- |
| author_role | aut |
| author_sort | Si, Zhongyi 1964- |
| author_variant | z s zs |
| building | Verbundindex |
| bvnumber | BV013064091 |
| ctrlnum | (OCoLC)247207147 (DE-599)BVBBV013064091 |
| discipline | Medizin |
| format | Book |
| fullrecord | <?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>00000nam a22000001c 4500</leader><controlfield tag="001">BV013064091</controlfield><controlfield tag="003">DE-604</controlfield><controlfield tag="007">t</controlfield><controlfield tag="008">000229s1999 gw ad|| m||| 00||| eng d</controlfield><datafield tag="016" ind1="7" ind2=" "><subfield code="a">958256918</subfield><subfield code="2">DE-101</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(OCoLC)247207147</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)BVBBV013064091</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-604</subfield><subfield code="b">ger</subfield><subfield code="e">rakddb</subfield></datafield><datafield tag="041" ind1="0" ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="044" ind1=" " ind2=" "><subfield code="a">gw</subfield><subfield code="c">DE</subfield></datafield><datafield tag="049" ind1=" " ind2=" "><subfield code="a">DE-355</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">610</subfield><subfield code="2">sdnb</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Si, Zhongyi</subfield><subfield code="d">1964-</subfield><subfield code="e">Verfasser</subfield><subfield code="0">(DE-588)121705374</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Änderungen der extracellulären Matrix und des MMP-1, MMP-13 in der Wundheilung der Narbenhernien und Redizivnarbenhernien</subfield><subfield code="b">= Changes of the extracellular matrix and MMP-1, MMP-13 in wound healing of incisional hernia and recurrent incisional hernia</subfield><subfield code="c">vorgelegt von Zhongyi Si</subfield></datafield><datafield tag="246" ind1="1" ind2="1"><subfield code="a">Changes of the extracellular matrix and MMP-1, MMP-13 in wound healing of incisional hernia and recurrent incisional hernia</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">1999</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">II, 83 S.</subfield><subfield code="b">Ill., graph. Darst.</subfield><subfield code="c">21 cm</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="b">n</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="b">nc</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="500" ind1=" " ind2=" "><subfield code="a">Aachen, Techn. Hochsch., Diss., 1999</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Hochschulschrift</subfield></datafield><datafield tag="655" ind1=" " ind2="7"><subfield code="0">(DE-588)4113937-9</subfield><subfield code="a">Hochschulschrift</subfield><subfield code="2">gnd-content</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="m">DNB Datenaustausch</subfield><subfield code="q">application/pdf</subfield><subfield code="u">http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=008901083&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA</subfield><subfield code="3">Inhaltsverzeichnis</subfield></datafield><datafield tag="943" ind1="1" ind2=" "><subfield code="a">oai:aleph.bib-bvb.de:BVB01-008901083</subfield></datafield></record></collection> |
| genre | (DE-588)4113937-9 Hochschulschrift gnd-content |
| genre_facet | Hochschulschrift |
| id | DE-604.BV013064091 |
| illustrated | Illustrated |
| indexdate | 2024-08-18T00:03:43Z |
| institution | BVB |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-008901083 |
| oclc_num | 247207147 |
| open_access_boolean | |
| owner | DE-355 DE-BY-UBR |
| owner_facet | DE-355 DE-BY-UBR |
| physical | II, 83 S. Ill., graph. Darst. 21 cm |
| publishDate | 1999 |
| publishDateSearch | 1999 |
| publishDateSort | 1999 |
| record_format | marc |
| spelling | Si, Zhongyi 1964- Verfasser (DE-588)121705374 aut Änderungen der extracellulären Matrix und des MMP-1, MMP-13 in der Wundheilung der Narbenhernien und Redizivnarbenhernien = Changes of the extracellular matrix and MMP-1, MMP-13 in wound healing of incisional hernia and recurrent incisional hernia vorgelegt von Zhongyi Si Changes of the extracellular matrix and MMP-1, MMP-13 in wound healing of incisional hernia and recurrent incisional hernia 1999 II, 83 S. Ill., graph. Darst. 21 cm txt rdacontent n rdamedia nc rdacarrier Aachen, Techn. Hochsch., Diss., 1999 Hochschulschrift (DE-588)4113937-9 Hochschulschrift gnd-content DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=008901083&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Si, Zhongyi 1964- Änderungen der extracellulären Matrix und des MMP-1, MMP-13 in der Wundheilung der Narbenhernien und Redizivnarbenhernien = Changes of the extracellular matrix and MMP-1, MMP-13 in wound healing of incisional hernia and recurrent incisional hernia Hochschulschrift |
| subject_GND | (DE-588)4113937-9 |
| title | Änderungen der extracellulären Matrix und des MMP-1, MMP-13 in der Wundheilung der Narbenhernien und Redizivnarbenhernien = Changes of the extracellular matrix and MMP-1, MMP-13 in wound healing of incisional hernia and recurrent incisional hernia |
| title_alt | Changes of the extracellular matrix and MMP-1, MMP-13 in wound healing of incisional hernia and recurrent incisional hernia |
| title_auth | Änderungen der extracellulären Matrix und des MMP-1, MMP-13 in der Wundheilung der Narbenhernien und Redizivnarbenhernien = Changes of the extracellular matrix and MMP-1, MMP-13 in wound healing of incisional hernia and recurrent incisional hernia |
| title_exact_search | Änderungen der extracellulären Matrix und des MMP-1, MMP-13 in der Wundheilung der Narbenhernien und Redizivnarbenhernien = Changes of the extracellular matrix and MMP-1, MMP-13 in wound healing of incisional hernia and recurrent incisional hernia |
| title_full | Änderungen der extracellulären Matrix und des MMP-1, MMP-13 in der Wundheilung der Narbenhernien und Redizivnarbenhernien = Changes of the extracellular matrix and MMP-1, MMP-13 in wound healing of incisional hernia and recurrent incisional hernia vorgelegt von Zhongyi Si |
| title_fullStr | Änderungen der extracellulären Matrix und des MMP-1, MMP-13 in der Wundheilung der Narbenhernien und Redizivnarbenhernien = Changes of the extracellular matrix and MMP-1, MMP-13 in wound healing of incisional hernia and recurrent incisional hernia vorgelegt von Zhongyi Si |
| title_full_unstemmed | Änderungen der extracellulären Matrix und des MMP-1, MMP-13 in der Wundheilung der Narbenhernien und Redizivnarbenhernien = Changes of the extracellular matrix and MMP-1, MMP-13 in wound healing of incisional hernia and recurrent incisional hernia vorgelegt von Zhongyi Si |
| title_short | Änderungen der extracellulären Matrix und des MMP-1, MMP-13 in der Wundheilung der Narbenhernien und Redizivnarbenhernien |
| title_sort | anderungen der extracellularen matrix und des mmp 1 mmp 13 in der wundheilung der narbenhernien und redizivnarbenhernien changes of the extracellular matrix and mmp 1 mmp 13 in wound healing of incisional hernia and recurrent incisional hernia |
| title_sub | = Changes of the extracellular matrix and MMP-1, MMP-13 in wound healing of incisional hernia and recurrent incisional hernia |
| topic | Hochschulschrift |
| topic_facet | Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=008901083&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT sizhongyi anderungenderextracellularenmatrixunddesmmp1mmp13inderwundheilungdernarbenhernienundredizivnarbenhernienchangesoftheextracellularmatrixandmmp1mmp13inwoundhealingofincisionalherniaandrecurrentincisionalhernia AT sizhongyi changesoftheextracellularmatrixandmmp1mmp13inwoundhealingofincisionalherniaandrecurrentincisionalhernia |