The role of flavonols and anthocyanins in the cold and UV-B acclimation of Arabidopsis thaliana (L.):
Gespeichert in:
| 1. Verfasser: | |
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| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
2014
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| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | IX, 159 S. Ill., graph. Darst. |
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| 245 | 1 | 0 | |a The role of flavonols and anthocyanins in the cold and UV-B acclimation of Arabidopsis thaliana (L.) |c von Elisa Schulz |
| 264 | 1 | |c 2014 | |
| 300 | |a IX, 159 S. |b Ill., graph. Darst. | ||
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Datensatz im Suchindex
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| adam_text | TABLE OF CONTENTS III
TABLE OF CONTENTS
ZUSAMMENFASSUNG I
SUMMARY II
TABLE OF CONTENTS ILL
LIST OF FIGURES VI
LIST OF TABLES VIII
LIST OF ABBREVIATIONS IX
1 INTRODUCTION 1
1.1 PLANTS UNDER COLD AND UV-B LIGHT STRESS 1
1.1.1 COLD TEMPERATURE, FREEZING STRESS AND DAMAGE 3
1.1.2 COLD ACCLIMATION AND FREEZING TOLERANCE 3
1.1.3 UV-B LIGHT STRESS AND DAMAGE 6
1.1.4 UV-B LIGHT ACCLIMATION AND TOLERANCE 7
1.1.5 IMPACT OF COMBINED STRESS ON PLANT PERFORMANCE 10
1.2 THE ROLE OF FLAVONOIDS IN PLANT STRESS TOLERANCE 11
1.2.1 FLAVONOID BIOSYNTHETIC PATHWAY IN ARABIDOPSIS AND MUTANTS CHANGED
IN
FLAVONOID BIOSYNTHESIS 13
1.2.2 ROLE OF FLAVONOIDS IN COLD ACCLIMATION AND FREEZING TOLERANCE 17
1.2.3 ROLE OF FLAVONOIDS IN UV-B LIGHT TOLERANCE 18
1.3 AIMS OF THE THESIS 19
2 MATERIAL AND METHODS 20
2.1 EQUIPMENT 20
2.2 CONSUMABLES 21
2.3 REAGENTS, KITS, ENZYMES AND CHEMICALS 21
2.4 OLIGONUCLEOTIDES 21
2.5 SOFTWARE 23
2.6 PLANT MATERIAL AND PLANT CULTIVATION 23
2.6.1 PLANT MATERIAL 23
2.6.2 PLANT CULTIVATION AND HARVEST 25
2.7 PHYSIOLOGICAL METHODS 27
2.7.1 FREEZING OF DETACHED LEAVES 27
2.7.2 DETERMINATION OF FREEZING TOLERANCE OF DETACHED LEAVES VIA
ELECTROLYTE
LEAKAGE 27
2.7.3 CHLOROPHYLL FLUORESCENCE IMAGING 27
2.8 BIOCHEMICAL METHODS 28
HTTP://D-NB.INFO/1070689734
IV
TABLE OF CONTENTS
2.8.1 SECONDARY METABOLITE PROFILING BY LC-MS 28
2.8.2 PRIMARY METABOLITE PROFILING BY GC-MS 29
2.8.3 COMBINED METABOLITE PROFILING BY GC-MS AND LC-MS 30
2.8.4 DETERMINATION OF SUGAR CONTENT BY HPAEC-PAD 31
2.9 MOLECULAR BIOLOGICAL METHODS 32
2.9.1 QUANTITATIVE RT-PCR (QRT-PCR) 32
2.10 STATISTICS 33
3 RESULTS 34
3.1 INVESTIGATION OF THE IMPORTANCE OF FLAVONOIDS FOR COLD ACCLIMATION
IN
NATURAL ACCESSIONS OF ARABIDOPSIS 34
3.1.1 EXPRESSION OF FLAVONOID BIOSYNTHESIS GENES IN 53 ARABIDOPSIS
ACCESSIONS 34
3.1.2 FLAVONOID PATTERNS IN 54 ARABIDOPSIS ACCESSIONS 37
3.1.3 STRUCTURING OF TRANSCRIPT AND METABOLITE DATASETS BY PRINCIPAL
COMPONENT
ANALYSIS AND STANDARD BOXPLOTS 39
3.1.4 CORRELATIONS BETWEEN FLAVONOID TRANSCRIPT AND METABOLITE DATA 43
3.1.5 CORRELATIONS BETWEEN TRANSCRIPT OR FLAVONOID LEVELS AND FREEZING
TOLERANCE
IN ARABIDOPSIS ACCESSIONS 46
3.2 ANALYSIS OF THE ROLE OF FLAVONOIDS IN FREEZING TOLERANCE OF
ARABIDOPSIS
MUTANT LINES CHANGED IN FLAVONOID BIOSYNTHESIS 48
3.2.1 FREEZING TOLERANCE OF MUTANT LINES WITH CHANGES IN FLAVONOID
BIOSYNTHESIS 50
3.2.2 EXPRESSION OF FLAVONOID BIOSYNTHESIS GENES IN MUTANT LINES CHANGED
IN
FLAVONOID BIOSYNTHESIS 56
3.2.3 SECONDARY METABOLITE PROFILING FOR DETERMINATION OF THE CONTENT OF
SPECIFIC
FLAVONOIDS IN MUTANT LINES CHANGED IN FLAVONOID BIOSYNTHESIS 61
3.2.4 PRIMARY METABOLISM IS NOT SIGNIFICANTLY AFFECTED BY MUTATIONS IN
FLAVONOID
BIOSYNTHESIS 67
3.3 INVESTIGATION OF TRANSCRIPTIONAL AND METABOLIC RESPONSES OF 52
NATURAL
ACCESSIONS TO LOW TEMPERATURE AND UV-B 69
3.3.1 EXPRESSION OF STRESS RESPONSIVE AND FLAVONOID BIOSYNTHESIS GENES
70
3.3.2 PROFILING OF FLAVONOID CONTENT 76
3.3.3 CORRELATIONS BETWEEN TRANSCRIPT AND FLAVONOID LEVELS 80
3.3.4 CORRELATIONS BETWEEN TRANSCRIPT OR FLAVONOID LEVELS AND LEAF
FREEZING
TOLERANCE 83
3.3.5 PROFILING OF PRIMARY METABOLITES 85
4 DISCUSSION 92
4.1 IMPORTANCE OF FLAVONOIDS FOR COLD ACCLIMATION AND FREEZING TOLERANCE
IN
NATURAL ACCESSIONS OF ARABIDOPSIS 92
4.2 ANALYSIS OF MUTANTS CHANGED IN FLAVONOID BIOSYNTHESIS INDICATES
DIFFERENT
POTENTIAL ROLES OF FLAVONOIDS IN FREEZING TOLERANCE 98
TABLE OF CONTENTS V
4.3 TRANSCRIPTIONAL AND METABOLIC RESPONSES OF 52 NATURAL ACCESSIONS TO
LOW
TEMPERATURE AND TJV-B 105
4.4 IDENTIFICATION OF SPECIFIC FLAVONOLS AND ANTHOCYANINS AS MAIN
PLAYERS IN
THE COLD AND UV-B RESPONSE OF ARABIDOPSIS 114
5 OUTLOOK 118
6 REFERENCES 119
7 SUPPLEMENTAL 137
ACKNOWLEDGEMENTS 156
LIST OF PUBLICATIONS AND PRESENTATIONS 157
EIDESSTATTLICHE ERKLARUNG 159
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| dewey-search | 571.934642364 |
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| dewey-tens | 570 - Biology |
| discipline | Biologie |
| format | Thesis Book |
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| spelling | Schulz, Elisa Verfasser (DE-588)106884180X aut The role of flavonols and anthocyanins in the cold and UV-B acclimation of Arabidopsis thaliana (L.) von Elisa Schulz 2014 IX, 159 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Potsdam, Univ., Diss., 2014 Ackerschmalwand (DE-588)4141299-0 gnd rswk-swf Flavonol (DE-588)4154586-2 gnd rswk-swf Ultraviolett B (DE-588)4186734-8 gnd rswk-swf Anthocyane (DE-588)4142621-6 gnd rswk-swf Kälte (DE-588)4162981-4 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Ackerschmalwand (DE-588)4141299-0 s Ultraviolett B (DE-588)4186734-8 s Kälte (DE-588)4162981-4 s Flavonol (DE-588)4154586-2 s Anthocyane (DE-588)4142621-6 s DE-188 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=028012946&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Schulz, Elisa The role of flavonols and anthocyanins in the cold and UV-B acclimation of Arabidopsis thaliana (L.) Ackerschmalwand (DE-588)4141299-0 gnd Flavonol (DE-588)4154586-2 gnd Ultraviolett B (DE-588)4186734-8 gnd Anthocyane (DE-588)4142621-6 gnd Kälte (DE-588)4162981-4 gnd |
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| title | The role of flavonols and anthocyanins in the cold and UV-B acclimation of Arabidopsis thaliana (L.) |
| title_auth | The role of flavonols and anthocyanins in the cold and UV-B acclimation of Arabidopsis thaliana (L.) |
| title_exact_search | The role of flavonols and anthocyanins in the cold and UV-B acclimation of Arabidopsis thaliana (L.) |
| title_full | The role of flavonols and anthocyanins in the cold and UV-B acclimation of Arabidopsis thaliana (L.) von Elisa Schulz |
| title_fullStr | The role of flavonols and anthocyanins in the cold and UV-B acclimation of Arabidopsis thaliana (L.) von Elisa Schulz |
| title_full_unstemmed | The role of flavonols and anthocyanins in the cold and UV-B acclimation of Arabidopsis thaliana (L.) von Elisa Schulz |
| title_short | The role of flavonols and anthocyanins in the cold and UV-B acclimation of Arabidopsis thaliana (L.) |
| title_sort | the role of flavonols and anthocyanins in the cold and uv b acclimation of arabidopsis thaliana l |
| topic | Ackerschmalwand (DE-588)4141299-0 gnd Flavonol (DE-588)4154586-2 gnd Ultraviolett B (DE-588)4186734-8 gnd Anthocyane (DE-588)4142621-6 gnd Kälte (DE-588)4162981-4 gnd |
| topic_facet | Ackerschmalwand Flavonol Ultraviolett B Anthocyane Kälte Hochschulschrift |
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