Role of Peroxiredoxin 6 in human melanoma: = Die Funktion von Peroxiredoxin 6 im humanen Melanom
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
Würzburg
2015
|
| Schlagworte: | |
| Online-Zugang: | kostenfrei Inhaltsverzeichnis |
| Beschreibung: | 94 Blatt Illustrationen, Diagramme |
Internformat
MARC
| LEADER | 00000nam a2200000 c 4500 | ||
|---|---|---|---|
| 001 | BV043527707 | ||
| 003 | DE-604 | ||
| 005 | 20160427 | ||
| 007 | t | ||
| 008 | 160426s2015 a||| m||| 00||| eng d | ||
| 035 | |a (OCoLC)951550396 | ||
| 035 | |a (DE-599)BVBBV043527707 | ||
| 040 | |a DE-604 |b ger |e rda | ||
| 041 | 0 | |a eng | |
| 049 | |a DE-384 |a DE-473 |a DE-703 |a DE-1051 |a DE-824 |a DE-29 |a DE-12 |a DE-91 |a DE-19 |a DE-1049 |a DE-92 |a DE-739 |a DE-898 |a DE-355 |a DE-706 |a DE-20 |a DE-1102 | ||
| 100 | 1 | |a Schmitt, Alexandra |d ca. 21. Jh |e Verfasser |0 (DE-588)1098195515 |4 aut | |
| 245 | 1 | 0 | |a Role of Peroxiredoxin 6 in human melanoma |b = Die Funktion von Peroxiredoxin 6 im humanen Melanom |c vorgelegt von Alexandra Schmitt aus Würzburg |
| 246 | 1 | 1 | |a Die Funktion von Peroxiredoxin 6 im humanen Melanom |
| 264 | 1 | |a Würzburg |c 2015 | |
| 300 | |a 94 Blatt |b Illustrationen, Diagramme | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 502 | |b Dissertation |c Bayerische Julius-Maximilians-Universität Würzburg |d 2015 | ||
| 546 | |a Zusammenfassung in deutscher und englischer Sprache | ||
| 650 | 0 | 7 | |a Prostaglandin E2 |0 (DE-588)4175971-0 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Peroxiredoxin |0 (DE-588)1028994311 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Arachidonsäure |0 (DE-588)4142846-8 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Melanom |0 (DE-588)4074707-4 |2 gnd |9 rswk-swf |
| 655 | 7 | |0 (DE-588)4113937-9 |a Hochschulschrift |2 gnd-content | |
| 689 | 0 | 0 | |a Peroxiredoxin |0 (DE-588)1028994311 |D s |
| 689 | 0 | 1 | |a Melanom |0 (DE-588)4074707-4 |D s |
| 689 | 0 | 2 | |a Arachidonsäure |0 (DE-588)4142846-8 |D s |
| 689 | 0 | 3 | |a Prostaglandin E2 |0 (DE-588)4175971-0 |D s |
| 689 | 0 | |5 DE-604 | |
| 776 | 0 | 8 | |i Erscheint auch als |n Online-Ausgabe |o urn:nbn:de:bvb:20-opus-111465 |
| 856 | 4 | 1 | |u https://nbn-resolving.org/urn:nbn:de:bvb:20-opus-111465 |x Resolving-System |z kostenfrei |3 Volltext |
| 856 | 4 | 2 | |m DNB Datenaustausch |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=028943483&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
| 912 | |a ebook | ||
| 999 | |a oai:aleph.bib-bvb.de:BVB01-028943483 | ||
Datensatz im Suchindex
| _version_ | 1804176177523326976 |
|---|---|
| adam_text | ROLE OF PEROXIREDOXIN 6 IN HUMAN MELANOMA
DISSERTATION ZUR ERLANGUNG DES NATURWISSENSCHAFTLICHEN DOKTORGRADES
DER BAYERISCHEN JULIUS-MAXIMHIANS-UNIVERSITAET WUERZBURG
VORGELEGT VON
ALEXANDRA SCHMITT
AUS WUERZBURG
WUERZBURG 2015
TABLE OF CONTENTS
1.
ABSTRACT....................................................................................................................1
2*
ZUSAMMENFASSUNG.................................................................................................
3
3* INTRODUCTION...* 5
3.1 M ELANOM
A....................................................................................................................
5
3.1.1 COMMON MELANOMA SIGNALING
PATHWAYS.................................................................
7
3.1.2 OXIDATIVE STRESS IN
MELANOMA.................................................................................10
3.2 THE XMRK AND HERMRK MELANOMA MODELS
.........................................................
11
3.3 PEROXIREDOXIN 6
..........................................................................................................12
3.3.1 PEROXIDASE ACTIVITY OF
PRDX6.................................................................................12
3.3.2 PIA
2
ACTIVITY OF
PRDX6..........................................................................................13
3.3.3 PRDX6 IN
CANCER....................................................................................................
15
3-4 AIM OF THE
THESIS.........................................................................................................16
4* MATERIAL AND METHODS
...........................................................................................
17
4.1 M
ATERIAL..........................................................................................................
17
4.1.1 CELL
LINES..................................................................................................................17
4.1.2
PLASMIDS.................................................................................................................
17
4.1.3 OLIGONUCLEOTIDES FOR CLONING AND
REAL-TIME-PCR.................................................... 18
4.1.4
SIRNAS...................................................................................................................
20
4.1.5
ANTIBODIES...............................................................................................................
20
4.1.6 INHIBITORS
*
DRUGS AND COMPOUNDS
...........................................................................
21
4.1.7 ENZYMES
4.1.8 TRANSFECTION
REAGENTS.............................................................................................23
4.1.9
KITS.........................................................................................................................
23
4.1.10 TECHNICAL
EQUIPMENT............................................................................................24
4.1.11 BUFFER AND
MEDIA..................................................................................................24
4.1.11.1 STANDARD
BUFFER..............................................................................................24
4.1.11.2 CELL CULTURE
MEDIA..........................................................................................25
4.1.11.3 CELL CULTURE
BUFFER...........................................................................................25
4.1.11.4 BACTERIAL CULTURE MEDIA
..................................................................................
26
4.2 M
ETHODS.....................................................................................................................
27
4.2.1 CELL CULTURE
METHODS.............................................................................................
27
4.2.1.1 MAINTENANCE OF CELL LINES
.................................................................................
27
4.2.1.2 CRYOPRESERVATION OF CELL
LINES..........................................................................
27
4.21.3 THAWING OF CELL LINES
........................................................................................
27
4.2.1 *4 LENTIVIRAL INFECTION AND ESTABLISHMENT OF STABLE TRANSGENIC
CELL L I N E S . . 27
4.2.1 *5 SIRNA
TRANSFECTION..........................................................................................28
4.2.1.6 PROLIFERATION AND CELL GROWTH
ASSAYS................................................................29
4-2.1.6.1 CELL GROWTH ASSAY PERFORMED BY MANUAL CELL COUNTING
.**********29
4.2.1.6.2 ANALYSIS OF BRDU
INCORPORATION..................................................................
29
4.2.1.6.3 XCELLIGENCE ASSAY
..................................................................................
29
4.2.1.7 LUCIFERASE
ASSAY..............................................................................................30
4.2.1.8
IMMUNOFLUORESCENCE........................................................................................30
4.2.1.9 GAS CHROMATOGRAPHY ANALYSIS
.........................................................................
31
4.2.1.10
PGE2-EUSA...................................................................................................32
4.2.2 PROTEIN
METHODS.....................................................................................................32
4.2.2.1 CELL LYSATE
PREPARATION.....................................................................................32
4.2.2.2 SDS-PAGE AND WESTERN BLOT
..........................................................................
32
4.2.2.3
CO-MMUNOPRECPTATON...................................................................................33
4.2.3 DNA
METHODS.........................................................................................................
33
4.2.3.1 RESTRICTION ENZYME DIGESTION AND
LIGATION.......................................................33
4.2.3.2 PLASMID CONSTRUCTION AND SITE-DIRECTED MUTAGENESIS
......................................
34
4.2.3.3 TRANSFORMATION
*
COLONY SCREEN AND PLASMID PREPARATION ................................36
4.2.4 RNA AND CDNA
METHODS........................................................................................37
4.2.4.1 RNA EXTRACTION AND CDNA
SYNTHESIS..............................................................37
4
*
2.4
*
2
REALTIME-PCR.................................................................................................37
4.2.43
MICROARRAY........................................................................................................
38
5.
RESULTS....................................................................................................................39
5.1 REGULATION OF
PRDX6................................................................................................39
5.1.1 PRDX6 S A DIRECT TARGET OF XMRK AND THE HUMAN
EGFR.......................................39
5.1.2 PRDX6 IS REGULATED BY THE **3* PATHWAY
.............................................................
42
5.1.3 REACTIVE OXYGEN SPECIES HAVE NO INFLUENCE ON PRDX6 LEVEL IN
MELANOMA CELLS
AND AFTER HERMRK
STIMULATION..........................................................................................43
5.2 LOCALISATION OF PRDX6 AND TS INTERACTION WITH PC
NA....*.**....**... 44
5.3 FUNCTION OF PRDX6 IN HUMAN MELANOMA
CELLS....................................................47
5.31 KNOCKDOWN OF PRDX6 REDUCES
PROLIFERATION..........................................................47
5.3.2 KNOCKDOWN OF PRDX6 DECREASES P-RB PROTEIN AND P-SRC FAMILY KINASE
LEVELS..51
5.3.3 PRDX6 INFLUENCES PROLIFERATION VIA ITS IPU 2 ACTIVITY
..............................................
52
5.3.4 KNOCKDOWN OF PRDX6 LOWERS THE AMOUNT OF ARACHIDONIC ACID AND
DECREASES THE
LEVEL OF PGE2IN UACC-62
CELLS.......................................................................................57
5.3.5 THE PROLIFERATIVE FUNCTION OF THE IPL
*
2
ACTIVITY OF PRDX6 S MEDIATED AA ..**59
6
DISCUSSION..............................................................................................******
62
6.1 PRDX6 IN OTHER TUMOR
ENTITIES................................................................................62
6.2 ANALYSIS OF PRDX6 LEVELS AND TS REGULATION IN MELANOCYTES AND
MELANOMA
CELLS
......................................
62
6.3 THE FUNCTION OF PRDX6 IN HUMAN MELANOMA CELLS
..............................................
64
6.4 PRDX6 - A TARGET FOR MELANOMA
THERAPY?............................................................. 67
6.5 CONCLUDING REM
ARKS..................................................................................................69
7.
APPENDIX................................................................................................................71
7.1 ROLE OF PRDX6 IN MANIPULATING MELANOMA RESISTANCE TO VEM
URAFENIB........... 71
7.2
MICROARRAY..................................................................................................................
72
8. B*BLIOGRAPHY-*-.-*
*
.-**
*
*
*
*-*M*.. . . . . . . . . . . . . . . 7 5
****
.
***
.
****
**********
.
**
.
*
******
.
*
..
*
..
.*******
********
9
*
ACKNOWLEDGEMENTS..............................................................................................
94
|
| any_adam_object | 1 |
| author | Schmitt, Alexandra ca. 21. Jh |
| author_GND | (DE-588)1098195515 |
| author_facet | Schmitt, Alexandra ca. 21. Jh |
| author_role | aut |
| author_sort | Schmitt, Alexandra ca. 21. Jh |
| author_variant | a s as |
| building | Verbundindex |
| bvnumber | BV043527707 |
| collection | ebook |
| ctrlnum | (OCoLC)951550396 (DE-599)BVBBV043527707 |
| format | Thesis Book |
| fullrecord | <?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>02155nam a2200445 c 4500</leader><controlfield tag="001">BV043527707</controlfield><controlfield tag="003">DE-604</controlfield><controlfield tag="005">20160427 </controlfield><controlfield tag="007">t</controlfield><controlfield tag="008">160426s2015 a||| m||| 00||| eng d</controlfield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(OCoLC)951550396</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)BVBBV043527707</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-604</subfield><subfield code="b">ger</subfield><subfield code="e">rda</subfield></datafield><datafield tag="041" ind1="0" ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="049" ind1=" " ind2=" "><subfield code="a">DE-384</subfield><subfield code="a">DE-473</subfield><subfield code="a">DE-703</subfield><subfield code="a">DE-1051</subfield><subfield code="a">DE-824</subfield><subfield code="a">DE-29</subfield><subfield code="a">DE-12</subfield><subfield code="a">DE-91</subfield><subfield code="a">DE-19</subfield><subfield code="a">DE-1049</subfield><subfield code="a">DE-92</subfield><subfield code="a">DE-739</subfield><subfield code="a">DE-898</subfield><subfield code="a">DE-355</subfield><subfield code="a">DE-706</subfield><subfield code="a">DE-20</subfield><subfield code="a">DE-1102</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Schmitt, Alexandra</subfield><subfield code="d">ca. 21. Jh</subfield><subfield code="e">Verfasser</subfield><subfield code="0">(DE-588)1098195515</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Role of Peroxiredoxin 6 in human melanoma</subfield><subfield code="b">= Die Funktion von Peroxiredoxin 6 im humanen Melanom</subfield><subfield code="c">vorgelegt von Alexandra Schmitt aus Würzburg</subfield></datafield><datafield tag="246" ind1="1" ind2="1"><subfield code="a">Die Funktion von Peroxiredoxin 6 im humanen Melanom</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="a">Würzburg</subfield><subfield code="c">2015</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">94 Blatt</subfield><subfield code="b">Illustrationen, Diagramme</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="b">n</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="b">nc</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="502" ind1=" " ind2=" "><subfield code="b">Dissertation</subfield><subfield code="c">Bayerische Julius-Maximilians-Universität Würzburg</subfield><subfield code="d">2015</subfield></datafield><datafield tag="546" ind1=" " ind2=" "><subfield code="a">Zusammenfassung in deutscher und englischer Sprache</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Prostaglandin E2</subfield><subfield code="0">(DE-588)4175971-0</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Peroxiredoxin</subfield><subfield code="0">(DE-588)1028994311</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Arachidonsäure</subfield><subfield code="0">(DE-588)4142846-8</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Melanom</subfield><subfield code="0">(DE-588)4074707-4</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="655" ind1=" " ind2="7"><subfield code="0">(DE-588)4113937-9</subfield><subfield code="a">Hochschulschrift</subfield><subfield code="2">gnd-content</subfield></datafield><datafield tag="689" ind1="0" ind2="0"><subfield code="a">Peroxiredoxin</subfield><subfield code="0">(DE-588)1028994311</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2="1"><subfield code="a">Melanom</subfield><subfield code="0">(DE-588)4074707-4</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2="2"><subfield code="a">Arachidonsäure</subfield><subfield code="0">(DE-588)4142846-8</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2="3"><subfield code="a">Prostaglandin E2</subfield><subfield code="0">(DE-588)4175971-0</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2=" "><subfield code="5">DE-604</subfield></datafield><datafield tag="776" ind1="0" ind2="8"><subfield code="i">Erscheint auch als</subfield><subfield code="n">Online-Ausgabe</subfield><subfield code="o">urn:nbn:de:bvb:20-opus-111465</subfield></datafield><datafield tag="856" ind1="4" ind2="1"><subfield code="u">https://nbn-resolving.org/urn:nbn:de:bvb:20-opus-111465</subfield><subfield code="x">Resolving-System</subfield><subfield code="z">kostenfrei</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="m">DNB Datenaustausch</subfield><subfield code="q">application/pdf</subfield><subfield code="u">http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=028943483&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA</subfield><subfield code="3">Inhaltsverzeichnis</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">ebook</subfield></datafield><datafield tag="999" ind1=" " ind2=" "><subfield code="a">oai:aleph.bib-bvb.de:BVB01-028943483</subfield></datafield></record></collection> |
| genre | (DE-588)4113937-9 Hochschulschrift gnd-content |
| genre_facet | Hochschulschrift |
| id | DE-604.BV043527707 |
| illustrated | Illustrated |
| indexdate | 2024-07-10T07:28:02Z |
| institution | BVB |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-028943483 |
| oclc_num | 951550396 |
| open_access_boolean | 1 |
| owner | DE-384 DE-473 DE-BY-UBG DE-703 DE-1051 DE-824 DE-29 DE-12 DE-91 DE-BY-TUM DE-19 DE-BY-UBM DE-1049 DE-92 DE-739 DE-898 DE-BY-UBR DE-355 DE-BY-UBR DE-706 DE-20 DE-1102 |
| owner_facet | DE-384 DE-473 DE-BY-UBG DE-703 DE-1051 DE-824 DE-29 DE-12 DE-91 DE-BY-TUM DE-19 DE-BY-UBM DE-1049 DE-92 DE-739 DE-898 DE-BY-UBR DE-355 DE-BY-UBR DE-706 DE-20 DE-1102 |
| physical | 94 Blatt Illustrationen, Diagramme |
| psigel | ebook |
| publishDate | 2015 |
| publishDateSearch | 2015 |
| publishDateSort | 2015 |
| record_format | marc |
| spelling | Schmitt, Alexandra ca. 21. Jh Verfasser (DE-588)1098195515 aut Role of Peroxiredoxin 6 in human melanoma = Die Funktion von Peroxiredoxin 6 im humanen Melanom vorgelegt von Alexandra Schmitt aus Würzburg Die Funktion von Peroxiredoxin 6 im humanen Melanom Würzburg 2015 94 Blatt Illustrationen, Diagramme txt rdacontent n rdamedia nc rdacarrier Dissertation Bayerische Julius-Maximilians-Universität Würzburg 2015 Zusammenfassung in deutscher und englischer Sprache Prostaglandin E2 (DE-588)4175971-0 gnd rswk-swf Peroxiredoxin (DE-588)1028994311 gnd rswk-swf Arachidonsäure (DE-588)4142846-8 gnd rswk-swf Melanom (DE-588)4074707-4 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Peroxiredoxin (DE-588)1028994311 s Melanom (DE-588)4074707-4 s Arachidonsäure (DE-588)4142846-8 s Prostaglandin E2 (DE-588)4175971-0 s DE-604 Erscheint auch als Online-Ausgabe urn:nbn:de:bvb:20-opus-111465 https://nbn-resolving.org/urn:nbn:de:bvb:20-opus-111465 Resolving-System kostenfrei Volltext DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=028943483&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Schmitt, Alexandra ca. 21. Jh Role of Peroxiredoxin 6 in human melanoma = Die Funktion von Peroxiredoxin 6 im humanen Melanom Prostaglandin E2 (DE-588)4175971-0 gnd Peroxiredoxin (DE-588)1028994311 gnd Arachidonsäure (DE-588)4142846-8 gnd Melanom (DE-588)4074707-4 gnd |
| subject_GND | (DE-588)4175971-0 (DE-588)1028994311 (DE-588)4142846-8 (DE-588)4074707-4 (DE-588)4113937-9 |
| title | Role of Peroxiredoxin 6 in human melanoma = Die Funktion von Peroxiredoxin 6 im humanen Melanom |
| title_alt | Die Funktion von Peroxiredoxin 6 im humanen Melanom |
| title_auth | Role of Peroxiredoxin 6 in human melanoma = Die Funktion von Peroxiredoxin 6 im humanen Melanom |
| title_exact_search | Role of Peroxiredoxin 6 in human melanoma = Die Funktion von Peroxiredoxin 6 im humanen Melanom |
| title_full | Role of Peroxiredoxin 6 in human melanoma = Die Funktion von Peroxiredoxin 6 im humanen Melanom vorgelegt von Alexandra Schmitt aus Würzburg |
| title_fullStr | Role of Peroxiredoxin 6 in human melanoma = Die Funktion von Peroxiredoxin 6 im humanen Melanom vorgelegt von Alexandra Schmitt aus Würzburg |
| title_full_unstemmed | Role of Peroxiredoxin 6 in human melanoma = Die Funktion von Peroxiredoxin 6 im humanen Melanom vorgelegt von Alexandra Schmitt aus Würzburg |
| title_short | Role of Peroxiredoxin 6 in human melanoma |
| title_sort | role of peroxiredoxin 6 in human melanoma die funktion von peroxiredoxin 6 im humanen melanom |
| title_sub | = Die Funktion von Peroxiredoxin 6 im humanen Melanom |
| topic | Prostaglandin E2 (DE-588)4175971-0 gnd Peroxiredoxin (DE-588)1028994311 gnd Arachidonsäure (DE-588)4142846-8 gnd Melanom (DE-588)4074707-4 gnd |
| topic_facet | Prostaglandin E2 Peroxiredoxin Arachidonsäure Melanom Hochschulschrift |
| url | https://nbn-resolving.org/urn:nbn:de:bvb:20-opus-111465 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=028943483&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT schmittalexandra roleofperoxiredoxin6inhumanmelanomadiefunktionvonperoxiredoxin6imhumanenmelanom AT schmittalexandra diefunktionvonperoxiredoxin6imhumanenmelanom |