Experimental oral infection of goats with Mycobacterium avium subsp. hominissuis: pathomorphological characterization of lesions in different courses of disease with special focus on cellular composition of granulomas
Gespeichert in:
| 1. Verfasser: | |
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| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
Gießen
DVG Service GmbH
2015
|
| Ausgabe: | 1. Auflage |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | 163 Seiten Illustrationen, Diagramme |
| ISBN: | 9783863452889 |
Internformat
MARC
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| 020 | |a 9783863452889 |9 978-3-86345-288-9 | ||
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| 049 | |a DE-188 | ||
| 100 | 1 | |a Schinköthe, Jan |e Verfasser |0 (DE-588)1066825890 |4 aut | |
| 245 | 1 | 0 | |a Experimental oral infection of goats with Mycobacterium avium subsp. hominissuis |b pathomorphological characterization of lesions in different courses of disease with special focus on cellular composition of granulomas |c by Jan Schinköthe |
| 250 | |a 1. Auflage | ||
| 264 | 1 | |a Gießen |b DVG Service GmbH |c 2015 | |
| 300 | |a 163 Seiten |b Illustrationen, Diagramme | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 502 | |b Dissertation |c Tierärztliche Hochschule Hannover |d 2015 | ||
| 650 | 7 | |a goats |2 CAB | |
| 650 | 7 | |a mycobacterium |2 CAB | |
| 650 | 7 | |a granulomas |2 CAB | |
| 650 | 7 | |a mycobacterial diseases |2 CAB | |
| 650 | 7 | |a cellular biology |2 CAB | |
| 650 | 7 | |a goat diseases |2 CAB | |
| 655 | 7 | |0 (DE-588)4113937-9 |a Hochschulschrift |2 gnd-content | |
| 776 | 0 | 8 | |i Erscheint auch als |n Online-Ausgabe |a Schinköthe, Jan |t Experimental oral infection of goats with Mycobacterium avium subsp. hominissuis |
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| 999 | |a oai:aleph.bib-bvb.de:BVB01-028673055 | ||
Datensatz im Suchindex
| _version_ | 1804175768082710528 |
|---|---|
| adam_text | TABLE OF CONTENTS
TABLE OF CONTENTS
11NTRODUCTION 15
2 LITERATURE
REVIEW 17
2.1 MYCOBACTERIUM AVIUM
SUBSPECIES HOMINISSUIS 17
2.1.1 TAXONOMY AND
IDENTIFICATION 17
2.1.2 CHARACTERISTICS AND
ECOLOGY 18
2.2 HOST
RANGE OF MAC
AND PATHOLOGICAL AND
EPIDEMIOLOGICAL FEATURES OF
ASSOCIATED DISEASES 20
2.2.1 HUMANS 20
2.2.2 ANIMALS 23
2.3 REACTIONS OF THE
IMMUNE SYSTEM TO M. AVIUM 25
2.3.1 ENTRY
AT HOST SITES 25
2.3.2 INNATE AND
ADAPTIVE IMMUNE
RESPONSES 26
2.4 EXPERIMENTAL
ANIMAL MODELS IN TB RESEARCH 29
2.4.1 SMALL ANIMAL
MODELS 30
2.4.2 LARGE ANIMAL
MODELS 32
3 MATERIALS
UND
METHODS 35
3.1 EXPERIMENTAL
DESIGN OF THE
ORAL INFECTION
OF GOATS WITH MAH 35
3.1.1 ANIMALS 35
3.1.2 CHARACTERISATION OF THE MAH STRAIN USED
FOR INOCULATION 36
3.1.3 PREPARATION OF THE
INOCULUM 37
3.1.4 STUDY DESIGN AND
INOCULATION 38
3.1.5 NECROPSY AND TISSUE
SAMPLING 41
3.1.6 FIXATION OF SPECIMENS WITH NBF 42
3.1.7 CRYOCONSERVATION 42
3.2 METHODS
USED IN MANUSCRIPT 1 45
3.2.1 MACROSCOPIC
EXAMINATION 45
3.2.2 HISTOLOGICAL EXAMINATION 45
3.2.2.1 PARAFFIN EMBEDDING 45
3.2.2.2 HEMALUM-EOSIN (HE) STAIN 46
3.2.2.3 ANALYSIS OF HE SECTIONS 47
HTTP://D-NB.INFO/1082001546
TABLE OF CONTENTS
3.2.3 IMMUNOHISTOCHEMICAL
(IHC) DETECTION OF MYCOBACTERIA IN FFPE 48
3.2.4 CULTIVATION
OF MAH
IN TISSUE 51
3.3 METHODS USED IN MANUSCRIPT 2 51
3.3.1 SELECTION AND PREPARATION OF TISSUES 51
3.3.2 HE- AND AZAN STAINING OF FROZEN
SECTIONS 52
3.3.3 KINYOUN STAIN 53
3.3.4 IMMUNOHISTOCHEMICAL METHODS
AND ANTIBODIES USED FOR IHC 54
3.3.5 PERFORMING OF IHC 55
3.3.6 IMAGE ANALYSIS OF THE SIZE AND
CELLULAR SUBSETS IN GRANULOMAS 57
4 MANUSCRIPT 1 59
4.1 SUMMARY 60
4.2 INTRODUCTION 60
4.3 MATERIAL AND
METHODS 62
4.4 RESULTS 67
4.5 DISCUSSION 80
5 MANUSCRIPT 2 84
5.1 SUMMARY 85
5.2 INTRODUCTION 85
5.3 MATERIAL
AND METHODS 87
5.3.1 TISSUE SAMPLES 87
5.3.2 STAINING OF FROZEN TISSUE SECTIONS 88
5.3.3 IMMUNOHISTOCHEMISTRY (IHC)
FOR CELL SURFACE MARKERS AND
PROLIFERATION.... 89
5.3.4 IMAGE ANALYSIS
OF THE SIZE
AND CELLULAR SUBSETS
IN GRANULOMAS 90
5.4. RESULTS 92
5.4.1 CELLULAR
COMPOSITION OF GRANULOMAS 92
5.4.1.1 STAGE 1 92
5.4.1.2 STAGE 2 92
5.4.1.3 STAGE 3 93
5.4.1.4 STAGE
4 94
5.4.1.5 STAGE 5 95
5.4.1.6 STAGE 6 95
TABLE OF CONTENTS
5.4.2 DISTRIBUTION
OF GRANULOMA STAGES 1-6
IN OGALT VERSUS ILN
OF GOATS AT 2-3
MPI VERSUS
13 MPI 96
5.4.3 ULCERATIVE AND
GRANULOMATOUS LESIONS
IN OGALT AT 2-3 MPI 97
5.5 DISCUSSION 113
5.6 CONCLUSION 119
6 DISCUSSION 121
6.1 DIFFERENT COURSES
OF DISEASE AND
LESIONS IN GOATS AFTER ORAL
INOCULATION WITH
MAH 121
6.2 CHARACTERIZATION OF MORPHOLOGIC DIFFERENT GRANULOMA STAGES 126
6.3 OUTLOOK AND
POSSIBLE DIRECTIONS OF FURTHER RESEARCH 132
7 SUMMARY 134
8 ZUSAMMENFASSUNG 136
9 REFERENCES 139
10 ANNEX 158
11 ACKNOWLEDGEMENTS 163
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| id | DE-604.BV043250908 |
| illustrated | Illustrated |
| indexdate | 2024-07-10T07:21:32Z |
| institution | BVB |
| isbn | 9783863452889 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-028673055 |
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| physical | 163 Seiten Illustrationen, Diagramme |
| publishDate | 2015 |
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| spelling | Schinköthe, Jan Verfasser (DE-588)1066825890 aut Experimental oral infection of goats with Mycobacterium avium subsp. hominissuis pathomorphological characterization of lesions in different courses of disease with special focus on cellular composition of granulomas by Jan Schinköthe 1. Auflage Gießen DVG Service GmbH 2015 163 Seiten Illustrationen, Diagramme txt rdacontent n rdamedia nc rdacarrier Dissertation Tierärztliche Hochschule Hannover 2015 goats CAB mycobacterium CAB granulomas CAB mycobacterial diseases CAB cellular biology CAB goat diseases CAB (DE-588)4113937-9 Hochschulschrift gnd-content Erscheint auch als Online-Ausgabe Schinköthe, Jan Experimental oral infection of goats with Mycobacterium avium subsp. hominissuis DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=028673055&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Schinköthe, Jan Experimental oral infection of goats with Mycobacterium avium subsp. hominissuis pathomorphological characterization of lesions in different courses of disease with special focus on cellular composition of granulomas goats CAB mycobacterium CAB granulomas CAB mycobacterial diseases CAB cellular biology CAB goat diseases CAB |
| subject_GND | (DE-588)4113937-9 |
| title | Experimental oral infection of goats with Mycobacterium avium subsp. hominissuis pathomorphological characterization of lesions in different courses of disease with special focus on cellular composition of granulomas |
| title_auth | Experimental oral infection of goats with Mycobacterium avium subsp. hominissuis pathomorphological characterization of lesions in different courses of disease with special focus on cellular composition of granulomas |
| title_exact_search | Experimental oral infection of goats with Mycobacterium avium subsp. hominissuis pathomorphological characterization of lesions in different courses of disease with special focus on cellular composition of granulomas |
| title_full | Experimental oral infection of goats with Mycobacterium avium subsp. hominissuis pathomorphological characterization of lesions in different courses of disease with special focus on cellular composition of granulomas by Jan Schinköthe |
| title_fullStr | Experimental oral infection of goats with Mycobacterium avium subsp. hominissuis pathomorphological characterization of lesions in different courses of disease with special focus on cellular composition of granulomas by Jan Schinköthe |
| title_full_unstemmed | Experimental oral infection of goats with Mycobacterium avium subsp. hominissuis pathomorphological characterization of lesions in different courses of disease with special focus on cellular composition of granulomas by Jan Schinköthe |
| title_short | Experimental oral infection of goats with Mycobacterium avium subsp. hominissuis |
| title_sort | experimental oral infection of goats with mycobacterium avium subsp hominissuis pathomorphological characterization of lesions in different courses of disease with special focus on cellular composition of granulomas |
| title_sub | pathomorphological characterization of lesions in different courses of disease with special focus on cellular composition of granulomas |
| topic | goats CAB mycobacterium CAB granulomas CAB mycobacterial diseases CAB cellular biology CAB goat diseases CAB |
| topic_facet | goats mycobacterium granulomas mycobacterial diseases cellular biology goat diseases Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=028673055&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT schinkothejan experimentaloralinfectionofgoatswithmycobacteriumaviumsubsphominissuispathomorphologicalcharacterizationoflesionsindifferentcoursesofdiseasewithspecialfocusoncellularcompositionofgranulomas |