Haemolymph clotting in Drosophila melanogaster and Galleria mellonella:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
2004
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | 131 Bl. Ill., graph. Darst. |
Internformat
MARC
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| 001 | BV026542374 | ||
| 003 | DE-604 | ||
| 005 | 20110228 | ||
| 007 | t| | ||
| 008 | 110326s2004 xx ad|| m||| 00||| eng d | ||
| 015 | |a 05,H05,2076 |2 dnb | ||
| 016 | 7 | |a 974148644 |2 DE-101 | |
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| 040 | |a DE-604 |b ger |e rakwb | ||
| 041 | 0 | |a eng | |
| 049 | |a DE-188 | ||
| 084 | |a 610 |2 sdnb | ||
| 100 | 1 | |a Scherfer, Christoph |e Verfasser |4 aut | |
| 245 | 1 | 0 | |a Haemolymph clotting in Drosophila melanogaster and Galleria mellonella |c submitted by, vorgelegt von Christoph Scherfer |
| 264 | 1 | |c 2004 | |
| 300 | |a 131 Bl. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 502 | |a Gießen, Univ., Diss., 2004 | ||
| 655 | 7 | |0 (DE-588)4113937-9 |a Hochschulschrift |2 gnd-content | |
| 856 | 4 | 2 | |m DNB Datenaustausch |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=022106018&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
| 943 | 1 | |a oai:aleph.bib-bvb.de:BVB01-022106018 | |
Datensatz im Suchindex
| _version_ | 1816445574694567936 |
|---|---|
| adam_text |
TABLE
OF
CONTENTS
1
TABLE
OF
CONTENTS
1
INTRODUCTION
3
1.1
INNATE
AND
ADAPTIVE
IMMUNITY
3
1.2
MODEL
ORGANISMS
USED
IN
THIS
STUDY
3
1.3
HAEMOCYTE
TYPES
IN
G.
MELLONELLA
AND
D.
MELANOGASTER
4
1.4
THE
INSECT
IMMUNE
SYSTEM
6
1.4.1
CUTICLE
AND
PERITROPHIC
MEMBRANE
-
THE
FIRST
LINE
OF
DEFENCE
7
1.4.2
COAGULATION
AND
PHAGOCYTOSIS
-
IMMEDIATE
IMMUNE
REACTIONS
8
1.4.3
MELANISATION
-
IMMOBILISATION
AND
KILLING
OF
PATHOGENS
9
1.4.4
MODULATION,
ENCAPSULATION
AND
WOUND
HEALING
-
STABILISING
THE
SCENE
_
10
1.4.5
INDUCTION
OF
ANTIMICROBIAL
PEPTIDES
12
1.4.6
TOWARDS
AN
INTEGRATED
VIEW
OF
COAGULATION
IN
INNATE
IMMUNITY
13
1.5
COMPARISON
OF
COAGULATION
IN
VERTEBRATES
AND
ARTHROPODS
14
1.5.1
VERTEBRATE
BLOOD
CLOTTING
-
PRIMARY
AND
SECONDAIY
HAEMOSTASIS
14
1.5.2
THE
COAGULATION
CASCADE
OF
HORSESHOE
CRABS
15
1.5.3
HAEMOLYMPH
CLOTTING
IN
CRUSTACEANS
17
1.5.4
INSECT
HAEMOLYMPH
COAGULATION
17
1.6
GLYCOPROTEINS
AND
LECTINS
-
INTERACTIONS
OF
HAEMOCYTES
AND
PATHOGENS
_
20
1.7
HEMOMUCIN,
A
GLYCOPROTEIN
WITH
POSSIBLE
CLOT
FUNCTIONS
22
1.8
PHOSPHOLIPIDS
AND
EICOSANOIDS
-
A
LINK
BETWEEN
APOPTOSIS,
CLOTTING
AND
IMMUNITY?
23
1.9
AIM
OF
THE
PROJECTS
26
2
MATERIALS
AND
METHODS
27
2.1
MAINTENANCE
OF
DROSOPHILA
AND
GALLERIA
CULTURES
27
2.1.1
REARING
CONDITIONS
27
2.1.2
DROSOPHILA
FOOD
MEDIUM
27
2.1.3
GALLERIA
FOOD
MEDIUM
28
2.2
DROSOPHILA
EMBRYO
FIXATION
FOR
IN
SITU
HYBRIDISATION
28
2.3
DNA
MICROINJECTION
OF
DROSOPHILA
EMBRYOS
29
2.4
CROSSINGS
FOR
HEMOLECTIN
RNA
INTERFERENCE
EXPERIMENTS
30
2.5
MAINTENANCE
OF
MBN-2
CELL
CULTURES
31
2.6
COLLECTION
OF
HAEMOLYMPH
AND
HAEMOCYTE
SAMPLES
31
2.7
FLOW
CYTOMETRY
-
FLUORESCENCE-ACTIVATED
CELL
SORTER
(FACS)
32
2.8
IN
SITU
HYBRIDISATION
OF
DROSOPHILA
EMBRYOS
33
2.9
IN
VITRO
TRANSCRIPTION
OF
HEMOMUCIN
RNA
FOR
RNAI
36
2.10
SYNTHESIS
OF
A
DNA
CODING
FOR
A
DSRNA
HAIRPIN
CONSTRUCT
37
TABLE
OF
CONTENTS
2
2.11
RECOMBINANT
EXPRESSION
OF
THE
MOUSE
SSL
PROTEIN
IN
E.
COLI
FOR
PRODUCTION
OF
AN
ANTISERUM
41
2.12
CLOT
ISOLATION
WITH
PARAMAGNETIC
BEADS
IN
THE
PULL-OUT
ASSAY
44
2.13
POLYACRYLAMIDE
SDS
GEL
ELECTROPHORESIS
AND
WESTERN
BLOTS
45
2.14
MASS
SPECTROMETRY
OF
PROTEINS
FROM
THE
PULL-OUT
ASSAY
47
2.15
PREPARATION
OF
TOTAL
RNA
FROM
DROSOPHILA
LARVAE
FOR
RT-PCR
48
2.16
TEST
FOR
WOUND-INDUCED
INDUCTION
OF
HEMOLECTIN
USING
RT-PCR
49
3
RESULTS
50
3.1
STUDIES
ON
LOCALISATION
AND
FUNCTIONS
OF
HEMOMUCIN
50
3.1.1
DETECTION
WITH
HELIX
POMATIA
LECTIN
AND
AN
ANTISERUM
50
3.1.2
IN
SITU
HYBRIDISATION
OF
DROSOPHILA
EMBRYOS
51
3.1.3
STUDIES
ON
MICROPARTICLE
FORMATION
IN
A
HAEMOCYTE-LIKE
CELL
LINE
54
3.1.4
IN
VITRO
TRANSCRIPTION
AND
HEMOMUCIN
RNAI
IN
CELL
CULTURES
56
3.1.5
SYNTHESIS
OF
A
DNA
CONSTRUCT
FOR
HEMOMUCIN
RNAI
IN
VIVO
57
3.2
RECOMBINANT
EXPRESSION
OF
THE
MOUSE
SSL
PROTEIN
IN
E.
COLI
FOR
PRODUCTION
OF
AN
ANTISERUM
59
3.3
USE
OF
A
CLOT-SPECIFIC
ANTISERUM
IN
GALLERIA
MELLONELLA
63
3.4
A
NOVEL
ASSAY
FOR
ISOLATION
OF
CLOT
COMPONENTS
IN
DROSOPHILA
64
3.5
REGULATION
OF
HAEMOLYMPH
COAGULATION
-
PRELIMINARY
RESULTS
70
4
DISCUSSION
73
4.1
STUDIES
ON
LOCALISATION
AND
FUNCTIONS
OF
HEMOMUCIN
73
4.2
RECOMBINANT
EXPRESSION
OF
THE
MOUSE
SSL
PROTEIN
IN
E.
COLI
FOR
PRODUCTION
OF
AN
ANTISERUM
76
4.3
USE
OF
A
CLOT-SPECIFIC
ANTISERUM
IN
GALLERIA
MELLONELLA
78
4.4
A
NOVEL
ASSAY
FOR
ISOLATION
OF
CLOT
COMPONENTS
IN
DROSOPHILA
79
4.5
COMPARISON
OF
CLOTTING
PROTEINS
IN
GALLERIA
AND
DROSOPHILA
89
4.6
REGULATION
OF
HAEMOLYMPH
COAGULATION
-
PRELIMINARY
RESULTS
90
4.7
CONSIDERATIONS
REGARDING
THE
EVOLUTION
OF
CLOTTING
FACTORS
97
5
CONCLUSION
AND
FUTURE
PERSPECTIVES,
ZUSAMMENFASSUNG
UND
ZUKUNFTSPERSPEKTIVEN
102
6
ACKNOWLEDGEMENTS
106
7
REFERENCES
108 |
| any_adam_object | 1 |
| author | Scherfer, Christoph |
| author_facet | Scherfer, Christoph |
| author_role | aut |
| author_sort | Scherfer, Christoph |
| author_variant | c s cs |
| building | Verbundindex |
| bvnumber | BV026542374 |
| ctrlnum | (OCoLC)615360480 (DE-599)BVBBV026542374 |
| discipline | Medizin |
| format | Thesis Book |
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| genre | (DE-588)4113937-9 Hochschulschrift gnd-content |
| genre_facet | Hochschulschrift |
| id | DE-604.BV026542374 |
| illustrated | Illustrated |
| indexdate | 2024-11-22T17:44:50Z |
| institution | BVB |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-022106018 |
| oclc_num | 615360480 |
| open_access_boolean | |
| owner | DE-188 |
| owner_facet | DE-188 |
| physical | 131 Bl. Ill., graph. Darst. |
| publishDate | 2004 |
| publishDateSearch | 2004 |
| publishDateSort | 2004 |
| record_format | marc |
| spelling | Scherfer, Christoph Verfasser aut Haemolymph clotting in Drosophila melanogaster and Galleria mellonella submitted by, vorgelegt von Christoph Scherfer 2004 131 Bl. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Gießen, Univ., Diss., 2004 (DE-588)4113937-9 Hochschulschrift gnd-content DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=022106018&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Scherfer, Christoph Haemolymph clotting in Drosophila melanogaster and Galleria mellonella |
| subject_GND | (DE-588)4113937-9 |
| title | Haemolymph clotting in Drosophila melanogaster and Galleria mellonella |
| title_auth | Haemolymph clotting in Drosophila melanogaster and Galleria mellonella |
| title_exact_search | Haemolymph clotting in Drosophila melanogaster and Galleria mellonella |
| title_full | Haemolymph clotting in Drosophila melanogaster and Galleria mellonella submitted by, vorgelegt von Christoph Scherfer |
| title_fullStr | Haemolymph clotting in Drosophila melanogaster and Galleria mellonella submitted by, vorgelegt von Christoph Scherfer |
| title_full_unstemmed | Haemolymph clotting in Drosophila melanogaster and Galleria mellonella submitted by, vorgelegt von Christoph Scherfer |
| title_short | Haemolymph clotting in Drosophila melanogaster and Galleria mellonella |
| title_sort | haemolymph clotting in drosophila melanogaster and galleria mellonella |
| topic_facet | Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=022106018&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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