Hemorrhagic fever viruses: methods and protocols
This volume presents protocols that analyze and explore hemorrhagic fever viruses (HFV). This book is divided into 5 parts: Part I begins with an overview on predicting viral pandemics and then covers methods for surveillance, diagnosis, and classification of HFV. This includes an antibody capture m...
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Weitere Verfasser: | |
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Format: | Elektronisch E-Book |
Sprache: | English |
Veröffentlicht: |
New York, NY
Springer New York
2018
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Schriftenreihe: | Methods in Molecular Biology
1604 |
Schlagworte: | |
Online-Zugang: | UBR01 TUM01 Volltext |
Zusammenfassung: | This volume presents protocols that analyze and explore hemorrhagic fever viruses (HFV). This book is divided into 5 parts: Part I begins with an overview on predicting viral pandemics and then covers methods for surveillance, diagnosis, and classification of HFV. This includes an antibody capture method using Lassa virus antigens. Part II discusses structural studies and reverse genetics of HFV. The chapters in this part describe envelope glycoprotein membrane fusion studies, arenavirus nucleocapsid protein, and the use of virus-like-particles to study viral egress. Part III explores in vivo models of HFV infections, and contains chapters on murine, guinea pig, and primate models for HFV, and methods to obtain a subset of primary human liver cells that can be cultured long-term. Part IV looks into immune assays and vaccine production for HFV. The chapters in this section cover the attenuated vaccine for Argentine HFV, detecting virus-antibody immune complexes in secondary dengue infections, and DNA vaccination. Part V discusses host responses to viral hemorrhagic fever, and contains chapters on identifying host restrictions to Junín or Dengue infection, and a cell-culture method to assess coagulation after HFV infection. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and cutting-edge, Hemorrhagic Fever Viruses: Methods and Protocols is a valuable resource for scientists and researchers who want to bridge the gap between virus recognition in surveillance and understanding host responses to infection |
Beschreibung: | 1 Online-Ressource (XVI, 425 Seiten) Illustrationen |
ISBN: | 9781493969814 |
DOI: | 10.1007/978-1-4939-6981-4 |
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Datensatz im Suchindex
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any_adam_object | |
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author2_variant | m s s ms mss |
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author_facet | Salvato, Maria S. ca. 20./21. Jh |
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dewey-ones | 616 - Diseases |
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discipline | Medizin |
doi_str_mv | 10.1007/978-1-4939-6981-4 |
format | Electronic eBook |
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indexdate | 2024-07-10T08:05:37Z |
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isbn | 9781493969814 |
language | English |
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physical | 1 Online-Ressource (XVI, 425 Seiten) Illustrationen |
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spelling | Hemorrhagic fever viruses methods and protocols edited by Maria S. Salvato New York, NY Springer New York 2018 1 Online-Ressource (XVI, 425 Seiten) Illustrationen txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology 1604 This volume presents protocols that analyze and explore hemorrhagic fever viruses (HFV). This book is divided into 5 parts: Part I begins with an overview on predicting viral pandemics and then covers methods for surveillance, diagnosis, and classification of HFV. This includes an antibody capture method using Lassa virus antigens. Part II discusses structural studies and reverse genetics of HFV. The chapters in this part describe envelope glycoprotein membrane fusion studies, arenavirus nucleocapsid protein, and the use of virus-like-particles to study viral egress. Part III explores in vivo models of HFV infections, and contains chapters on murine, guinea pig, and primate models for HFV, and methods to obtain a subset of primary human liver cells that can be cultured long-term. Part IV looks into immune assays and vaccine production for HFV. The chapters in this section cover the attenuated vaccine for Argentine HFV, detecting virus-antibody immune complexes in secondary dengue infections, and DNA vaccination. Part V discusses host responses to viral hemorrhagic fever, and contains chapters on identifying host restrictions to Junín or Dengue infection, and a cell-culture method to assess coagulation after HFV infection. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and cutting-edge, Hemorrhagic Fever Viruses: Methods and Protocols is a valuable resource for scientists and researchers who want to bridge the gap between virus recognition in surveillance and understanding host responses to infection Biomedicine Immunology Medicine Salvato, Maria S. ca. 20./21. Jh. (DE-588)1246981114 edt Erscheint auch als Druck-Ausgabe 9781493969807 https://doi.org/10.1007/978-1-4939-6981-4 Verlag URL des Erstveröffentlichers Volltext |
spellingShingle | Hemorrhagic fever viruses methods and protocols Biomedicine Immunology Medicine |
title | Hemorrhagic fever viruses methods and protocols |
title_auth | Hemorrhagic fever viruses methods and protocols |
title_exact_search | Hemorrhagic fever viruses methods and protocols |
title_full | Hemorrhagic fever viruses methods and protocols edited by Maria S. Salvato |
title_fullStr | Hemorrhagic fever viruses methods and protocols edited by Maria S. Salvato |
title_full_unstemmed | Hemorrhagic fever viruses methods and protocols edited by Maria S. Salvato |
title_short | Hemorrhagic fever viruses |
title_sort | hemorrhagic fever viruses methods and protocols |
title_sub | methods and protocols |
topic | Biomedicine Immunology Medicine |
topic_facet | Biomedicine Immunology Medicine |
url | https://doi.org/10.1007/978-1-4939-6981-4 |
work_keys_str_mv | AT salvatomarias hemorrhagicfevervirusesmethodsandprotocols |