Investigation and characterization of N-terminal aminopeptidase activity and degradation signal generation in yeast Saccharomyces cerevisiae:
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| Format: | Abschlussarbeit Buch |
| Sprache: | English |
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Heidelberg
[2023]
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| Beschreibung: | xxi, 105 Seiten Illustrationen, Diagramme 30 cm |
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MARC
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| 100 | 1 | |a Reinbold, Christian |d 1991- |e Verfasser |0 (DE-588)1248660285 |4 aut | |
| 245 | 1 | 0 | |a Investigation and characterization of N-terminal aminopeptidase activity and degradation signal generation in yeast Saccharomyces cerevisiae |c presented by M. Sc. Christian Reinbold |
| 264 | 1 | |a Heidelberg |c [2023] | |
| 300 | |a xxi, 105 Seiten |b Illustrationen, Diagramme |c 30 cm | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 502 | |b Dissertation |c Ruperto Carola University Heidelberg, Germany |d 2023 | ||
| 655 | 7 | |0 (DE-588)4113937-9 |a Hochschulschrift |2 gnd-content | |
| 710 | 2 | |a Universität Heidelberg |0 (DE-588)2024349-2 |4 dgg | |
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| 943 | 1 | |a oai:aleph.bib-bvb.de:BVB01-035113666 | |
Datensatz im Suchindex
| _version_ | 1806142549490925568 |
|---|---|
| adam_text |
CONTENTS
ACKNOWLEDGEMENTS V
CONTRIBUTIONS
VII
PUBLICATIONS
IX
SUMMARY
XI
ZUSAMMENFASSUNG
XIII
ABBREVIATIONS
XV
CONTENTS
XVII
LIST
OF
FIGURES
XIX
LIST
OF
TABLES
XXI
1
INTRODUCTION
1
1.1
CELLULAR
HOMEOSTASIS
.
1
1.1.1
AUTOPHAGY-LYSOSOME
PATHWAY
(ALP)
.
1
1.1.2
THE
UBIQUITIN-PROTEASOME
SYSTEM
(UPS)
.
2
1.2
N-TERMINAL
DEGRADATION
PATHWAYS
.
6
1.2.1
ARG/N-END
PATHWAY
.
6
1.2.2
AC/N-END
PATHWAY
.
8
1.3
N-TERMINAL
ACETYLATION
.
9
1.4
PRECEDING
RESEARCH
RESULTS
.
12
1.5
MOTIVATION
.
15
2
RESULTS
17
2.1
INVESTIGATION
OF
THE
"
MY-PATHWAY
"
.
17
2.2
CONSTRUCTION
OF
HYPOMORPH
ALLELS
OF
NAT
GENES
FOR
FUNCTIONAL
SCREENING
.
.
19
2.3
INVESTIGATION
OF
NOVEL
N-TERMINAL
AMINOPEPTIDASE
ACTIVITY
.
22
XVII
2.3.1
STABILITY
PROFILING
OF
NT-ACETYLATED
NATA/NATB/NATC
REPORTER
LIBRARIES
24
2.3.2
STABILITY
PROFILING
OF
A
NATA
REPORTER
LIBRARY
.
25
2.3.3
STABILITY
PROFILING
OF
A
NATB
REPORTER
LIBRARY
.
27
2.3.4
STABILITY
PROFILING
OF
A
NATC
REPORTER
LIBRARY
.
30
2.3.5
PROTEIN
ABUNDANCES
DO
NOT
CHANGE
IN
NAT
SHUTDOWN
STRAINS
.
32
2.3.6
CONCLUDING
REMARKS
.
33
2.4
APE2/AAPL
SUBSTRATE
SPECIFICITY
IS
NOT
LIMITED
TO
MY-REPORTERS
.
34
2.5
RECOGNITION DETERMINANTS
FOR
N-TERMINAL
UBRI
BINDING
.
38
2.6
REPORTER
TURNOVER
IS
RARELY
CORRELATED
TO
FULL-LENGTH
PROTEIN
TURNOVER
RATES
.
43
2.7
THEN-TERMINUS
OF
SRYL
IS
PROCESSED
BY
APE2/AAPL
.
47
2.8
MISLOCALIZATION
OF
RTTL
05/YERL
04W
DOES
NOT
EFFECT
ITS
STABILITY
.
49
2.9
APE2/AAPL
SUBSTRATES
IN
SOME
CASES
CAN
BE
DEGRADED
VIA
THE
SHRED
PATHWAY
51
2.10
NME
BY
APE2/AAPL
IS
NOT
ESSENTIAL
UNDER
STRESS
CONDITIONS
.
53
3
DISCUSSION
55
3.1
IDENTIFICATION
OF
AMINOPEPTIDASES PROCESSING
N-TERMINI
.
55
3.2
PROCESSING
OF
N-TERMINI
BY
APE2/AAPL
.
58
3.3
SYSTEMATIC
ANALYSIS
OF
REQUIREMENTS
FOR
UBRI
SUBSTRATE
RECOGNITION
.
61
3.4
PHYSIOLOGICAL
ROLE
OF
N-TERMINAL
METHIONINE
EXCISION
.
63
3.5
CONCLUSION
AND
OUTLOOK
.
64
4
MATERIALS
&
METHODS
65
4.1
MATERIALS
.
65
4.1.1
ANTIBODIES
.
65
4.1.2
RESTRICTION
ENZYMES
.
65
4.1.3
OLIGOS
.
66
4.1.4
YEAST
STRAINS
.
67
4.1.5
PLASMIDS
.
68
4.1.6
BUFFERS
&
MEDIUM
.
69
4.2
METHODS
.
71
4.2.1
MOLECULAR
BIOLOGICAL
METHODS
.
71
4.2.2
BIOCHEMICAL
METHODS
.
75
BIBLIOGRAPHY
77
SUPPLEMENTARY
MATERIAL
95 |
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| author | Reinbold, Christian 1991- |
| author_GND | (DE-588)1248660285 |
| author_facet | Reinbold, Christian 1991- |
| author_role | aut |
| author_sort | Reinbold, Christian 1991- |
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| building | Verbundindex |
| bvnumber | BV049772505 |
| ctrlnum | (OCoLC)1376529187 (DE-599)KXP1843289334 |
| format | Thesis Book |
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| illustrated | Illustrated |
| indexdate | 2024-08-01T00:22:40Z |
| institution | BVB |
| institution_GND | (DE-588)2024349-2 |
| language | English |
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| owner_facet | DE-355 DE-BY-UBR |
| physical | xxi, 105 Seiten Illustrationen, Diagramme 30 cm |
| publishDate | 2023 |
| publishDateSearch | 2023 |
| publishDateSort | 2023 |
| record_format | marc |
| spelling | Reinbold, Christian 1991- Verfasser (DE-588)1248660285 aut Investigation and characterization of N-terminal aminopeptidase activity and degradation signal generation in yeast Saccharomyces cerevisiae presented by M. Sc. Christian Reinbold Heidelberg [2023] xxi, 105 Seiten Illustrationen, Diagramme 30 cm txt rdacontent n rdamedia nc rdacarrier Dissertation Ruperto Carola University Heidelberg, Germany 2023 (DE-588)4113937-9 Hochschulschrift gnd-content Universität Heidelberg (DE-588)2024349-2 dgg B:DE-101 application/pdf https://d-nb.info/1289693641/04 Inhaltsverzeichnis DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=035113666&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis 1\p dnb 20230809 DE-101 https://d-nb.info/provenance/plan#dnb |
| spellingShingle | Reinbold, Christian 1991- Investigation and characterization of N-terminal aminopeptidase activity and degradation signal generation in yeast Saccharomyces cerevisiae |
| subject_GND | (DE-588)4113937-9 |
| title | Investigation and characterization of N-terminal aminopeptidase activity and degradation signal generation in yeast Saccharomyces cerevisiae |
| title_auth | Investigation and characterization of N-terminal aminopeptidase activity and degradation signal generation in yeast Saccharomyces cerevisiae |
| title_exact_search | Investigation and characterization of N-terminal aminopeptidase activity and degradation signal generation in yeast Saccharomyces cerevisiae |
| title_full | Investigation and characterization of N-terminal aminopeptidase activity and degradation signal generation in yeast Saccharomyces cerevisiae presented by M. Sc. Christian Reinbold |
| title_fullStr | Investigation and characterization of N-terminal aminopeptidase activity and degradation signal generation in yeast Saccharomyces cerevisiae presented by M. Sc. Christian Reinbold |
| title_full_unstemmed | Investigation and characterization of N-terminal aminopeptidase activity and degradation signal generation in yeast Saccharomyces cerevisiae presented by M. Sc. Christian Reinbold |
| title_short | Investigation and characterization of N-terminal aminopeptidase activity and degradation signal generation in yeast Saccharomyces cerevisiae |
| title_sort | investigation and characterization of n terminal aminopeptidase activity and degradation signal generation in yeast saccharomyces cerevisiae |
| topic_facet | Hochschulschrift |
| url | https://d-nb.info/1289693641/04 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=035113666&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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