Investigation of immobilisation-position, role of domains and iron spin-state in cytochrome P450 BM3 electrocatalysis:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Abschlussarbeit Elektronisch Software E-Book |
| Sprache: | English |
| Veröffentlicht: |
Leipzig
[2020?]
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis Inhaltsverzeichnis |
| Beschreibung: | 1 CD-ROM (128 Blätter) Illustrationen 12 cm |
Internformat
MARC
| LEADER | 00000nmm a2200000 c 4500 | ||
|---|---|---|---|
| 001 | BV046898386 | ||
| 003 | DE-604 | ||
| 005 | 00000000000000.0 | ||
| 006 | a m||| 00||| | ||
| 007 | co|uuu---uuuuu | ||
| 008 | 200915s2020 gw |||| q||u| ||||||eng d | ||
| 016 | 7 | |a 1216042071 |2 DE-101 | |
| 035 | |a (OCoLC)1197715334 | ||
| 035 | |a (DE-599)DNB1216042071 | ||
| 040 | |a DE-604 |b ger |e rda | ||
| 041 | 0 | |a eng | |
| 044 | |a gw |c XA-DE | ||
| 049 | |a DE-355 | ||
| 084 | |a 570 |2 sdnb | ||
| 100 | 1 | |a Ott, Florian |d 1990- |e Verfasser |0 (DE-588)1213929709 |4 aut | |
| 245 | 1 | 0 | |a Investigation of immobilisation-position, role of domains and iron spin-state in cytochrome P450 BM3 electrocatalysis |c vorgelegt von Master of Science Florian Ott |
| 264 | 1 | |a Leipzig |c [2020?] | |
| 300 | |a 1 CD-ROM (128 Blätter) |b Illustrationen |c 12 cm | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b c |2 rdamedia | ||
| 338 | |b cd |2 rdacarrier | ||
| 502 | |b Dissertation |c Universität Leipzig |d 2020 | ||
| 655 | 7 | |0 (DE-588)4113937-9 |a Hochschulschrift |2 gnd-content | |
| 655 | 7 | |0 (DE-588)4139307-7 |a CD-ROM |2 gnd-carrier | |
| 856 | 4 | 2 | |m B:DE-101 |q application/pdf |u https://d-nb.info/1216042071/04 |3 Inhaltsverzeichnis |
| 856 | 4 | 2 | |m DNB Datenaustausch |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=032308095&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
| 999 | |a oai:aleph.bib-bvb.de:BVB01-032308095 | ||
| 347 | |a Textdatei |b PDF | ||
Datensatz im Suchindex
| _version_ | 1804181767975862272 |
|---|---|
| adam_text | TABLE
OF
CONTENTS
TABLE
OF
CONTENTS
..........................................................................................................................
2
ABBREVIATIONS
.................................................................................................................................
5
ZUSAMMENFASSUNG
.........................................................................................................................
9
SUMMARY
.......................................................................................................................................
16
1
INTRODUCTION
..........................................................................................................................
22
1.1
A
HIGHLY
ACTIVE
CYTOCHROME
-
P450
BM3
...................................................................
27
1.2
IMMOBILISATION
OF
P450
BM3
.......................................................................................
30
1.3
ELECTRON
SUPPLY
OF
P450
BM3
THROUGH
APPLICATION
OF
ELECTRIC
POTENTIAL
....................
31
1.4
AIM
OF
THIS
THESIS
..........................................................................................................
35
2
MATERIAL
AND
METHODS
........................................................................................................
37
2.1
MATERIAL
.........................................................................................................................
37
2.1.1
SOLID
PHASE
PEPTIDE
SYNTHESIS
(SPPS)
.....................................................................
37
2.1.2
PROTEIN
CHEMISTRY
......................................................................................................
40
2.2
SOLID
PHASE
PEPTIDE
SYNTHESIS
(SPPS)
.........................................................................
45
2.2.1
LIST
OF
PEPTIDES
AND
GENERAL
SYNTHESIS
CONDITIONS
..................................................
45
2.2.2
PEPTIDES
FOR
C-TERMINAL
IMMOBILISATION
OF
P450
BM3
..........................................
46
2.2.3
PEPTIDES
STP
AND
P450_MLINK
.............................................................................
47
2.2.4
LIGLINK
PEPTIDES
.......................................................................................................
48
2.3
PEPTIDE
PURIFICATION
AND
ANALYSIS
................................................................................
48
2.4
CLONING
OF
P450
BM3
VARIANTS
....................................................................................
49
2.4.1
LIST
OF
P450
BM3
VARIANTS
........................................................................................
50
2.4.2
P450
BM3
BMP
AND
BMR
DOMAINS
.......................................................................
50
2.4.3
VARIANTS
P450_EX,
P450_4R
AND
P450_2R
...........................................................
51
2.4.4
VARIANTS
WITH
A
POINT
MUTATION
...............................................................................
52
2.5
EXPRESSION
AND
PURIFICATION
OF
P450
BM3
VARIANTS
..................................................
52
2.5.1
PURIFICATION
OF
HIS-TAGGED
PROTEINS
.........................................................................
53
2.5.2
PURIFICATION
OF
FLAG-TAGGED
PROTEINS
........................................................................
53
2.5.3
PURIFICATION
OF
PROTEINS
WITH
AN
INTEIN-CBD
DOMAIN
.............................................
53
2.5.4
PROTEIN
CHARACTERISATION
WITH
SDS-PAGE,
WESTERN
BLOT
AND
MALDI-MS
...........
53
2.6
CLEAVAGE
OF
PROTEINS
WITH
TEV-PROTEASE
....................................................................
54
2.7
TRYPTIC
DIGEST
.................................................................................................................
54
2.8
CO-SATURATION
SPECTRA
...................................................................................................
54
2.9
BENZYLOXYRESORUFIN-O-DEALKYLATION
ASSAY
(BROD-ASSAY)
........................................
55
2.10
7-HYDROXYCOUMARIN
ASSAY
(7-HC
ASSAY)
....................................................................
55
2.11
NADPH-ASSAY
...............................................................................................................
56
2.12
SURFACE
ELISA
..............................................................................................................
56
2.13
CIRCULAR
DICHROISM
SPECTROSCOPY
(CD-SPECTROSCOPY)
..................................................
57
2.14
SPIN-STATE
MEASUREMENTS
...............................................................................................
57
2.14.1
ELECTRON
PARAMAGNETIC
RESONANCE
(EPR)
MEASUREMENTS
....................................
57
2.14.2
UV/VIS
MEASUREMENTS
.........................................................................................
57
2.15
NATIVE
CHEMICAL
LIGATION
................................................................................................
57
2.16
THIAZOLIDINE
DEPROTECTION
.............................................................................................
58
3
RESULTS
AND
DISCUSSION
.........................................................................................................
59
3.1
PEPTIDE-MEDIATED
SPECIFIC
IMMOBILISATION
OF
A
CATALYTICALLY
ACTIVE
P450
BM3
VARIANT
........................................................................................................................................
59
3.1.1
DESIGN
OF
SURFACE
BINDING
PEPTIDES
.........................................................................
59
3.1.2
PEPTIDE
SYNTHESIS
AND
CHARACTERISATION
....................................................................
60
3.1.3
P450
-
PEPTIDE
LIGATION
CONSTRUCTS
..........................................................................
64
3.1.4
DISCUSSION
.................................................................................................................
66
3.2
PEPTIDE-MEDIATED
IMMOBILISATION
OF
BMP
ON
ITO
ELECTRODES
...................................
69
3.2.1
SYNTHESIS
AND
CHARACTERISATION
OF
A
SMALL
SURFACE-BINDING
PEPTIDE
.........................
69
3.2.2
EXPRESSION
AND
CHARACTERISATION
OF
P450 BM3
PROTEIN
VARIANTS
............................
70
3.2.3
DISCUSSION
.................................................................................................................
73
3.3
LIGATION
OF
P450
BM3
SEGMENTS
INTO
A
MODULAR
IMMOBILISATION
SYSTEM
UTILISING
THE
PEPTIDE
MEDIATED
SPECIFIC
BINDING
............................................................................................
77
3.3.1
LIGATION
STRATEGY
........................................................................................................
77
3.3.2
SYNTHESIS
OF
LIGLINK
PEPTIDES
...................................................................................
78
3.3.3
SYNTHESIS
OF
PEPTIDE
LINKERS
FOR
LIGATION
..................................................................
80
3.3.4
EXPRESSION
OF
TWO
P450
BM3
PROTEIN
SEGMENTS
....................................................
81
3.3.5
EPL
OF
THE
C-TERMINAL
P450
BM3
DOMAIN
..............................................................
83
3.3.6
EPL
OF
THE
N-TERMINAL
P450
BM3
DOMAIN
..............................................................
85
3.3.7
DISCUSSION
.................................................................................................................
89
3.4
INCORPORATION
OF
A
SURFACE
BINDING
SEQUENCE
BETWEEN
THE
DOMAINS
OF
P450
BM3
BY
MOLECULAR
BIOLOGY
......................................................................................................................
94
3.4.1
INTRODUCTION
OF
LINKER
SEQUENCES
INTO
P450
BM3
FOR
EXPRESSION
...........................
94
3.4.2
ACTIVITY
MEASUREMENTS
FOR
EXPRESSED
P450
BM3
VARIANTS
.....................................
95
3.4.3
DISCUSSION
.................................................................................................................
97
3.5
IDENTIFICATION
AND
CHARACTERISATION
OF
MUTANTS
WITH
ENHANCED
ACTIVITY
IN
SOLUTION
AND
ON
ITO
ELECTRODES
......................................................................................................................
99
3.5.1
MUTANT
DESIGN
AND
CHARACTERISATION
..........................................................................
99
3.5.2
ACTIVITY
MEASUREMENTS
OF
P450
VARIANTS
IN
SOLUTION
.............................................
101
3.5.3
SPIN-STATE
MEASUREMENTS
OF
P450
BM3
VARIANTS
...................................................
103
3
3.5.4
ACTIVITY
MEASUREMENTS
ON
AN
ITO
ELECTRODE
SURFACE
...........................................
105
3.5.5
DISCUSSION
...............................................................................................................
106
4
REFERENCES
............................................................................................................................
110
PUBLICATIONS
AND
PRESENTATIONS
................................................................................................
123
CURRICULUM
VITAE
......................................................................................................................
125
SELBSTSTANDIGKEITSERKLARUNG
.....................................................................................................
126
4
|
| adam_txt |
TABLE
OF
CONTENTS
TABLE
OF
CONTENTS
.
2
ABBREVIATIONS
.
5
ZUSAMMENFASSUNG
.
9
SUMMARY
.
16
1
INTRODUCTION
.
22
1.1
A
HIGHLY
ACTIVE
CYTOCHROME
-
P450
BM3
.
27
1.2
IMMOBILISATION
OF
P450
BM3
.
30
1.3
ELECTRON
SUPPLY
OF
P450
BM3
THROUGH
APPLICATION
OF
ELECTRIC
POTENTIAL
.
31
1.4
AIM
OF
THIS
THESIS
.
35
2
MATERIAL
AND
METHODS
.
37
2.1
MATERIAL
.
37
2.1.1
SOLID
PHASE
PEPTIDE
SYNTHESIS
(SPPS)
.
37
2.1.2
PROTEIN
CHEMISTRY
.
40
2.2
SOLID
PHASE
PEPTIDE
SYNTHESIS
(SPPS)
.
45
2.2.1
LIST
OF
PEPTIDES
AND
GENERAL
SYNTHESIS
CONDITIONS
.
45
2.2.2
PEPTIDES
FOR
C-TERMINAL
IMMOBILISATION
OF
P450
BM3
.
46
2.2.3
PEPTIDES
STP
AND
P450_MLINK
.
47
2.2.4
LIGLINK
PEPTIDES
.
48
2.3
PEPTIDE
PURIFICATION
AND
ANALYSIS
.
48
2.4
CLONING
OF
P450
BM3
VARIANTS
.
49
2.4.1
LIST
OF
P450
BM3
VARIANTS
.
50
2.4.2
P450
BM3
BMP
AND
BMR
DOMAINS
.
50
2.4.3
VARIANTS
P450_EX,
P450_4R
AND
P450_2R
.
51
2.4.4
VARIANTS
WITH
A
POINT
MUTATION
.
52
2.5
EXPRESSION
AND
PURIFICATION
OF
P450
BM3
VARIANTS
.
52
2.5.1
PURIFICATION
OF
HIS-TAGGED
PROTEINS
.
53
2.5.2
PURIFICATION
OF
FLAG-TAGGED
PROTEINS
.
53
2.5.3
PURIFICATION
OF
PROTEINS
WITH
AN
INTEIN-CBD
DOMAIN
.
53
2.5.4
PROTEIN
CHARACTERISATION
WITH
SDS-PAGE,
WESTERN
BLOT
AND
MALDI-MS
.
53
2.6
CLEAVAGE
OF
PROTEINS
WITH
TEV-PROTEASE
.
54
2.7
TRYPTIC
DIGEST
.
54
2.8
CO-SATURATION
SPECTRA
.
54
2.9
BENZYLOXYRESORUFIN-O-DEALKYLATION
ASSAY
(BROD-ASSAY)
.
55
2.10
7-HYDROXYCOUMARIN
ASSAY
(7-HC
ASSAY)
.
55
2.11
NADPH-ASSAY
.
56
2.12
SURFACE
ELISA
.
56
2.13
CIRCULAR
DICHROISM
SPECTROSCOPY
(CD-SPECTROSCOPY)
.
57
2.14
SPIN-STATE
MEASUREMENTS
.
57
2.14.1
ELECTRON
PARAMAGNETIC
RESONANCE
(EPR)
MEASUREMENTS
.
57
2.14.2
UV/VIS
MEASUREMENTS
.
57
2.15
NATIVE
CHEMICAL
LIGATION
.
57
2.16
THIAZOLIDINE
DEPROTECTION
.
58
3
RESULTS
AND
DISCUSSION
.
59
3.1
PEPTIDE-MEDIATED
SPECIFIC
IMMOBILISATION
OF
A
CATALYTICALLY
ACTIVE
P450
BM3
VARIANT
.
59
3.1.1
DESIGN
OF
SURFACE
BINDING
PEPTIDES
.
59
3.1.2
PEPTIDE
SYNTHESIS
AND
CHARACTERISATION
.
60
3.1.3
P450
-
PEPTIDE
LIGATION
CONSTRUCTS
.
64
3.1.4
DISCUSSION
.
66
3.2
PEPTIDE-MEDIATED
IMMOBILISATION
OF
BMP
ON
ITO
ELECTRODES
.
69
3.2.1
SYNTHESIS
AND
CHARACTERISATION
OF
A
SMALL
SURFACE-BINDING
PEPTIDE
.
69
3.2.2
EXPRESSION
AND
CHARACTERISATION
OF
P450 BM3
PROTEIN
VARIANTS
.
70
3.2.3
DISCUSSION
.
73
3.3
LIGATION
OF
P450
BM3
SEGMENTS
INTO
A
MODULAR
IMMOBILISATION
SYSTEM
UTILISING
THE
PEPTIDE
MEDIATED
SPECIFIC
BINDING
.
77
3.3.1
LIGATION
STRATEGY
.
77
3.3.2
SYNTHESIS
OF
LIGLINK
PEPTIDES
.
78
3.3.3
SYNTHESIS
OF
PEPTIDE
LINKERS
FOR
LIGATION
.
80
3.3.4
EXPRESSION
OF
TWO
P450
BM3
PROTEIN
SEGMENTS
.
81
3.3.5
EPL
OF
THE
C-TERMINAL
P450
BM3
DOMAIN
.
83
3.3.6
EPL
OF
THE
N-TERMINAL
P450
BM3
DOMAIN
.
85
3.3.7
DISCUSSION
.
89
3.4
INCORPORATION
OF
A
SURFACE
BINDING
SEQUENCE
BETWEEN
THE
DOMAINS
OF
P450
BM3
BY
MOLECULAR
BIOLOGY
.
94
3.4.1
INTRODUCTION
OF
LINKER
SEQUENCES
INTO
P450
BM3
FOR
EXPRESSION
.
94
3.4.2
ACTIVITY
MEASUREMENTS
FOR
EXPRESSED
P450
BM3
VARIANTS
.
95
3.4.3
DISCUSSION
.
97
3.5
IDENTIFICATION
AND
CHARACTERISATION
OF
MUTANTS
WITH
ENHANCED
ACTIVITY
IN
SOLUTION
AND
ON
ITO
ELECTRODES
.
99
3.5.1
MUTANT
DESIGN
AND
CHARACTERISATION
.
99
3.5.2
ACTIVITY
MEASUREMENTS
OF
P450
VARIANTS
IN
SOLUTION
.
101
3.5.3
SPIN-STATE
MEASUREMENTS
OF
P450
BM3
VARIANTS
.
103
3
3.5.4
ACTIVITY
MEASUREMENTS
ON
AN
ITO
ELECTRODE
SURFACE
.
105
3.5.5
DISCUSSION
.
106
4
REFERENCES
.
110
PUBLICATIONS
AND
PRESENTATIONS
.
123
CURRICULUM
VITAE
.
125
SELBSTSTANDIGKEITSERKLARUNG
.
126
4 |
| any_adam_object | 1 |
| any_adam_object_boolean | 1 |
| author | Ott, Florian 1990- |
| author_GND | (DE-588)1213929709 |
| author_facet | Ott, Florian 1990- |
| author_role | aut |
| author_sort | Ott, Florian 1990- |
| author_variant | f o fo |
| building | Verbundindex |
| bvnumber | BV046898386 |
| ctrlnum | (OCoLC)1197715334 (DE-599)DNB1216042071 |
| discipline | Biologie |
| discipline_str_mv | Biologie |
| format | Thesis Electronic Software eBook |
| fullrecord | <?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01483nmm a2200361 c 4500</leader><controlfield tag="001">BV046898386</controlfield><controlfield tag="003">DE-604</controlfield><controlfield tag="005">00000000000000.0</controlfield><controlfield tag="006">a m||| 00||| </controlfield><controlfield tag="007">co|uuu---uuuuu</controlfield><controlfield tag="008">200915s2020 gw |||| q||u| ||||||eng d</controlfield><datafield tag="016" ind1="7" ind2=" "><subfield code="a">1216042071</subfield><subfield code="2">DE-101</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(OCoLC)1197715334</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)DNB1216042071</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-604</subfield><subfield code="b">ger</subfield><subfield code="e">rda</subfield></datafield><datafield tag="041" ind1="0" ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="044" ind1=" " ind2=" "><subfield code="a">gw</subfield><subfield code="c">XA-DE</subfield></datafield><datafield tag="049" ind1=" " ind2=" "><subfield code="a">DE-355</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">570</subfield><subfield code="2">sdnb</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Ott, Florian</subfield><subfield code="d">1990-</subfield><subfield code="e">Verfasser</subfield><subfield code="0">(DE-588)1213929709</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Investigation of immobilisation-position, role of domains and iron spin-state in cytochrome P450 BM3 electrocatalysis</subfield><subfield code="c">vorgelegt von Master of Science Florian Ott</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="a">Leipzig</subfield><subfield code="c">[2020?]</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">1 CD-ROM (128 Blätter)</subfield><subfield code="b">Illustrationen</subfield><subfield code="c">12 cm</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="b">cd</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="502" ind1=" " ind2=" "><subfield code="b">Dissertation</subfield><subfield code="c">Universität Leipzig</subfield><subfield code="d">2020</subfield></datafield><datafield tag="655" ind1=" " ind2="7"><subfield code="0">(DE-588)4113937-9</subfield><subfield code="a">Hochschulschrift</subfield><subfield code="2">gnd-content</subfield></datafield><datafield tag="655" ind1=" " ind2="7"><subfield code="0">(DE-588)4139307-7</subfield><subfield code="a">CD-ROM</subfield><subfield code="2">gnd-carrier</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="m">B:DE-101</subfield><subfield code="q">application/pdf</subfield><subfield code="u">https://d-nb.info/1216042071/04</subfield><subfield code="3">Inhaltsverzeichnis</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="m">DNB Datenaustausch</subfield><subfield code="q">application/pdf</subfield><subfield code="u">http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=032308095&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA</subfield><subfield code="3">Inhaltsverzeichnis</subfield></datafield><datafield tag="999" ind1=" " ind2=" "><subfield code="a">oai:aleph.bib-bvb.de:BVB01-032308095</subfield></datafield><datafield tag="347" ind1=" " ind2=" "><subfield code="a">Textdatei</subfield><subfield code="b">PDF</subfield></datafield></record></collection> |
| genre | (DE-588)4113937-9 Hochschulschrift gnd-content (DE-588)4139307-7 CD-ROM gnd-carrier |
| genre_facet | Hochschulschrift CD-ROM |
| id | DE-604.BV046898386 |
| illustrated | Not Illustrated |
| index_date | 2024-07-03T15:23:19Z |
| indexdate | 2024-07-10T08:56:54Z |
| institution | BVB |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-032308095 |
| oclc_num | 1197715334 |
| open_access_boolean | |
| owner | DE-355 DE-BY-UBR |
| owner_facet | DE-355 DE-BY-UBR |
| physical | 1 CD-ROM (128 Blätter) Illustrationen 12 cm |
| publishDate | 2020 |
| publishDateSearch | 2020 |
| publishDateSort | 2020 |
| record_format | marc |
| spelling | Ott, Florian 1990- Verfasser (DE-588)1213929709 aut Investigation of immobilisation-position, role of domains and iron spin-state in cytochrome P450 BM3 electrocatalysis vorgelegt von Master of Science Florian Ott Leipzig [2020?] 1 CD-ROM (128 Blätter) Illustrationen 12 cm txt rdacontent c rdamedia cd rdacarrier Dissertation Universität Leipzig 2020 (DE-588)4113937-9 Hochschulschrift gnd-content (DE-588)4139307-7 CD-ROM gnd-carrier B:DE-101 application/pdf https://d-nb.info/1216042071/04 Inhaltsverzeichnis DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=032308095&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis Textdatei PDF |
| spellingShingle | Ott, Florian 1990- Investigation of immobilisation-position, role of domains and iron spin-state in cytochrome P450 BM3 electrocatalysis |
| subject_GND | (DE-588)4113937-9 (DE-588)4139307-7 |
| title | Investigation of immobilisation-position, role of domains and iron spin-state in cytochrome P450 BM3 electrocatalysis |
| title_auth | Investigation of immobilisation-position, role of domains and iron spin-state in cytochrome P450 BM3 electrocatalysis |
| title_exact_search | Investigation of immobilisation-position, role of domains and iron spin-state in cytochrome P450 BM3 electrocatalysis |
| title_exact_search_txtP | Investigation of immobilisation-position, role of domains and iron spin-state in cytochrome P450 BM3 electrocatalysis |
| title_full | Investigation of immobilisation-position, role of domains and iron spin-state in cytochrome P450 BM3 electrocatalysis vorgelegt von Master of Science Florian Ott |
| title_fullStr | Investigation of immobilisation-position, role of domains and iron spin-state in cytochrome P450 BM3 electrocatalysis vorgelegt von Master of Science Florian Ott |
| title_full_unstemmed | Investigation of immobilisation-position, role of domains and iron spin-state in cytochrome P450 BM3 electrocatalysis vorgelegt von Master of Science Florian Ott |
| title_short | Investigation of immobilisation-position, role of domains and iron spin-state in cytochrome P450 BM3 electrocatalysis |
| title_sort | investigation of immobilisation position role of domains and iron spin state in cytochrome p450 bm3 electrocatalysis |
| topic_facet | Hochschulschrift CD-ROM |
| url | https://d-nb.info/1216042071/04 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=032308095&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT ottflorian investigationofimmobilisationpositionroleofdomainsandironspinstateincytochromep450bm3electrocatalysis |
Es ist kein Print-Exemplar vorhanden.
Inhaltsverzeichnis