Antibody Phage Display: Methods and Protocols
Antibody phage display, the definitive technology for monoclonal production, has now advanced to a stage where it can be performed in nonspecialized research laboratories. In Antibody Phage Display: Methods and Protocols, Philippa M. O'Brien and Robert Aitken combine in one volume a comprehensi...
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Weitere Verfasser: | , |
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Format: | Elektronisch E-Book |
Sprache: | English |
Veröffentlicht: |
Totowa, NJ
Humana Press
2002
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Schriftenreihe: | Methods in Molecular Biology™
178 |
Schlagworte: | |
Online-Zugang: | UBR01 TUM01 Volltext |
Zusammenfassung: | Antibody phage display, the definitive technology for monoclonal production, has now advanced to a stage where it can be performed in nonspecialized research laboratories. In Antibody Phage Display: Methods and Protocols, Philippa M. O'Brien and Robert Aitken combine in one volume a comprehensive collection of established antibody phage display protocols, each accompanied by authoritative guidance that will enable the nonspecialist to carry them out successfully. Coverage spans the construction of antibody libraries, the selection of antibody clones with the desired properties, and their modification, expression, and purification. Each readily reproducible method is described by a hands-on expert in step-by-step detail and includes a wealth of practical advice not found in the scientific literature. Extensive notes discuss pitfalls to avoid and offer many possible alternative methods to suit special research situations. An overview by one of the world's leading experts in antibody phage display, Dr. Hennie R. Hoogenboom, surveys the current status of the field and the future of the technology. Comprehensive and highly practical, Antibody Phage Display: Methods and Protocols provides biochemists, molecular biologists, and immunologists with a gold-standard reference guide to the successful isolation, modification, and expression of recombinant antibodies using today's powerful phage display technology |
Beschreibung: | 1 Online-Ressource (XV, 401 p) |
ISBN: | 9781592592401 |
DOI: | 10.1385/1592592406 |
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Datensatz im Suchindex
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any_adam_object | |
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spelling | Antibody Phage Display Methods and Protocols edited by Philippa M. O’Brien, Robert Aitken Totowa, NJ Humana Press 2002 1 Online-Ressource (XV, 401 p) txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology™ 178 Antibody phage display, the definitive technology for monoclonal production, has now advanced to a stage where it can be performed in nonspecialized research laboratories. In Antibody Phage Display: Methods and Protocols, Philippa M. O'Brien and Robert Aitken combine in one volume a comprehensive collection of established antibody phage display protocols, each accompanied by authoritative guidance that will enable the nonspecialist to carry them out successfully. Coverage spans the construction of antibody libraries, the selection of antibody clones with the desired properties, and their modification, expression, and purification. Each readily reproducible method is described by a hands-on expert in step-by-step detail and includes a wealth of practical advice not found in the scientific literature. Extensive notes discuss pitfalls to avoid and offer many possible alternative methods to suit special research situations. An overview by one of the world's leading experts in antibody phage display, Dr. Hennie R. Hoogenboom, surveys the current status of the field and the future of the technology. Comprehensive and highly practical, Antibody Phage Display: Methods and Protocols provides biochemists, molecular biologists, and immunologists with a gold-standard reference guide to the successful isolation, modification, and expression of recombinant antibodies using today's powerful phage display technology Life Sciences Microbiology Life sciences Monoklonaler Antikörper (DE-588)4040094-3 gnd rswk-swf Bakteriophagen (DE-588)4125508-2 gnd rswk-swf Phagen-Display-Bibliothek (DE-588)4512153-9 gnd rswk-swf 1\p (DE-588)4143413-4 Aufsatzsammlung gnd-content Monoklonaler Antikörper (DE-588)4040094-3 s Phagen-Display-Bibliothek (DE-588)4512153-9 s DE-604 Bakteriophagen (DE-588)4125508-2 s 2\p DE-604 O’Brien, Philippa M. edt Aitken, Robert edt Erscheint auch als Druck-Ausgabe 9780896039063 https://doi.org/10.1385/1592592406 Verlag URL des Erstveröffentlichers Volltext 1\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk 2\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk |
spellingShingle | Antibody Phage Display Methods and Protocols Life Sciences Microbiology Life sciences Monoklonaler Antikörper (DE-588)4040094-3 gnd Bakteriophagen (DE-588)4125508-2 gnd Phagen-Display-Bibliothek (DE-588)4512153-9 gnd |
subject_GND | (DE-588)4040094-3 (DE-588)4125508-2 (DE-588)4512153-9 (DE-588)4143413-4 |
title | Antibody Phage Display Methods and Protocols |
title_auth | Antibody Phage Display Methods and Protocols |
title_exact_search | Antibody Phage Display Methods and Protocols |
title_full | Antibody Phage Display Methods and Protocols edited by Philippa M. O’Brien, Robert Aitken |
title_fullStr | Antibody Phage Display Methods and Protocols edited by Philippa M. O’Brien, Robert Aitken |
title_full_unstemmed | Antibody Phage Display Methods and Protocols edited by Philippa M. O’Brien, Robert Aitken |
title_short | Antibody Phage Display |
title_sort | antibody phage display methods and protocols |
title_sub | Methods and Protocols |
topic | Life Sciences Microbiology Life sciences Monoklonaler Antikörper (DE-588)4040094-3 gnd Bakteriophagen (DE-588)4125508-2 gnd Phagen-Display-Bibliothek (DE-588)4512153-9 gnd |
topic_facet | Life Sciences Microbiology Life sciences Monoklonaler Antikörper Bakteriophagen Phagen-Display-Bibliothek Aufsatzsammlung |
url | https://doi.org/10.1385/1592592406 |
work_keys_str_mv | AT obrienphilippam antibodyphagedisplaymethodsandprotocols AT aitkenrobert antibodyphagedisplaymethodsandprotocols |