Modulation of radiosensitivity of human tumor and normal cells by inhibition of heat shock proteins Hsp90 and Hsp70:
Gespeichert in:
| 1. Verfasser: | |
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| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
2013
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| Schlagworte: | |
| Online-Zugang: | Volltext Inhaltsverzeichnis |
| Beschreibung: | VII, 155 Bl. graph. Darst. |
Internformat
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| 100 | 1 | |a Niewidok, Natalia |d 1985- |e Verfasser |0 (DE-588)1044440066 |4 aut | |
| 245 | 1 | 0 | |a Modulation of radiosensitivity of human tumor and normal cells by inhibition of heat shock proteins Hsp90 and Hsp70 |c von Natalia Niewidok |
| 246 | 1 | 3 | |a Modulation der Strahlenempfindlichkeit humaner maligner und nicht-maligner Zellen mittels Inhibition der Hitzeschockproteine Hsp90 und Hsp70 |
| 264 | 1 | |c 2013 | |
| 300 | |a VII, 155 Bl. |b graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 502 | |a Würzburg, Univ., Diss., 2013 | ||
| 546 | |a Zsfassung in dt. und engl. Sprache | ||
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| 650 | 0 | 7 | |a Strahlenbiologie |0 (DE-588)4057819-7 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Hitzeschock-Proteine |0 (DE-588)4255487-1 |2 gnd |9 rswk-swf |
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| 689 | 0 | 1 | |a Hitzeschock-Proteine |0 (DE-588)4255487-1 |D s |
| 689 | 0 | 2 | |a Strahlenbiologie |0 (DE-588)4057819-7 |D s |
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Datensatz im Suchindex
| _version_ | 1804151552222429184 |
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| adam_text | CONTENTS
ABBREVIATIONS IV
1. INTRODUCTION 1
1.1 CELLULAR RESPONSE TO RADIATION-INDUCED DAMAGE 1
1.2 MOLECULARLY TARGETED CHEMORADIOTHERAPY 9
1.3 HEAT SHOCK PROTEIN 90 (HSP90) 11
1.4 HSP90 INHIBITION AS A METHOD FOR SENSITIZING TUMOR CELLS TO
IRRADIATION 15
1.5 HEAT SHOCK PROTEIN 70 (HSP70) 20
1.6 INHIBITION OF HSP70 IN CANCER THERAPY 22
2. OBJECTIVES 25
3. MATERIALS AND
METHODS 28
3.1 MATERIALS 28
3.1.1 ANTIBODIES 28
3.1.2 BUFFERS AND REAGENTS 29
3.1.3 SIRNA SEQUENCES 31
3.1.4 PRIMERS 31
3.1.5 EQUIPMENT 32
3.1.6 OTHERS 32
3.2 METHODS 32
3.2.1 CELL LINES AND CELL CULTURE 32
3.2.2 DRUG TREATMENT 33
3.2.3 X-RAY IRRADIATION 33
3.2.4 CELL VIABILITY ASSAY 34
3.2.5 COLONY SURVIVAL ASSAY 34
3.2.6 CELL LYSATES 35
3.2.7 WESTERN BLOT 35
3.2.8 DETECTION OF HISTONE YH2AX EXPRESSION AND CELL CYCLE PHASE
DISTRIBUTION BY FLOW CYTOMETRY 36
3.2.9 SIRNA TRANSFECTION 36
3.2.10
RT-PCR 38
3.2.11
CELL GROWTH AFTER TRANSFECTION 39
3.2.12
BRDU INCORPORATION ASSAY 39
3.2.13
STATISTICS 40
4. RESULTS 41
4.1 EFFECTS OF NVP-AUY922 AND NVP-BEP800 ON THE RADIATION RESPONSE OF
LUNG CARCINOMA A549 AND GLIOBLASTOMA SNB19 CELL LINES 41
4.1.1 CYTOTOXICITY OF HSP90 INHIBITORS TO TUMOR CELLS 42
4.1.2 COLONY SURVIVAL OF IRRADIATED A549 AND SNB19 CELLS AFTER TREATMENT
WITH NVP-AUY922 AND NVP-BEP800 44
I
HTTP://D-NB.INFO/1044676523
CONTENTS
4.1.3 INFLUENCE OF HSPDO INHIBITION AND IR ON THE EXPRESSION OF HSP90
CLIENTS AND APOPTOTIC MARKER PROTEINS IN TUMOR CELLS 47
4.1.4 EFFECTS OF HSP90 INHIBITION AND IRRADIATION ON THE INDUCTION AND
REPAIR
OF DNA DAMAGE IN A549 AND SNB19 CELLS 57
4.1.5 CHANGES IN THE CELL CYCLE PROGRESSION AFTER HSP90 INHIBITION AND
IRRADIATION IN TUMOR CELLS A549 AND SNB19 62
4.1.6 INFLUENCE OF HSP90 INHIBITION AND IR ON THE EXPRESSION OF CELL
CYCLE-
RELATED PROTEINS IN TUMOR CELL LINES A549 AND SNB19 67
4.2 EFFECTS OF NVP-AUY922 AND NVP-BEP800 ON THE RADIATION RESPONSE OF
NORMAL SKIN FIBROBLAST
STRAINS HFIBL AND HFIB2 70
4.2.1 CYTOTOXICITY OF HSP90 INHIBITORS TO FIBROBLAST CELL LINES 70
4.2.2 COLONY SURVIVAL OF IRRADIATED NORMAL HUMAN FIBROBLAST STRAINS
HFIBL
AND HFIB2 AFTER TREATMENT WITH NVP-AUY922 AND NVP-BEP800 72
4.2.3 INFLUENCE OF HSP90 INHIBITION AND IR ON THE EXPRESSION OF HSP90
CLIENTS AND APOPTOTIC MARKER PROTEINS IN FIBROBLASTS 75
4.2.4 EFFECTS OF HSP90 INHIBITORS AND IRRADIATION ON THE INDUCTION AND
REPAIR
OF DNA DAMAGE IN HFIBL AND HFIB2 CELLS 81
4.2.5 CHANGES IN THE CELL CYCLE PROGRESSION OF FIBROBLAST STRAINS HFIBL
AND
HFIB2 AFTER HSP90 INHIBITION AND IRRADIATION 83
4.2.6 INFLUENCE OF HSP90 INHIBITION AND IR ON THE EXPRESSION OF CELL
CYCLE-
RELATED PROTEINS IN FIBROBLAST STRAINS HFIBL AND HFIB2 90
4.3 INFLUENCE OF HSP70 SILENCING COMBINED WITH NVP-AUY922 ON THE
RADIATION
RESPONSE OF CANCER CELL LINES A549 AND SNB19 93
4.3.1 ANALYSIS OF HEAT SHOCK PROTEINS EXPRESSION AT MRNA AND PROTEIN
LEVEL IN A549 AND SNB19 TUMOR CELL LINES AFTER HSC70 AND HSP70
KNOCK-DOWN 93
4.3.2 INFLUENCE OF HSC70/HSP70 SILENCING ON THE PROLIFERATION OF A549
AND SNB19 CELLS 97
4.3.3 ANALYSIS OF HEAT SHOCK PROTEINS EXPRESSION AT MRNA AND PROTEIN
LEVEL IN A549 AND SNB19 CELLS AFTER COMBINATION OF HSP70 KNOCK
DOWN, NVP-AUY922 ADDITION AND IR 98
4.3.4 COLONY SURVIVAL OF IRRADIATED A549 AND SNB19 CELLS AFTER HSP70
PRE-SILENCING AND TREATMENT WITH NVP-AUY922 102
4.3.5 CHANGES IN THE EXPRESSION OF SEVERAL MARKER PROTEINS AFTER
COMBINED HSP70 KNOCK-DOWN, NVP-AUY922 ADDITION AND
IRRADIATION 104
4.3.6 DNA DAMAGE REPAIR AND CELL CYCLE PROGRESSION AFTER HSP70
SILENCING, NVP-AUY922 ADDITION AND IRRADIATION 109
5 DISCUSSION 117
5.1 EFFECTS OF NVP-AUY922 AND NVP-BEP800 ON THE RADIATION RESPONSE OF
LUNG CARCINOMA A549 AND GLIOBLASTOMA SNB19 CELL LINES 117
5.2 EFFECTS OF NVP-AUY922 AND NVP-BEP800 ON THE RADIATION RESPONSE OF
NORMAL FIBROBLAST STRAINS HFIBL AND HFIB2 123
II
CONTENTS
5.3 INFLUENCE OF HSP70 PRE-SILENCING COMBINED WITH NVP-AUY922 TREATMENT
ON
THE RADIATION RESPONSE OF CANCER CELL LINES A549 AND SNB19 127
6 SUMMARY 130
7 ZUSAMMENFASSUNG 134
8 REFERENCES 138
9 ACKNOWLEDGEMENTS 150
10 CURRICULUM VITAE 152
11 AFFIDAVIT 155
III
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| spelling | Niewidok, Natalia 1985- Verfasser (DE-588)1044440066 aut Modulation of radiosensitivity of human tumor and normal cells by inhibition of heat shock proteins Hsp90 and Hsp70 von Natalia Niewidok Modulation der Strahlenempfindlichkeit humaner maligner und nicht-maligner Zellen mittels Inhibition der Hitzeschockproteine Hsp90 und Hsp70 2013 VII, 155 Bl. graph. Darst. txt rdacontent n rdamedia nc rdacarrier Würzburg, Univ., Diss., 2013 Zsfassung in dt. und engl. Sprache Tumorzelle (DE-588)4186433-5 gnd rswk-swf Strahlenbiologie (DE-588)4057819-7 gnd rswk-swf Hitzeschock-Proteine (DE-588)4255487-1 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Tumorzelle (DE-588)4186433-5 s Hitzeschock-Proteine (DE-588)4255487-1 s Strahlenbiologie (DE-588)4057819-7 s DE-604 Erscheint auch als Online-Ausgabe urn:nbn:de:bvb:20-opus-78728 https://nbn-resolving.org/urn:nbn:de:bvb:20-opus-78728 Resolving-System kostenfrei Volltext DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=026882884&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Niewidok, Natalia 1985- Modulation of radiosensitivity of human tumor and normal cells by inhibition of heat shock proteins Hsp90 and Hsp70 Tumorzelle (DE-588)4186433-5 gnd Strahlenbiologie (DE-588)4057819-7 gnd Hitzeschock-Proteine (DE-588)4255487-1 gnd |
| subject_GND | (DE-588)4186433-5 (DE-588)4057819-7 (DE-588)4255487-1 (DE-588)4113937-9 |
| title | Modulation of radiosensitivity of human tumor and normal cells by inhibition of heat shock proteins Hsp90 and Hsp70 |
| title_alt | Modulation der Strahlenempfindlichkeit humaner maligner und nicht-maligner Zellen mittels Inhibition der Hitzeschockproteine Hsp90 und Hsp70 |
| title_auth | Modulation of radiosensitivity of human tumor and normal cells by inhibition of heat shock proteins Hsp90 and Hsp70 |
| title_exact_search | Modulation of radiosensitivity of human tumor and normal cells by inhibition of heat shock proteins Hsp90 and Hsp70 |
| title_full | Modulation of radiosensitivity of human tumor and normal cells by inhibition of heat shock proteins Hsp90 and Hsp70 von Natalia Niewidok |
| title_fullStr | Modulation of radiosensitivity of human tumor and normal cells by inhibition of heat shock proteins Hsp90 and Hsp70 von Natalia Niewidok |
| title_full_unstemmed | Modulation of radiosensitivity of human tumor and normal cells by inhibition of heat shock proteins Hsp90 and Hsp70 von Natalia Niewidok |
| title_short | Modulation of radiosensitivity of human tumor and normal cells by inhibition of heat shock proteins Hsp90 and Hsp70 |
| title_sort | modulation of radiosensitivity of human tumor and normal cells by inhibition of heat shock proteins hsp90 and hsp70 |
| topic | Tumorzelle (DE-588)4186433-5 gnd Strahlenbiologie (DE-588)4057819-7 gnd Hitzeschock-Proteine (DE-588)4255487-1 gnd |
| topic_facet | Tumorzelle Strahlenbiologie Hitzeschock-Proteine Hochschulschrift |
| url | https://nbn-resolving.org/urn:nbn:de:bvb:20-opus-78728 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=026882884&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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