A manual for primary human cell culture:
Saved in:
| Main Authors: | , |
|---|---|
| Format: | Book |
| Language: | English |
| Published: |
New Jersey [u.a.]
World Scientific
2010
|
| Edition: | 2. ed. |
| Series: | Manuals in biomedical research
6 |
| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Physical Description: | XXIII, 188 S. Ill., graph. Darst. |
| ISBN: | 9789812834775 981283477X |
Staff View
MARC
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| 020 | |a 9789812834775 |9 978-981-283-477-5 | ||
| 020 | |a 981283477X |9 981-283-477-X | ||
| 035 | |a (OCoLC)695619934 | ||
| 035 | |a (DE-599)BVBBV037157126 | ||
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| 100 | 1 | |a Ng, Kee Woei |e Verfasser |4 aut | |
| 245 | 1 | 0 | |a A manual for primary human cell culture |c Kee Woei Ng ; Jan-Thorsten Schantz |
| 250 | |a 2. ed. | ||
| 264 | 1 | |a New Jersey [u.a.] |b World Scientific |c 2010 | |
| 300 | |a XXIII, 188 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 490 | 1 | |a Manuals in biomedical research |v 6 | |
| 650 | 0 | 7 | |a Zellkultur |0 (DE-588)4067547-6 |2 gnd |9 rswk-swf |
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| 700 | 1 | |a Schantz, Jan-Thorsten |d 1969- |e Verfasser |0 (DE-588)118138308 |4 aut | |
| 830 | 0 | |a Manuals in biomedical research |v 6 |w (DE-604)BV023080126 |9 6 | |
| 856 | 4 | 2 | |m Digitalisierung UB Regensburg |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=021071788&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
| 999 | |a oai:aleph.bib-bvb.de:BVB01-021071788 | ||
Record in the Search Index
| _version_ | 1804143757931577344 |
|---|---|
| adam_text | Table
of Contents
Foreword
vii
Foreword
ix
Preface
xi
List of Figures and Tables
xix
A Principles of Biosafety
1
A.I Categories of Biosafety
2
A.2 Biohazard Materials
3
A.3 Recommended Work Practices
3
A.4 General Hygiene
4
A.4.1 Pipettes
5
A.4.2 Syringes and Scalpels
5
A.4.3 Biosafety Cabinets
5
A.4.4 Chemicals
6
A.4.
5
Ultraviolet Light
7
A.4.6 General Housekeeping
7
A.S Handling Biological Specimens
8
A.S.I Checklist
10
В
Introduction to the Cell Culture Laboratory
11
B.I The Laminar Flow Workbench
—
Biosafety Cabinet
12
B.2 The Centrifuge
12
B.3 The Waterbath
15
B.4 The Sink/Wet Space
16
B.5 The Autoclave
16
x¡v
♦
Table of Contents
B.6 The Refrigerator/Freezer
18
Ђ.7
The Incubator
19
B.8 The Microscope
21
Notes
22
С
Protocols for Human Cell Culture
23
C.I Cell Isolation
25
C.2 Mesenchymal Cells
27
C.2.1 Adipose Cells
27
Enzymatic Digestion
28
C.2.
2
Chondrocytes
29
Enzymatic Digestion
29
C.2.3 Endothelial Cells
31
HUVEC (Human Umbilical Vein
Endothelial Cells) Isolation
31
С
2.4
Fibroblasts
32
Explant
Method
33
Enzymatic Digestion
33
Subculturing
34
C.2.
5
Myocytes
35
Skeletal Muscle Cells
35
Enzymatic Digestion
35
Subculturing
36
Smooth Muscle Cells
36
Enzymatic Digestion
37
Subculturing
38
C.2.6 Osteoblasts
38
Osteoblasts
40
Periosteal Ceils
40
Wash-out Method
42
Expiant
Method
43
Enzymatic Digestion
44
C.2..7 Dental Cells (Periodontal Ligament
Fibroblasts
and Alveolar Osteoblasts)
44
Expiant
Culture
45
Notes
48
C.3. Epithelial Cells
51
C.3.1 Respiratory Epithelial Cells
51
Enzymatic Digestion
52
Table
of Contents
♦ xv
С.
3.2
Oral
Mucosa
Cells
53
Explant
Method
54
Subciilturìng
55
C.3.3 Keratinocytes
56
Separating the Epidermis from
Dermis
56
isolating Single Cells from the Epidermis
57
Culturing on
a Fibroblast
Feeder Layer
57
Culturing in Serum-Free Medium
58
Subculturing for Both Feeder-Layer
and Serum-Free
59
C.3.4 Hepatocytes
59
Enzymatic Digestion
60
C.3.5 Kidney Cells
61
Renal Cortical Cells
62
Proximal
Tubuli
Cells
63
Glomernla Mesangial Cells
64
Notes
66
C.4 Bone Marrow Derived Cells
69
C.4.1 Bone Marrow Stromal Cells
69
C.4.2 Peripheral Blood Mononuclear Cells
71
Notes
74
C.5 Stem Cells
77
C.5.
і
Embryonic Stem Cells
77
Culturing Embryonic Stem CelL· Using
Mouse Embryonic
Fibroblasts
as Feeders
77
Culturing Embryonic Stem Cells Using
Human Placenta Cells as Feeders
83
C.5.2 Adult Stem Cells
85
Human Adipose Tissue Derived Stem Cells
85
Human Bone Marrow Derived Stem Cells
90
Human
Amniotic
Epithelial Stem Cells
92
Xotes
95
D
Reagents and Techniques for Cell Culture
97
D.I Selection of Cell Culture Media
98
D.I.I Classical Media
98
D.I
.2
Serum-Free and Speciality Media
102
Notes 105
xvi ♦
Table
of
Contents
D.2
Cell Culture Reagents and Media Supplements
108
D.2.1 Sera
108
Protocol for Isolation of Serum from
a Blood Sample
108
D.2.
2
Antibiotics
(AB)
and Antimycotics (AM)
109
D.2.3 Biological Buffers
112
D.2.
4
Supplements
113
Notes
115
D.3 Cell Dissociation Products
118
D.3.1 General Considerations
118
D.3.
2
Basic Strategy
119
D.3.3
Trypsin
120
D.
3.4
Other Proteases
121
Notes
124
E
Techniques for Manipulating Cell Cultures
and Studying Cellular Behaviour
127
E.I Cryopreservation and Thawing of Cells
128
E.2 Transfection
131
E.2.1 Lipofection
133
E.2.
2
Electroporation
135
E.2.3 Calcium Phosphate Precipitation
137
E.2.
4
Viral Vectors
137
E.2.
5
Generation of Stable Lines
139
E.3 Quantitative Cell Proliferation Assays
140
E.3.1 Cell Counting with the Haemocytometer
141
E.3.2
MTS
Cell Proliferation Assay
144
E.3.3 AlamarBlue® Proliferation Assay
145
E.3.4 WST-1 Proliferation Assay
146
E.3.
5
[3H]-Thymidine Labelling Proliferation
Assay
147
E.3.
6
PicoGreen®
DNA
Cell Proliferation Assay
148
E.3.
7
Use of Cell Proliferation Assays
for High Cell Numbers and
3D
Cultures
149
E.3.
8
Colony Forming Unit
Fibroblast
Assay
(CFU-F)
150
E.3.
9
Growth Kinetics of Cell Populations
151
Table ot Contents
♦ xvii
E.4
Assessment of Gene Expression
153
E.4.1 Transcript Expression
153
Total
RNA
Extraction
153
Genomic
DNA
Digestion
(20
μΐ
Reaction)
154
Reverse Transcription
(20
μΐ
Reaction)
155
Polymerase Chain Reaction
155
Detection of PCR Products
159
E.4.
2
Protein Expression
160
Western Blotting
160
Immunocytochemistry
166
F
Troubleshooting
171
Notes
175
Suggested Readings
179
Abbreviations
181
Index
183
|
| any_adam_object | 1 |
| author | Ng, Kee Woei Schantz, Jan-Thorsten 1969- |
| author_GND | (DE-588)118138308 |
| author_facet | Ng, Kee Woei Schantz, Jan-Thorsten 1969- |
| author_role | aut aut |
| author_sort | Ng, Kee Woei |
| author_variant | k w n kw kwn j t s jts |
| building | Verbundindex |
| bvnumber | BV037157126 |
| classification_rvk | WX 6604 |
| ctrlnum | (OCoLC)695619934 (DE-599)BVBBV037157126 |
| discipline | Biologie |
| edition | 2. ed. |
| format | Book |
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| id | DE-604.BV037157126 |
| illustrated | Illustrated |
| indexdate | 2024-07-09T22:52:44Z |
| institution | BVB |
| isbn | 9789812834775 981283477X |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-021071788 |
| oclc_num | 695619934 |
| open_access_boolean | |
| owner | DE-355 DE-BY-UBR DE-11 |
| owner_facet | DE-355 DE-BY-UBR DE-11 |
| physical | XXIII, 188 S. Ill., graph. Darst. |
| publishDate | 2010 |
| publishDateSearch | 2010 |
| publishDateSort | 2010 |
| publisher | World Scientific |
| record_format | marc |
| series | Manuals in biomedical research |
| series2 | Manuals in biomedical research |
| spelling | Ng, Kee Woei Verfasser aut A manual for primary human cell culture Kee Woei Ng ; Jan-Thorsten Schantz 2. ed. New Jersey [u.a.] World Scientific 2010 XXIII, 188 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Manuals in biomedical research 6 Zellkultur (DE-588)4067547-6 gnd rswk-swf Zellkultur (DE-588)4067547-6 s DE-604 Schantz, Jan-Thorsten 1969- Verfasser (DE-588)118138308 aut Manuals in biomedical research 6 (DE-604)BV023080126 6 Digitalisierung UB Regensburg application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=021071788&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Ng, Kee Woei Schantz, Jan-Thorsten 1969- A manual for primary human cell culture Manuals in biomedical research Zellkultur (DE-588)4067547-6 gnd |
| subject_GND | (DE-588)4067547-6 |
| title | A manual for primary human cell culture |
| title_auth | A manual for primary human cell culture |
| title_exact_search | A manual for primary human cell culture |
| title_full | A manual for primary human cell culture Kee Woei Ng ; Jan-Thorsten Schantz |
| title_fullStr | A manual for primary human cell culture Kee Woei Ng ; Jan-Thorsten Schantz |
| title_full_unstemmed | A manual for primary human cell culture Kee Woei Ng ; Jan-Thorsten Schantz |
| title_short | A manual for primary human cell culture |
| title_sort | a manual for primary human cell culture |
| topic | Zellkultur (DE-588)4067547-6 gnd |
| topic_facet | Zellkultur |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=021071788&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| volume_link | (DE-604)BV023080126 |
| work_keys_str_mv | AT ngkeewoei amanualforprimaryhumancellculture AT schantzjanthorsten amanualforprimaryhumancellculture |