The development of the hybridoma technology created the possibility to obtain unlimited amounts of monoclonal antibodies (mAb) with high specificity and affinity for any target and to introduce mAbs in a wide range of applications; however, the bulky size of mAbs, costly production, and cumbersome e...

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Bibliographic Details

Other Authors:	Saerens, Dirk (Editor), Muyldermans, Serge (Editor)
Format:	Electronic eBook
Language:	English
Published:	Totowa, NJ Humana Press 2012
Series:	Methods in Molecular Biology, Methods and Protocols 911
Subjects:	Biomedicine Immunology Antibodies Medicine Monoklonaler Antikörper Hybridomtechnik Aufsatzsammlung
Online Access:	UBR01 TUM01 Volltext
Summary:	The development of the hybridoma technology created the possibility to obtain unlimited amounts of monoclonal antibodies (mAb) with high specificity and affinity for any target and to introduce mAbs in a wide range of applications; however, the bulky size of mAbs, costly production, and cumbersome engineering hampered regularly their streamlined development in some applications.  In Single Domain Antibodies: Methods and Protocols, expert researchers examine single variable domain antibody fragments, referred to as VH, VL, VHH or VNAR. These fragments are the smallest intact antigen-binding fragments that can be produced recombinantly at low cost.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls
Physical Description:	1 Online-Ressource (XV, 580 p. 89 illus., 35 illus. in color)
ISBN:	9781617799686
DOI:	10.1007/978-1-61779-968-6