Biological attack of acetylated wood:
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| Main Author: | |
|---|---|
| Format: | Book |
| Language: | English |
| Published: |
Göttingen
Cuvillier
2003
|
| Edition: | 1. Aufl. |
| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Item Description: | Zugl.: Göttingen, Univ., Diss., 2003 |
| Physical Description: | 147 S. Ill., graph. Darst. |
| ISBN: | 389873739X |
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| 650 | 4 | |a Wood |x Deterioration | |
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Record in the Search Index
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| adam_text | Titel: Biological attack of acetylated wood
Autor: Mohebby, Behbood
Jahr: 2003
Contents
Chapter 1
Research background
1.1. Introduction ...................................................... 1
1.2. Chemical wood modification ......................................................1
1.2.1. Etherification ...................................................... 2
1.2.2. Esterfication ...................................................... 4
1.2.3. Urethanes ...................................................... 7
1.2.4. Oxidation ......................................................8
1.2.5. Silylation ......................................................8
1.3. Acetylationofwood ...................................................... 10
1.3.1. Acetylation processes ...................................................... 10
1.3.2. Properties of the acetylated wood ...................................................... 11
1.4. Other types of wood modification ...................................................... 16
1.4.1. Thermal wood modification ...................................................... 16
1.4.2. Enzymatic wood modification ...................................................... 18
1.5. Aims of this thesis ...................................................... 19
References ...................................................... 19
Chapter 2
Soil bed trials with acetylated wood
Material and methods ...................................................... 30
2.1. Sample preparation ...................................................... 30
2.2. Acetylation ...................................................... 30
2.3. Soil bed trials ...................................................... 31
2.3.1. Sample preparation ...................................................... 31
2.3.2. Determination of dynamic Modulus of Elasticity (MOEdy,,)..............................31
2.3.3. Soil bed preparation ...................................................... 32
2.3.4. Chemical analysis ...................................................... 33
2.3.4.1. Extractives free wood preparation................................................33
2.3.4.2. Klason lignin ...................................................... 33
2.3.4.3. Holocellulose ...................................................... 33
2.3.4.4. a-Cellulose ...................................................... 34
2.3.4.5. IRspectroscopy ...................................................... 34
2.3.5. Microscopy ...................................................... 35
2.3.5.1. Light microscopy ...................................................... 35
2.3.5.2. Scanning Electron Microscopy...................................................35
2.3.6. Microcalorimetry ...................................................... 35
2.3.7. Ergosterol assay in decayed wood ...................................................... 36
2.4. Results and discussion ...................................................... 37
2.4.1. Mass and MOE*, losses ...................................................... 37
2.4.2. Chemical analysis ...................................................... 40
2.4.3. Microscopy ...................................................... 42
2.4.4. IR spectra of acetylated wood ...................................................... 51
2.4.5. Microcalorimetry ...................................................... 57
2.4.6. Ergosterol assay ...................................................... 58
2.5. General conclusion ...................................................... 61
2.6. Acknowledgements ...................................................... 61
References ...................................................... 61
Chapter 3
Microbial attack of acetylated wood in field soil trials
3.1. Introduction ...................................................... 68
3.2. Material and methods ...................................................... 68
3.2.1. Sample preparation ...................................................... 68
3.2.2. Acetylation ...................................................... 68
3.2.3. Determination of acetyl content ...................................................... 69
3.2.3. Field test of acetylated wood in soil contact.............................................. 69
3.2.5. Microscopy ...................................................... 70
3.2.5.1. Light microscopy ...................................................... 70
3.2.5.2. Scanning Electron Microscopy...................................................70
3.3. Results ...................................................... 70
3.4. Discussion and conclusion ...................................................... 72
References ...................................................... 81
Chapter 4
Degradation of acetylated wood by Basidiomycetes
4.1. Introduction ...................................................... 83
4.2. Material and methods ...................................................... 83
4.2.1. Sample preparation ...................................................... 83
4.2.2. Microorganisms and conditions ...................................................... 83
4.2.3. Microscopy ...................................................... 84
4.2.3.1. Light microscopy ...................................................... 84
4.2.3.2. Scanning Electron Microscopy....................................................84
4.3. Results 84
4.3.1. Trametes versicolor 84
4.3.2. Poria placenta ...................................................... 96
4.4. Discussion HI
References 116
Chapter 5
Bioassays of acetylated wood
5.1. Introduction 119
5.2. Material and methods 119
5.2.1. Sample preparation .............. ................. 119
5.2.2. Sterilization ...................................................... 119
5.2.3. Solid-state fermentation ...................................................... 119
5.2.3.1. Medium and fungus ...................................................... 119
5.2.3.2. Mass loss determination ...................................................... 120
5.2.3.3. Ergosterol assay ...................................................... 120
5.2.3.4. Microcalorimetry ...................................................... 120
5.2.3.5. Fluorescein Diacetate analysis (FDA)..........................................120
5.2.4. Liquid-state fermentation ......................................................121
5.2.4.1. Medium and fungus ......................................................121
5.2.4.2. Protein assay ...................................................... 122
5.2.4.3. MnP assay ...................................................... 122
5.2.4.4. Laccase assay ...................................................... 123
5.2.4.5. Xylanase assay ......................................................123
5.2.4.6. Endoglucanase (EG) (endo-fype|J-l,4-gulcanase) activity ............... 123
5.2.4.7. CBH(exo-typep-l,4-gulcanase) activity .................................... 123
5.2.4.8. DNS assay for reducing sugars...................................................123
5.3. Results and discussion ...................................................... 124
5.3.1. Solid-state fermentation ...................................................... 124
5.3.2. Liquid-state fermentation ...................................................... 129
5.4. Conclusion ...................................................... 133
References ...................................................... 134
Chapter 6
General Discussion
6.1. Acetylation level and protection mechanism ......................................................136
6.2. Chemical aspects of protection in acetylated wood.................................................... 137
6.3. Microscopical evaluation of microbial attack ...................................................... 138
6.4. Fungal protection mechanism and bioassays ...................................................... 139
6.5. Mode of protection in acetylated wood ...................................................... 141
6.6. Conclusion ...................................................... 142
References ...................................................... 143
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| language | English |
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| spelling | Mohebby, Behbood 1966- Verfasser (DE-588)124870899 aut Biological attack of acetylated wood vorgelegt von Behbood Mohebby 1. Aufl. Göttingen Cuvillier 2003 147 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Zugl.: Göttingen, Univ., Diss., 2003 Chemie Wood Chemistry Wood Deterioration Wood Microbiology Wood-decaying fungi Holzschutz (DE-588)4025741-1 gnd rswk-swf Biologischer Abbau (DE-588)4145625-7 gnd rswk-swf Acetylierung (DE-588)4221286-8 gnd rswk-swf Holzzerstörende Pilze (DE-588)4660872-2 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Holzschutz (DE-588)4025741-1 s Acetylierung (DE-588)4221286-8 s Holzzerstörende Pilze (DE-588)4660872-2 s Biologischer Abbau (DE-588)4145625-7 s DE-604 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=010518063&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Mohebby, Behbood 1966- Biological attack of acetylated wood Chemie Wood Chemistry Wood Deterioration Wood Microbiology Wood-decaying fungi Holzschutz (DE-588)4025741-1 gnd Biologischer Abbau (DE-588)4145625-7 gnd Acetylierung (DE-588)4221286-8 gnd Holzzerstörende Pilze (DE-588)4660872-2 gnd |
| subject_GND | (DE-588)4025741-1 (DE-588)4145625-7 (DE-588)4221286-8 (DE-588)4660872-2 (DE-588)4113937-9 |
| title | Biological attack of acetylated wood |
| title_auth | Biological attack of acetylated wood |
| title_exact_search | Biological attack of acetylated wood |
| title_full | Biological attack of acetylated wood vorgelegt von Behbood Mohebby |
| title_fullStr | Biological attack of acetylated wood vorgelegt von Behbood Mohebby |
| title_full_unstemmed | Biological attack of acetylated wood vorgelegt von Behbood Mohebby |
| title_short | Biological attack of acetylated wood |
| title_sort | biological attack of acetylated wood |
| topic | Chemie Wood Chemistry Wood Deterioration Wood Microbiology Wood-decaying fungi Holzschutz (DE-588)4025741-1 gnd Biologischer Abbau (DE-588)4145625-7 gnd Acetylierung (DE-588)4221286-8 gnd Holzzerstörende Pilze (DE-588)4660872-2 gnd |
| topic_facet | Chemie Wood Chemistry Wood Deterioration Wood Microbiology Wood-decaying fungi Holzschutz Biologischer Abbau Acetylierung Holzzerstörende Pilze Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=010518063&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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