The role of extracellular matrix remodeling during ventral nerve cord development in Drosophila melanogaster:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
2013
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| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | III, 114 S. Ill., graph. Darst. |
Internformat
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Datensatz im Suchindex
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| adam_text | IMAGE 1
TABLE O F CONTENTS
1 INTRODUCTION 1
1.1 NERVOUS SYSTEM DEVELOPMENT IN DROSOPHILA 1
1.2 DROSOPHILA GLIA 3
1.3 VENTRAL NERVE CORD CONDENSATION 5
1.4 EXTRACELLULAR MATRIX 7
1.5 INTEGRINS 10
1.6 MATRIX METALLOPROTEASES 11
1.7 THE ADAMS FAMILY AND THEIR RELATIVES 14
1.8 AIM OF THIS WORK 16
2 EXPERIMENTAL PROCEDURES 17
2.1 MATERIALS 17
2.1.1 CHEMICALS AND REAGENTS 17
2.1.2 BUFFER, MEDIA AND SOLUTIONS 17
2.1.3 PLASMIDS 20
2.1.4 ANTIBODIES 21
2.1.5 FLY STRAINS 21
2.1.6 PRIMER 25
2.2 METHODS 26
2.2.1 GENETIC WORK 26
2.2.2 HISTOLOGIC METHODS AND FIXATIONS 27
2.2.3 GENERATION OF MUTATIONS 28
2.2.4 MOLECULAR METHODS 29
2.2.5 BEHAVIOUR TESTS 32
2.2.6 IMAGING 34
HTTP://D-NB.INFO/1033730041
IMAGE 2
TABLE O F CONTENTS
2.2.7 RELATIVE VNC LENGTH DETERMINATION 34
2.2.8 SEQUENCING 34
3 RESULTS 35
3.1 EXTRACELLULAR MATRIX REMODELING MOLECULES ARE REQUIRED FOR VENTRAL
NERVE CORD
DEVELOPMENT 35
3.1.1 INTEGRIN SUBUNITS ARE REQUIRED IN GLIA TO MAINTAIN A NORMAL VNC
SHAPE 35
3.1.2 THE MATRIX METALLOPROTEASE MMP2 IS REQUIRED IN GLIA TO MAINTAIN A
NORMAL
VNC SHAPE 37
3.1.3 EXPRESSION PATTERN OF MMP2 42
3.1.4 THE ELONGATION OF VNC IS CAUSED BY A CHANGE IN CELL SHAPE 42
3.2 ANALYSIS O F THE ROLE O F CG31619 DURING THE VNC DEVELOPMENT 45
3.2.1 PANGLIAL SUPPRESSION OF CG31619 LEADS TO AN ELONGATED VNC 45
3.2.2 GENERATION AND TESTING OF UAS CG31619 CONSTRUCTS 48
3.2.3 LOCALIZATION OF CG31619 49
3.2.4 GENERATION OF A PRECISE DELETION BY FLP/FRT-RECOMBINATION 49
3.2.5 NOLO* 2 10 AFFECTS SURVIVAL RATES DURING EMBRYOGENESIS AND
METAMORPHOSIS 52
3.2.6 HOMOZYGOUS NOLO I2~10 MUTANTS SHOW BEHAVIORAL DEFECTS 54
3.3 ANALYSIS OF CG3811 56
3.3.1 STRUCTURE AND FUNCTION OF CG3811 57
3.3.2 A NEW REPOGAL4 INSERTION CAUSES AN ELONGATED VNC IN THIRD INSTAR
LARVAE 59
3.3.3 GENERATION OF A PRECISE DELETION IN THE OATP30B GENE LOCUS 60
3.3.4 OATP30B I4 14 FLIES SHOW WEAK BEHAVIORAL DEFECTS 64
3.4 A DOUBLE KNOCK-OUT OF NOLO AND OATP3QB DOES NOT RESULT IN ELONGATED
VNCS 65
3.5 THE ECM STRUCTURE AROUND ELONGATED VNCS IS IMPERFECTLY DEVELOPED 65
3.6 ANOTHER METALLOPROTEASE AND THE METALLOPROTEASE INHIBITOR TIMP HAVE
AN EFFECT
ON VNC DEVELOPMENT 67
II
IMAGE 3
TABLE O F CONTENTS
3.7 AN ACTIVATION OF FGF SIGNALING RESULTS IN ELONGATED VNCS 69
3.8 THE OVEREXPRESSION OF MMP2 WITH A NEW GLIA SPECIFIC DRIVERLINE
CAUSES AN
ELONGATED VNC 72
3.9 IDENTIFICATION OF TWO EMS MUTATIONS WHICH CAUSE ELONGATED VNCS 72
4 DISCUSSION 76
4.1 SHAPE CHANGES OF THE VENTRAL NERVE CORD DURING DEVELOPMENT 76
4.2 MOLECULAR PATHWAYS AFFECTING VNC MORPHOGENESIS 77
4.3 THE LEVEL OF GLIAL MMP2 ACTIVITY INFLUENCES THE VNC DEVELOPMENT 78
4.4 INTERACTION OF MMP2 AND INTEGRINS DURING ECM REMODELING 80
4.5 TIMP REGULATES MMP ACTIVITY AND THEREBY MIGHT AFFECT THE CELL - ECM
CONNECTION 81
4.6 NOLO MIGHT HAVE A REGULATORY FUNCTION ON MMP ACTIVITY 81
4.7 A PARTICIPATION OF OATP3C)B ON VNC DEVELOPMENT CANNOT BE PROVEN 82
4.8 THE LOSS OF NOLO AND OATP30B CAUSES BEHAVIORAL DEFECTS 84
5 SUMMARY 86
6 REFERENCES 87
7 APPENDIX 108
7.1 LIST OF ABBREVIATIONS 108
7.2 VECTOR MAP OF PUASTATTB_CG31619 111
7.3 VECTOR MAP OF PUASTATTB_CG31619_GFP 112
7.4 DEPICTION OF NOLO I2 10 113
7.5 DEPICTION OF QATP30B 14 114
III
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| any_adam_object | 1 |
| author | Meyer, Silke Melanie 1978- |
| author_GND | (DE-588)1033636258 |
| author_facet | Meyer, Silke Melanie 1978- |
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| building | Verbundindex |
| bvnumber | BV040980427 |
| ctrlnum | (OCoLC)854701937 (DE-599)HBZHT017614283 |
| dewey-full | 573.8538615774 |
| dewey-hundreds | 500 - Natural sciences and mathematics |
| dewey-ones | 573 - Specific physiological systems in animals |
| dewey-raw | 573.8538615774 |
| dewey-search | 573.8538615774 |
| dewey-sort | 3573.8538615774 |
| dewey-tens | 570 - Biology |
| discipline | Biologie |
| format | Thesis Book |
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| language | English |
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| spelling | Meyer, Silke Melanie 1978- Verfasser (DE-588)1033636258 aut The role of extracellular matrix remodeling during ventral nerve cord development in Drosophila melanogaster vorgelegt von Silke Melanie Meyer 2013 III, 114 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Münster (Westfalen), Univ., Diss., 2013 Strickleiternervensystem (DE-588)4348494-3 gnd rswk-swf Taufliege (DE-588)4078168-9 gnd rswk-swf Extrazelluläre Matrix (DE-588)7530993-2 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Taufliege (DE-588)4078168-9 s Strickleiternervensystem (DE-588)4348494-3 s Extrazelluläre Matrix (DE-588)7530993-2 s DE-604 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025958438&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Meyer, Silke Melanie 1978- The role of extracellular matrix remodeling during ventral nerve cord development in Drosophila melanogaster Strickleiternervensystem (DE-588)4348494-3 gnd Taufliege (DE-588)4078168-9 gnd Extrazelluläre Matrix (DE-588)7530993-2 gnd |
| subject_GND | (DE-588)4348494-3 (DE-588)4078168-9 (DE-588)7530993-2 (DE-588)4113937-9 |
| title | The role of extracellular matrix remodeling during ventral nerve cord development in Drosophila melanogaster |
| title_auth | The role of extracellular matrix remodeling during ventral nerve cord development in Drosophila melanogaster |
| title_exact_search | The role of extracellular matrix remodeling during ventral nerve cord development in Drosophila melanogaster |
| title_full | The role of extracellular matrix remodeling during ventral nerve cord development in Drosophila melanogaster vorgelegt von Silke Melanie Meyer |
| title_fullStr | The role of extracellular matrix remodeling during ventral nerve cord development in Drosophila melanogaster vorgelegt von Silke Melanie Meyer |
| title_full_unstemmed | The role of extracellular matrix remodeling during ventral nerve cord development in Drosophila melanogaster vorgelegt von Silke Melanie Meyer |
| title_short | The role of extracellular matrix remodeling during ventral nerve cord development in Drosophila melanogaster |
| title_sort | the role of extracellular matrix remodeling during ventral nerve cord development in drosophila melanogaster |
| topic | Strickleiternervensystem (DE-588)4348494-3 gnd Taufliege (DE-588)4078168-9 gnd Extrazelluläre Matrix (DE-588)7530993-2 gnd |
| topic_facet | Strickleiternervensystem Taufliege Extrazelluläre Matrix Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025958438&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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