Theranostic tissue engineering: magnetic resonance imaging of biohybrid vascular grafts
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
2014
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | 92, [24] S. Ill., graph. Darst. |
Internformat
MARC
| LEADER | 00000nam a2200000 c 4500 | ||
|---|---|---|---|
| 001 | BV042275776 | ||
| 003 | DE-604 | ||
| 005 | 00000000000000.0 | ||
| 007 | t | ||
| 008 | 150116s2014 ad|| m||| 00||| eng d | ||
| 035 | |a (OCoLC)900641821 | ||
| 035 | |a (DE-599)GBV815300565 | ||
| 040 | |a DE-604 |b ger | ||
| 041 | 0 | |a eng | |
| 049 | |a DE-83 |a DE-355 |a DE-188 | ||
| 082 | 0 | |a 610 | |
| 100 | 1 | |a Mertens, Marianne Elisabeth |d 1984- |e Verfasser |0 (DE-588)1063821797 |4 aut | |
| 245 | 1 | 0 | |a Theranostic tissue engineering |b magnetic resonance imaging of biohybrid vascular grafts |c vorgelegt von Marianne Elisabeth Mertens |
| 264 | 1 | |c 2014 | |
| 300 | |a 92, [24] S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 502 | |a Aachen, Techn. Hochsch., Diss., 2014 | ||
| 546 | |a Zsfassung in engl. und dt. Sprache | ||
| 650 | 0 | 7 | |a Gewebekultur |0 (DE-588)4157245-2 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Kernspintomografie |0 (DE-588)4120806-7 |2 gnd |9 rswk-swf |
| 655 | 7 | |0 (DE-588)4113937-9 |a Hochschulschrift |2 gnd-content | |
| 689 | 0 | 0 | |a Gewebekultur |0 (DE-588)4157245-2 |D s |
| 689 | 0 | 1 | |a Kernspintomografie |0 (DE-588)4120806-7 |D s |
| 689 | 0 | |5 DE-604 | |
| 856 | 4 | 2 | |m DNB Datenaustausch |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027713250&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
| 999 | |a oai:aleph.bib-bvb.de:BVB01-027713250 | ||
Datensatz im Suchindex
| _version_ | 1804152837564792832 |
|---|---|
| adam_text | CONTENTS
1 INTRODUCTION 1
1.1 TISSUE ENGINEERING - HISTORY, PROGRESS, AND CHALLENGES 1
1.2 NON-INVASIVE IMAGING IN TISSUE ENGINEERING 3
1.3 INCORPORATING DIAGNOSTIC NANOPARTICLES INTO IMPLANTS 10
1.4 MAGNETIC RESONANCE IMAGING AND CONTRAST ENHANCEMENT 12
1.5
19
F-MRI AS INNOVATIVE IMAGING CONCEPT 14
1.6 MOTIVATION AND SPECIFIC AIMS OF THIS THESIS 15
2 MATERIAL AND METHODS 18
2.1 USPIO-LABELED COLLAGEN SCAFFOLDS 18
2.1.1 SYNTHESIS OF USPIO 18
2.1.2 SYNTHESIS OF USPIO-NH
2
18
2.1.3 SYNTHESIS OF USPIO-CHO 18
2.1.4 SYNTHESIS OF USPIO-LABELED COLLAGEN SCAFFOLD 19
2.1.5 CHARACTERIZATION OF THE SCAFFOLDS 20
2.1.6 STABILITY TESTS 20
2.1.7 COMPRESSION TESTS 21
2.1.8 MAGNETIC RESONANCE IMAGING 21
2.1.9 PRUSSIAN BLUE STAINING 22
2.1.10 INDUCTIVELY COUPLED PLASMA MASS SPECTROSCOPY ANALYSIS ... 22
2.1.11 COLLAGENASE ASSAY 23
2.1.12 CELL CULTURE 23
2.1.13 SCAFFOLD COLONIZATION, STAINING AND MICROSCOPY 23
2.1.14 IN VIVO EVALUATION 24
2.1.15 EX VIVO ANALYSIS 25
2.1.16 STATISTICAL ANALYSIS 25
2.2 USPIO-LABELED PVDF-BASED VASCULAR GRAFTS 26
2.2.1 SYNTHESIS OF USPIO-LABELED PVDF MESHES AND CHARACTERI
ZATION 26
2.2.2 MAGNETIC RESONANCE IMAGING IN VITRO 26
HTTP://D-NB.INFO/1063114675
2.2.3 BIOCOMPATIBILITY ASSAYS 27
2.2.4 VASCULAR COMPOSITE GRAFT CONSTRUCTION 28
2.2.5 HISTOLOGY 30
2.2.6 IN VIVO EVALUATION 30
2.2.7 EX VIVO ANALYSIS 31
2.2.8 STATISTICS 31
2.3 FLUSPIO-LABELED CELLS IN TISSUE ENGINEERING 32
2.3.1 FLUSPIO SYNTHESIS 32
2.3.2 CELL CULTURE 32
2.3.3 CELL LABELING 33
2.3.4 PRUSSIAN BLUE STAINING 33
2.3.5 IMMUNOHISTOCHEMISTRY AND HISTOLOGY 34
2.3.6 MAGNETIC RESONANCE IMAGING 34
2.3.7 LABEL RETENTION STUDY 35
2.3.8 MTT ASSAY 35
2.3.9 LDH ASSAY 36
2.3.10 ROS ASSAY 36
2.3.11 COLONIZATION OF 3D COLLAGEN SCAFFOLDS 36
2.3.12 VASCULAR GRAFT PREPARATION: CELL ISOLATION, CELL CULTURE AND
FIBRIN SYNTHESIS 37
2.3.13 VASCULAR GRAFT MOLDING: MESH PRODUCTION, GRAFT MOLDING
AND ENDOTHELIALIZATION 38
2.3.14 MR MONITORING DURING BIOREACTOR CULTIVATION 39
2.3.15 MICROSCOPY AND IMMUNOHISTOCHEMICAL ANALYSIS OF THE VAS
CULAR GRAFTS 39
2.3.16 STATISTICAL ANALYSIS 40
2.4
19
F-MRI PROBES FOR IMPLANT LABELING 40
2.4.1 CHEMICALS AND EQUIPMENT 40
2.4.2 INVERSION RECOVERY (DETERMINATION OF TL) 41
2.4.3 HAHN ECHO (DETERMINATION OF T2) 41
2.4.4 PRODUCTION OF
10
F-TPU-BASED BRAIDED STENTS 41
2.4.5 IMAGING OF
19
F-TPU 42
3 RESULTS AND DISCUSSION 43
3.1 USPIO INCORPORATION IN COLLAGEN-BASED SCAFFOLDS 43
3.2 USPIO INCORPORATION IN PVDF-BASED VASCULAR GRAFTS 56
3.3 FLUSPIO-LABELED CELLS IN TISSUE ENGINEERING 66
3.4 EVALUATION OF
19
F-MRI PROBES FOR IMPLANT LABELING 79
4 CONCLUSION AND FUTURE PROSPECTS 88
5 LITERATURE 93
6 CURRICULUM VITAE 111
7 PUBLICATIONS AND PRESENTATIONS 114
|
| any_adam_object | 1 |
| author | Mertens, Marianne Elisabeth 1984- |
| author_GND | (DE-588)1063821797 |
| author_facet | Mertens, Marianne Elisabeth 1984- |
| author_role | aut |
| author_sort | Mertens, Marianne Elisabeth 1984- |
| author_variant | m e m me mem |
| building | Verbundindex |
| bvnumber | BV042275776 |
| ctrlnum | (OCoLC)900641821 (DE-599)GBV815300565 |
| dewey-full | 610 |
| dewey-hundreds | 600 - Technology (Applied sciences) |
| dewey-ones | 610 - Medicine and health |
| dewey-raw | 610 |
| dewey-search | 610 |
| dewey-sort | 3610 |
| dewey-tens | 610 - Medicine and health |
| discipline | Medizin |
| format | Thesis Book |
| fullrecord | <?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01465nam a2200361 c 4500</leader><controlfield tag="001">BV042275776</controlfield><controlfield tag="003">DE-604</controlfield><controlfield tag="005">00000000000000.0</controlfield><controlfield tag="007">t</controlfield><controlfield tag="008">150116s2014 ad|| m||| 00||| eng d</controlfield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(OCoLC)900641821</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)GBV815300565</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-604</subfield><subfield code="b">ger</subfield></datafield><datafield tag="041" ind1="0" ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="049" ind1=" " ind2=" "><subfield code="a">DE-83</subfield><subfield code="a">DE-355</subfield><subfield code="a">DE-188</subfield></datafield><datafield tag="082" ind1="0" ind2=" "><subfield code="a">610</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Mertens, Marianne Elisabeth</subfield><subfield code="d">1984-</subfield><subfield code="e">Verfasser</subfield><subfield code="0">(DE-588)1063821797</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Theranostic tissue engineering</subfield><subfield code="b">magnetic resonance imaging of biohybrid vascular grafts</subfield><subfield code="c">vorgelegt von Marianne Elisabeth Mertens</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2014</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">92, [24] S.</subfield><subfield code="b">Ill., graph. Darst.</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="b">n</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="b">nc</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="502" ind1=" " ind2=" "><subfield code="a">Aachen, Techn. Hochsch., Diss., 2014</subfield></datafield><datafield tag="546" ind1=" " ind2=" "><subfield code="a">Zsfassung in engl. und dt. Sprache</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Gewebekultur</subfield><subfield code="0">(DE-588)4157245-2</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Kernspintomografie</subfield><subfield code="0">(DE-588)4120806-7</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="655" ind1=" " ind2="7"><subfield code="0">(DE-588)4113937-9</subfield><subfield code="a">Hochschulschrift</subfield><subfield code="2">gnd-content</subfield></datafield><datafield tag="689" ind1="0" ind2="0"><subfield code="a">Gewebekultur</subfield><subfield code="0">(DE-588)4157245-2</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2="1"><subfield code="a">Kernspintomografie</subfield><subfield code="0">(DE-588)4120806-7</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2=" "><subfield code="5">DE-604</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="m">DNB Datenaustausch</subfield><subfield code="q">application/pdf</subfield><subfield code="u">http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027713250&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA</subfield><subfield code="3">Inhaltsverzeichnis</subfield></datafield><datafield tag="999" ind1=" " ind2=" "><subfield code="a">oai:aleph.bib-bvb.de:BVB01-027713250</subfield></datafield></record></collection> |
| genre | (DE-588)4113937-9 Hochschulschrift gnd-content |
| genre_facet | Hochschulschrift |
| id | DE-604.BV042275776 |
| illustrated | Illustrated |
| indexdate | 2024-07-10T01:17:04Z |
| institution | BVB |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-027713250 |
| oclc_num | 900641821 |
| open_access_boolean | |
| owner | DE-83 DE-355 DE-BY-UBR DE-188 |
| owner_facet | DE-83 DE-355 DE-BY-UBR DE-188 |
| physical | 92, [24] S. Ill., graph. Darst. |
| publishDate | 2014 |
| publishDateSearch | 2014 |
| publishDateSort | 2014 |
| record_format | marc |
| spelling | Mertens, Marianne Elisabeth 1984- Verfasser (DE-588)1063821797 aut Theranostic tissue engineering magnetic resonance imaging of biohybrid vascular grafts vorgelegt von Marianne Elisabeth Mertens 2014 92, [24] S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Aachen, Techn. Hochsch., Diss., 2014 Zsfassung in engl. und dt. Sprache Gewebekultur (DE-588)4157245-2 gnd rswk-swf Kernspintomografie (DE-588)4120806-7 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Gewebekultur (DE-588)4157245-2 s Kernspintomografie (DE-588)4120806-7 s DE-604 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027713250&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Mertens, Marianne Elisabeth 1984- Theranostic tissue engineering magnetic resonance imaging of biohybrid vascular grafts Gewebekultur (DE-588)4157245-2 gnd Kernspintomografie (DE-588)4120806-7 gnd |
| subject_GND | (DE-588)4157245-2 (DE-588)4120806-7 (DE-588)4113937-9 |
| title | Theranostic tissue engineering magnetic resonance imaging of biohybrid vascular grafts |
| title_auth | Theranostic tissue engineering magnetic resonance imaging of biohybrid vascular grafts |
| title_exact_search | Theranostic tissue engineering magnetic resonance imaging of biohybrid vascular grafts |
| title_full | Theranostic tissue engineering magnetic resonance imaging of biohybrid vascular grafts vorgelegt von Marianne Elisabeth Mertens |
| title_fullStr | Theranostic tissue engineering magnetic resonance imaging of biohybrid vascular grafts vorgelegt von Marianne Elisabeth Mertens |
| title_full_unstemmed | Theranostic tissue engineering magnetic resonance imaging of biohybrid vascular grafts vorgelegt von Marianne Elisabeth Mertens |
| title_short | Theranostic tissue engineering |
| title_sort | theranostic tissue engineering magnetic resonance imaging of biohybrid vascular grafts |
| title_sub | magnetic resonance imaging of biohybrid vascular grafts |
| topic | Gewebekultur (DE-588)4157245-2 gnd Kernspintomografie (DE-588)4120806-7 gnd |
| topic_facet | Gewebekultur Kernspintomografie Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027713250&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT mertensmarianneelisabeth theranostictissueengineeringmagneticresonanceimagingofbiohybridvasculargrafts |